UniProtKB - P36566 (CMOM_ECOLI)
Protein
tRNA 5-carboxymethoxyuridine methyltransferase
Gene
cmoM
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the methylation of 5-carboxymethoxyuridine (cmo5U) to form 5-methoxycarbonylmethoxyuridine (mcmo5U) at position 34 in tRNAs. Four tRNAs (tRNA(Ala1), tRNA(Ser1), tRNA(Pro3) and tRNA(Thr4)) are fully modified with mcmo5U in stationary-phase E.coli. Also present at low frequency in tRNA(Leu3) and tRNA(Val1).1 Publication
Catalytic activityi
- 5-carboxymethoxyuridine34 in tRNA + S-adenosyl-L-methionine = 5-methoxycarbonylmethoxyuridine34 in tRNA + S-adenosyl-L-homocysteineUniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 26 | S-adenosyl-L-methionineUniRule annotationBy similarity | 1 | |
Binding sitei | 73 | S-adenosyl-L-methionineUniRule annotationBy similarity | 1 | |
Binding sitei | 119 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotationBy similarity | 1 |
GO - Molecular functioni
- tRNA 5-carboxymethoxyuridine methyltransferase activity Source: EcoCyc
GO - Biological processi
- tRNA methylation Source: EcoCyc
- tRNA wobble uridine modification Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12167-MONOMER MetaCyc:EG12167-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA 5-carboxymethoxyuridine methyltransferaseUniRule annotationCurated (EC:2.1.1.-UniRule annotation1 Publication)Alternative name(s): cmo5U methyltransferase1 PublicationUniRule annotation |
Gene namesi | Name:cmoM1 PublicationUniRule annotation Synonyms:smtA1 Publication, ycbD Ordered Locus Names:b0921, JW0904 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 26 | R → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 73 | D → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 124 | W → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 150 | Y → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 209 | R → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 213 | D → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 246 | R → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 247 | Y → A: Decrease in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000071990 | 1 – 261 | tRNA 5-carboxymethoxyuridine methyltransferaseAdd BLAST | 261 |
Proteomic databases
jPOSTi | P36566 |
PaxDbi | P36566 |
PRIDEi | P36566 |
Expressioni
Inductioni
Expression is positively regulated by H-NS.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261464, 139 interactors |
IntActi | P36566, 11 interactors |
STRINGi | 511145.b0921 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 52 – 53 | S-adenosyl-L-methionine bindingUniRule annotationBy similarity | 2 | |
Regioni | 102 – 103 | S-adenosyl-L-methionine bindingUniRule annotationBy similarity | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoM family.UniRule annotationCurated
Phylogenomic databases
eggNOGi | COG2227, Bacteria |
HOGENOMi | CLU_061533_0_1_6 |
InParanoidi | P36566 |
PhylomeDBi | P36566 |
Family and domain databases
HAMAPi | MF_02057, tRNA_methyltr_CmoM, 1 hit |
InterProi | View protein in InterPro IPR033664, Cmo5U_methylTrfase IPR025714, Methyltranfer_dom IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF13847, Methyltransf_31, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
i Sequence
Sequence statusi: Complete.
P36566-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQDRNFDDIA EKFSRNIYGT TKGQLRQAIL WQDLDRVLAE MGPQKLRVLD
60 70 80 90 100
AGGGEGQTAI KMAERGHQVI LCDLSAQMID RAKQAAEAKG VSDNMQFIHC
110 120 130 140 150
AAQDVASHLE TPVDLILFHA VLEWVADPRS VLQTLWSVLR PGGVLSLMFY
160 170 180 190 200
NAHGLLMHNM VAGNFDYVQA GMPKKKKRTL SPDYPRDPAQ VYLWLEEAGW
210 220 230 240 250
QIMGKTGVRV FHDYLREKHQ QRDCYEALLE LETRYCRQEP YITLGRYIHV
260
TARKPQSKDK V
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 180 | L → V in BAA05456 (PubMed:7513784).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26440 Genomic DNA Translation: BAA05456.1 U00096 Genomic DNA Translation: AAC74007.1 AP009048 Genomic DNA Translation: BAA35667.1 |
PIRi | H64831 |
RefSeqi | NP_415441.1, NC_000913.3 WP_001298300.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC74007; AAC74007; b0921 BAA35667; BAA35667; BAA35667 |
GeneIDi | 57728332 945547 |
KEGGi | ecj:JW0904 eco:b0921 |
PATRICi | fig|1411691.4.peg.1355 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26440 Genomic DNA Translation: BAA05456.1 U00096 Genomic DNA Translation: AAC74007.1 AP009048 Genomic DNA Translation: BAA35667.1 |
PIRi | H64831 |
RefSeqi | NP_415441.1, NC_000913.3 WP_001298300.1, NZ_SSZK01000002.1 |
3D structure databases
SMRi | P36566 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261464, 139 interactors |
IntActi | P36566, 11 interactors |
STRINGi | 511145.b0921 |
Proteomic databases
jPOSTi | P36566 |
PaxDbi | P36566 |
PRIDEi | P36566 |
Genome annotation databases
EnsemblBacteriai | AAC74007; AAC74007; b0921 BAA35667; BAA35667; BAA35667 |
GeneIDi | 57728332 945547 |
KEGGi | ecj:JW0904 eco:b0921 |
PATRICi | fig|1411691.4.peg.1355 |
Organism-specific databases
EchoBASEi | EB2086 |
Phylogenomic databases
eggNOGi | COG2227, Bacteria |
HOGENOMi | CLU_061533_0_1_6 |
InParanoidi | P36566 |
PhylomeDBi | P36566 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12167-MONOMER MetaCyc:EG12167-MONOMER |
Miscellaneous databases
PROi | PR:P36566 |
Family and domain databases
HAMAPi | MF_02057, tRNA_methyltr_CmoM, 1 hit |
InterProi | View protein in InterPro IPR033664, Cmo5U_methylTrfase IPR025714, Methyltranfer_dom IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF13847, Methyltransf_31, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CMOM_ECOLI | |
Accessioni | P36566Primary (citable) accession number: P36566 Secondary accession number(s): P77586 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families