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Protein

ADP-ribosylation factor-like protein 3

Gene

ARL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31MagnesiumBy similarity1
Metal bindingi48MagnesiumBy similarity1
Binding sitei48GTPBy similarity1
Binding sitei70GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4
Nucleotide bindingi159 – 161GTPBy similarity3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: Ensembl
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Protein transport, Transport
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5624138 Trafficking of myristoylated proteins to the cilium

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 3
Gene namesi
Name:ARL3
Synonyms:ARFL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000138175.8
HGNCiHGNC:694 ARL3
MIMi604695 gene
neXtProtiNX_P36405

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31T → N: Enhances the interaction with RP2. 2 Publications1
Mutagenesisi71Q → L: Enhances the interaction with RP2. Does not induce a mitotic arrest resulting from the loss of the microtubule-based mitotic spindle. Induces release of myristoylated proteins from UNC119. Interacts with ARL2BP, GOLGA4, PDE6D and UNC119. 4 Publications1

Organism-specific databases

DisGeNETi403
OpenTargetsiENSG00000138175
PharmGKBiPA24987

Chemistry databases

DrugBankiDB03814 2-(N-Morpholino)-Ethanesulfonic Acid
DB04315 Guanosine-5'-Diphosphate

Polymorphism and mutation databases

BioMutaiARL3
DMDMi543851

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074562 – 182ADP-ribosylation factor-like protein 3Add BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei5PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP36405
MaxQBiP36405
PaxDbiP36405
PeptideAtlasiP36405
PRIDEiP36405
ProteomicsDBi55199

2D gel databases

REPRODUCTION-2DPAGEiIPI00003327
UCD-2DPAGEiP36405

PTM databases

iPTMnetiP36405
PhosphoSitePlusiP36405

Expressioni

Tissue specificityi

Expressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000138175 Expressed in 240 organ(s), highest expression level in pituitary gland
CleanExiHS_ARL3
GenevisibleiP36405 HS

Organism-specific databases

HPAiHPA036292
HPA063596

Interactioni

Subunit structurei

Interacts with RP2. The GTP-bound form interacts with PDE6D. Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with SYS1. The GTP-bound form interacts with ARL2BP and PDE6D. Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3. Microtubule-associated protein. Does not interact with TBCC. May interact with GOLGA4.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106896, 17 interactors
DIPiDIP-47537N
IntActiP36405, 28 interactors
MINTiP36405
STRINGi9606.ENSP00000260746

Structurei

3D structure databases

ProteinModelPortaliP36405
SMRiP36405
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0074 Eukaryota
ENOG410Y825 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP36405
KOiK07944
OMAiASEDITQ
OrthoDBiEOG091G0O6V
PhylomeDBiP36405
TreeFamiTF105463

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36405-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG
60 70 80 90 100
FNIKSVQSQG FKLNVWDIGG QRKIRPYWKN YFENTDILIY VIDSADRKRF
110 120 130 140 150
EETGQELAEL LEEEKLSCVP VLIFANKQDL LTAAPASEIA EGLNLHTIRD
160 170 180
RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK KK
Length:182
Mass (Da):20,456
Last modified:February 1, 1995 - v2
Checksum:iE5C1ACAD0BD55537
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01486934L → M1 PublicationCorresponds to variant dbSNP:rs1141895Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07151 mRNA Translation: AAA21654.1
AF493889 mRNA Translation: AAM12603.1
AK312525 mRNA Translation: BAG35424.1
CR407637 mRNA Translation: CAG28565.1
AL391121 Genomic DNA No translation available.
BC009841 mRNA Translation: AAH09841.1
CCDSiCCDS7538.1
PIRiA54869
RefSeqiNP_004302.1, NM_004311.3
XP_016871749.1, XM_017016260.1
UniGeneiHs.182215

Genome annotation databases

EnsembliENST00000260746; ENSP00000260746; ENSG00000138175
GeneIDi403
KEGGihsa:403
UCSCiuc001kwa.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07151 mRNA Translation: AAA21654.1
AF493889 mRNA Translation: AAM12603.1
AK312525 mRNA Translation: BAG35424.1
CR407637 mRNA Translation: CAG28565.1
AL391121 Genomic DNA No translation available.
BC009841 mRNA Translation: AAH09841.1
CCDSiCCDS7538.1
PIRiA54869
RefSeqiNP_004302.1, NM_004311.3
XP_016871749.1, XM_017016260.1
UniGeneiHs.182215

3D structure databases

ProteinModelPortaliP36405
SMRiP36405
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106896, 17 interactors
DIPiDIP-47537N
IntActiP36405, 28 interactors
MINTiP36405
STRINGi9606.ENSP00000260746

Chemistry databases

DrugBankiDB03814 2-(N-Morpholino)-Ethanesulfonic Acid
DB04315 Guanosine-5'-Diphosphate

PTM databases

iPTMnetiP36405
PhosphoSitePlusiP36405

Polymorphism and mutation databases

BioMutaiARL3
DMDMi543851

2D gel databases

REPRODUCTION-2DPAGEiIPI00003327
UCD-2DPAGEiP36405

Proteomic databases

EPDiP36405
MaxQBiP36405
PaxDbiP36405
PeptideAtlasiP36405
PRIDEiP36405
ProteomicsDBi55199

Protocols and materials databases

DNASUi403
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260746; ENSP00000260746; ENSG00000138175
GeneIDi403
KEGGihsa:403
UCSCiuc001kwa.4 human

Organism-specific databases

CTDi403
DisGeNETi403
EuPathDBiHostDB:ENSG00000138175.8
GeneCardsiARL3
HGNCiHGNC:694 ARL3
HPAiHPA036292
HPA063596
MIMi604695 gene
neXtProtiNX_P36405
OpenTargetsiENSG00000138175
PharmGKBiPA24987
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0074 Eukaryota
ENOG410Y825 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP36405
KOiK07944
OMAiASEDITQ
OrthoDBiEOG091G0O6V
PhylomeDBiP36405
TreeFamiTF105463

Enzyme and pathway databases

ReactomeiR-HSA-5624138 Trafficking of myristoylated proteins to the cilium

Miscellaneous databases

ChiTaRSiARL3 human
GeneWikiiARL3
GenomeRNAii403
PROiPR:P36405
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138175 Expressed in 240 organ(s), highest expression level in pituitary gland
CleanExiHS_ARL3
GenevisibleiP36405 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARL3_HUMAN
AccessioniPrimary (citable) accession number: P36405
Secondary accession number(s): B2R6C7, Q53X83, Q5JSM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: September 12, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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