UniProtKB - P36929 (RSMB_ECOLI)
Protein
Ribosomal RNA small subunit methyltransferase B
Gene
rsmB
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.1 Publication
Catalytic activityi
- cytidine967 in 16S rRNA + S-adenosyl-L-methionine = 5-methylcytidine967 in 16S rRNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.176
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 277 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 303 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 322 | S-adenosyl-L-methionine1 Publication | 1 | |
Active sitei | 375 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- rRNA (cytosine-C5-)-methyltransferase activity Source: EcoCyc
GO - Biological processi
- regulation of transcription, DNA-templated Source: InterPro
- RNA methylation Source: GO_Central
- rRNA base methylation Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12163-MONOMER MetaCyc:EG12163-MONOMER |
BRENDAi | 2.1.1.176, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase B (EC:2.1.1.176)Alternative name(s): 16S rRNA m5C967 methyltransferase rRNA (cytosine-C(5)-)-methyltransferase RsmB |
Gene namesi | Name:rsmB Synonyms:fmu/fmv, rrmB, sun, yhdB Ordered Locus Names:b3289, JW3250 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 325 | C → A: Reduces activity 3-fold. 2 Publications | 1 | |
Mutagenesisi | 375 | C → A: Loss of activity. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211792 | 1 – 429 | Ribosomal RNA small subunit methyltransferase BAdd BLAST | 429 |
Proteomic databases
jPOSTi | P36929 |
PaxDbi | P36929 |
PRIDEi | P36929 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263436, 49 interactors |
DIPi | DIP-10946N |
IntActi | P36929, 14 interactors |
STRINGi | 511145.b3289 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P36929 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P36929 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 254 – 260 | S-adenosyl-L-methionine binding | 7 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0144, Bacteria COG0781, Bacteria |
HOGENOMi | CLU_005316_0_4_6 |
InParanoidi | P36929 |
PhylomeDBi | P36929 |
Family and domain databases
Gene3Di | 1.10.940.10, 1 hit |
HAMAPi | MF_01856, 16SrRNA_methyltr_B, 1 hit |
InterProi | View protein in InterPro IPR018314, Fmu/NOL1/Nop2p_CS IPR001678, MeTrfase_RsmB/NOP2 IPR035926, NusB-like_sf IPR006027, NusB_RsmB_TIM44 IPR023267, RCMT IPR004573, rRNA_ssu_MeTfrase_B IPR023541, rRNA_ssu_MeTfrase_B_ent IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit PF01029, NusB, 1 hit |
PRINTSi | PR02008, RCMTFAMILY |
SUPFAMi | SSF48013, SSF48013, 1 hit SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00563, rsmB, 1 hit |
PROSITEi | View protein in PROSITE PS01153, NOL1_NOP2_SUN, 1 hit PS51686, SAM_MT_RSMB_NOP, 1 hit |
i Sequence
Sequence statusi: Complete.
P36929-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG
60 70 80 90 100
VLRTLSQLDW LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA
110 120 130 140 150
ETVEGAIAIK RPQLKGLING VLRQFQRQQE ELLAEFNASD ARYLHPSWLL
160 170 180 190 200
KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR THHSRDSWLA LLDEAGMKGF
210 220 230 240 250
PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT WLAPQNGEHI
260 270 280 290 300
LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK
310 320 330 340 350
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA
360 370 380 390 400
QLQSEILDAI WPHLKTGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE
410 420
TGTPEQPGKQ NLPGAEEGDG FFYAKLIKK
Sequence cautioni
The sequence AAA58086 differs from that shown. Reason: Frameshift.Curated
The sequence CAA54369 differs from that shown. Reason: Frameshift. Was originally named Fmu and represented the N-terminal part of the RsmB protein.Curated
The sequence CAA54370 differs from that shown. Reason: Frameshift. Was originally named Fmv and represented the C-terminal part of the RsmB protein.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | K → C AA sequence (PubMed:10026269).Curated | 1 | |
Sequence conflicti | 3 | K → W AA sequence (PubMed:10026269).Curated | 1 | |
Sequence conflicti | 105 – 106 | GA → RR in CAA54369 (PubMed:8432722).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77091 Genomic DNA Translation: CAA54369.1 Frameshift. X77091 Genomic DNA Translation: CAA54370.1 Frameshift. Y10307 Genomic DNA Translation: CAA71359.1 U18997 Genomic DNA Translation: AAA58086.1 Frameshift. U00096 Genomic DNA Translation: AAC76314.1 AP009048 Genomic DNA Translation: BAE78003.1 X52114 Genomic DNA Translation: CAA36358.1 |
PIRi | D65121 |
RefSeqi | NP_417747.1, NC_000913.3 WP_000744778.1, NZ_SSZK01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC76314; AAC76314; b3289 BAE78003; BAE78003; BAE78003 |
GeneIDi | 947789 |
KEGGi | ecj:JW3250 eco:b3289 |
PATRICi | fig|1411691.4.peg.3443 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77091 Genomic DNA Translation: CAA54369.1 Frameshift. X77091 Genomic DNA Translation: CAA54370.1 Frameshift. Y10307 Genomic DNA Translation: CAA71359.1 U18997 Genomic DNA Translation: AAA58086.1 Frameshift. U00096 Genomic DNA Translation: AAC76314.1 AP009048 Genomic DNA Translation: BAE78003.1 X52114 Genomic DNA Translation: CAA36358.1 |
PIRi | D65121 |
RefSeqi | NP_417747.1, NC_000913.3 WP_000744778.1, NZ_SSZK01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1J4F | model | - | A | 228-429 | [»] | |
1SQF | X-ray | 2.10 | A | 1-429 | [»] | |
1SQG | X-ray | 1.65 | A | 1-429 | [»] | |
SMRi | P36929 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263436, 49 interactors |
DIPi | DIP-10946N |
IntActi | P36929, 14 interactors |
STRINGi | 511145.b3289 |
Proteomic databases
jPOSTi | P36929 |
PaxDbi | P36929 |
PRIDEi | P36929 |
Genome annotation databases
EnsemblBacteriai | AAC76314; AAC76314; b3289 BAE78003; BAE78003; BAE78003 |
GeneIDi | 947789 |
KEGGi | ecj:JW3250 eco:b3289 |
PATRICi | fig|1411691.4.peg.3443 |
Organism-specific databases
EchoBASEi | EB2082 |
Phylogenomic databases
eggNOGi | COG0144, Bacteria COG0781, Bacteria |
HOGENOMi | CLU_005316_0_4_6 |
InParanoidi | P36929 |
PhylomeDBi | P36929 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12163-MONOMER MetaCyc:EG12163-MONOMER |
BRENDAi | 2.1.1.176, 2026 |
Miscellaneous databases
EvolutionaryTracei | P36929 |
PROi | PR:P36929 |
Family and domain databases
Gene3Di | 1.10.940.10, 1 hit |
HAMAPi | MF_01856, 16SrRNA_methyltr_B, 1 hit |
InterProi | View protein in InterPro IPR018314, Fmu/NOL1/Nop2p_CS IPR001678, MeTrfase_RsmB/NOP2 IPR035926, NusB-like_sf IPR006027, NusB_RsmB_TIM44 IPR023267, RCMT IPR004573, rRNA_ssu_MeTfrase_B IPR023541, rRNA_ssu_MeTfrase_B_ent IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit PF01029, NusB, 1 hit |
PRINTSi | PR02008, RCMTFAMILY |
SUPFAMi | SSF48013, SSF48013, 1 hit SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00563, rsmB, 1 hit |
PROSITEi | View protein in PROSITE PS01153, NOL1_NOP2_SUN, 1 hit PS51686, SAM_MT_RSMB_NOP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RSMB_ECOLI | |
Accessioni | P36929Primary (citable) accession number: P36929 Secondary accession number(s): P23866, Q2M6V3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families