UniProtKB - P36967 (SUCA_DICDI)
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
scsA
Functioni
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.
UniRule annotationCatalytic activityi
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 62 | Coenzyme AUniRule annotation | 1 | |
Binding sitei | 179 | Substrate; shared with subunit betaUniRule annotation | 1 | |
Active sitei | 268 | Tele-phosphohistidine intermediateUniRule annotation | 1 |
GO - Molecular functioni
- nucleotide binding Source: UniProtKB-KW
- succinate-CoA ligase (ADP-forming) activity Source: GO_Central
- succinate-CoA ligase (GDP-forming) activity Source: dictyBase
GO - Biological processi
- succinyl-CoA metabolic process Source: dictyBase
- tricarboxylic acid cycle Source: GO_Central
Keywordsi
Molecular function | Ligase |
Biological process | Tricarboxylic acid cycle |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DDI-71403, Citric acid cycle (TCA cycle) |
UniPathwayi | UPA00223;UER00999 |
Names & Taxonomyi
Protein namesi | Recommended name: Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrialUniRule annotation1 Publication (EC:6.2.1.4UniRule annotation1 Publication, EC:6.2.1.5UniRule annotation)Alternative name(s): Succinyl-CoA synthetase subunit alphaUniRule annotation Short name: SCS-alphaUniRule annotation p36 |
Gene namesi | Name:scsA ORF Names:DDB_G0289325 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0289325, scsA |
Subcellular locationi
Mitochondrion
- Mitochondrion UniRule annotation
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- membrane Source: dictyBase
- succinate-CoA ligase complex (ADP-forming) Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 16 | MitochondrionUniRule annotation1 PublicationAdd BLAST | 16 | |
ChainiPRO_0000033338 | 17 – 315 | Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrialUniRule annotationAdd BLAST | 299 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P36967 |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with an ATP-specific beta subunit, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with a GTP-specific beta subunit forms a GDP-forming succinyl-CoA synthetase (G-SCS).
UniRule annotationProtein-protein interaction databases
STRINGi | 44689.DDB0191520 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 36 – 39 | Coenzyme A bindingUniRule annotation | 4 | |
Regioni | 115 – 117 | Coenzyme A bindingUniRule annotation | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1255, Eukaryota |
HOGENOMi | CLU_052104_0_0_1 |
InParanoidi | P36967 |
OMAi | VIICITE |
PhylomeDBi | P36967 |
Family and domain databases
Gene3Di | 3.40.50.261, 1 hit |
HAMAPi | MF_01988, Succ_CoA_alpha, 1 hit |
InterProi | View protein in InterPro IPR017440, Cit_synth/succinyl-CoA_lig_AS IPR033847, Citrt_syn/SCS-alpha_CS IPR003781, CoA-bd IPR005810, CoA_lig_alpha IPR005811, CoA_ligase IPR036291, NAD(P)-bd_dom_sf IPR016102, Succinyl-CoA_synth-like |
Pfami | View protein in Pfam PF02629, CoA_binding, 1 hit PF00549, Ligase_CoA, 1 hit |
PIRSFi | PIRSF001553, SucCS_alpha, 1 hit |
SMARTi | View protein in SMART SM00881, CoA_binding, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF52210, SSF52210, 1 hit |
TIGRFAMsi | TIGR01019, sucCoAalpha, 1 hit |
PROSITEi | View protein in PROSITE PS01216, SUCCINYL_COA_LIG_1, 1 hit PS00399, SUCCINYL_COA_LIG_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MISAGMIARN FGKRFFSTKP SVLINKHTKV ICQGFTGNQG TFHSKQAIEY
60 70 80 90 100
GTNMVGGVSP GKGGQKHLDL PVFNTVKEAK EATGANATVI YVPPPHAAAA
110 120 130 140 150
IKEAIDAEME LVVCITEGIP QQDMVKVKYL LNKQNKTRLI GPNCPGIIKP
160 170 180 190 200
GECKIGIMPG HIHKPGKIGI VSRSGTLTYE AVAQTTAVGL GQSTCIGIGG
210 220 230 240 250
DPFNGTNFID CLKMFTQDPQ TEGIILIGEI GGEAEEEAAQ WLIDNPTDKP
260 270 280 290 300
VVSFIAGLSA PPGRRMGHAG AIISGGKGDA NSKIEALKAA GVTVTFSPAK
310
LGETILQKMN EKKNK
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 17 | S → D AA sequence (PubMed:8448193).Curated | 1 | |
Sequence conflicti | 27 | H → N in AAA85724 (PubMed:7771809).Curated | 1 | |
Sequence conflicti | 27 | H → N AA sequence (PubMed:8448193).Curated | 1 | |
Sequence conflicti | 32 | C → I AA sequence (PubMed:8448193).Curated | 1 | |
Sequence conflicti | 72 | V → A in AAA85724 (PubMed:7771809).Curated | 1 | |
Sequence conflicti | 72 | V → F AA sequence (PubMed:8448193).Curated | 1 | |
Sequence conflicti | 75 | T → G AA sequence (PubMed:8448193).Curated | 1 | |
Sequence conflicti | 81 | E → D AA sequence (PubMed:8448193).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23408 mRNA Translation: AAA85724.1 Frameshift. AAFI02000139 Genomic DNA Translation: EAL62749.1 |
PIRi | S29826 S65966 |
RefSeqi | XP_636263.1, XM_631171.1 |
Genome annotation databases
EnsemblProtistsi | EAL62749; EAL62749; DDB_G0289325 |
GeneIDi | 8627082 |
KEGGi | ddi:DDB_G0289325 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23408 mRNA Translation: AAA85724.1 Frameshift. AAFI02000139 Genomic DNA Translation: EAL62749.1 |
PIRi | S29826 S65966 |
RefSeqi | XP_636263.1, XM_631171.1 |
3D structure databases
AlphaFoldDBi | P36967 |
SMRi | P36967 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0191520 |
Proteomic databases
PaxDbi | P36967 |
Genome annotation databases
EnsemblProtistsi | EAL62749; EAL62749; DDB_G0289325 |
GeneIDi | 8627082 |
KEGGi | ddi:DDB_G0289325 |
Organism-specific databases
dictyBasei | DDB_G0289325, scsA |
Phylogenomic databases
eggNOGi | KOG1255, Eukaryota |
HOGENOMi | CLU_052104_0_0_1 |
InParanoidi | P36967 |
OMAi | VIICITE |
PhylomeDBi | P36967 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER00999 |
Reactomei | R-DDI-71403, Citric acid cycle (TCA cycle) |
Miscellaneous databases
PROi | PR:P36967 |
Family and domain databases
Gene3Di | 3.40.50.261, 1 hit |
HAMAPi | MF_01988, Succ_CoA_alpha, 1 hit |
InterProi | View protein in InterPro IPR017440, Cit_synth/succinyl-CoA_lig_AS IPR033847, Citrt_syn/SCS-alpha_CS IPR003781, CoA-bd IPR005810, CoA_lig_alpha IPR005811, CoA_ligase IPR036291, NAD(P)-bd_dom_sf IPR016102, Succinyl-CoA_synth-like |
Pfami | View protein in Pfam PF02629, CoA_binding, 1 hit PF00549, Ligase_CoA, 1 hit |
PIRSFi | PIRSF001553, SucCS_alpha, 1 hit |
SMARTi | View protein in SMART SM00881, CoA_binding, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF52210, SSF52210, 1 hit |
TIGRFAMsi | TIGR01019, sucCoAalpha, 1 hit |
PROSITEi | View protein in PROSITE PS01216, SUCCINYL_COA_LIG_1, 1 hit PS00399, SUCCINYL_COA_LIG_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SUCA_DICDI | |
Accessioni | P36967Primary (citable) accession number: P36967 Secondary accession number(s): Q54HN8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | December 4, 2007 | |
Last modified: | May 25, 2022 | |
This is version 155 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families