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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei579ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU30320-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BSU30320
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001519731 – 804Leucine--tRNA ligaseAdd BLAST804

Proteomic databases

PaxDbiP36430
PRIDEiP36430

Interactioni

Protein-protein interaction databases

IntActiP36430, 1 interactor
MINTiP36430
STRINGi224308.Bsubs1_010100016511

Structurei

3D structure databases

ProteinModelPortaliP36430
SMRiP36430
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 51"HIGH" regionAdd BLAST12
Motifi576 – 580"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T Bacteria
COG0495 LUCA
HOGENOMiHOG000200748
InParanoidiP36430
KOiK01869
OMAiTFMVLAP
PhylomeDBiP36430

Family and domain databases

Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_00049_B Leu_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR002302 Leu-tRNA-ligase
IPR025709 Leu_tRNA-synth_edit
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740 PTHR43740, 2 hits
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF13603 tRNA-synt_1_2, 1 hit
PF09334 tRNA-synt_1g, 1 hit
PRINTSiPR00985 TRNASYNTHLEU
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00396 leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P36430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFQHKEIEK KWQTYWLENK TFATLDNNEK QKFYALDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI LSRMKRMQGY DVLHPMGWDA FGLPAEQYAL DTGNDPAVFT
110 120 130 140 150
KQNIDNFRRQ IQALGFSYDW DREINTTDPE YYKWTQWIFL KLYEKGLAYV
160 170 180 190 200
DEVPVNWCPA LGTVLANEEV IDGKSERGGH PVERRPMKQW MLKITAYADR
210 220 230 240 250
LLEDLEELDW PESIKDMQRN WIGRSEGAHV HFAIDGHDDS FTVFTTRPDT
260 270 280 290 300
LFGATYTVLA PEHALVENIT TAEQKEAVEA YIKEIQSKSD LERTDLAKTK
310 320 330 340 350
TGVFTGAYAI NPVNGEKLPI WIADYVLASY GTGAVMAVPG HDERDFEFAK
360 370 380 390 400
TFGLPVKEVV KGGNVEEAAY TGDGEHVNSD FLNGLHKQEA IEKVIAWLEE
410 420 430 440 450
TKNGEKKVTY RLRDWLFSRQ RYWGEPIPVI HWEDGTSTAV PEEELPLILP
460 470 480 490 500
KTDEIKPSGT GESPLANIKE WVEVTDPETG KKGRRETNTM PQWAGSCWYF
510 520 530 540 550
LRYIDPHNPD QLASPEKLEK WLPVDMYIGG AEHAVLHLLY ARFWHKFLYD
560 570 580 590 600
IGVVPTKEPF QKLYNQGMIL GENNEKMSKS KGNVVNPDEI VASHGADTLR
610 620 630 640 650
LYEMFMGPLD ASIAWSESGL DGARRFLDRV WRLFIEDSGE LNGKIVEGAG
660 670 680 690 700
ETLERVYHET VMKVTDHYEG LRFNTGISQL MVFINEAYKA TELPKEYMEG
710 720 730 740 750
FVKLLSPVAP HLAEELWEKL GHSGTIAYEA WPVYDETKLV DDEVEIVVQL
760 770 780 790 800
NGKVKAKLQV PADATKEQLE QLAQADEKVK EQLEGKTIRK IIAVPGKLVN

IVAN
Length:804
Mass (Da):91,543
Last modified:July 15, 1998 - v3
Checksum:i306FD5A98FE5C47E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186P → L in AAA22571 (PubMed:1317842).Curated1
Sequence conflicti195T → N in AAA22571 (PubMed:1317842).Curated1
Sequence conflicti247 – 281RPDTL…AVEAY → DQIRCLALHTLSLPRNTHWW KTSQRQSKKKLLKLI (PubMed:1317842).CuratedAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88581 Genomic DNA Translation: AAA22571.1
AF008220 Genomic DNA Translation: AAC00259.1
AL009126 Genomic DNA Translation: CAB15010.1
PIRiD69650
RefSeqiNP_390910.1, NC_000964.3
WP_003246072.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB15010; CAB15010; BSU30320
GeneIDi938102
KEGGibsu:BSU30320
PATRICifig|224308.179.peg.3288

Similar proteinsi

Entry informationi

Entry nameiSYL_BACSU
AccessioniPrimary (citable) accession number: P36430
Secondary accession number(s): O34465
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 15, 1998
Last modified: February 28, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. SIMILARITY comments
    Index of protein domains and families

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