UniProtKB - P36553 (HEM6_ECOLI)
Protein
Oxygen-dependent coproporphyrinogen-III oxidase
Gene
hemF
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation2 Publications
Catalytic activityi
- coproporphyrinogen III + 2 H+ + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IXUniRule annotation2 PublicationsEC:1.3.3.3UniRule annotation2 Publications
Cofactori
Mn2+UniRule annotation1 Publication
Activity regulationi
Inhibited by protoporphyrinogen-IX, by metal chelator (EGTA) and by low concentrations of heavy metal ions.2 Publications
Kineticsi
Kcat is 0.17 min(-1) for decarboxylation of coproporphyrinogen-III (at pH 6 and at 37 degrees Celsius).1 Publication
- KM=2.6 µM for coproporphyrinogen-III (at pH 6 at 37 degrees Celsius)2 Publications
- KM=3.1 µM for coproporphyrinogen-III (at pH 7)2 Publications
- Vmax=1.3 µmol/min/mg enzyme (at pH 6 at 37 degrees Celsius)2 Publications
pH dependencei
Optimum pH is between 6 and 7.2 Publications
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Oxygen-dependent coproporphyrinogen-III oxidase (hemF), Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 92 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 96 | ManganeseCurated | 1 | |
Active sitei | 106 | Proton donorUniRule annotation | 1 | |
Metal bindingi | 106 | ManganeseCurated | 1 | |
Metal bindingi | 145 | ManganeseCurated | 1 | |
Metal bindingi | 175 | ManganeseCurated | 1 | |
Sitei | 175 | Important for dimerizationUniRule annotation | 1 |
GO - Molecular functioni
- coproporphyrinogen oxidase activity Source: EcoCyc
- manganese ion binding Source: EcoCyc
- protein homodimerization activity Source: UniProtKB
- tetrapyrrole binding Source: EcoliWiki
GO - Biological processi
- heme biosynthetic process Source: EcoliWiki
- protoporphyrinogen IX biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Heme biosynthesis, Porphyrin biosynthesis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:COPROGENOXI-MONOMER MetaCyc:COPROGENOXI-MONOMER |
BRENDAi | 1.3.3.3, 2026 |
UniPathwayi | UPA00251;UER00322 |
Names & Taxonomyi
Protein namesi | Recommended name: Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)Short name: CPOUniRule annotation Short name: Coprogen oxidaseUniRule annotation Short name: CoproporphyrinogenaseUniRule annotation |
Gene namesi | Name:hemFUniRule annotation Ordered Locus Names:b2436, JW2429 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: EcoliWiki
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 96 | H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication | 1 | |
Mutagenesisi | 106 | H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication | 1 | |
Mutagenesisi | 145 | H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication | 1 | |
Mutagenesisi | 175 | H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication | 1 | |
Mutagenesisi | 274 | W → L: Complete loss of oxidase activity. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109894 | 1 – 299 | Oxygen-dependent coproporphyrinogen-III oxidaseAdd BLAST | 299 |
Proteomic databases
PaxDbi | P36553 |
PRIDEi | P36553 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 4260574, 13 interactors |
IntActi | P36553, 3 interactors |
STRINGi | 511145.b2436 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 108 – 110 | Substrate bindingUniRule annotation | 3 | |
Regioni | 240 – 275 | Important for dimerizationUniRule annotationAdd BLAST | 36 | |
Regioni | 258 – 260 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0408, Bacteria |
HOGENOMi | CLU_026169_0_1_6 |
InParanoidi | P36553 |
PhylomeDBi | P36553 |
Family and domain databases
Gene3Di | 3.40.1500.10, 1 hit |
HAMAPi | MF_00333, Coprogen_oxidas, 1 hit |
InterProi | View protein in InterPro IPR001260, Coprogen_oxidase_aer IPR036406, Coprogen_oxidase_aer_sf IPR018375, Coprogen_oxidase_CS |
PANTHERi | PTHR10755, PTHR10755, 1 hit |
Pfami | View protein in Pfam PF01218, Coprogen_oxidas, 1 hit |
PIRSFi | PIRSF000166, Coproporphyri_ox, 1 hit |
PRINTSi | PR00073, COPRGNOXDASE |
SUPFAMi | SSF102886, SSF102886, 1 hit |
PROSITEi | View protein in PROSITE PS01021, COPROGEN_OXIDASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P36553-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR
60 70 80 90 100
NGGVFEQAGV NFSHVHGEAM PASATAHRPE LAGRSFEAMG VSLVVHPHNP
110 120 130 140 150
YVPTSHANVR FFIAEKPGAD PVWWFGGGFD LTPFYGFEED AIHWHRTARD
160 170 180 190 200
LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR GIGGLFFDDL NTPDFDRCFA
210 220 230 240 250
FMQAVGKGYT DAYLPIVERR KAMAYGERER NFQLYRRGRY VEFNLVWDRG
260 270 280 290
TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALS EFIKVRDWV
Mass spectrometryi
Molecular mass is 35390 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75413 Genomic DNA Translation: CAA53167.1 U00096 Genomic DNA Translation: AAC75489.1 AP009048 Genomic DNA Translation: BAA16319.1 |
PIRi | B36964 |
RefSeqi | NP_416931.1, NC_000913.3 WP_000801365.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75489; AAC75489; b2436 BAA16319; BAA16319; BAA16319 |
GeneIDi | 946908 |
KEGGi | ecj:JW2429 eco:b2436 |
PATRICi | fig|1411691.4.peg.4295 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75413 Genomic DNA Translation: CAA53167.1 U00096 Genomic DNA Translation: AAC75489.1 AP009048 Genomic DNA Translation: BAA16319.1 |
PIRi | B36964 |
RefSeqi | NP_416931.1, NC_000913.3 WP_000801365.1, NZ_LN832404.1 |
3D structure databases
SMRi | P36553 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260574, 13 interactors |
IntActi | P36553, 3 interactors |
STRINGi | 511145.b2436 |
Proteomic databases
PaxDbi | P36553 |
PRIDEi | P36553 |
Protocols and materials databases
DNASUi | 946908 |
Genome annotation databases
EnsemblBacteriai | AAC75489; AAC75489; b2436 BAA16319; BAA16319; BAA16319 |
GeneIDi | 946908 |
KEGGi | ecj:JW2429 eco:b2436 |
PATRICi | fig|1411691.4.peg.4295 |
Organism-specific databases
EchoBASEi | EB2106 |
Phylogenomic databases
eggNOGi | COG0408, Bacteria |
HOGENOMi | CLU_026169_0_1_6 |
InParanoidi | P36553 |
PhylomeDBi | P36553 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00322 |
BioCyci | EcoCyc:COPROGENOXI-MONOMER MetaCyc:COPROGENOXI-MONOMER |
BRENDAi | 1.3.3.3, 2026 |
Miscellaneous databases
PROi | PR:P36553 |
Family and domain databases
Gene3Di | 3.40.1500.10, 1 hit |
HAMAPi | MF_00333, Coprogen_oxidas, 1 hit |
InterProi | View protein in InterPro IPR001260, Coprogen_oxidase_aer IPR036406, Coprogen_oxidase_aer_sf IPR018375, Coprogen_oxidase_CS |
PANTHERi | PTHR10755, PTHR10755, 1 hit |
Pfami | View protein in Pfam PF01218, Coprogen_oxidas, 1 hit |
PIRSFi | PIRSF000166, Coproporphyri_ox, 1 hit |
PRINTSi | PR00073, COPRGNOXDASE |
SUPFAMi | SSF102886, SSF102886, 1 hit |
PROSITEi | View protein in PROSITE PS01021, COPROGEN_OXIDASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HEM6_ECOLI | |
Accessioni | P36553Primary (citable) accession number: P36553 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families