UniProtKB - P37005 (LAST_ECOLI)
Protein
Uncharacterized tRNA/rRNA methyltransferase LasT
Gene
lasT
Organism
Escherichia coli (strain K12)
Status
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 113 | S-adenosyl-L-methionine; via amide nitrogenBy similarity | 1 | |
Binding sitei | 133 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- RNA binding Source: InterPro
- tRNA (cytosine-2'-O-)-methyltransferase activity Source: GO_Central
- tRNA (uracil-2'-O-)-methyltransferase activity Source: GO_Central
GO - Biological processi
- tRNA nucleoside ribose methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12309-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:lasT Synonyms:yjtD Ordered Locus Names:b4403, JW4366 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159775 | 1 – 228 | Uncharacterized tRNA/rRNA methyltransferase LasTAdd BLAST | 228 |
Proteomic databases
jPOSTi | P37005 |
PaxDbi | P37005 |
PRIDEi | P37005 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262790, 19 interactors |
DIPi | DIP-10084N |
IntActi | P37005, 9 interactors |
STRINGi | 511145.b4403 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 77 – 79 | S-adenosyl-L-methionine bindingBy similarity | 3 | |
Regioni | 140 – 142 | S-adenosyl-L-methionine bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.Curated
Phylogenomic databases
eggNOGi | COG0565, Bacteria |
HOGENOMi | CLU_056931_3_1_6 |
InParanoidi | P37005 |
PhylomeDBi | P37005 |
Family and domain databases
Gene3Di | 3.40.1280.10, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR004384, RNA_MeTrfase_TrmJ/LasT IPR001537, SpoU_MeTrfase IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR42786, PTHR42786, 1 hit |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit |
PIRSFi | PIRSF004808, LasT, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00050, rRNA_methyl_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P37005-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG
60 70 80 90 100
SGDIIDNIKV FPTLAESLHD VDFTVATTAR SRAKYHYYAT PVELVPLLEE
110 120 130 140 150
KSSWMSHAAL VFGREDSGLT NEELALADVL TGVPMVADYP SLNLGQAVMV
160 170 180 190 200
YCYQLATLIQ QPAKSDATAD QHQLQALRER AMTLLTTLAV ADDIKLVDWL
210 220
QQRLGLLEQR DTAMLHRLLH DIEKNITK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | L → V in L20873 (PubMed:8022271).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97299.1 U00096 Genomic DNA Translation: AAC77356.1 AP009048 Genomic DNA Translation: BAE78392.1 L20873 Genomic DNA No translation available. |
PIRi | S56627 |
RefSeqi | NP_418820.1, NC_000913.3 WP_001223132.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77356; AAC77356; b4403 BAE78392; BAE78392; BAE78392 |
GeneIDi | 948924 |
KEGGi | ecj:JW4366 eco:b4403 |
PATRICi | fig|1411691.4.peg.2282 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97299.1 U00096 Genomic DNA Translation: AAC77356.1 AP009048 Genomic DNA Translation: BAE78392.1 L20873 Genomic DNA No translation available. |
PIRi | S56627 |
RefSeqi | NP_418820.1, NC_000913.3 WP_001223132.1, NZ_LN832404.1 |
3D structure databases
SMRi | P37005 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262790, 19 interactors |
DIPi | DIP-10084N |
IntActi | P37005, 9 interactors |
STRINGi | 511145.b4403 |
Proteomic databases
jPOSTi | P37005 |
PaxDbi | P37005 |
PRIDEi | P37005 |
Genome annotation databases
EnsemblBacteriai | AAC77356; AAC77356; b4403 BAE78392; BAE78392; BAE78392 |
GeneIDi | 948924 |
KEGGi | ecj:JW4366 eco:b4403 |
PATRICi | fig|1411691.4.peg.2282 |
Organism-specific databases
EchoBASEi | EB2215 |
Phylogenomic databases
eggNOGi | COG0565, Bacteria |
HOGENOMi | CLU_056931_3_1_6 |
InParanoidi | P37005 |
PhylomeDBi | P37005 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12309-MONOMER |
Miscellaneous databases
PROi | PR:P37005 |
Family and domain databases
Gene3Di | 3.40.1280.10, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR004384, RNA_MeTrfase_TrmJ/LasT IPR001537, SpoU_MeTrfase IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR42786, PTHR42786, 1 hit |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit |
PIRSFi | PIRSF004808, LasT, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00050, rRNA_methyl_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LAST_ECOLI | |
Accessioni | P37005Primary (citable) accession number: P37005 Secondary accession number(s): Q2M5R4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families