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UniProtKB - P36300 (SPIKE_CVCAI)

Entry version 95 (02 Dec 2020)
Sequence version 1 (01 Jun 1994)
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Protein

Spike glycoprotein

Gene

S

Organism
Canine coronavirus (strain Insavc-1) (CCoV) (Canine enteric coronavirus)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.UniRule annotation

Caution

In contrast to beta- and gammacoronaviruses, S glycoprotein is not cleaved into S1 and S2.UniRule annotation

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processHost-virus interaction, Viral attachment to host cell, Virulence, Virus entry into host cell

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Spike glycoproteinUniRule annotation
Short name:
S glycoproteinUniRule annotation
Alternative name(s):
E2UniRule annotation
Peplomer proteinUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCanine coronavirus (strain Insavc-1) (CCoV) (Canine enteric coronavirus)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri36391 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiViruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Alphacoronavirus › Tegacovirus ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiCanis lupus familiaris (Dog) (Canis familiaris) [TaxID: 9615]

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini32 – 1392Virion surfaceUniRule annotationAdd BLAST1361
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei1393 – 1412HelicalUniRule annotationAdd BLAST20
Topological domaini1413 – 1451IntravirionUniRule annotationAdd BLAST39

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Host membrane, Membrane, Viral envelope protein, Virion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 31UniRule annotationAdd BLAST31
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000003720232 – 1451Spike glycoproteinUniRule annotationAdd BLAST1420

Keywords - PTMi

Glycoprotein

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins.

Interacts with host ANPEP.

UniRule annotation2 Publications

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P36300

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni32 – 779S1UniRule annotationAdd BLAST748
Regioni660 – 803Interaction with host ANPEPUniRule annotationAdd BLAST144
Regioni780 – 1451S2UniRule annotationAdd BLAST672
Regioni1024 – 1045Fusion peptideUniRule annotationAdd BLAST22
Regioni1039 – 1158Heptad repeat 1 (HR1)PROSITE-ProRule annotationAdd BLAST120
Regioni1307 – 1404Heptad repeat 2 (HR2)PROSITE-ProRule annotationAdd BLAST98

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili1106 – 1150UniRule annotationAdd BLAST45
Coiled coili1340 – 1382UniRule annotationAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi1447 – 1451KxHxxUniRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1413 – 1434Cys-richAdd BLAST22

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The KxHxx motif seems to function as an ER retrieval signal.UniRule annotation

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the alphacoronaviruses spike protein family.UniRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

HAMAP database of protein families

More...HAMAPi		MF_04200, ALPHA_CORONA_SPIKE, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR042552, ALPHA_CORONA_SPIKE
IPR043607, CoV_S1_C
IPR043473, S2_sf_CoV
IPR002551, Spike_S1_CoV
IPR002552, Spike_S2_CoV
IPR043614, Spike_S2_CoV_C

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01600, CoV_S1, 1 hit
PF19209, CoV_S1_C, 1 hit
PF01601, CoV_S2, 1 hit
PF19214, CoV_S2_C, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF111474, SSF111474, 2 hits

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51923, COV_S2_HR1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P36300-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIVLTLCLFL FLYSSVSCTS NNDCVQVNVT QLPGNENIIK DFLFQNFKEE 
        60         70         80         90        100
GSLVVGGYYP TEVWYNCSTT QQTTAYKYFS NIHAFYFDME AMENSTGNAR 
       110        120        130        140        150
GKPLLVHVHG NPVSIIVYIS AYRDDVQFRP LLKHGLLCIT KNDTVDYNSF 
       160        170        180        190        200
TINQWRDICL GDDRKIPFSV VPTDNGTKLF GLEWNDDYVT AYISDESHRL 
       210        220        230        240        250
NINNNWFNNV TLLYSRTSTA TWQHSAAYVY QGVSNFTYYK LNKTAGLKSY 
       260        270        280        290        300
ELCEDYEYCT GYATNVFAPT SGGYIPDGFS FNNWFMLTNS STFVSGRFVT 
       310        320        330        340        350
NQPLLVNCLW PVPSFGVAAQ EFCFEGAQFS QCNGVSLNNT VDVIRFNLNF 
       360        370        380        390        400
TTDVQSGMGA TVFSLNTTGG VILEISCYND TVSESSFYSY GEIPFGVTDG 
       410        420        430        440        450
PRYCYVLYNG TALKYLGTLP PSVKEIAISK WGHFYINGYN FFSTFPIDCI 
       460        470        480        490        500
AFNLTTGASG AFWTIAYTSY TEALVQVENT AIKKVTYCNS HINNIKCSQL 
       510        520        530        540        550
TANLQNGFYP VASSEVGLVN KSVVLLPSFY SHTSVNITID LGMKRSVTVT 
       560        570        580        590        600
IASPLSNITL PMQDNNIDVY CIRSNQFSVY VHSTCKSSLW DNNFNSACTD 
       610        620        630        640        650
VLDATAVIKT GTCPFSFDKL NNYLTFNKFC LSLNPVGANC KLDVAARTRT 
       660        670        680        690        700
NEQVFGSLYV IYEEGDNIVG VPSDNSGLHD LSVLHLDSCT DYNIYGRTGV 
       710        720        730        740        750
GIIRKTNSTL LSGLYYTSLS GDLLGFKNVS DGVVYSVTPC DVSAQAAVID 
       760        770        780        790        800
GAIVGAMTSI NSELLGLTHW TTTPNFYYYS IYNYTNVMNR GTAIDNDIDC 
       810        820        830        840        850
EPIITYSNIG VCKNGALVFI NVTHSDGDVQ PISTGNVTIP TNFTISVQVE 
       860        870        880        890        900
YIQVYTTPVS IDCARYVCNG NPRCNKLLTQ YVSACQTIEQ ALAMGARLEN 
       910        920        930        940        950
MEIDSMLFVS ENALKLASVE AFNSTENLDP IYKEWPNIGG SWLGGLKDIL 
       960        970        980        990       1000
PSHNSKRKYR SAIEDLLFDK VVTSGLGTVD EDYKRSAGGY DIADLVCARY 
      1010       1020       1030       1040       1050
YNGIMVLPGV ANDDKMTMYT ASLTGGITLG ALSGGAVAIP FAVAVQARLN 
      1060       1070       1080       1090       1100
YVALQTDVLN KNQQILANAF NQAIGNITQA FGKVNDAIHQ TSKGLATVAK 
      1110       1120       1130       1140       1150
ALAKVQDVVN TQGQALSHLT VQLQNNFQAI SSSISDIYNR LDELSADAQV 
      1160       1170       1180       1190       1200
DRLITGRLTA LNAFVSQTLT RQAEVRASRQ LAKDKVNECV RSQSQRFGFC 
      1210       1220       1230       1240       1250
GNGTHLFSLA NAAPNGMIFF HTVLLPTAYE TVTAWSGICA SDGSRTFGLV 
      1260       1270       1280       1290       1300
VEDVQLTLFR NLDEKFYLTP RTMYQPRVAT SSDFVQIEGC DVLFVNGTVI 
      1310       1320       1330       1340       1350
ELPSIIPDYI DINQTVQDIL ENFRPNWTVP ELPLDIFHAT YLNLTGEIND 
      1360       1370       1380       1390       1400
LEFRSEKLHN TTVELAILID NINNTLVNLE WLNRIETYVK WPWYVWLLIG 
      1410       1420       1430       1440       1450
LVVIFCIPIL LFCCCSTGCC GCIGCLGSCC HSICSRGQFE SYEPIEKVHV 

H
Length:1,451
Mass (Da):160,468
Last modified:June 1, 1994 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF3E6ECA8B914CE6C
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
D13096 Genomic RNA Translation: BAA02408.1

Protein sequence database of the Protein Information Resource

More...PIRi		JQ1719

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13096 Genomic RNA Translation: BAA02408.1
PIRiJQ1719

3D structure databases

SMRiP36300
ModBaseiSearch...

Family and domain databases

HAMAPiMF_04200, ALPHA_CORONA_SPIKE, 1 hit
InterProiView protein in InterPro
IPR042552, ALPHA_CORONA_SPIKE
IPR043607, CoV_S1_C
IPR043473, S2_sf_CoV
IPR002551, Spike_S1_CoV
IPR002552, Spike_S2_CoV
IPR043614, Spike_S2_CoV_C
PfamiView protein in Pfam
PF01600, CoV_S1, 1 hit
PF19209, CoV_S1_C, 1 hit
PF01601, CoV_S2, 1 hit
PF19214, CoV_S2_C, 1 hit
SUPFAMiSSF111474, SSF111474, 2 hits
PROSITEiView protein in PROSITE
PS51923, COV_S2_HR1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPIKE_CVCAI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P36300
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: December 2, 2020
This is version 95 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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