UniProtKB - P36678 (GSPM_ECOLI)
Protein
Putative type II secretion system protein M
Gene
gspM
Organism
Escherichia coli (strain K12)
Status
Functioni
Inner membrane component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Plays a role in the complex assembly and recruits GspL resulting in a stable complex in the inner membrane. Provides thus a link between the energy-providing GspE protein in the cytoplasm and the rest of the T2SS machinery.By similarity
Miscellaneous
Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.
GO - Biological processi
- protein secretion by the type II secretion system Source: InterPro
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12173-MONOMER MetaCyc:EG12173-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Putative type II secretion system protein MShort name: T2SS protein M Alternative name(s): Putative general secretion pathway protein M Transport protein PshM |
Gene namesi | Name:gspM Synonyms:hopZ, pshM Ordered Locus Names:b3334, JW5704 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane By similarity; Single-pass membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicBy similarityAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 153 | PeriplasmicBy similarityAdd BLAST | 116 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
- type II protein secretion system complex Source: InterPro
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207323 | 1 – 153 | Putative type II secretion system protein MAdd BLAST | 153 |
Proteomic databases
PaxDbi | P36678 |
PRIDEi | P36678 |
Expressioni
Inductioni
Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication
Interactioni
Subunit structurei
Type II secretion system is composed of four main components: the outer membrane complex, the inner membrane complex, the cytoplasmic secretion ATPase and the periplasm-spanning pseudopilus (By similarity). Forms homodimers (By similarity).
Interacts with GspL.
Interacts with GspE and GspF (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 4262464, 145 interactors |
STRINGi | 511145.b3334 |
Family & Domainsi
Sequence similaritiesi
Belongs to the GSP M family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3149, Bacteria ENOG502ZF85, Bacteria |
HOGENOMi | CLU_1710496_0_0_6 |
Family and domain databases
InterProi | View protein in InterPro IPR007690, T2SS_GspM IPR023229, T2SS_M_periplasmic_sf |
Pfami | View protein in Pfam PF04612, T2SSM, 1 hit |
SUPFAMi | SSF103054, SSF103054, 1 hit |
i Sequence
Sequence statusi: Complete.
P36678-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKSWWAEKS TSEKQIVAAL AVLSLGVFCW LGVIKPIDTY IAEHQSHAQK
60 70 80 90 100
IKKDIKWMQD QASTHGLLGH PALTQPIKNI LLEEAKRENL AITLENGPDN
110 120 130 140 150
TLTIHPVTAP LENVSRWLTT AQVTYGIVIE DLQFTLAGNE EITLRHLSFR
EQQ
Sequence cautioni
The sequence AAA58131 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAC36927 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28106 Genomic DNA Translation: AAC36927.1 Different initiation. U18997 Genomic DNA Translation: AAA58131.1 Different initiation. U00096 Genomic DNA Translation: AAC76359.2 AP009048 Genomic DNA Translation: BAE77957.1 |
PIRi | A65127 |
RefSeqi | NP_417793.2, NC_000913.3 WP_001295161.1, NZ_STEB01000038.1 |
Genome annotation databases
EnsemblBacteriai | AAC76359; AAC76359; b3334 BAE77957; BAE77957; BAE77957 |
GeneIDi | 947841 |
KEGGi | ecj:JW5704 eco:b3334 |
PATRICi | fig|1411691.4.peg.3397 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28106 Genomic DNA Translation: AAC36927.1 Different initiation. U18997 Genomic DNA Translation: AAA58131.1 Different initiation. U00096 Genomic DNA Translation: AAC76359.2 AP009048 Genomic DNA Translation: BAE77957.1 |
PIRi | A65127 |
RefSeqi | NP_417793.2, NC_000913.3 WP_001295161.1, NZ_STEB01000038.1 |
3D structure databases
SMRi | P36678 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262464, 145 interactors |
STRINGi | 511145.b3334 |
Proteomic databases
PaxDbi | P36678 |
PRIDEi | P36678 |
Genome annotation databases
EnsemblBacteriai | AAC76359; AAC76359; b3334 BAE77957; BAE77957; BAE77957 |
GeneIDi | 947841 |
KEGGi | ecj:JW5704 eco:b3334 |
PATRICi | fig|1411691.4.peg.3397 |
Organism-specific databases
EchoBASEi | EB2091 |
Phylogenomic databases
eggNOGi | COG3149, Bacteria ENOG502ZF85, Bacteria |
HOGENOMi | CLU_1710496_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12173-MONOMER MetaCyc:EG12173-MONOMER |
Miscellaneous databases
PROi | PR:P36678 |
Family and domain databases
InterProi | View protein in InterPro IPR007690, T2SS_GspM IPR023229, T2SS_M_periplasmic_sf |
Pfami | View protein in Pfam PF04612, T2SSM, 1 hit |
SUPFAMi | SSF103054, SSF103054, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSPM_ECOLI | |
Accessioni | P36678Primary (citable) accession number: P36678 Secondary accession number(s): Q2M6Z9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | December 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families