UniProtKB - P37091 (SCNNG_RAT)
Amiloride-sensitive sodium channel subunit gamma
Scnn1g
Functioni
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride (PubMed:9118951, PubMed:8188647).
Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells (PubMed:9118951, PubMed:8188647).
Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus (By similarity).
Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception (By similarity).
By similarity2 PublicationsActivity regulationi
GO - Molecular functioni
- ion channel activity Source: RGD
- ligand-gated sodium channel activity Source: RGD
- WW domain binding Source: RGD
GO - Biological processi
- cellular response to aldosterone Source: RGD
- cellular response to amiloride Source: ComplexPortal
- cellular sodium ion homeostasis Source: ComplexPortal
- multicellular organismal water homeostasis Source: UniProtKB
- response to hypoxia Source: RGD
- sensory perception of taste Source: UniProtKB-KW
- sodium ion homeostasis Source: UniProtKB
- sodium ion import across plasma membrane Source: ComplexPortal
- sodium ion transmembrane transport Source: RGD
- sodium ion transport Source: RGD
- wound healing, spreading of epidermal cells Source: RGD
Keywordsi
Molecular function | Ion channel, Sodium channel |
Biological process | Ion transport, Sensory transduction, Sodium transport, Taste, Transport |
Ligand | Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-2672351, Stimuli-sensing channels R-RNO-9730628, Sensory perception of salty taste |
Names & Taxonomyi
Protein namesi | Recommended name: Amiloride-sensitive sodium channel subunit gammaAlternative name(s): Epithelial Na(+) channel subunit gamma Short name: Gamma-ENaC Gamma-NaCH Nonvoltage-gated sodium channel 1 subunit gamma SCNEG |
Gene namesi | Name:Scnn1g |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3641, Scnn1g |
Subcellular locationi
Plasma membrane
- Apical cell membrane 2 Publications; Multi-pass membrane protein By similarity
Note: Apical membrane of epithelial cells.1 Publication
Extracellular region or secreted
- extracellular exosome Source: RGD
Plasma Membrane
- apical plasma membrane Source: RGD
- external side of plasma membrane Source: RGD
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: RGD
- plasma membrane protein complex Source: RGD
Other locations
- cell surface Source: RGD
- integral component of membrane Source: RGD
- membrane Source: RGD
- sodium channel complex Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 55 | CytoplasmicBy similarityAdd BLAST | 55 | |
Transmembranei | 56 – 76 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 77 – 542 | ExtracellularBy similarityAdd BLAST | 466 | |
Transmembranei | 543 – 563 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 564 – 650 | CytoplasmicBy similarityAdd BLAST | 87 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 6 | K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-13. 1 Publication | 1 | |
Mutagenesisi | 13 | K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-6. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000181279 | 1 – 650 | Amiloride-sensitive sodium channel subunit gammaAdd BLAST | 650 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 210 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 272 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 498 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P37091 |
PTM databases
GlyGeni | P37091, 3 sites |
iPTMneti | P37091 |
PhosphoSitePlusi | P37091 |
Interactioni
Subunit structurei
Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit (PubMed:8188647, PubMed:9118951). An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (Probable).
Interacts with NEDD4; via the WW domains (PubMed:11323714).
Interacts with NEDD4L; via the WW domains.
Interacts with WWP1; via the WW domains.
Interacts with WWP2; via the WW domains.
Interacts with the full-length immature form of PCSK9 (pro-PCSK9) (By similarity).
By similarity1 Publication2 PublicationsGO - Molecular functioni
- WW domain binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 246892, 8 interactors |
ComplexPortali | CPX-314, Amiloride-sensitive sodium channel complex, alpha-beta-gamma |
DIPi | DIP-61327N |
ELMi | P37091 |
IntActi | P37091, 3 interactors |
STRINGi | 10116.ENSRNOP00000024057 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 577 – 628 | DisorderedSequence analysisAdd BLAST | 52 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 578 – 594 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4294, Eukaryota |
InParanoidi | P37091 |
OrthoDBi | 686369at2759 |
PhylomeDBi | P37091 |
Family and domain databases
InterProi | View protein in InterPro IPR001873, ENaC IPR004724, ENaC_chordates IPR020903, ENaC_CS |
PANTHERi | PTHR11690, PTHR11690, 1 hit |
Pfami | View protein in Pfam PF00858, ASC, 1 hit |
PRINTSi | PR01078, AMINACHANNEL |
TIGRFAMsi | TIGR00859, ENaC, 1 hit |
PROSITEi | View protein in PROSITE PS01206, ASC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCMNTNTHG CRRIVVSRGR
60 70 80 90 100
LRRLLWIAFT LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC
110 120 130 140 150
NINPYKYSAV SDLLTDLDSE TKQALLSLYG VKESRKRREA GSMPSTLEGT
160 170 180 190 200
PPRFFKLIPL LVFNENEKGK ARDFFTGRKR KISGKIIHKA SNVMHVHESK
210 220 230 240 250
KLVGFQLCSN DTSDCATYTF SSGINAIQEW YKLHYMNIMA QVPLEKKINM
260 270 280 290 300
SYSAEELLVT CFFDGMSCDA RNFTLFHHPM YGNCYTFNNK ENATILSTSM
310 320 330 340 350
GGSEYGLQVI LYINEDEYNP FLVSSTGAKV LIHQQNEYPF IEDVGMEIET
360 370 380 390 400
AMSTSIGMHL TESFKLSEPY SQCTEDGSDV PVTNIYNAAY SLQICLYSCF
410 420 430 440 450
QTKMVEKCGC AQYSQPLPPA ANYCNYQQHP NWMYCYYQLY QAFVREELGC
460 470 480 490 500
QSVCKQSCSF KEWTLTTSLA QWPSEASEKW LLNVLTWDQS QQINKKLNKT
510 520 530 540 550
DLAKLLIFYK DLNQRSIMES PANSIEMLLS NFGGQLGLWM SCSVVCVIEI
560 570 580 590 600
IEVFFIDFFS IIARRQWHKA KDWWARRQTP PSTETPSSRQ GQDNPALDTD
610 620 630 640 650
DDLPTFTSAM RLPPAPGSTV PGTPPPRYNT LRLDRAFSSQ LTDTQLTNEL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V8A6 | G3V8A6_RAT | Amiloride-sensitive sodium channel ... | Scnn1g rCG_39486 | 650 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 53 | R → P in CAA54905 (PubMed:8107805).Curated | 1 | |
Sequence conflicti | 573 | W → C in CAA54905 (PubMed:8107805).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77933 mRNA Translation: CAA54905.1 X78034 mRNA Translation: CAA54964.1 U37539 mRNA Translation: AAB58459.1 U37540 mRNA Translation: AAB58460.1 |
PIRi | A54065 |
RefSeqi | NP_058742.1, NM_017046.1 |
Genome annotation databases
GeneIDi | 24768 |
KEGGi | rno:24768 |
UCSCi | RGD:3641, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77933 mRNA Translation: CAA54905.1 X78034 mRNA Translation: CAA54964.1 U37539 mRNA Translation: AAB58459.1 U37540 mRNA Translation: AAB58460.1 |
PIRi | A54065 |
RefSeqi | NP_058742.1, NM_017046.1 |
3D structure databases
SMRi | P37091 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246892, 8 interactors |
ComplexPortali | CPX-314, Amiloride-sensitive sodium channel complex, alpha-beta-gamma |
DIPi | DIP-61327N |
ELMi | P37091 |
IntActi | P37091, 3 interactors |
STRINGi | 10116.ENSRNOP00000024057 |
PTM databases
GlyGeni | P37091, 3 sites |
iPTMneti | P37091 |
PhosphoSitePlusi | P37091 |
Proteomic databases
PaxDbi | P37091 |
Genome annotation databases
GeneIDi | 24768 |
KEGGi | rno:24768 |
UCSCi | RGD:3641, rat |
Organism-specific databases
CTDi | 6340 |
RGDi | 3641, Scnn1g |
Phylogenomic databases
eggNOGi | KOG4294, Eukaryota |
InParanoidi | P37091 |
OrthoDBi | 686369at2759 |
PhylomeDBi | P37091 |
Enzyme and pathway databases
Reactomei | R-RNO-2672351, Stimuli-sensing channels R-RNO-9730628, Sensory perception of salty taste |
Miscellaneous databases
PROi | PR:P37091 |
Family and domain databases
InterProi | View protein in InterPro IPR001873, ENaC IPR004724, ENaC_chordates IPR020903, ENaC_CS |
PANTHERi | PTHR11690, PTHR11690, 1 hit |
Pfami | View protein in Pfam PF00858, ASC, 1 hit |
PRINTSi | PR01078, AMINACHANNEL |
TIGRFAMsi | TIGR00859, ENaC, 1 hit |
PROSITEi | View protein in PROSITE PS01206, ASC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SCNNG_RAT | |
Accessioni | P37091Primary (citable) accession number: P37091 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families