Scnn1g

Functionⁱ

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.By similarity1 Publication

Activity regulationⁱ

Activated by WNK1, WNK2, WNK3 and WNK4.By similarity

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

multicellular organismal water homeostasis Source: UniProtKB
response to hypoxia
Source: RGD
Inferred from expression patternⁱ
- 12372821
sensory perception of taste Source: UniProtKB-KW
sodium ion homeostasis Source: UniProtKB
sodium ion transport
Source: RGD
Inferred from direct assayⁱ
- 16554417
wound healing, spreading of epidermal cells
Source: RGD
Inferred from mutant phenotypeⁱ
- 16844921

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ion channel, Sodium channel
Biological process	Ion transport, Sensory transduction, Sodium transport, Taste, Transport
Ligand	Sodium

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Amiloride-sensitive sodium channel subunit gamma Alternative name(s): Epithelial Na(+) channel subunit gamma Short name: Gamma-ENaC Gamma-NaCH Nonvoltage-gated sodium channel 1 subunit gamma SCNEG
Gene namesⁱ	Name:Scnn1g
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	3641 Scnn1g

RGDⁱ

3641 Scnn1g

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 55	CytoplasmicBy similarityAdd BLAST	55
Transmembraneⁱ	56 – 76	Helical; Name=1Sequence analysisAdd BLAST	21
Topological domainⁱ	77 – 542	ExtracellularBy similarityAdd BLAST	466
Transmembraneⁱ	543 – 563	Helical; Name=2Sequence analysisAdd BLAST	21
Topological domainⁱ	564 – 650	CytoplasmicBy similarityAdd BLAST	87

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	6	K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-13. 1 Publication		1
Mutagenesisⁱ	13	K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-6. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000181279	1 – 650	Amiloride-sensitive sodium channel subunit gammaAdd BLAST		650

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	210	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	272	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	498	N-linked (GlcNAc...) asparagineSequence analysis	1

Post-translational modificationⁱ

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.1 Publication

Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.1 Publication

ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.By similarity

Keywords - PTMⁱ

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	P37091
PRIDEⁱ	P37091

PTM databases

iPTMnetⁱ	P37091
PhosphoSitePlusⁱ	P37091

Interactionⁱ

Subunit structureⁱ

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (Probable). Interacts with NEDD4; via the WW domains (PubMed:11323714). Interacts with NEDD4L; via the WW domains. Interacts with WWP1; via the WW domains. Interacts with WWP2; via the WW domains. Interacts with the full-length immature form of PCSK9 (pro-PCSK9) (By similarity).By similarity1 Publication1 Publication

GO - Molecular functionⁱ

WW domain binding
Source: RGD
Inferred from physical interactionⁱ
- 8665844

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	246892, 8 interactors
ComplexPortalⁱ	CPX-314 Amiloride-sensitive sodium channel complex, alpha-beta-gamma
Database of interacting proteins More...DIPⁱ	DIP-61327N
ELMⁱ	P37091
IntActⁱ	P37091, 3 interactors
STRINGⁱ	10116.ENSRNOP00000024057

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG4294 Eukaryota ENOG410ZNFK LUCA
HOGENOMⁱ	HOG000236286
HOVERGENⁱ	HBG058435
InParanoidⁱ	P37091
KEGG Orthology (KO) More...KOⁱ	K04827
PhylomeDBⁱ	P37091

Family and domain databases

InterProⁱ	View protein in InterPro IPR001873 ENaC IPR004724 ENaC_chordates IPR020903 ENaC_CS
PANTHERⁱ	PTHR11690 PTHR11690, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00858 ASC, 1 hit
PRINTSⁱ	PR01078 AMINACHANNEL
TIGRFAMsⁱ	TIGR00859 ENaC, 1 hit
PROSITEⁱ	View protein in PROSITE PS01206 ASC, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.ⁱShow all

P37091-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCMNTNTHG CRRIVVSRGR 
        60         70         80         90        100
LRRLLWIAFT LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC 
       110        120        130        140        150
NINPYKYSAV SDLLTDLDSE TKQALLSLYG VKESRKRREA GSMPSTLEGT 
       160        170        180        190        200
PPRFFKLIPL LVFNENEKGK ARDFFTGRKR KISGKIIHKA SNVMHVHESK 
       210        220        230        240        250
KLVGFQLCSN DTSDCATYTF SSGINAIQEW YKLHYMNIMA QVPLEKKINM 
       260        270        280        290        300
SYSAEELLVT CFFDGMSCDA RNFTLFHHPM YGNCYTFNNK ENATILSTSM 
       310        320        330        340        350
GGSEYGLQVI LYINEDEYNP FLVSSTGAKV LIHQQNEYPF IEDVGMEIET 
       360        370        380        390        400
AMSTSIGMHL TESFKLSEPY SQCTEDGSDV PVTNIYNAAY SLQICLYSCF 
       410        420        430        440        450
QTKMVEKCGC AQYSQPLPPA ANYCNYQQHP NWMYCYYQLY QAFVREELGC 
       460        470        480        490        500
QSVCKQSCSF KEWTLTTSLA QWPSEASEKW LLNVLTWDQS QQINKKLNKT 
       510        520        530        540        550
DLAKLLIFYK DLNQRSIMES PANSIEMLLS NFGGQLGLWM SCSVVCVIEI 
       560        570        580        590        600
IEVFFIDFFS IIARRQWHKA KDWWARRQTP PSTETPSSRQ GQDNPALDTD 
       610        620        630        640        650
DDLPTFTSAM RLPPAPGSTV PGTPPPRYNT LRLDRAFSSQ LTDTQLTNEL

Length:650

Mass (Da):74,066

Last modified:November 1, 1997 - v2

Checksum:ⁱ701F9B28B3250D8F

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	G3V8A6	G3V8A6_RAT		Amiloride-sensitive sodium channel ... Amiloride-sensitive sodium channel subunit gamma (Sodium channel, nonvoltage-gated 1 gamma)	Scnn1g rCG_39486	650	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	53	R → P in CAA54905 (PubMed:8107805).Curated		1
Sequence conflictⁱ	573	W → C in CAA54905 (PubMed:8107805).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X77933 mRNA Translation: CAA54905.1 X78034 mRNA Translation: CAA54964.1 U37539 mRNA Translation: AAB58459.1 U37540 mRNA Translation: AAB58460.1
PIRⁱ	A54065
NCBI Reference Sequences More...RefSeqⁱ	NP_058742.1, NM_017046.1
UniGeneⁱ	Rn.10360

Genome annotation databases

GeneIDⁱ	24768
KEGGⁱ	rno:24768
UCSC genome browser More...UCSCⁱ	RGD:3641 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P37091	Amiloride-sensitive sodium channel subunit gamma		MOUSE		655	UniRef90_Q9WU39
amiloride-sensitive sodium channel subunit gamma		MESAU		655
Amiloride-sensitive sodium channel subunit gamma		RAT		650
UPI00022F6600		CRIGR		655
UPI00038C2C84		MICOH		655
+10

Protein	Similar proteins		Species	Score	Length	Source
P37091	Amiloride-sensitive sodium channel subunit gamma		HUMAN		649	UniRef50_P51170
Amiloride-sensitive sodium channel subunit gamma		XENLA		660
Amiloride-sensitive sodium channel subunit gamma		MOUSE		655
Amiloride-sensitive sodium channel subunit gamma		PELSI		669
Amiloride-sensitive sodium channel subunit gamma		BOVIN		652
+251

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X77933 mRNA Translation: CAA54905.1 X78034 mRNA Translation: CAA54964.1 U37539 mRNA Translation: AAB58459.1 U37540 mRNA Translation: AAB58460.1
PIRⁱ	A54065
RefSeqⁱ	NP_058742.1, NM_017046.1
UniGeneⁱ	Rn.10360

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	246892, 8 interactors
ComplexPortalⁱ	CPX-314 Amiloride-sensitive sodium channel complex, alpha-beta-gamma
DIPⁱ	DIP-61327N
ELMⁱ	P37091
IntActⁱ	P37091, 3 interactors
STRINGⁱ	10116.ENSRNOP00000024057

PTM databases

iPTMnetⁱ	P37091
PhosphoSitePlusⁱ	P37091

Proteomic databases

PaxDbⁱ	P37091
PRIDEⁱ	P37091

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	24768
KEGGⁱ	rno:24768
UCSCⁱ	RGD:3641 rat

Organism-specific databases

CTDⁱ	6340
RGDⁱ	3641 Scnn1g

Phylogenomic databases

eggNOGⁱ	KOG4294 Eukaryota ENOG410ZNFK LUCA
HOGENOMⁱ	HOG000236286
HOVERGENⁱ	HBG058435
InParanoidⁱ	P37091
KOⁱ	K04827
PhylomeDBⁱ	P37091

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P37091

PROⁱ

PR:P37091

Family and domain databases

InterProⁱ	View protein in InterPro IPR001873 ENaC IPR004724 ENaC_chordates IPR020903 ENaC_CS
PANTHERⁱ	PTHR11690 PTHR11690, 1 hit
Pfamⁱ	View protein in Pfam PF00858 ASC, 1 hit
PRINTSⁱ	PR01078 AMINACHANNEL
TIGRFAMsⁱ	TIGR00859 ENaC, 1 hit
PROSITEⁱ	View protein in PROSITE PS01206 ASC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SCNNG_RAT
Accessionⁱ	P37091Primary (citable) accession number: P37091
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
	Last sequence update:	November 1, 1997
	Last modified:	September 12, 2018
	This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P37091 (SCNNG_RAT)

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Amiloride-sensitive sodium channel subunit gamma

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Computationally mapped potential isoform sequencesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ