UniProtKB - P36506 (MP2K2_RAT)
Protein
Dual specificity mitogen-activated protein kinase kinase 2
Gene
Map2k2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (By similarity).By similarity
Catalytic activityi
Cofactori
Mg2+Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 101 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 194 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 78 – 86 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: RGD
- MAP kinase kinase activity Source: RGD
- MAP-kinase scaffold activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- molecular adaptor activity Source: RGD
- PDZ domain binding Source: RGD
- protein serine/threonine/tyrosine kinase activity Source: UniProtKB
- protein serine/threonine kinase activator activity Source: RGD
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein tyrosine kinase activity Source: UniProtKB-KW
- scaffold protein binding Source: RGD
GO - Biological processi
- activation of MAPK activity Source: GO_Central
- epithelial cell proliferation involved in lung morphogenesis Source: RGD
- ERK1 and ERK2 cascade Source: RGD
- face development Source: RGD
- heart development Source: RGD
- lung morphogenesis Source: RGD
- negative regulation of gene expression Source: BHF-UCL
- peptidyl-serine autophosphorylation Source: RGD
- positive regulation of axonogenesis Source: RGD
- positive regulation of cell motility Source: RGD
- positive regulation of production of miRNAs involved in gene silencing by miRNA Source: RGD
- positive regulation of protein serine/threonine kinase activity Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- protein phosphorylation Source: RGD
- regulation of axon regeneration Source: RGD
- regulation of early endosome to late endosome transport Source: UniProtKB
- regulation of Golgi inheritance Source: UniProtKB
- regulation of stress-activated MAPK cascade Source: UniProtKB
- thymus development Source: RGD
- thyroid gland development Source: RGD
- trachea formation Source: RGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.12.2, 5301 |
Reactomei | R-RNO-112411, MAPK1 (ERK2) activation R-RNO-170968, Frs2-mediated activation R-RNO-445144, Signal transduction by L1 R-RNO-5673000, RAF activation R-RNO-5674135, MAP2K and MAPK activation R-RNO-5674499, Negative feedback regulation of MAPK pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Dual specificity mitogen-activated protein kinase kinase 2 (EC:2.7.12.2By similarity)Short name: MAP kinase kinase 2 Short name: MAPKK 2 Alternative name(s): ERK activator kinase 2 MAPK/ERK kinase 2 Short name: MEK 2 |
Gene namesi | Name:Map2k2 Synonyms:Mek2, Mkk2, Prkmk2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61888, Map2k2 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- Membrane By similarity; Peripheral membrane protein By similarity
Note: Membrane localization is probably regulated by its interaction with KSR1.By similarity
Cytoskeleton
- microtubule Source: RGD
Cytosol
- cytosol Source: RGD
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Endosome
- early endosome Source: UniProtKB
- late endosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: RGD
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- cytoplasmic side of plasma membrane Source: RGD
Other locations
- cell cortex Source: RGD
- cell-cell junction Source: RGD
- cytoplasm Source: RGD
- focal adhesion Source: UniProtKB
- perinuclear region of cytoplasm Source: RGD
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086374 | 1 – 400 | Dual specificity mitogen-activated protein kinase kinase 2Add BLAST | 400 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Modified residuei | 222 | Phosphoserine; by RAFBy similarity | 1 | |
Modified residuei | 226 | Phosphoserine; by RAFBy similarity | 1 | |
Modified residuei | 293 | PhosphoserineBy similarity | 1 | |
Modified residuei | 295 | PhosphoserineBy similarity | 1 | |
Modified residuei | 306 | PhosphoserineBy similarity | 1 | |
Modified residuei | 394 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 396 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 10 – 11 | Cleavage; by anthrax lethal factorBy similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P36506 |
PaxDbi | P36506 |
PRIDEi | P36506 |
PTM databases
iPTMneti | P36506 |
PhosphoSitePlusi | P36506 |
Expressioni
Tissue specificityi
Expressed abundantly in the adult brain and muscle.
Gene expression databases
Bgeei | ENSRNOG00000020005, Expressed in jejunum and 21 other tissues |
Genevisiblei | P36506, RN |
Interactioni
Subunit structurei
Interacts with MORG1 (By similarity).
Interacts with SGK1 (By similarity).
Interacts with KSR1.
Interacts with KSR1 and BRAF; the interaction with KSR1 mediates KSR1-BRAF dimerization.
Interacts with GLS (By similarity).
By similarityGO - Molecular functioni
- MAP-kinase scaffold activity Source: RGD
- PDZ domain binding Source: RGD
- scaffold protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248693, 7 interactors |
IntActi | P36506, 1 interactor |
STRINGi | 10116.ENSRNOP00000027272 |
Chemistry databases
BindingDBi | P36506 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 72 – 369 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 298 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 266 – 315 | Pro-richAdd BLAST | 50 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000153487 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | P36506 |
OMAi | CHDMYER |
OrthoDBi | 688282at2759 |
PhylomeDBi | P36506 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P36506-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ
60 70 80 90 100
RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR
110 120 130 140 150
KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM
160 170 180 190 200
DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI
210 220 230 240 250
LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI
260 270 280 290 300
WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP
310 320 330 340 350
PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL
360 370 380 390 400
IKNPAERADL KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14592 mRNA Translation: BAA03442.1 L14936 mRNA Translation: AAA41620.1 BC126084 mRNA Translation: AAI26085.1 |
PIRi | A48081 |
RefSeqi | NP_579817.1, NM_133283.1 |
Genome annotation databases
Ensembli | ENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005 |
GeneIDi | 58960 |
KEGGi | rno:58960 |
UCSCi | RGD:61888, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14592 mRNA Translation: BAA03442.1 L14936 mRNA Translation: AAA41620.1 BC126084 mRNA Translation: AAI26085.1 |
PIRi | A48081 |
RefSeqi | NP_579817.1, NM_133283.1 |
3D structure databases
SMRi | P36506 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248693, 7 interactors |
IntActi | P36506, 1 interactor |
STRINGi | 10116.ENSRNOP00000027272 |
Chemistry databases
BindingDBi | P36506 |
ChEMBLi | CHEMBL3430876 |
PTM databases
iPTMneti | P36506 |
PhosphoSitePlusi | P36506 |
Proteomic databases
jPOSTi | P36506 |
PaxDbi | P36506 |
PRIDEi | P36506 |
Genome annotation databases
Ensembli | ENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005 |
GeneIDi | 58960 |
KEGGi | rno:58960 |
UCSCi | RGD:61888, rat |
Organism-specific databases
CTDi | 5605 |
RGDi | 61888, Map2k2 |
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000153487 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | P36506 |
OMAi | CHDMYER |
OrthoDBi | 688282at2759 |
PhylomeDBi | P36506 |
Enzyme and pathway databases
BRENDAi | 2.7.12.2, 5301 |
Reactomei | R-RNO-112411, MAPK1 (ERK2) activation R-RNO-170968, Frs2-mediated activation R-RNO-445144, Signal transduction by L1 R-RNO-5673000, RAF activation R-RNO-5674135, MAP2K and MAPK activation R-RNO-5674499, Negative feedback regulation of MAPK pathway |
Miscellaneous databases
PROi | PR:P36506 |
Gene expression databases
Bgeei | ENSRNOG00000020005, Expressed in jejunum and 21 other tissues |
Genevisiblei | P36506, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MP2K2_RAT | |
Accessioni | P36506Primary (citable) accession number: P36506 Secondary accession number(s): A0JN15 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families