UniProtKB - P36370 (TAP1_RAT)
Antigen peptide transporter 1
Tap1
Functioni
ABC transporter associated with antigen processing (PubMed:17018292).
In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (By similarity).
Uses the chemical energy of ATP to export peptides against the concentration gradient (By similarity).
During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle (By similarity).
As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation (By similarity).
By similarity1 PublicationCatalytic activityi
- This reaction proceeds in the forward1 Publication direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 17 | Inter-subunit salt bridge with TAPBPBy similarity | 1 | |
Metal bindingi | 522 | MagnesiumCombined sources2 Publications | 1 | |
Binding sitei | 678 | ATPCombined sources2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 515 – 523 | ATPPROSITE-ProRule annotationCombined sources1 Publication | 9 | |
Nucleotide bindingi | 618 – 624 | ATPCombined sources2 Publications | 7 |
GO - Molecular functioni
- ABC-type peptide antigen transporter activity Source: RGD
- ABC-type peptide transporter activity Source: GO_Central
- ADP binding Source: RGD
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB
- ATP hydrolysis activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- MHC class Ib protein binding Source: RGD
- MHC class I protein binding Source: UniProtKB
- nucleotide binding Source: RGD
- peptide antigen binding Source: RGD
- peptide transmembrane transporter activity Source: RGD
- protein-containing complex binding Source: RGD
- protein homodimerization activity Source: UniProtKB
- TAP1 binding Source: UniProtKB
- TAP2 binding Source: UniProtKB
- tapasin binding Source: RGD
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- antigen processing and presentation of endogenous peptide antigen via MHC class I Source: RGD
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: InterPro
- cytosol to endoplasmic reticulum transport Source: RGD
- defense response Source: RGD
- peptide antigen transport Source: RGD
- peptide transport Source: RGD
- protection from natural killer cell mediated cytotoxicity Source: RGD
- protein transport Source: UniProtKB-KW
- transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-1236974, ER-Phagosome pathway R-RNO-983170, Antigen Presentation: Folding, assembly and peptide loading of class I MHC |
Names & Taxonomyi
Protein namesi | Recommended name: Antigen peptide transporter 1 (EC:7.4.2.-1 Publication)Short name: APT1 Alternative name(s): ATP-binding cassette sub-family B member 2 Peptide transporter TAP1 |
Gene namesi | Name:Tap1 Synonyms:Abcb2, Mtp1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3817, Tap1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: The transmembrane segments seem to form a pore in the membrane.
Endoplasmic reticulum
- integral component of endoplasmic reticulum membrane Source: RGD
- MHC class I peptide loading complex Source: UniProtKB
- TAP complex Source: UniProtKB
Other locations
- integral component of membrane Source: RGD
- intracellular membrane-bounded organelle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 29 | Helical; Name=1PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 30 – 38 | LumenalSequence analysis | 9 | |
Transmembranei | 39 – 60 | Helical; Name=2PROSITE-ProRule annotationAdd BLAST | 22 | |
Topological domaini | 61 – 67 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 68 – 88 | Helical; Name=3PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 89 – 110 | LumenalSequence analysisAdd BLAST | 22 | |
Transmembranei | 111 – 131 | Helical; Name=4PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 132 – 163 | CytoplasmicSequence analysisAdd BLAST | 32 | |
Transmembranei | 164 – 184 | Helical; Name=5PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 185 – 204 | LumenalSequence analysisAdd BLAST | 20 | |
Transmembranei | 205 – 225 | Helical; Name=6PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 226 – 275 | CytoplasmicSequence analysisAdd BLAST | 50 | |
Transmembranei | 276 – 296 | Helical; Name=7PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 297 – 305 | LumenalSequence analysis | 9 | |
Transmembranei | 306 – 326 | Helical; Name=8PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 327 – 395 | CytoplasmicSequence analysisAdd BLAST | 69 | |
Transmembranei | 396 – 416 | Helical; Name=9PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 417 – 420 | LumenalSequence analysis | 4 | |
Transmembranei | 421 – 441 | Helical; Name=10PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 442 – 725 | CytoplasmicSequence analysisAdd BLAST | 284 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 621 – 622 | SG → AV: Complete loss of ATPase activity; when associated with N-645. Impairs peptide loading onto MHCI. 1 Publication | 2 | |
Mutagenesisi | 645 | D → E: Increases ATPase activity by 8-fold; when associated with H-678. 1 Publication | 1 | |
Mutagenesisi | 645 | D → N: Complete loss of ATPase activity; when associated with A-621 and V-622. 1 Publication | 1 | |
Mutagenesisi | 645 | D → Q: Impairs ATPase activity; when associated with H-678. 1 Publication | 1 | |
Mutagenesisi | 651 | D → A or N: Decreases ATP-driven nucleotide binding domain (NBD) dimerization. 1 Publication | 1 | |
Mutagenesisi | 678 | Q → H: Increases ATPase activity by 8-fold; when associated with E-645. Impairs ATPase activity; when associated with Q-645. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093328 | 1 – 725 | Antigen peptide transporter 1Add BLAST | 725 |
Proteomic databases
jPOSTi | P36370 |
PaxDbi | P36370 |
PTM databases
iPTMneti | P36370 |
PhosphoSitePlusi | P36370 |
Interactioni
Subunit structurei
Heterodimer of TAP1 and TAP2 (TAP1-TAP2). A component of the peptide loading complex (PLC), interacts via TAPBP with MHCI heterodimer; this interaction mediates peptide-MHCI assembly.
Interacts with PSMB5 and PSMB8.
By similarityBinary interactionsi
P36370
With | #Exp. | IntAct |
---|---|---|
Tapbp [Q99JC6] | 2 | EBI-11303917,EBI-11304538 |
GO - Molecular functioni
- MHC class Ib protein binding Source: RGD
- MHC class I protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- TAP1 binding Source: UniProtKB
- TAP2 binding Source: UniProtKB
- tapasin binding Source: RGD
Protein-protein interaction databases
IntActi | P36370, 3 interactors |
STRINGi | 10116.ENSRNOP00000000529 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P36370 |
SMRi | P36370 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P36370 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 164 – 447 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 284 | |
Domaini | 480 – 719 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 240 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 352 – 397 | Part of the peptide-binding siteBy similarityAdd BLAST | 46 | |
Regioni | 430 – 464 | Part of the peptide-binding siteBy similarityAdd BLAST | 35 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0058, Eukaryota |
InParanoidi | P36370 |
PhylomeDBi | P36370 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR013305, ABC_Tap-like IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR013306, Tap1/ABCB2 IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit PTHR24221:SF249, PTHR24221:SF249, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR00958, 3a01208, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAAHAWPTAA LLLLLVDWLL LRPVLPGIFS LLVPEVPLLR VWAVGLSRWA
60 70 80 90 100
ILGLGVRGVL GVTAGARGWL AALQPLVAAL GLALPGLASF RKLSAWGALR
110 120 130 140 150
EGDNAGLLHW NSRLDAFVLS YVAALPAAAL WHKLGGFWAP SGHKGAGDML
160 170 180 190 200
CRMLGFLDSK KGRLHLVLVL LILSCLGEMA IPFFTGRITD WILQDKTAPS
210 220 230 240 250
FARNMWLMCI LTIASTVLEF AGDGIYNITM GHMHSRVHGE VFRAVLHQET
260 270 280 290 300
GFFLKNPTGS ITSRVTEDTS NVCESISDKL NLFLWYLGRG LCLLAFMIWG
310 320 330 340 350
SFYLTVVTLL SLPLLFLLPR RLGKVYQSLA VKVQESLAKS TQVALEALSA
360 370 380 390 400
MPTVRSFANE EGEAQKFRQK LEEMKPLNKK EALAYVTEVW TMSVSGMLLK
410 420 430 440 450
VGILYLGGQL VVRGAVSSGN LVSFVLYQLQ FTRAVEVLLS IYPSMQKSVG
460 470 480 490 500
ASEKIFEYLD RTPCSPLSGS LAPLNMKGLV KFQDVSFAYP NHPNVQVLQG
510 520 530 540 550
LTFTLYPGKV TALVGPNGSG KSTVAALLQN LYQPTGGKVL LDGEPLVQYD
560 570 580 590 600
HHYLHTQVAA VGQEPLLFGR SFRENIAYGL TRTPTMEEIT AVAMESGAHD
610 620 630 640 650
FISGFPQGYD TEVGETGNQL SGGQRQAVAL ARALIRKPRL LILDDATSAL
660 670 680 690 700
DAGNQLRVQR LLYESPEWAS RTVLLITQQL SLAERAHHIL FLKEGSVCEQ
710 720
GTHLQLMERG GCYRSMVEAL AAPSD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketP97949 | P97949_RAT | Antigen peptide transporter 1 | Tap1 Arrb2-ps, Hla-dma, Hla-dmb, Psmb9, RT1-Ba | 725 | Annotation score: | ||
A0A8I6GGZ1 | A0A8I6GGZ1_RAT | Antigen peptide transporter 1 | Tap1 | 697 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57523 mRNA Translation: CAA40742.1 Different initiation. Y10231 mRNA Translation: CAA71280.1 |
PIRi | S13426 |
Genome annotation databases
UCSCi | RGD:3817, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57523 mRNA Translation: CAA40742.1 Different initiation. Y10231 mRNA Translation: CAA71280.1 |
PIRi | S13426 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IXE | X-ray | 2.00 | A/D | 465-725 | [»] | |
2IXF | X-ray | 2.00 | A/B/C/D | 465-725 | [»] | |
2IXG | X-ray | 2.70 | A | 465-725 | [»] | |
4K8O | X-ray | 2.65 | A | 465-725 | [»] | |
AlphaFoldDBi | P36370 | |||||
SMRi | P36370 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P36370, 3 interactors |
STRINGi | 10116.ENSRNOP00000000529 |
PTM databases
iPTMneti | P36370 |
PhosphoSitePlusi | P36370 |
Proteomic databases
jPOSTi | P36370 |
PaxDbi | P36370 |
Genome annotation databases
UCSCi | RGD:3817, rat |
Organism-specific databases
RGDi | 3817, Tap1 |
Phylogenomic databases
eggNOGi | KOG0058, Eukaryota |
InParanoidi | P36370 |
PhylomeDBi | P36370 |
Enzyme and pathway databases
Reactomei | R-RNO-1236974, ER-Phagosome pathway R-RNO-983170, Antigen Presentation: Folding, assembly and peptide loading of class I MHC |
Miscellaneous databases
EvolutionaryTracei | P36370 |
PROi | PR:P36370 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR013305, ABC_Tap-like IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR013306, Tap1/ABCB2 IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit PTHR24221:SF249, PTHR24221:SF249, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR00958, 3a01208, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAP1_RAT | |
Accessioni | P36370Primary (citable) accession number: P36370 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families