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Protein

DNA-directed RNA polymerase II subunit RPB9

Gene

POLR2I

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Zinc 1By similarity1
Metal bindingi20Zinc 1By similarity1
Metal bindingi39Zinc 1By similarity1
Metal bindingi42Zinc 1By similarity1
Metal bindingi86Zinc 2By similarity1
Metal bindingi89Zinc 2By similarity1
Metal bindingi114Zinc 2By similarity1
Metal bindingi119Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 42C4-typeSequence analysisAdd BLAST26
Zinc fingeri82 – 124TFIIS-typePROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

  • DNA-directed 5'-3' RNA polymerase activity Source: ProtInc
  • nucleic acid binding Source: InterPro
  • zinc ion binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processTranscription
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB9
Short name:
RNA polymerase II subunit B9
Alternative name(s):
DNA-directed RNA polymerase II subunit I
RNA polymerase II 14.5 kDa subunit
Short name:
RPB14.5
Gene namesi
Name:POLR2I
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105258.8
HGNCiHGNC:9196 POLR2I
MIMi180662 gene
neXtProtiNX_P36954

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000105258
PharmGKBiPA33516

Polymorphism and mutation databases

BioMutaiPOLR2I
DMDMi548702

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214671 – 125DNA-directed RNA polymerase II subunit RPB9Add BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP36954
MaxQBiP36954
PaxDbiP36954
PeptideAtlasiP36954
PRIDEiP36954
ProteomicsDBi55242

PTM databases

iPTMnetiP36954
PhosphoSitePlusiP36954

Miscellaneous databases

PMAP-CutDBiP36954

Expressioni

Gene expression databases

BgeeiENSG00000105258 Expressed in 231 organ(s), highest expression level in testis
CleanExiHS_POLR2I
ExpressionAtlasiP36954 baseline and differential
GenevisibleiP36954 HS

Organism-specific databases

HPAiHPA035632
HPA045156

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.1 Publication

Protein-protein interaction databases

BioGridi111434, 51 interactors
CORUMiP36954
DIPiDIP-32917N
IntActiP36954, 20 interactors
STRINGi9606.ENSP00000221859

Structurei

3D structure databases

ProteinModelPortaliP36954
SMRiP36954
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 42C4-typeSequence analysisAdd BLAST26
Zinc fingeri82 – 124TFIIS-typePROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2691 Eukaryota
COG1594 LUCA
GeneTreeiENSGT00550000075063
HOGENOMiHOG000228134
HOVERGENiHBG009981
InParanoidiP36954
KOiK03017
OMAiETRMILF
OrthoDBiEOG091G0W1S
PhylomeDBiP36954
TreeFamiTF103032

Family and domain databases

CDDicd10508 Zn-ribbon_RPB9, 1 hit
InterProiView protein in InterPro
IPR019761 DNA-dir_RNA_pol-M_15_CS
IPR001529 DNA-dir_RNA_pol_M/15kDasu
IPR012164 Rpa12/Rpb9/Rpc10/TFS
IPR034012 Zn_ribbon_RPB9_C
IPR001222 Znf_TFIIS
PfamiView protein in Pfam
PF02150 RNA_POL_M_15KD, 1 hit
PF01096 TFIIS_C, 1 hit
PIRSFiPIRSF005586 RNApol_RpoM, 1 hit
SMARTiView protein in SMART
SM00661 RPOL9, 1 hit
SM00440 ZnF_C2C2, 1 hit
PROSITEiView protein in PROSITE
PS01030 RNA_POL_M_15KD, 1 hit
PS00466 ZF_TFIIS_1, 1 hit
PS51133 ZF_TFIIS_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P36954-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN
60 70 80 90 100
SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKCG HKEAVFFQSH
110 120
SARAEDAMRL YYVCTAPHCG HRWTE
Length:125
Mass (Da):14,523
Last modified:June 1, 1994 - v1
Checksum:i6520687BB57EE018
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EKS1K7EKS1_HUMAN
DNA-directed RNA polymerase II subu...
POLR2I
88Annotation score:
K7EK49K7EK49_HUMAN
DNA-directed RNA polymerase II subu...
POLR2I
46Annotation score:
K7ENX4K7ENX4_HUMAN
DNA-directed RNA polymerase II subu...
POLR2I
57Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23102 Genomic DNA Translation: CAA80649.1
AK312206 mRNA Translation: BAG35139.1
AD001527 Genomic DNA Translation: AAB51181.1
BC017112 mRNA Translation: AAH17112.1
BC067794 mRNA Translation: AAH67794.1
CCDSiCCDS12487.1
PIRiS41621
RefSeqiNP_006224.1, NM_006233.4
UniGeneiHs.47062

Genome annotation databases

EnsembliENST00000221859; ENSP00000221859; ENSG00000105258
GeneIDi5438
KEGGihsa:5438
UCSCiuc002ode.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23102 Genomic DNA Translation: CAA80649.1
AK312206 mRNA Translation: BAG35139.1
AD001527 Genomic DNA Translation: AAB51181.1
BC017112 mRNA Translation: AAH17112.1
BC067794 mRNA Translation: AAH67794.1
CCDSiCCDS12487.1
PIRiS41621
RefSeqiNP_006224.1, NM_006233.4
UniGeneiHs.47062

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY6electron microscopy7.20I1-125[»]
5IY7electron microscopy8.60I1-125[»]
5IY8electron microscopy7.90I1-125[»]
5IY9electron microscopy6.30I1-125[»]
5IYAelectron microscopy5.40I1-125[»]
5IYBelectron microscopy3.90I1-125[»]
5IYCelectron microscopy3.90I1-125[»]
5IYDelectron microscopy3.90I1-125[»]
ProteinModelPortaliP36954
SMRiP36954
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111434, 51 interactors
CORUMiP36954
DIPiDIP-32917N
IntActiP36954, 20 interactors
STRINGi9606.ENSP00000221859

PTM databases

iPTMnetiP36954
PhosphoSitePlusiP36954

Polymorphism and mutation databases

BioMutaiPOLR2I
DMDMi548702

Proteomic databases

EPDiP36954
MaxQBiP36954
PaxDbiP36954
PeptideAtlasiP36954
PRIDEiP36954
ProteomicsDBi55242

Protocols and materials databases

DNASUi5438
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221859; ENSP00000221859; ENSG00000105258
GeneIDi5438
KEGGihsa:5438
UCSCiuc002ode.4 human

Organism-specific databases

CTDi5438
EuPathDBiHostDB:ENSG00000105258.8
GeneCardsiPOLR2I
HGNCiHGNC:9196 POLR2I
HPAiHPA035632
HPA045156
MIMi180662 gene
neXtProtiNX_P36954
OpenTargetsiENSG00000105258
PharmGKBiPA33516
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2691 Eukaryota
COG1594 LUCA
GeneTreeiENSGT00550000075063
HOGENOMiHOG000228134
HOVERGENiHBG009981
InParanoidiP36954
KOiK03017
OMAiETRMILF
OrthoDBiEOG091G0W1S
PhylomeDBiP36954
TreeFamiTF103032

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiPOLR2I human
GeneWikiiPOLR2I
GenomeRNAii5438
PMAP-CutDBiP36954
PROiPR:P36954
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105258 Expressed in 231 organ(s), highest expression level in testis
CleanExiHS_POLR2I
ExpressionAtlasiP36954 baseline and differential
GenevisibleiP36954 HS

Family and domain databases

CDDicd10508 Zn-ribbon_RPB9, 1 hit
InterProiView protein in InterPro
IPR019761 DNA-dir_RNA_pol-M_15_CS
IPR001529 DNA-dir_RNA_pol_M/15kDasu
IPR012164 Rpa12/Rpb9/Rpc10/TFS
IPR034012 Zn_ribbon_RPB9_C
IPR001222 Znf_TFIIS
PfamiView protein in Pfam
PF02150 RNA_POL_M_15KD, 1 hit
PF01096 TFIIS_C, 1 hit
PIRSFiPIRSF005586 RNApol_RpoM, 1 hit
SMARTiView protein in SMART
SM00661 RPOL9, 1 hit
SM00440 ZnF_C2C2, 1 hit
PROSITEiView protein in PROSITE
PS01030 RNA_POL_M_15KD, 1 hit
PS00466 ZF_TFIIS_1, 1 hit
PS51133 ZF_TFIIS_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPB9_HUMAN
AccessioniPrimary (citable) accession number: P36954
Secondary accession number(s): B2R5J2, Q6NW05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 10, 2018
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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