UniProtKB - P36562 (COBT_ECOLI)
Protein
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Gene
cobT
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).
Catalytic activityi
- 5,6-dimethylbenzimidazole + nicotinate β-D-ribonucleotide = α-ribazole 5'-phosphate + H+ + nicotinateEC:2.4.2.21
: alpha-ribazole biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT), Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT), Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT), Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT), Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT), Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT), Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT)
- Adenosylcobalamin/alpha-ribazole phosphatase (cobC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole, the pathway alpha-ribazole biosynthesis and in Nucleoside biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 318 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- cobalamin biosynthetic process Source: EcoliWiki
- nucleoside biosynthetic process Source: InterPro
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Cobalamin biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:DMBPPRIBOSYLTRANS-MONOMER ECOL316407:JW1969-MONOMER |
UniPathwayi | UPA00061;UER00516 |
Names & Taxonomyi
Protein namesi | Recommended name: Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC:2.4.2.21)Short name: NN:DBI PRT Alternative name(s): N(1)-alpha-phosphoribosyltransferase |
Gene namesi | Name:cobT Ordered Locus Names:b1991, JW1969 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000167048 | 1 – 359 | Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferaseAdd BLAST | 359 |
Proteomic databases
jPOSTi | P36562 |
PaxDbi | P36562 |
PRIDEi | P36562 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGridi | 4262028, 15 interactors |
IntActi | P36562, 5 interactors |
STRINGi | 511145.b1991 |
Family & Domainsi
Sequence similaritiesi
Belongs to the CobT family.Curated
Phylogenomic databases
eggNOGi | ENOG4105CUP Bacteria COG2038 LUCA |
HOGENOMi | HOG000263499 |
InParanoidi | P36562 |
KOi | K00768 |
PhylomeDBi | P36562 |
Family and domain databases
CDDi | cd02439 DMB-PRT_CobT, 1 hit |
Gene3Di | 1.10.1610.10, 1 hit 3.40.50.10210, 1 hit |
HAMAPi | MF_00230 CobT, 1 hit |
InterProi | View protein in InterPro IPR003200 Nict_dMeBzImd_PRibTrfase-like IPR023195 Nict_dMeBzImd_PRibTrfase_N IPR017846 Nict_dMeBzImd_PRibTrfase_pro IPR036087 Nict_dMeBzImd_PRibTrfase_sf |
Pfami | View protein in Pfam PF02277 DBI_PRT, 1 hit |
SUPFAMi | SSF52733 SSF52733, 1 hit |
TIGRFAMsi | TIGR03160 cobT_DBIPRT, 1 hit |
i Sequence
Sequence statusi: Complete.
P36562-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQILADLLNT IPAIDSTAMS RAQRHIDGLL KPVGSLGKLE VLAIQLAGMP
60 70 80 90 100
GLNGIPHVGK KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC
110 120 130 140 150
VLAEQAGANV HVIDVGIDTA EPIPGLINMR VARGSGNIAS APAMSRRQAE
160 170 180 190 200
KLLLDVICYT QELAKNGVTL FGVGELGMAN TTPAAAIVST ITGRDPEEVV
210 220 230 240 250
GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGVDVLAKVG GFDLVGIAGV
260 270 280 290 300
MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSAEKGARIA
310 320 330 340 350
LSHLGLEPYL NMEMRLGEGS GAALAMPIIE AACAIYNNMG ELAASNIVLP
GNTTSDLNS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 271 | A → L in AAA78908 (PubMed:7592411).Curated | 1 | |
Sequence conflicti | 301 | L → V in AAA78908 (PubMed:7592411).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33333 Genomic DNA Translation: AAA78908.1 U00096 Genomic DNA Translation: AAC75052.1 AP009048 Genomic DNA Translation: BAA15808.1 L25054 Genomic DNA Translation: AAA56876.1 |
PIRi | F64963 |
RefSeqi | NP_416495.1, NC_000913.3 WP_001166160.1, NZ_SSUV01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC75052; AAC75052; b1991 BAA15808; BAA15808; BAA15808 |
GeneIDi | 946517 |
KEGGi | ecj:JW1969 eco:b1991 |
PATRICi | fig|1411691.4.peg.263 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33333 Genomic DNA Translation: AAA78908.1 U00096 Genomic DNA Translation: AAC75052.1 AP009048 Genomic DNA Translation: BAA15808.1 L25054 Genomic DNA Translation: AAA56876.1 |
PIRi | F64963 |
RefSeqi | NP_416495.1, NC_000913.3 WP_001166160.1, NZ_SSUV01000031.1 |
3D structure databases
SMRi | P36562 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 4262028, 15 interactors |
IntActi | P36562, 5 interactors |
STRINGi | 511145.b1991 |
Proteomic databases
jPOSTi | P36562 |
PaxDbi | P36562 |
PRIDEi | P36562 |
Genome annotation databases
EnsemblBacteriai | AAC75052; AAC75052; b1991 BAA15808; BAA15808; BAA15808 |
GeneIDi | 946517 |
KEGGi | ecj:JW1969 eco:b1991 |
PATRICi | fig|1411691.4.peg.263 |
Organism-specific databases
EchoBASEi | EB2071 |
Phylogenomic databases
eggNOGi | ENOG4105CUP Bacteria COG2038 LUCA |
HOGENOMi | HOG000263499 |
InParanoidi | P36562 |
KOi | K00768 |
PhylomeDBi | P36562 |
Enzyme and pathway databases
UniPathwayi | UPA00061;UER00516 |
BioCyci | EcoCyc:DMBPPRIBOSYLTRANS-MONOMER ECOL316407:JW1969-MONOMER |
Miscellaneous databases
PROi | PR:P36562 |
Family and domain databases
CDDi | cd02439 DMB-PRT_CobT, 1 hit |
Gene3Di | 1.10.1610.10, 1 hit 3.40.50.10210, 1 hit |
HAMAPi | MF_00230 CobT, 1 hit |
InterProi | View protein in InterPro IPR003200 Nict_dMeBzImd_PRibTrfase-like IPR023195 Nict_dMeBzImd_PRibTrfase_N IPR017846 Nict_dMeBzImd_PRibTrfase_pro IPR036087 Nict_dMeBzImd_PRibTrfase_sf |
Pfami | View protein in Pfam PF02277 DBI_PRT, 1 hit |
SUPFAMi | SSF52733 SSF52733, 1 hit |
TIGRFAMsi | TIGR03160 cobT_DBIPRT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COBT_ECOLI | |
Accessioni | P36562Primary (citable) accession number: P36562 Secondary accession number(s): P78075 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | November 13, 2019 | |
This is version 156 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PATHWAY comments
Index of metabolic and biosynthesis pathways