UniProtKB - P37025 (THPR_ECOLI)
Protein
RNA 2',3'-cyclic phosphodiesterase
Gene
thpR
Organism
Escherichia coli (strain K12)
Status
Functioni
Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester (PubMed:25239919). In vitro, can also ligate 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. This reaction does not require ATP and is reversible (PubMed:6347395 and PubMed:8940112).3 Publications
Caution
Was originally thought to be a 2',5'-RNA ligase.2 Publications
Catalytic activityi
- EC:3.1.4.58UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 43 | Proton donorUniRule annotation1 Publication | 1 | |
Active sitei | 125 | Proton acceptorUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity Source: EcoCyc
- 2'-5'-RNA ligase activity Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12330-MONOMER MetaCyc:EG12330-MONOMER |
BRENDAi | 6.5.1.B1, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: RNA 2',3'-cyclic phosphodiesterase1 PublicationUniRule annotation (EC:3.1.4.58UniRule annotation1 Publication)Short name: RNA 2',3'-CPDase1 PublicationUniRule annotation Alternative name(s): Two-histidine 2',3'-cyclic phosphodiesterase acting on RNA1 Publication |
Gene namesi | Name:thpR1 PublicationUniRule annotation Synonyms:ligT, yadP Ordered Locus Names:b0147, JW5011 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Disruption abolishes ligase activity in cell lysates, but mutants grow normally under laboratory conditions.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 43 | H → A: Lack of CPDase activity. 1 Publication | 1 | |
Mutagenesisi | 45 | T → A: Lack of CPDase activity. 1 Publication | 1 | |
Mutagenesisi | 48 | F → A: Decreases CPDase activity. 1 Publication | 1 | |
Mutagenesisi | 125 | H → A: Lack of CPDase activity. 1 Publication | 1 | |
Mutagenesisi | 127 | T → A: Does not affect CPDase activity. 1 Publication | 1 | |
Mutagenesisi | 130 | R → A: Lack of CPDase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000084421 | 2 – 176 | RNA 2',3'-cyclic phosphodiesteraseAdd BLAST | 175 |
Proteomic databases
jPOSTi | P37025 |
PaxDbi | P37025 |
PRIDEi | P37025 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259744, 115 interactors |
STRINGi | 511145.b0147 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37025 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 43 – 46 | HXTX 1UniRule annotationCurated | 4 | |
Motifi | 125 – 128 | HXTX 2UniRule annotationCurated | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1514, Bacteria |
HOGENOMi | CLU_081251_2_1_6 |
InParanoidi | P37025 |
PhylomeDBi | P37025 |
Family and domain databases
HAMAPi | MF_01940, RNA_CPDase, 1 hit |
InterProi | View protein in InterPro IPR009097, Cyclic_Pdiesterase IPR014051, Phosphoesterase_HXTX IPR004175, RNA_CPDase |
PANTHERi | PTHR35561, PTHR35561, 1 hit |
Pfami | View protein in Pfam PF02834, LigT_PEase, 2 hits |
SUPFAMi | SSF55144, SSF55144, 1 hit |
TIGRFAMsi | TIGR02258, 2_5_ligase, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P37025-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEPQRLFFA IDLPAEIREQ IIHWRATHFP PEAGRPVAAD NLHLTLAFLG
60 70 80 90 100
EVSAEKEKAL SLLAGRIRQP GFTLTLDDAG QWLRSRVVWL GMRQPPRGLI
110 120 130 140 150
QLANMLRSQA ARSGCFQSNR PFHPHITLLR DASEAVTIPP PGFNWSYAVT
160 170
EFTLYASSFA RGRTRYTPLK RWALTQ
Sequence cautioni
The sequence M60726 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 174 | L → V in M60726 (PubMed:2013578).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73258.2 AP009048 Genomic DNA Translation: BAB96724.2 M60726 Genomic DNA No translation available. |
PIRi | C64738 |
RefSeqi | NP_414689.4, NC_000913.3 WP_001294700.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73258; AAC73258; b0147 BAB96724; BAB96724; BAB96724 |
GeneIDi | 944848 |
KEGGi | ecj:JW5011 eco:b0147 |
PATRICi | fig|1411691.4.peg.2134 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73258.2 AP009048 Genomic DNA Translation: BAB96724.2 M60726 Genomic DNA No translation available. |
PIRi | C64738 |
RefSeqi | NP_414689.4, NC_000913.3 WP_001294700.1, NZ_STEB01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4QAK | X-ray | 2.02 | A/B | 1-176 | [»] | |
SMRi | P37025 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259744, 115 interactors |
STRINGi | 511145.b0147 |
Proteomic databases
jPOSTi | P37025 |
PaxDbi | P37025 |
PRIDEi | P37025 |
Genome annotation databases
EnsemblBacteriai | AAC73258; AAC73258; b0147 BAB96724; BAB96724; BAB96724 |
GeneIDi | 944848 |
KEGGi | ecj:JW5011 eco:b0147 |
PATRICi | fig|1411691.4.peg.2134 |
Organism-specific databases
EchoBASEi | EB2234 |
Phylogenomic databases
eggNOGi | COG1514, Bacteria |
HOGENOMi | CLU_081251_2_1_6 |
InParanoidi | P37025 |
PhylomeDBi | P37025 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12330-MONOMER MetaCyc:EG12330-MONOMER |
BRENDAi | 6.5.1.B1, 2026 |
Miscellaneous databases
PROi | PR:P37025 |
Family and domain databases
HAMAPi | MF_01940, RNA_CPDase, 1 hit |
InterProi | View protein in InterPro IPR009097, Cyclic_Pdiesterase IPR014051, Phosphoesterase_HXTX IPR004175, RNA_CPDase |
PANTHERi | PTHR35561, PTHR35561, 1 hit |
Pfami | View protein in Pfam PF02834, LigT_PEase, 2 hits |
SUPFAMi | SSF55144, SSF55144, 1 hit |
TIGRFAMsi | TIGR02258, 2_5_ligase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THPR_ECOLI | |
Accessioni | P37025Primary (citable) accession number: P37025 Secondary accession number(s): Q8KJQ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families