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UniProtKB - P36929 (RSMB_ECOLI)

Entry version 178 (02 Jun 2021)
Sequence version 2 (01 Nov 1995)
Previous versions | rss
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Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei277S-adenosyl-L-methionine1 Publication1
Binding sitei303S-adenosyl-L-methionine1 Publication1
Binding sitei322S-adenosyl-L-methionine1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei375NucleophileBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

  • regulation of transcription, DNA-templated Source: InterPro
  • RNA methylation Source: GO_Central
  • rRNA base methylation Source: EcoCyc
Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG12163-MONOMER
MetaCyc:EG12163-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.1.1.176, 2026

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase B (EC:2.1.1.176)
Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rsmB
Synonyms:fmu/fmv, rrmB, sun, yhdB
Ordered Locus Names:b3289, JW3250
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi325C → A: Reduces activity 3-fold. 2 Publications1
Mutagenesisi375C → A: Loss of activity. 2 Publications1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002117921 – 429Ribosomal RNA small subunit methyltransferase BAdd BLAST429

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P36929

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P36929

PRoteomics IDEntifications database

More...PRIDEi		P36929

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4263436, 49 interactors

Database of interacting proteins

More...DIPi		DIP-10946N

Protein interaction database and analysis system

More...IntActi		P36929, 14 interactors

STRING: functional protein association networks

More...STRINGi		511145.b3289

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1429
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P36929

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P36929

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni254 – 260S-adenosyl-L-methionine binding7

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0144, Bacteria
COG0781, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_005316_0_4_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P36929

Database for complete collections of gene phylogenies

More...PhylomeDBi		P36929

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.940.10, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_01856, 16SrRNA_methyltr_B, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR018314, Fmu/NOL1/Nop2p_CS
IPR001678, MeTrfase_RsmB/NOP2
IPR035926, NusB-like_sf
IPR006027, NusB_RsmB_TIM44
IPR023267, RCMT
IPR004573, rRNA_ssu_MeTfrase_B
IPR023541, rRNA_ssu_MeTfrase_B_ent
IPR029063, SAM-dependent_MTases

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01189, Methyltr_RsmB-F, 1 hit
PF01029, NusB, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR02008, RCMTFAMILY

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48013, SSF48013, 1 hit
SSF53335, SSF53335, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00563, rsmB, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS01153, NOL1_NOP2_SUN, 1 hit
PS51686, SAM_MT_RSMB_NOP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P36929-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG 
        60         70         80         90        100
VLRTLSQLDW LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA 
       110        120        130        140        150
ETVEGAIAIK RPQLKGLING VLRQFQRQQE ELLAEFNASD ARYLHPSWLL 
       160        170        180        190        200
KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR THHSRDSWLA LLDEAGMKGF 
       210        220        230        240        250
PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT WLAPQNGEHI 
       260        270        280        290        300
LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK 
       310        320        330        340        350
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA 
       360        370        380        390        400
QLQSEILDAI WPHLKTGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE 
       410        420 
TGTPEQPGKQ NLPGAEEGDG FFYAKLIKK
Length:429
Mass (Da):48,348
Last modified:November 1, 1995 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1F516223C84D80F8
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA58086 differs from that shown. Reason: Frameshift.Curated
The sequence CAA54369 differs from that shown. Reason: Frameshift. Was originally named Fmu and represented the N-terminal part of the RsmB protein.Curated
The sequence CAA54370 differs from that shown. Reason: Frameshift. Was originally named Fmv and represented the C-terminal part of the RsmB protein.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti3K → C AA sequence (PubMed:10026269).Curated1
Sequence conflicti3K → W AA sequence (PubMed:10026269).Curated1
Sequence conflicti105 – 106GA → RR in CAA54369 (PubMed:8432722).Curated2

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X77091 Genomic DNA Translation: CAA54369.1 Frameshift.
X77091 Genomic DNA Translation: CAA54370.1 Frameshift.
Y10307 Genomic DNA Translation: CAA71359.1
U18997 Genomic DNA Translation: AAA58086.1 Frameshift.
U00096 Genomic DNA Translation: AAC76314.1
AP009048 Genomic DNA Translation: BAE78003.1
X52114 Genomic DNA Translation: CAA36358.1

Protein sequence database of the Protein Information Resource

More...PIRi		D65121

NCBI Reference Sequences

More...RefSeqi		NP_417747.1, NC_000913.3
WP_000744778.1, NZ_SSZK01000040.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC76314; AAC76314; b3289
BAE78003; BAE78003; BAE78003

Database of genes from NCBI RefSeq genomes

More...GeneIDi		947789

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW3250
eco:b3289

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.3443

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77091 Genomic DNA Translation: CAA54369.1 Frameshift.
X77091 Genomic DNA Translation: CAA54370.1 Frameshift.
Y10307 Genomic DNA Translation: CAA71359.1
U18997 Genomic DNA Translation: AAA58086.1 Frameshift.
U00096 Genomic DNA Translation: AAC76314.1
AP009048 Genomic DNA Translation: BAE78003.1
X52114 Genomic DNA Translation: CAA36358.1
PIRiD65121
RefSeqiNP_417747.1, NC_000913.3
WP_000744778.1, NZ_SSZK01000040.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4Fmodel-A228-429[»]
1SQFX-ray2.10A1-429[»]
1SQGX-ray1.65A1-429[»]
SMRiP36929
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4263436, 49 interactors
DIPiDIP-10946N
IntActiP36929, 14 interactors
STRINGi511145.b3289

Proteomic databases

jPOSTiP36929
PaxDbiP36929
PRIDEiP36929

Genome annotation databases

EnsemblBacteriaiAAC76314; AAC76314; b3289
BAE78003; BAE78003; BAE78003
GeneIDi947789
KEGGiecj:JW3250
eco:b3289
PATRICifig|1411691.4.peg.3443

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2082

Phylogenomic databases

eggNOGiCOG0144, Bacteria
COG0781, Bacteria
HOGENOMiCLU_005316_0_4_6
InParanoidiP36929
PhylomeDBiP36929

Enzyme and pathway databases

BioCyciEcoCyc:EG12163-MONOMER
MetaCyc:EG12163-MONOMER
BRENDAi2.1.1.176, 2026

Miscellaneous databases

EvolutionaryTraceiP36929

Protein Ontology

More...PROi		PR:P36929

Family and domain databases

Gene3Di1.10.940.10, 1 hit
HAMAPiMF_01856, 16SrRNA_methyltr_B, 1 hit
InterProiView protein in InterPro
IPR018314, Fmu/NOL1/Nop2p_CS
IPR001678, MeTrfase_RsmB/NOP2
IPR035926, NusB-like_sf
IPR006027, NusB_RsmB_TIM44
IPR023267, RCMT
IPR004573, rRNA_ssu_MeTfrase_B
IPR023541, rRNA_ssu_MeTfrase_B_ent
IPR029063, SAM-dependent_MTases
PfamiView protein in Pfam
PF01189, Methyltr_RsmB-F, 1 hit
PF01029, NusB, 1 hit
PRINTSiPR02008, RCMTFAMILY
SUPFAMiSSF48013, SSF48013, 1 hit
SSF53335, SSF53335, 1 hit
TIGRFAMsiTIGR00563, rsmB, 1 hit
PROSITEiView protein in PROSITE
PS01153, NOL1_NOP2_SUN, 1 hit
PS51686, SAM_MT_RSMB_NOP, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRSMB_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P36929
Secondary accession number(s): P23866, Q2M6V3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1995
Last modified: June 2, 2021
This is version 178 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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