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Protein

Antigen peptide transporter 1

Gene

Tap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi515 – 522ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ADP binding Source: RGD
  • ATPase activity Source: RGD
  • ATP binding Source: UniProtKB
  • MHC class I protein binding Source: UniProtKB
  • nucleotide binding Source: RGD
  • peptide antigen-transporting ATPase activity Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: RGD

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: InterPro
  • peptide antigen transport Source: RGD
  • peptide transport Source: RGD
  • protection from natural killer cell mediated cytotoxicity Source: RGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processAdaptive immunity, Immunity, Peptide transport, Protein transport, Transport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 1
Short name:
APT1
Alternative name(s):
ATP-binding cassette sub-family B member 2
Peptide transporter TAP1
Gene namesi
Name:Tap1
Synonyms:Abcb2, Mtp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3817 Tap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini30 – 38LumenalSequence analysis9
Transmembranei39 – 60Helical; Name=2PROSITE-ProRule annotationAdd BLAST22
Topological domaini61 – 67CytoplasmicSequence analysis7
Transmembranei68 – 88Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini89 – 110LumenalSequence analysisAdd BLAST22
Transmembranei111 – 131Helical; Name=4PROSITE-ProRule annotationAdd BLAST21
Topological domaini132 – 163CytoplasmicSequence analysisAdd BLAST32
Transmembranei164 – 184Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini185 – 204LumenalSequence analysisAdd BLAST20
Transmembranei205 – 225Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini226 – 275CytoplasmicSequence analysisAdd BLAST50
Transmembranei276 – 296Helical; Name=7PROSITE-ProRule annotationAdd BLAST21
Topological domaini297 – 305LumenalSequence analysis9
Transmembranei306 – 326Helical; Name=8PROSITE-ProRule annotationAdd BLAST21
Topological domaini327 – 395CytoplasmicSequence analysisAdd BLAST69
Transmembranei396 – 416Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini417 – 420LumenalSequence analysis4
Transmembranei421 – 441Helical; Name=10PROSITE-ProRule annotationAdd BLAST21
Topological domaini442 – 725CytoplasmicSequence analysisAdd BLAST284

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933281 – 725Antigen peptide transporter 1Add BLAST725

Proteomic databases

PaxDbiP36370
PRIDEiP36370

PTM databases

iPTMnetiP36370
PhosphoSitePlusiP36370

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2. Interacts with PSMB5 and PSMB8 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TapbpQ99JC62EBI-11303917,EBI-11304538

GO - Molecular functioni

  • MHC class I protein binding Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: RGD

Protein-protein interaction databases

IntActiP36370, 3 interactors
STRINGi10116.ENSRNOP00000000529

Structurei

Secondary structure

1725
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni466 – 469Combined sources4
Beta strandi480 – 487Combined sources8
Beta strandi498 – 505Combined sources8
Beta strandi510 – 514Combined sources5
Helixi521 – 528Combined sources8
Beta strandi535 – 541Combined sources7
Helixi546 – 548Combined sources3
Helixi551 – 557Combined sources7
Beta strandi558 – 561Combined sources4
Beta strandi569 – 571Combined sources3
Helixi572 – 577Combined sources6
Helixi586 – 595Combined sources10
Helixi599 – 604Combined sources6
Helixi608 – 610Combined sources3
Helixi615 – 617Combined sources3
Helixi622 – 634Combined sources13
Beta strandi639 – 645Combined sources7
Turni646 – 649Combined sources4
Helixi652 – 664Combined sources13
Turni666 – 670Combined sources5
Beta strandi671 – 676Combined sources6
Helixi680 – 683Combined sources4
Beta strandi687 – 693Combined sources7
Beta strandi696 – 701Combined sources6
Helixi703 – 709Combined sources7
Helixi712 – 718Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IXEX-ray2.00A/D465-725[»]
2IXFX-ray2.00A/B/C/D465-725[»]
2IXGX-ray2.70A465-725[»]
4K8OX-ray2.65A465-725[»]
ProteinModelPortaliP36370
SMRiP36370
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36370

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini164 – 447ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST284
Domaini480 – 719ABC transporterPROSITE-ProRule annotationAdd BLAST240

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni352 – 397Involved in peptide-binding siteBy similarityAdd BLAST46
Regioni430 – 464Involved in peptide-binding siteBy similarityAdd BLAST35

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058 Eukaryota
COG1132 LUCA
HOVERGENiHBG008358
InParanoidiP36370
PhylomeDBiP36370

Family and domain databases

Gene3Di1.20.1560.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR013305 ABC_Tap-like
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR013306 Tap1/ABCB2
PANTHERiPTHR24221:SF249 PTHR24221:SF249, 1 hit
PfamiView protein in Pfam
PF00664 ABC_membrane, 1 hit
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF90123 SSF90123, 1 hit
TIGRFAMsiTIGR00958 3a01208, 1 hit
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit

Sequencei

Sequence statusi: Complete.

P36370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHAWPTAA LLLLLVDWLL LRPVLPGIFS LLVPEVPLLR VWAVGLSRWA
60 70 80 90 100
ILGLGVRGVL GVTAGARGWL AALQPLVAAL GLALPGLASF RKLSAWGALR
110 120 130 140 150
EGDNAGLLHW NSRLDAFVLS YVAALPAAAL WHKLGGFWAP SGHKGAGDML
160 170 180 190 200
CRMLGFLDSK KGRLHLVLVL LILSCLGEMA IPFFTGRITD WILQDKTAPS
210 220 230 240 250
FARNMWLMCI LTIASTVLEF AGDGIYNITM GHMHSRVHGE VFRAVLHQET
260 270 280 290 300
GFFLKNPTGS ITSRVTEDTS NVCESISDKL NLFLWYLGRG LCLLAFMIWG
310 320 330 340 350
SFYLTVVTLL SLPLLFLLPR RLGKVYQSLA VKVQESLAKS TQVALEALSA
360 370 380 390 400
MPTVRSFANE EGEAQKFRQK LEEMKPLNKK EALAYVTEVW TMSVSGMLLK
410 420 430 440 450
VGILYLGGQL VVRGAVSSGN LVSFVLYQLQ FTRAVEVLLS IYPSMQKSVG
460 470 480 490 500
ASEKIFEYLD RTPCSPLSGS LAPLNMKGLV KFQDVSFAYP NHPNVQVLQG
510 520 530 540 550
LTFTLYPGKV TALVGPNGSG KSTVAALLQN LYQPTGGKVL LDGEPLVQYD
560 570 580 590 600
HHYLHTQVAA VGQEPLLFGR SFRENIAYGL TRTPTMEEIT AVAMESGAHD
610 620 630 640 650
FISGFPQGYD TEVGETGNQL SGGQRQAVAL ARALIRKPRL LILDDATSAL
660 670 680 690 700
DAGNQLRVQR LLYESPEWAS RTVLLITQQL SLAERAHHIL FLKEGSVCEQ
710 720
GTHLQLMERG GCYRSMVEAL AAPSD
Length:725
Mass (Da):79,150
Last modified:November 1, 1997 - v2
Checksum:i3FA7215D0AC22EE0
GO

Sequence cautioni

The sequence CAA40742 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57523 mRNA Translation: CAA40742.1 Different initiation.
Y10231 mRNA Translation: CAA71280.1
PIRiS13426
UniGeneiRn.10763

Genome annotation databases

UCSCiRGD:3817 rat

Similar proteinsi

Entry informationi

Entry nameiTAP1_RAT
AccessioniPrimary (citable) accession number: P36370
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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