UniProtKB - P36842 (NIA_LEPMC)
Protein
Nitrate reductase [NADPH]
Gene
NIAD
Organism
Leptosphaeria maculans (Blackleg fungus) (Phoma lingam)
Status
Functioni
Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activityi
- EC:1.7.1.3
Cofactori
Protein has several cofactor binding sites:- FADBy similarityNote: Binds 1 FAD.By similarity
- hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
- Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 170 | MolybdenumBy similarity | 1 | |
Metal bindingi | 571 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 594 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Binding sitei | 776 | FADBy similarity | 1 | |
Binding sitei | 779 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 695 – 698 | FADBy similarity | 4 | |
Nucleotide bindingi | 712 – 716 | FADBy similarity | 5 | |
Nucleotide bindingi | 726 – 728 | FADBy similarity | 3 | |
Nucleotide bindingi | 863 – 872 | NADPBy similarity | 10 |
GO - Molecular functioni
- FAD binding Source: UniProtKB
- heme binding Source: InterPro
- molybdenum ion binding Source: UniProtKB
- molybdopterin cofactor binding Source: InterPro
- nitrate reductase (NADPH) activity Source: UniProtKB-EC
GO - Biological processi
- nitrate assimilation Source: UniProtKB-KW
- nitric oxide biosynthetic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrate assimilation |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NADP |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NIAD |
Organismi | Leptosphaeria maculans (Blackleg fungus) (Phoma lingam) |
Taxonomic identifieri | 5022 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Leptosphaeriaceae › Leptosphaeria › Leptosphaeria maculans species complex |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166045 | 1 – 893 | Nitrate reductase [NADPH]Add BLAST | 893 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 418 | InterchainSequence analysis |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 536 – 611 | Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 641 – 752 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 112 |
Sequence similaritiesi
Belongs to the nitrate reductase family.Curated
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit 3.40.50.80, 1 hit 3.90.420.10, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR014756, Ig_E-set IPR005066, MoCF_OxRdtse_dimer IPR008335, Mopterin_OxRdtase_euk IPR012137, Nitr_rd_NADH IPR001433, OxRdtase_FAD/NAD-bd IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR022407, OxRdtase_Mopterin_BS IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00970, FAD_binding_6, 1 hit PF03404, Mo-co_dimer, 1 hit PF00175, NAD_binding_1, 1 hit PF00174, Oxidored_molyb, 1 hit |
PIRSFi | PIRSF000233, Nitr_rd_NADH, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00363, CYTOCHROMEB5 PR00407, EUMOPTERIN PR00371, FPNCR |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF55856, SSF55856, 1 hit SSF56524, SSF56524, 1 hit SSF63380, SSF63380, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS51384, FAD_FR, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
i Sequence
Sequence statusi: Complete.
P36842-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVTTQQPAV VPLPSSPRLP IESHAGPRST QLPPSPPETV NGDDPKSITS
60 70 80 90 100
TPAEPDFPLP PPANPKPQVL DIDKPTPDAH VPRDPRLIRL TGVHPFNTEP
110 120 130 140 150
PLTDLYNEGF LTSPELFYVR NHGAVPEVQD EECLDWEFSI EGMVANPLKI
160 170 180 190 200
TLRQLLEEYE NVTYPVTLVC AGNRRKEQNV VRKSKGFAWG AAGVSTALFT
210 220 230 240 250
GVVMKDVIER AKPLRKAKYV CMEGADKLPN GYYGTSVKLN WVMDPNRGIM
260 270 280 290 300
LAHKMNGENL SLDHGKPLRA VVPGQIGGRS VKWLKKLIVT AEPSDNWYHI
310 320 330 340 350
YDNRVLPTMV DPDEAAKNPK WWMDERYAIY DLSPNSAIAF PAHEEKVVLA
360 370 380 390 400
SAENSYNVRG YAYSGGGRRI TRCEVSLNKG KNWRLANIDY AEDKYRDFEG
410 420 430 440 450
RELFGARLDM DWRETSFCWC FWNLDIATAE LRDANDILVR AMDEAMCIQP
460 470 480 490 500
RDMYWSVLGM MNNPWYRITI HHEGDVLRFE HPTQPALIPG GWMERVKKAG
510 520 530 540 550
GNLTNGQWGE QIEGQELENT AVEEVKEIKM TKDGVNRIVE LDELKWHESA
560 570 580 590 600
EYPWFVVNDE VYDGTSFLEG HPGGAQSIIS AAGLDASDEF MAIHSETAKA
610 620 630 640 650
MMPAYHIGTL SPTASKQLSL EEPTSKQASS SSLRPTFLDS RTWSKALLSS
660 670 680 690 700
KTKVSWDTRI FRFKLDHASQ TLGLPTGQHL MIRLRDPVTR EAIIRSYTPI
710 720 730 740 750
SQISEQGFCD VLIKIYADAP GREGGKMTKA LDSIPCGHWV DMKGPIGKFE
760 770 780 790 800
YLGKGVCSIN GNERRVRSMK MICGGSGITP IYQVLRAILQ DSADSTHCTV
810 820 830 840 850
LNGNRLEEDI LCREDLDRFA EENGERCTLV HTLTQAAEGW TGRRGRIGEE
860 870 880 890
LLKEFCGTEE DGLVLVCGPE GLERSVKGLL SGMAWRDDDV IFF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U04445 Genomic DNA Translation: AAA50579.1 U18793 Genomic DNA Translation: AAA82740.1 |
PIRi | S46442 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U04445 Genomic DNA Translation: AAA50579.1 U18793 Genomic DNA Translation: AAA82740.1 |
PIRi | S46442 |
3D structure databases
SMRi | P36842 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit 3.40.50.80, 1 hit 3.90.420.10, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR014756, Ig_E-set IPR005066, MoCF_OxRdtse_dimer IPR008335, Mopterin_OxRdtase_euk IPR012137, Nitr_rd_NADH IPR001433, OxRdtase_FAD/NAD-bd IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR022407, OxRdtase_Mopterin_BS IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00970, FAD_binding_6, 1 hit PF03404, Mo-co_dimer, 1 hit PF00175, NAD_binding_1, 1 hit PF00174, Oxidored_molyb, 1 hit |
PIRSFi | PIRSF000233, Nitr_rd_NADH, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00363, CYTOCHROMEB5 PR00407, EUMOPTERIN PR00371, FPNCR |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF55856, SSF55856, 1 hit SSF56524, SSF56524, 1 hit SSF63380, SSF63380, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS51384, FAD_FR, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NIA_LEPMC | |
Accessioni | P36842Primary (citable) accession number: P36842 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | February 1, 1995 | |
Last modified: | August 12, 2020 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |