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Protein

Amiloride-sensitive sodium channel subunit gamma

Gene

Scnn1g

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.By similarity1 Publication

Activity regulationi

Activated by WNK1, WNK2, WNK3 and WNK4.By similarity

GO - Molecular functioni

  • ligand-gated sodium channel activity Source: RGD
  • WW domain binding Source: RGD

GO - Biological processi

  • multicellular organismal water homeostasis Source: UniProtKB
  • response to hypoxia Source: RGD
  • sensory perception of taste Source: UniProtKB-KW
  • sodium ion homeostasis Source: UniProtKB
  • sodium ion transport Source: RGD
  • wound healing, spreading of epidermal cells Source: RGD

Keywordsi

Molecular functionIon channel, Sodium channel
Biological processIon transport, Sensory transduction, Sodium transport, Taste, Transport
LigandSodium

Names & Taxonomyi

Protein namesi
Recommended name:
Amiloride-sensitive sodium channel subunit gamma
Alternative name(s):
Epithelial Na(+) channel subunit gamma
Short name:
Gamma-ENaC
Gamma-NaCH
Nonvoltage-gated sodium channel 1 subunit gamma
SCNEG
Gene namesi
Name:Scnn1g
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3641 Scnn1g

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55CytoplasmicBy similarityAdd BLAST55
Transmembranei56 – 76Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini77 – 542ExtracellularBy similarityAdd BLAST466
Transmembranei543 – 563Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini564 – 650CytoplasmicBy similarityAdd BLAST87

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-13. 1 Publication1
Mutagenesisi13K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-6. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001812791 – 650Amiloride-sensitive sodium channel subunit gammaAdd BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi210N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi272N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi498N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.1 Publication
Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.1 Publication
ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP37091
PRIDEiP37091

PTM databases

iPTMnetiP37091
PhosphoSitePlusiP37091

Interactioni

Subunit structurei

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (Probable). Interacts with NEDD4; via the WW domains (PubMed:11323714). Interacts with NEDD4L; via the WW domains. Interacts with WWP1; via the WW domains. Interacts with WWP2; via the WW domains. Interacts with the full-length immature form of PCSK9 (pro-PCSK9) (By similarity).By similarity1 Publication1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246892, 8 interactors
ComplexPortaliCPX-314 Amiloride-sensitive sodium channel complex, alpha-beta-gamma
DIPiDIP-61327N
ELMiP37091
IntActiP37091, 3 interactors
STRINGi10116.ENSRNOP00000024057

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4294 Eukaryota
ENOG410ZNFK LUCA
HOGENOMiHOG000236286
HOVERGENiHBG058435
InParanoidiP37091
KOiK04827
PhylomeDBiP37091

Family and domain databases

InterProiView protein in InterPro
IPR001873 ENaC
IPR004724 ENaC_chordates
IPR020903 ENaC_CS
PANTHERiPTHR11690 PTHR11690, 1 hit
PfamiView protein in Pfam
PF00858 ASC, 1 hit
PRINTSiPR01078 AMINACHANNEL
TIGRFAMsiTIGR00859 ENaC, 1 hit
PROSITEiView protein in PROSITE
PS01206 ASC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P37091-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCMNTNTHG CRRIVVSRGR
60 70 80 90 100
LRRLLWIAFT LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC
110 120 130 140 150
NINPYKYSAV SDLLTDLDSE TKQALLSLYG VKESRKRREA GSMPSTLEGT
160 170 180 190 200
PPRFFKLIPL LVFNENEKGK ARDFFTGRKR KISGKIIHKA SNVMHVHESK
210 220 230 240 250
KLVGFQLCSN DTSDCATYTF SSGINAIQEW YKLHYMNIMA QVPLEKKINM
260 270 280 290 300
SYSAEELLVT CFFDGMSCDA RNFTLFHHPM YGNCYTFNNK ENATILSTSM
310 320 330 340 350
GGSEYGLQVI LYINEDEYNP FLVSSTGAKV LIHQQNEYPF IEDVGMEIET
360 370 380 390 400
AMSTSIGMHL TESFKLSEPY SQCTEDGSDV PVTNIYNAAY SLQICLYSCF
410 420 430 440 450
QTKMVEKCGC AQYSQPLPPA ANYCNYQQHP NWMYCYYQLY QAFVREELGC
460 470 480 490 500
QSVCKQSCSF KEWTLTTSLA QWPSEASEKW LLNVLTWDQS QQINKKLNKT
510 520 530 540 550
DLAKLLIFYK DLNQRSIMES PANSIEMLLS NFGGQLGLWM SCSVVCVIEI
560 570 580 590 600
IEVFFIDFFS IIARRQWHKA KDWWARRQTP PSTETPSSRQ GQDNPALDTD
610 620 630 640 650
DDLPTFTSAM RLPPAPGSTV PGTPPPRYNT LRLDRAFSSQ LTDTQLTNEL
Length:650
Mass (Da):74,066
Last modified:November 1, 1997 - v2
Checksum:i701F9B28B3250D8F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V8A6G3V8A6_RAT
Amiloride-sensitive sodium channel ...
Scnn1g rCG_39486
650Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53R → P in CAA54905 (PubMed:8107805).Curated1
Sequence conflicti573W → C in CAA54905 (PubMed:8107805).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77933 mRNA Translation: CAA54905.1
X78034 mRNA Translation: CAA54964.1
U37539 mRNA Translation: AAB58459.1
U37540 mRNA Translation: AAB58460.1
PIRiA54065
RefSeqiNP_058742.1, NM_017046.1
UniGeneiRn.10360

Genome annotation databases

GeneIDi24768
KEGGirno:24768
UCSCiRGD:3641 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77933 mRNA Translation: CAA54905.1
X78034 mRNA Translation: CAA54964.1
U37539 mRNA Translation: AAB58459.1
U37540 mRNA Translation: AAB58460.1
PIRiA54065
RefSeqiNP_058742.1, NM_017046.1
UniGeneiRn.10360

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246892, 8 interactors
ComplexPortaliCPX-314 Amiloride-sensitive sodium channel complex, alpha-beta-gamma
DIPiDIP-61327N
ELMiP37091
IntActiP37091, 3 interactors
STRINGi10116.ENSRNOP00000024057

PTM databases

iPTMnetiP37091
PhosphoSitePlusiP37091

Proteomic databases

PaxDbiP37091
PRIDEiP37091

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24768
KEGGirno:24768
UCSCiRGD:3641 rat

Organism-specific databases

CTDi6340
RGDi3641 Scnn1g

Phylogenomic databases

eggNOGiKOG4294 Eukaryota
ENOG410ZNFK LUCA
HOGENOMiHOG000236286
HOVERGENiHBG058435
InParanoidiP37091
KOiK04827
PhylomeDBiP37091

Miscellaneous databases

PROiPR:P37091

Family and domain databases

InterProiView protein in InterPro
IPR001873 ENaC
IPR004724 ENaC_chordates
IPR020903 ENaC_CS
PANTHERiPTHR11690 PTHR11690, 1 hit
PfamiView protein in Pfam
PF00858 ASC, 1 hit
PRINTSiPR01078 AMINACHANNEL
TIGRFAMsiTIGR00859 ENaC, 1 hit
PROSITEiView protein in PROSITE
PS01206 ASC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSCNNG_RAT
AccessioniPrimary (citable) accession number: P37091
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: September 12, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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