UniProtKB - P36371 (TAP2_MOUSE)
Antigen peptide transporter 2
Tap2
Functioni
ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation.
By similarityCatalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 16 | Inter-subunit salt bridge with TAPBPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 502 – 509 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type peptide antigen transporter activity Source: MGI
- ABC-type peptide transporter activity Source: GO_Central
- ADP binding Source: MGI
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- MHC class Ib protein binding Source: MGI
- MHC class I protein binding Source: MGI
- nucleotide binding Source: MGI
- peptide antigen binding Source: MGI
- protein-containing complex binding Source: MGI
- protein homodimerization activity Source: MGI
- TAP1 binding Source: UniProtKB
- TAP2 binding Source: MGI
- tapasin binding Source: UniProtKB
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- antigen processing and presentation of endogenous peptide antigen via MHC class I Source: MGI
- antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent Source: MGI
- antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent Source: MGI
- antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent Source: MGI
- cytosol to endoplasmic reticulum transport Source: MGI
- peptide antigen transport Source: MGI
- peptide transport Source: MGI
- positive regulation of antigen processing and presentation of peptide antigen via MHC class I Source: MGI
- positive regulation of T cell mediated cytotoxicity Source: MGI
- protection from natural killer cell mediated cytotoxicity Source: MGI
- protein-containing complex assembly Source: MGI
- protein transport Source: UniProtKB-KW
- response to molecule of bacterial origin Source: MGI
- transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1236974, ER-Phagosome pathway R-MMU-983170, Antigen Presentation: Folding, assembly and peptide loading of class I MHC |
Names & Taxonomyi
Protein namesi | Recommended name: Antigen peptide transporter 2 (EC:7.4.2.-By similarity)Short name: APT2 Alternative name(s): ATP-binding cassette sub-family B member 3 Histocompatibility antigen modifier 2 |
Gene namesi | Name:Tap2 Synonyms:Abcb3, Ham-2, Ham2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98484, Tap2 |
VEuPathDBi | HostDB:ENSMUSG00000024339 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: The transmembrane segments seem to form a pore in the membrane.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- integral component of endoplasmic reticulum membrane Source: MGI
- MHC class I peptide loading complex Source: MGI
- TAP complex Source: MGI
Nucleus
- nuclear speck Source: MGI
Other locations
- integral component of membrane Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | LumenalSequence analysis | 6 | |
Transmembranei | 7 – 27 | Helical; Name=1PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 28 – 56 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 57 – 77 | Helical; Name=2PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 78 – 98 | LumenalSequence analysisAdd BLAST | 21 | |
Transmembranei | 99 – 119 | Helical; Name=3PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 120 – 147 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 148 – 168 | Helical; Name=4PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 169 – 186 | LumenalSequence analysisAdd BLAST | 18 | |
Transmembranei | 187 – 207 | Helical; Name=5PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 208 – 265 | CytoplasmicSequence analysisAdd BLAST | 58 | |
Transmembranei | 266 – 286 | Helical; Name=6PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 287 – 292 | LumenalSequence analysis | 6 | |
Transmembranei | 293 – 313 | Helical; Name=7PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 314 – 373 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 374 – 394 | Helical; Name=8PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 395 – 407 | LumenalSequence analysisAdd BLAST | 13 | |
Transmembranei | 408 – 428 | Helical; Name=9PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 429 – 702 | CytoplasmicSequence analysisAdd BLAST | 274 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093330 | 1 – 702 | Antigen peptide transporter 2Add BLAST | 702 |
Proteomic databases
EPDi | P36371 |
PaxDbi | P36371 |
PeptideAtlasi | P36371 |
PRIDEi | P36371 |
ProteomicsDBi | 263068 |
PTM databases
iPTMneti | P36371 |
PhosphoSitePlusi | P36371 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024339, Expressed in thymus and 171 other tissues |
ExpressionAtlasi | P36371, baseline and differential |
Genevisiblei | P36371, MM |
Interactioni
Subunit structurei
Heterodimer of TAP1 and TAP2 (TAP1-TAP2). A component of the peptide loading complex (PLC), interacts via TAPBP with MHCI heterodimer; this interaction mediates peptide-MHCI assembly.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- MHC class Ib protein binding Source: MGI
- MHC class I protein binding Source: MGI
- protein homodimerization activity Source: MGI
- TAP1 binding Source: UniProtKB
- TAP2 binding Source: MGI
- tapasin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 203967, 6 interactors |
IntActi | P36371, 2 interactors |
STRINGi | 10090.ENSMUSP00000025197 |
Miscellaneous databases
RNActi | P36371, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 151 – 434 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 284 | |
Domaini | 467 – 701 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 235 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 300 – 388 | Part of the peptide-binding siteBy similarityAdd BLAST | 89 | |
Regioni | 413 – 432 | Part of the peptide-binding siteBy similarityAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0058, Eukaryota |
GeneTreei | ENSGT00940000160499 |
HOGENOMi | CLU_000604_84_3_1 |
InParanoidi | P36371 |
OMAi | ERQRMTI |
OrthoDBi | 684058at2759 |
PhylomeDBi | P36371 |
TreeFami | TF105197 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR013305, ABC_Tap-like IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR005293, Tap2/ABCB3 IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
PRINTSi | PR01897, TAP2PROTEIN |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR00958, 3a01208, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW
60 70 80 90 100
GLLKVGELLG LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG
110 120 130 140 150
WLLAGYGAVA LSWAVWAVLS PAGVQEKEPG QENRTLMKRL LKLSRPDLPF
160 170 180 190 200
LIAAFFFLVV AVWGETLIPR YSGRVIDILG GDFDPDAFAS AIFFMCLFSV
210 220 230 240 250
GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF QETKTGELNS
260 270 280 290 300
RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP
310 320 330 340 350
LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE
360 370 380 390 400
VSHYKEALER CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA
410 420 430 440 450
GEVTRGGLLS FLLYQEEVGQ YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP
460 470 480 490 500
NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP EKPVLQGLTF TLHPGTVTAL
510 520 530 540 550
VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY LHRQVVLVGQ
560 570 580 590 600
EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG
610 620 630 640 650
EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ
660 670 680 690 700
GDRTMLVIAH RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL
EA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6X8J6 | A6X8J6_MOUSE | Antigen peptide transporter 2 | Tap2 RP23-123G8.1-004 | 91 | Annotation score: | ||
A0A1B0GXB3 | A0A1B0GXB3_MOUSE | Antigen peptide transporter 2 | Tap2 | 24 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90459 mRNA Translation: AAA39609.1 |
CCDSi | CCDS28644.1 |
PIRi | A44135 |
RefSeqi | NP_035660.3, NM_011530.3 |
Genome annotation databases
Ensembli | ENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339 |
GeneIDi | 21355 |
KEGGi | mmu:21355 |
UCSCi | uc008cbx.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90459 mRNA Translation: AAA39609.1 |
CCDSi | CCDS28644.1 |
PIRi | A44135 |
RefSeqi | NP_035660.3, NM_011530.3 |
3D structure databases
AlphaFoldDBi | P36371 |
SMRi | P36371 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203967, 6 interactors |
IntActi | P36371, 2 interactors |
STRINGi | 10090.ENSMUSP00000025197 |
PTM databases
iPTMneti | P36371 |
PhosphoSitePlusi | P36371 |
Proteomic databases
EPDi | P36371 |
PaxDbi | P36371 |
PeptideAtlasi | P36371 |
PRIDEi | P36371 |
ProteomicsDBi | 263068 |
Protocols and materials databases
DNASUi | 21355 |
Genome annotation databases
Ensembli | ENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339 |
GeneIDi | 21355 |
KEGGi | mmu:21355 |
UCSCi | uc008cbx.2, mouse |
Organism-specific databases
CTDi | 6891 |
MGIi | MGI:98484, Tap2 |
VEuPathDBi | HostDB:ENSMUSG00000024339 |
Phylogenomic databases
eggNOGi | KOG0058, Eukaryota |
GeneTreei | ENSGT00940000160499 |
HOGENOMi | CLU_000604_84_3_1 |
InParanoidi | P36371 |
OMAi | ERQRMTI |
OrthoDBi | 684058at2759 |
PhylomeDBi | P36371 |
TreeFami | TF105197 |
Enzyme and pathway databases
Reactomei | R-MMU-1236974, ER-Phagosome pathway R-MMU-983170, Antigen Presentation: Folding, assembly and peptide loading of class I MHC |
Miscellaneous databases
BioGRID-ORCSi | 21355, 20 hits in 77 CRISPR screens |
ChiTaRSi | Tap2, mouse |
PROi | PR:P36371 |
RNActi | P36371, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024339, Expressed in thymus and 171 other tissues |
ExpressionAtlasi | P36371, baseline and differential |
Genevisiblei | P36371, MM |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR013305, ABC_Tap-like IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR005293, Tap2/ABCB3 IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
PRINTSi | PR01897, TAP2PROTEIN |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR00958, 3a01208, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAP2_MOUSE | |
Accessioni | P36371Primary (citable) accession number: P36371 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families