UniProtKB - P36945 (RBSK_BACSU)
Protein
Ribokinase
Gene
rbsK
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.UniRule annotation
Catalytic activityi
- EC:2.7.1.15UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation
Activity regulationi
Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.UniRule annotation
: D-ribose degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- D-ribose pyranase (rbsD)
- Ribokinase (rbsK)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 139 | SubstrateUniRule annotation | 1 | |
Binding sitei | 183 | ATPUniRule annotation | 1 | |
Metal bindingi | 236 | PotassiumUniRule annotation | 1 | |
Metal bindingi | 238 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 242 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 242 | SubstrateUniRule annotation | 1 | |
Binding sitei | 266 | ATPUniRule annotation | 1 | |
Metal bindingi | 272 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 275 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 277 | Potassium; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 210 – 215 | ATPUniRule annotation | 6 | |
Nucleotide bindingi | 241 – 242 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- ribokinase activity Source: UniProtKB-UniRule
GO - Biological processi
- D-ribose catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
BioCyci | BSUB:BSU35920-MONOMER |
UniPathwayi | UPA00916;UER00889 |
Names & Taxonomyi
Protein namesi | Recommended name: RibokinaseUniRule annotation (EC:2.7.1.15UniRule annotation)Short name: RKUniRule annotation |
Gene namesi | Name:rbsKUniRule annotation Ordered Locus Names:BSU35920 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080096 | 1 – 293 | RibokinaseAdd BLAST | 293 |
Proteomic databases
jPOSTi | P36945 |
PaxDbi | P36945 |
PRIDEi | P36945 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 224308.BSU35920 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 11 – 13 | Substrate bindingUniRule annotation | 3 | |
Regioni | 39 – 43 | Substrate bindingUniRule annotation | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4108RVA Bacteria COG0524 LUCA |
HOGENOMi | HOG000235950 |
InParanoidi | P36945 |
KOi | K00852 |
OMAi | DIVLIQQ |
PhylomeDBi | P36945 |
Family and domain databases
CDDi | cd01174 ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987 Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173 Carboh/pur_kinase_PfkB_CS IPR011877 D_ribokin IPR011611 PfkB_dom IPR002139 Ribo/fructo_kinase IPR029056 Ribokinase-like |
Pfami | View protein in Pfam PF00294 PfkB, 1 hit |
PRINTSi | PR00990 RIBOKINASE |
SUPFAMi | SSF53613 SSF53613, 1 hit |
TIGRFAMsi | TIGR02152 D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584 PFKB_KINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P36945-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRNICVIGSC SMDLVVTSDK RPKAGETVLG TSFQTVPGGK GANQAVAAAR
60 70 80 90 100
LGAQVFMVGK VGDDHYGTAI LNNLKANGVR TDYMEPVTHT ESGTAHIVLA
110 120 130 140 150
EGDNSIVVVK GANDDITPAY ALNALEQIEK VDMVLIQQEI PEETVDEVCK
160 170 180 190 200
YCHSHDIPII LNPAPARPLK QETIDHATYL TPNEHEASIL FPELTISEAL
210 220 230 240 250
ALYPAKLFIT EGKQGVRYSA GSKEVLIPSF PVEPVDTTGA GDTFNAAFAV
260 270 280 290
ALAEGKDIEA ALRFANRAAS LSVCSFGAQG GMPTRNEVEE LLS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 284 – 293 | TRNEVEELLS → DKK in CAA81049 (PubMed:7921236).Curated | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25798 Genomic DNA Translation: CAA81049.1 Z92953 Genomic DNA Translation: CAB07465.1 AL009126 Genomic DNA Translation: CAB15609.1 |
PIRi | D69690 |
RefSeqi | NP_391473.1, NC_000964.3 WP_009968280.1, NZ_JNCM01000034.1 |
Genome annotation databases
EnsemblBacteriai | CAB15609; CAB15609; BSU35920 |
GeneIDi | 936844 |
KEGGi | bsu:BSU35920 |
PATRICi | fig|224308.179.peg.3889 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25798 Genomic DNA Translation: CAA81049.1 Z92953 Genomic DNA Translation: CAB07465.1 AL009126 Genomic DNA Translation: CAB15609.1 |
PIRi | D69690 |
RefSeqi | NP_391473.1, NC_000964.3 WP_009968280.1, NZ_JNCM01000034.1 |
3D structure databases
SMRi | P36945 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU35920 |
Proteomic databases
jPOSTi | P36945 |
PaxDbi | P36945 |
PRIDEi | P36945 |
Genome annotation databases
EnsemblBacteriai | CAB15609; CAB15609; BSU35920 |
GeneIDi | 936844 |
KEGGi | bsu:BSU35920 |
PATRICi | fig|224308.179.peg.3889 |
Phylogenomic databases
eggNOGi | ENOG4108RVA Bacteria COG0524 LUCA |
HOGENOMi | HOG000235950 |
InParanoidi | P36945 |
KOi | K00852 |
OMAi | DIVLIQQ |
PhylomeDBi | P36945 |
Enzyme and pathway databases
UniPathwayi | UPA00916;UER00889 |
BioCyci | BSUB:BSU35920-MONOMER |
Family and domain databases
CDDi | cd01174 ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987 Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173 Carboh/pur_kinase_PfkB_CS IPR011877 D_ribokin IPR011611 PfkB_dom IPR002139 Ribo/fructo_kinase IPR029056 Ribokinase-like |
Pfami | View protein in Pfam PF00294 PfkB, 1 hit |
PRINTSi | PR00990 RIBOKINASE |
SUPFAMi | SSF53613 SSF53613, 1 hit |
TIGRFAMsi | TIGR02152 D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584 PFKB_KINASES_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBSK_BACSU | |
Accessioni | P36945Primary (citable) accession number: P36945 Secondary accession number(s): P96733 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | December 15, 1998 | |
Last modified: | October 16, 2019 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Bacillus subtilis
Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList - PATHWAY comments
Index of metabolic and biosynthesis pathways