UniProtKB - P36380 (CXB4_RAT)
Protein
Gap junction beta-4 protein
Gene
Gjb4
Organism
Rattus norvegicus (Rat)
Status
Functioni
Structural component of gap junctions (By similarity). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (By similarity).By similarity
GO - Molecular functioni
- gap junction channel activity Source: UniProtKB
GO - Biological processi
- cell-cell signaling Source: UniProtKB
- gap junction-mediated intercellular transport Source: UniProtKB
- olfactory behavior Source: RGD
- sensory perception of smell Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-190861, Gap junction assembly |
Names & Taxonomyi
Protein namesi | Recommended name: Gap junction beta-4 proteinAlternative name(s): Connexin-30.32 Publications Short name: Cx30.3 |
Gene namesi | Name:Gjb4 Synonyms:Cxn-30.3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621829, Gjb4 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- gap junction By similarity
Note: Colocalizes with GJB2 at gap junction plaques in the cochlea.By similarity
Plasma Membrane
- connexin complex Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Intramembranei | 2 – 13 | By similarityAdd BLAST | 12 | |
Topological domaini | 14 – 20 | CytoplasmicCurated | 7 | |
Transmembranei | 21 – 40 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 41 – 73 | ExtracellularCuratedAdd BLAST | 33 | |
Transmembranei | 74 – 94 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 95 – 130 | CytoplasmicCuratedAdd BLAST | 36 | |
Transmembranei | 131 – 151 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 152 – 184 | ExtracellularCuratedAdd BLAST | 33 | |
Transmembranei | 185 – 205 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 206 – 265 | CytoplasmicCuratedAdd BLAST | 60 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Gap junction, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057867 | 1 – 265 | Gap junction beta-4 proteinAdd BLAST | 265 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 53 ↔ 175 | By similarity | ||
Disulfide bondi | 60 ↔ 169 | By similarity | ||
Disulfide bondi | 64 ↔ 164 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P36380 |
PRIDEi | P36380 |
Expressioni
Tissue specificityi
Detected in adult heart, kidney, skin and cochlea, where it is detected in spiral ganglion, stria vascularis, spiral limbus and spiral ligament (at protein level).1 Publication
Interactioni
Subunit structurei
A hemichannel or connexon is composed of a hexamer of connexins. A functional gap junction is formed by the apposition of two hemichannels (By similarity). Forms heteromeric channels with GJB2 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000033893 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502R3YE, Eukaryota |
InParanoidi | P36380 |
OrthoDBi | 955506at2759 |
PhylomeDBi | P36380 |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002270, Connexin311 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit PTHR11984:SF30, PTHR11984:SF30, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01142, CONNEXINB5 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P36380-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNWGFLQGIL SGVNKYSTAL GRIWLSVVFI FRVLVYVVAA EEVWDDEQKD
60 70 80 90 100
FICNTKQPGC PNVCYDEFFP VSHVRLWALQ LILVTCPSLL VVMHVAYREE
110 120 130 140 150
RERKHRLKHG PDAPALYSNL SKKRGGLWWT YLLSLIFKAA VDSGFLYIFH
160 170 180 190 200
CIYKDYDMPR VVACSVQPCP HTVDCYISRP TEKKVFTYFM VVTAAICILL
210 220 230 240 250
NLSEVAYLVG KRCMEVFRPR RQKTSRRHQL PDTCPPYVIS KGHPQDESTV
260
LTKAGMATVD AGVYP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1M8I1 | F1M8I1_RAT | Gap junction protein | Gjb4 Cxnb, rCG_31398 | 265 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76168 mRNA Translation: CAA53762.1 |
PIRi | I60240, S38891 |
RefSeqi | NP_446436.1, NM_053984.2 |
Genome annotation databases
GeneIDi | 117055 |
KEGGi | rno:117055 |
UCSCi | RGD:621829, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76168 mRNA Translation: CAA53762.1 |
PIRi | I60240, S38891 |
RefSeqi | NP_446436.1, NM_053984.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000033893 |
Proteomic databases
PaxDbi | P36380 |
PRIDEi | P36380 |
Genome annotation databases
GeneIDi | 117055 |
KEGGi | rno:117055 |
UCSCi | RGD:621829, rat |
Organism-specific databases
CTDi | 127534 |
RGDi | 621829, Gjb4 |
Phylogenomic databases
eggNOGi | ENOG502R3YE, Eukaryota |
InParanoidi | P36380 |
OrthoDBi | 955506at2759 |
PhylomeDBi | P36380 |
Enzyme and pathway databases
Reactomei | R-RNO-190861, Gap junction assembly |
Miscellaneous databases
PROi | PR:P36380 |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002270, Connexin311 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit PTHR11984:SF30, PTHR11984:SF30, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01142, CONNEXINB5 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CXB4_RAT | |
Accessioni | P36380Primary (citable) accession number: P36380 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families