UniProtKB - P36347 (M1_I02A0)
Protein
Matrix protein 1
Gene
M
Organism
Influenza A virus (strain A/Chicken/Brescia/1902 H7N7)
Status
Functioni
Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.UniRule annotation
Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.UniRule annotation
Miscellaneous
Most abundant protein in virion. When expressed alone can form virus-like particles in transfected cells.UniRule annotation
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
- structural constituent of virion Source: UniProtKB-UniRule
GO - Biological processi
- viral budding from plasma membrane Source: UniProtKB-UniRule
Keywordsi
Molecular function | RNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Matrix protein 1UniRule annotationShort name: M1UniRule annotation |
Gene namesi | Name:MUniRule annotation |
Organismi | Influenza A virus (strain A/Chicken/Brescia/1902 H7N7) |
Taxonomic identifieri | 36418 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Alphainfluenzavirus › |
Virus hosti | Aves [TaxID: 8782] Equus caballus (Horse) [TaxID: 9796] Homo sapiens (Human) [TaxID: 9606] Phocidae (true seals) [TaxID: 9709] |
Proteomesi |
|
Subcellular locationi
- Virion membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
- Host nucleus UniRule annotation
Keywords - Cellular componenti
Host nucleus, Membrane, Viral matrix protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000078853 | 1 – 252 | Matrix protein 1Add BLAST | 252 |
Interactioni
Subunit structurei
Homodimer and homomultimer.
Interacts with NEP. Binds ribonucleocapsid by both interacting with genomic RNA and NP protein. May interact with HA and NA. Cannot bind NP without genomic RNA.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 164 | Membrane-bindingUniRule annotationAdd BLAST | 164 | |
Regioni | 165 – 252 | RNP-bindingUniRule annotationAdd BLAST | 88 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 101 – 105 | Nuclear localization signalUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the influenza viruses Matrix protein M1 family.UniRule annotation
Family and domain databases
Gene3Di | 1.10.10.180, 1 hit 1.20.91.10, 1 hit |
HAMAPi | MF_04068, INFV_M1, 1 hit |
InterProi | View protein in InterPro IPR036039, Flu_matrix_M1 IPR013188, Flu_matrix_M1_C IPR001561, Flu_matrix_M1_N IPR015423, Flu_matrix_M1_N_sub1 IPR015799, Flu_matrix_M1_N_sub2 IPR037533, INFV_M1 |
Pfami | View protein in Pfam PF00598, Flu_M1, 1 hit PF08289, Flu_M1_C, 1 hit |
SMARTi | View protein in SMART SM00759, Flu_M1_C, 1 hit |
SUPFAMi | SSF48145, SSF48145, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Only the first 9 residues are shared by the 2 isoforms.
Isoform M1 (identifier: P36347-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLLTEVETY VLSIVPSGPL KAEIAQRLED VFAGKNTDLE ALMEWLKTRP
60 70 80 90 100
ILSPLTKGIL GFVFTLTVPS ERGLQRRRFV QNALNGNGDP NNMDRAVKLY
110 120 130 140 150
KKLKREITFH GAKEVALSYS TGALASCMGL IYNRMGTVTT EVALGLVCAT
160 170 180 190 200
CEQIADSQHR SHRQMVTTTN PLIRHENRMV LTSTTAKAME QMAGSSEQAA
210 220 230 240 250
EAMEVASQAR QMVQAMRTIG THPSSSAGLK DDLLENLQAY QKRMGVQMQR
FK
Isoform M2 (identifier: P36348-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P36348.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | V → L in AAA56804 (PubMed:1530908). | 1 | |
Sequence conflicti | 98 | K → M in AAA56804 (PubMed:1530908). | 1 | |
Sequence conflicti | 111 | G → R in AAA56804 (PubMed:1530908). | 1 | |
Sequence conflicti | 182 | T → A in AAA56804 (PubMed:1530908). | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37795 Genomic RNA Translation: AAA56804.1 CY015054 Genomic RNA Translation: ABI85068.1 |
PIRi | B45539 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37795 Genomic RNA Translation: AAA56804.1 CY015054 Genomic RNA Translation: ABI85068.1 |
PIRi | B45539 |
3D structure databases
SMRi | P36347 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 1.10.10.180, 1 hit 1.20.91.10, 1 hit |
HAMAPi | MF_04068, INFV_M1, 1 hit |
InterProi | View protein in InterPro IPR036039, Flu_matrix_M1 IPR013188, Flu_matrix_M1_C IPR001561, Flu_matrix_M1_N IPR015423, Flu_matrix_M1_N_sub1 IPR015799, Flu_matrix_M1_N_sub2 IPR037533, INFV_M1 |
Pfami | View protein in Pfam PF00598, Flu_M1, 1 hit PF08289, Flu_M1_C, 1 hit |
SMARTi | View protein in SMART SM00759, Flu_M1_C, 1 hit |
SUPFAMi | SSF48145, SSF48145, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | M1_I02A0 | |
Accessioni | P36347Primary (citable) accession number: P36347 Secondary accession number(s): Q0A2L3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | March 6, 2007 | |
Last modified: | October 7, 2020 | |
This is version 103 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |