UniProtKB - P36406 (TRI23_HUMAN)
E3 ubiquitin-protein ligase TRIM23
TRIM23
Functioni
Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes (PubMed:28871090).
2 Publications(Microbial infection) Mediates TRAF6 auto-ubiquitination in the presence of human cytomegalovirus protein UL144, resulting in the virally controlled activation of NF-kappa-B stimulation at early times of HCMV infection.
1 PublicationCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 31 – 76 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 46 | |
Zinc fingeri | 122 – 168 | B box-type; degeneratePROSITE-ProRule annotationAdd BLAST | 47 | |
Nucleotide bindingi | 411 – 418 | GTPBy similarity | 8 | |
Nucleotide bindingi | 454 – 458 | GTPBy similarity | 5 | |
Nucleotide bindingi | 513 – 516 | GTPBy similarity | 4 |
GO - Molecular functioni
- enzyme activator activity Source: ProtInc
- GDP binding Source: HGNC-UCL
- GTPase activity Source: HGNC-UCL
- GTP binding Source: HGNC-UCL
- identical protein binding Source: IntAct
- ubiquitin-protein transferase activity Source: HGNC-UCL
- zinc ion binding Source: InterPro
GO - Biological processi
- innate immune response Source: UniProtKB-KW
- intracellular protein transport Source: GO_Central
- protein ubiquitination Source: HGNC-UCL
- vesicle-mediated transport Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Host-virus interaction, Immunity, Innate immunity, Ubl conjugation pathway |
Ligand | GTP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 2.3.2.27, 2681 |
PathwayCommonsi | P36406 |
SignaLinki | P36406 |
SIGNORi | P36406 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase TRIM23 (EC:2.3.2.27)Alternative name(s): ADP-ribosylation factor domain-containing protein 1 GTP-binding protein ARD-1 RING finger protein 46 RING-type E3 ubiquitin transferase TRIM23Curated Tripartite motif-containing protein 23 |
Gene namesi | Name:TRIM23 Synonyms:ARD1, ARFD1, RNF46 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:660, TRIM23 |
MIMi | 601747, gene |
neXtProti | NX_P36406 |
VEuPathDBi | HostDB:ENSG00000113595 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 1 Publication
Lysosome
- Lysosome membrane 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Endomembrane system 1 Publication
Note: Membrane-associated with the Golgi complex and lysosomal structures.
Golgi apparatus
- Golgi membrane Source: HGNC-UCL
Lysosome
- lysosomal membrane Source: HGNC-UCL
Nucleus
- nucleus Source: Ensembl
Plasma Membrane
- plasma membrane Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Golgi apparatus, Lysosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 34 | C → A: Loss of E3 ubiquitin-protein ligase activity. 1 Publication | 1 | |
Mutagenesisi | 53 | H → A: Loss of E3 ubiquitin-protein ligase activity. 1 Publication | 1 | |
Mutagenesisi | 418 | T → N: Maintains GTPase activity. Increases interaction with PSCD1. 1 Publication | 1 | |
Mutagenesisi | 458 | K → I: Suppresses GTPase activity. Decreases interaction with PSCD1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 373 |
OpenTargetsi | ENSG00000113595 |
PharmGKBi | PA24943 |
Miscellaneous databases
Pharosi | P36406, Tbio |
Genetic variation databases
BioMutai | TRIM23 |
DMDMi | 543839 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207483 | 1 – 574 | E3 ubiquitin-protein ligase TRIM23Add BLAST | 574 |
Proteomic databases
EPDi | P36406 |
jPOSTi | P36406 |
MassIVEi | P36406 |
MaxQBi | P36406 |
PaxDbi | P36406 |
PeptideAtlasi | P36406 |
PRIDEi | P36406 |
ProteomicsDBi | 55200 [P36406-1] 55201 [P36406-2] 55202 [P36406-3] |
PTM databases
iPTMneti | P36406 |
PhosphoSitePlusi | P36406 |
Expressioni
Gene expression databases
Bgeei | ENSG00000113595, Expressed in corpus callosum and 226 other tissues |
ExpressionAtlasi | P36406, baseline and differential |
Genevisiblei | P36406, HS |
Organism-specific databases
HPAi | ENSG00000113595, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
Interacts with PSCD1.
Interacts with UBE2D2 (PubMed:28681414).
Interacts with TBK1 (via N-terminal kinase domain) and p62/SQSTM1.
3 Publications(Microbial infection) Interacts with human cytomegalovirus protein UL144; this interaction might cause autoubiquitination of TRAF6, leading to NF-kappa-B activation.
1 PublicationBinary interactionsi
P36406
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 106868, 243 interactors |
CORUMi | P36406 |
IntActi | P36406, 232 interactors |
MINTi | P36406 |
STRINGi | 9606.ENSP00000231524 |
Miscellaneous databases
RNActi | P36406, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P36406 |
SMRi | P36406 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 390 – 574 | ARF-likeAdd BLAST | 185 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 352 – 379 | Sequence analysisAdd BLAST | 28 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 31 – 76 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 46 | |
Zinc fingeri | 122 – 168 | B box-type; degeneratePROSITE-ProRule annotationAdd BLAST | 47 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0070, Eukaryota KOG4185, Eukaryota |
GeneTreei | ENSGT00940000158562 |
HOGENOMi | CLU_033905_0_0_1 |
InParanoidi | P36406 |
OMAi | WHIQGSS |
OrthoDBi | 1362554at2759 |
PhylomeDBi | P36406 |
TreeFami | TF320703 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003649, Bbox_C IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR024156, Small_GTPase_ARF IPR006689, Small_GTPase_ARF/SAR IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR013087, Znf_C2H2_type IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR11711, PTHR11711, 1 hit |
Pfami | View protein in Pfam PF00025, Arf, 1 hit PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SMARTi | View protein in SMART SM00502, BBC, 1 hit SM00336, BBOX, 2 hits SM00184, RING, 1 hit SM00178, SAR, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATLVVNKLG AGVDSGRQGS RGTAVVKVLE CGVCEDVFSL QGDKVPRLLL
60 70 80 90 100
CGHTVCHDCL TRLPLHGRAI RCPFDRQVTD LGDSGVWGLK KNFALLELLE
110 120 130 140 150
RLQNGPIGQY GAAEESIGIS GESIIRCDED EAHLASVYCT VCATHLCSEC
160 170 180 190 200
SQVTHSTKTL AKHRRVPLAD KPHEKTMCSQ HQVHAIEFVC LEEGCQTSPL
210 220 230 240 250
MCCVCKEYGK HQGHKHSVLE PEANQIRASI LDMAHCIRTF TEEISDYSRK
260 270 280 290 300
LVGIVQHIEG GEQIVEDGIG MAHTEHVPGT AENARSCIRA YFYDLHETLC
310 320 330 340 350
RQEEMALSVV DAHVREKLIW LRQQQEDMTI LLSEVSAACL HCEKTLQQDD
360 370 380 390 400
CRVVLAKQEI TRLLETLQKQ QQQFTEVADH IQLDASIPVT FTKDNRVHIG
410 420 430 440 450
PKMEIRVVTL GLDGAGKTTI LFKLKQDEFM QPIPTIGFNV ETVEYKNLKF
460 470 480 490 500
TIWDVGGKHK LRPLWKHYYL NTQAVVFVVD SSHRDRISEA HSELAKLLTE
510 520 530 540 550
KELRDALLLI FANKQDVAGA LSVEEITELL SLHKLCCGRS WYIQGCDARS
560 570
GMGLYEGLDW LSRQLVAAGV LDVA
The sequence of this isoform differs from the canonical sequence as follows:
551-574: GMGLYEGLDWLSRQLVAAGVLDVA → VFQIICDQYTGKEVVTEKG
The sequence of this isoform differs from the canonical sequence as follows:
541-574: WYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA → CFSDNM
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RBN4 | D6RBN4_HUMAN | E3 ubiquitin-protein ligase TRIM23 | TRIM23 | 126 | Annotation score: | ||
D6R9E9 | D6R9E9_HUMAN | E3 ubiquitin-protein ligase TRIM23 | TRIM23 | 35 | Annotation score: | ||
D6RD22 | D6RD22_HUMAN | E3 ubiquitin-protein ligase TRIM23 | TRIM23 | 34 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048320 | 480 | D → N. Corresponds to variant dbSNP:rs34046496Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000297 | 541 – 574 | WYIQG…VLDVA → CFSDNM in isoform Gamma. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_000296 | 551 – 574 | GMGLY…VLDVA → VFQIICDQYTGKEVVTEKG in isoform Beta. 1 PublicationAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04510 mRNA Translation: AAA35940.1 AF230397 mRNA Translation: AAG50176.1 AF230398 mRNA Translation: AAG50177.1 AF230399 mRNA Translation: AAG50178.1 BC022510 mRNA Translation: AAH22510.1 |
CCDSi | CCDS3986.1 [P36406-3] CCDS3987.1 [P36406-1] CCDS43322.1 [P36406-2] |
PIRi | A46054 |
RefSeqi | NP_001647.1, NM_001656.3 [P36406-1] NP_150230.1, NM_033227.2 [P36406-2] NP_150231.1, NM_033228.2 [P36406-3] XP_016864933.1, XM_017009444.1 [P36406-2] |
Genome annotation databases
Ensembli | ENST00000231524.14; ENSP00000231524.9; ENSG00000113595.15 ENST00000274327.11; ENSP00000274327.7; ENSG00000113595.15 [P36406-3] ENST00000381018.7; ENSP00000370406.3; ENSG00000113595.15 [P36406-2] |
GeneIDi | 373 |
KEGGi | hsa:373 |
MANE-Selecti | ENST00000231524.14; ENSP00000231524.9; NM_001656.4; NP_001647.1 |
UCSCi | uc003jtw.4, human [P36406-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04510 mRNA Translation: AAA35940.1 AF230397 mRNA Translation: AAG50176.1 AF230398 mRNA Translation: AAG50177.1 AF230399 mRNA Translation: AAG50178.1 BC022510 mRNA Translation: AAH22510.1 |
CCDSi | CCDS3986.1 [P36406-3] CCDS3987.1 [P36406-1] CCDS43322.1 [P36406-2] |
PIRi | A46054 |
RefSeqi | NP_001647.1, NM_001656.3 [P36406-1] NP_150230.1, NM_033227.2 [P36406-2] NP_150231.1, NM_033228.2 [P36406-3] XP_016864933.1, XM_017009444.1 [P36406-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5VZV | X-ray | 1.81 | A/B/C | 1-123 | [»] | |
5VZW | X-ray | 2.28 | F/G | 1-123 | [»] | |
AlphaFoldDBi | P36406 | |||||
SMRi | P36406 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106868, 243 interactors |
CORUMi | P36406 |
IntActi | P36406, 232 interactors |
MINTi | P36406 |
STRINGi | 9606.ENSP00000231524 |
PTM databases
iPTMneti | P36406 |
PhosphoSitePlusi | P36406 |
Genetic variation databases
BioMutai | TRIM23 |
DMDMi | 543839 |
Proteomic databases
EPDi | P36406 |
jPOSTi | P36406 |
MassIVEi | P36406 |
MaxQBi | P36406 |
PaxDbi | P36406 |
PeptideAtlasi | P36406 |
PRIDEi | P36406 |
ProteomicsDBi | 55200 [P36406-1] 55201 [P36406-2] 55202 [P36406-3] |
Protocols and materials databases
Antibodypediai | 11584, 261 antibodies from 32 providers |
DNASUi | 373 |
Genome annotation databases
Ensembli | ENST00000231524.14; ENSP00000231524.9; ENSG00000113595.15 ENST00000274327.11; ENSP00000274327.7; ENSG00000113595.15 [P36406-3] ENST00000381018.7; ENSP00000370406.3; ENSG00000113595.15 [P36406-2] |
GeneIDi | 373 |
KEGGi | hsa:373 |
MANE-Selecti | ENST00000231524.14; ENSP00000231524.9; NM_001656.4; NP_001647.1 |
UCSCi | uc003jtw.4, human [P36406-1] |
Organism-specific databases
CTDi | 373 |
DisGeNETi | 373 |
GeneCardsi | TRIM23 |
HGNCi | HGNC:660, TRIM23 |
HPAi | ENSG00000113595, Low tissue specificity |
MIMi | 601747, gene |
neXtProti | NX_P36406 |
OpenTargetsi | ENSG00000113595 |
PharmGKBi | PA24943 |
VEuPathDBi | HostDB:ENSG00000113595 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0070, Eukaryota KOG4185, Eukaryota |
GeneTreei | ENSGT00940000158562 |
HOGENOMi | CLU_033905_0_0_1 |
InParanoidi | P36406 |
OMAi | WHIQGSS |
OrthoDBi | 1362554at2759 |
PhylomeDBi | P36406 |
TreeFami | TF320703 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.27, 2681 |
PathwayCommonsi | P36406 |
SignaLinki | P36406 |
SIGNORi | P36406 |
Miscellaneous databases
BioGRID-ORCSi | 373, 14 hits in 1121 CRISPR screens |
GeneWikii | TRIM23 |
GenomeRNAii | 373 |
Pharosi | P36406, Tbio |
PROi | PR:P36406 |
RNActi | P36406, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000113595, Expressed in corpus callosum and 226 other tissues |
ExpressionAtlasi | P36406, baseline and differential |
Genevisiblei | P36406, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003649, Bbox_C IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR024156, Small_GTPase_ARF IPR006689, Small_GTPase_ARF/SAR IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR013087, Znf_C2H2_type IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR11711, PTHR11711, 1 hit |
Pfami | View protein in Pfam PF00025, Arf, 1 hit PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SMARTi | View protein in SMART SM00502, BBC, 1 hit SM00336, BBOX, 2 hits SM00184, RING, 1 hit SM00178, SAR, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI23_HUMAN | |
Accessioni | P36406Primary (citable) accession number: P36406 Secondary accession number(s): Q9BZY4, Q9BZY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 206 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families