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Protein

Glutathione hydrolase 5 proenzyme

Gene

GGT5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4).

Catalytic activityi

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.
Glutathione + H2O = L-cysteinylglycine + L-glutamate.
Leukotriene C4 + H2O = leukotriene D4 + L-glutamate.

Pathwayi: glutathione metabolism

This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.

Pathwayi: leukotriene D4 biosynthesis

This protein is involved in the pathway leukotriene D4 biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway leukotriene D4 biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110GlutamateBy similarity1
Active sitei388NucleophileBy similarity1
Binding sitei406GlutamateBy similarity1
Binding sitei427GlutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Hydrolase, Protease, Transferase
Biological processGlutathione biosynthesis, Leukotriene biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:HS01949-MONOMER
BRENDAi2.3.2.2 2681
3.4.19.13 2681
ReactomeiR-HSA-174403 Glutathione synthesis and recycling
R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-HSA-5423646 Aflatoxin activation and detoxification
UniPathwayi
UPA00204

UPA00880

Protein family/group databases

MEROPSiT03.002

Chemistry databases

SwissLipidsiSLP:000001455

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione hydrolase 5 proenzyme (EC:3.4.19.13)
Alternative name(s):
Gamma-glutamyl transpeptidase-related enzyme
Short name:
GGT-rel
Gamma-glutamyltransferase 5 (EC:2.3.2.2)
Short name:
GGT 5
Gamma-glutamyltransferase-like activity 1
Gamma-glutamyltranspeptidase 5
Leukotriene-C4 hydrolase (EC:3.4.19.14)
Cleaved into the following 2 chains:
Gene namesi
Name:GGT5
Synonyms:GGTLA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000099998.17
HGNCiHGNC:4260 GGT5
MIMi137168 gene
neXtProtiNX_P36269

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini30 – 586ExtracellularSequence analysisAdd BLAST557

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi2687
OpenTargetsiENSG00000099998
PharmGKBiPA162389442

Polymorphism and mutation databases

BioMutaiGGT5
DMDMi116242493

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000110721 – 387Glutathione hydrolase 5 heavy chainBy similarityAdd BLAST387
ChainiPRO_0000011073388 – 586Glutathione hydrolase 5 light chainBy similarityAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi98N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi204N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi347N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi535N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi550N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

Cleaved by autocatalysis into a large and a small subunit.By similarity

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

EPDiP36269
PaxDbiP36269
PeptideAtlasiP36269
PRIDEiP36269
ProteomicsDBi55177
55178 [P36269-2]
55179 [P36269-3]

PTM databases

iPTMnetiP36269
PhosphoSitePlusiP36269

Expressioni

Gene expression databases

BgeeiENSG00000099998 Expressed in 154 organ(s), highest expression level in endocervix
CleanExiHS_GGT5
ExpressionAtlasiP36269 baseline and differential
GenevisibleiP36269 HS

Organism-specific databases

HPAiCAB032489
HPA008121

Interactioni

Subunit structurei

Heterodimer composed of the light and heavy chains. The active site is located in the light chain (By similarity).By similarity

Protein-protein interaction databases

BioGridi108954, 5 interactors
STRINGi9606.ENSP00000381340

Structurei

3D structure databases

ProteinModelPortaliP36269
SMRiP36269
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni469 – 470Glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the gamma-glutamyltransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2410 Eukaryota
COG0405 LUCA
GeneTreeiENSGT00550000074591
HOGENOMiHOG000175620
HOVERGENiHBG005835
InParanoidiP36269
KOiK18592
OMAiWRLWDPR
OrthoDBiEOG091G03Y3
PhylomeDBiP36269
TreeFamiTF313608

Family and domain databases

InterProiView protein in InterPro
IPR000101 GGT_peptidase
IPR029055 Ntn_hydrolases_N
PANTHERiPTHR11686 PTHR11686, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00462 G_GLU_TRANSPEPTIDASE, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P36269-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MARGYGATVS LVLLGLGLAL AVIVLAVVLS RHQAPCGPQA FAHAAVAADS
60 70 80 90 100
KVCSDIGRAI LQQQGSPVDA TIAALVCTSV VNPQSMGLGG GVIFTIYNVT
110 120 130 140 150
TGKVEVINAR ETVPASHAPS LLDQCAQALP LGTGAQWIGV PGELRGYAEA
160 170 180 190 200
HRRHGRLPWA QLFQPTIALL RGGHVVAPVL SRFLHNSILR PSLQASTLRQ
210 220 230 240 250
LFFNGTEPLR PQDPLPWPAL ATTLETVATE GVEVFYTGRL GQMLVEDIAK
260 270 280 290 300
EGSQLTLQDL AKFQPEVVDA LEVPLGDYTL YSPPPPAGGA ILSFILNVLR
310 320 330 340 350
GFNFSTESMA RPEGRVNVYH HLVETLKFAK GQRWRLGDPR SHPKLQNASR
360 370 380 390 400
DLLGETLAQL IRQQIDGRGD HQLSHYSLAE AWGHGTGTSH VSVLGEDGSA
410 420 430 440 450
VAATSTINTP FGAMVYSPRT GIILNNELLD LCERCPRGSG TTPSPVSGDR
460 470 480 490 500
VGGAPGRCWP PVPGERSPSS MVPSILINKA QGSKLVIGGA GGELIISAVA
510 520 530 540 550
QAIMSKLWLG FDLRAAIAAP ILHVNSKGCV EYEPNFSQEV QRGLQDRGQN
560 570 580
QTQRPFFLNV VQAVSQEGAC VYAVSDLRKS GEAAGY
Length:586
Mass (Da):62,261
Last modified:October 17, 2006 - v2
Checksum:iEE37B3CE516F5788
GO
Isoform 2 (identifier: P36269-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-132: Missing.

Note: No experimental confirmation available.
Show »
Length:554
Mass (Da):58,992
Checksum:i86001882BE5A0D10
GO
Isoform 3 (identifier: P36269-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     445-445: P → PA

Note: No experimental confirmation available.
Show »
Length:587
Mass (Da):62,332
Checksum:iC7F2285F9FFF1A22
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C1X2H7C1X2_HUMAN
Glutathione hydrolase 5 proenzyme
GGT5
220Annotation score:
C9JU68C9JU68_HUMAN
Glutathione hydrolase 5 proenzyme
GGT5
73Annotation score:

Sequence cautioni

The sequence CAB55910 differs from that shown. Reason: Frameshift at position 446.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti408N → Y in CAB55910 (PubMed:17974005).Curated1
Sequence conflicti437R → W in AAA58503 (PubMed:1676842).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02800611L → I. Corresponds to variant dbSNP:rs5760274Ensembl.1
Natural variantiVAR_028007330K → R2 PublicationsCorresponds to variant dbSNP:rs2275984Ensembl.1
Natural variantiVAR_028008332Q → H. Corresponds to variant dbSNP:rs6004105Ensembl.1
Natural variantiVAR_024455475I → V. Corresponds to variant dbSNP:rs7288201Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008146101 – 132Missing in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_043470445P → PA in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64099 mRNA Translation: AAA58503.1
AL117414 mRNA Translation: CAB55910.1 Frameshift.
BT009808 mRNA Translation: AAP88810.1
CT841518 mRNA Translation: CAJ86448.1
AK292006 mRNA Translation: BAF84695.1
AP000353 Genomic DNA No translation available.
AP000354 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW59643.1
BC011362 mRNA Translation: AAH11362.1
BC073999 mRNA Translation: AAH73999.1
CCDSiCCDS13825.1 [P36269-1]
CCDS42989.1 [P36269-2]
CCDS42990.1 [P36269-3]
PIRiA41125
T17220
RefSeqiNP_001093251.1, NM_001099781.2 [P36269-3]
NP_001093252.1, NM_001099782.2 [P36269-2]
NP_001289393.1, NM_001302464.1
NP_001289394.1, NM_001302465.1
NP_004112.2, NM_004121.3 [P36269-1]
UniGeneiHs.437156

Genome annotation databases

EnsembliENST00000263112; ENSP00000263112; ENSG00000099998 [P36269-2]
ENST00000327365; ENSP00000330080; ENSG00000099998 [P36269-1]
ENST00000398292; ENSP00000381340; ENSG00000099998 [P36269-3]
GeneIDi2687
KEGGihsa:2687
UCSCiuc002zzo.4 human [P36269-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64099 mRNA Translation: AAA58503.1
AL117414 mRNA Translation: CAB55910.1 Frameshift.
BT009808 mRNA Translation: AAP88810.1
CT841518 mRNA Translation: CAJ86448.1
AK292006 mRNA Translation: BAF84695.1
AP000353 Genomic DNA No translation available.
AP000354 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW59643.1
BC011362 mRNA Translation: AAH11362.1
BC073999 mRNA Translation: AAH73999.1
CCDSiCCDS13825.1 [P36269-1]
CCDS42989.1 [P36269-2]
CCDS42990.1 [P36269-3]
PIRiA41125
T17220
RefSeqiNP_001093251.1, NM_001099781.2 [P36269-3]
NP_001093252.1, NM_001099782.2 [P36269-2]
NP_001289393.1, NM_001302464.1
NP_001289394.1, NM_001302465.1
NP_004112.2, NM_004121.3 [P36269-1]
UniGeneiHs.437156

3D structure databases

ProteinModelPortaliP36269
SMRiP36269
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108954, 5 interactors
STRINGi9606.ENSP00000381340

Chemistry databases

SwissLipidsiSLP:000001455

Protein family/group databases

MEROPSiT03.002

PTM databases

iPTMnetiP36269
PhosphoSitePlusiP36269

Polymorphism and mutation databases

BioMutaiGGT5
DMDMi116242493

Proteomic databases

EPDiP36269
PaxDbiP36269
PeptideAtlasiP36269
PRIDEiP36269
ProteomicsDBi55177
55178 [P36269-2]
55179 [P36269-3]

Protocols and materials databases

DNASUi2687
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263112; ENSP00000263112; ENSG00000099998 [P36269-2]
ENST00000327365; ENSP00000330080; ENSG00000099998 [P36269-1]
ENST00000398292; ENSP00000381340; ENSG00000099998 [P36269-3]
GeneIDi2687
KEGGihsa:2687
UCSCiuc002zzo.4 human [P36269-1]

Organism-specific databases

CTDi2687
DisGeNETi2687
EuPathDBiHostDB:ENSG00000099998.17
GeneCardsiGGT5
H-InvDBiHIX0138942
HGNCiHGNC:4260 GGT5
HPAiCAB032489
HPA008121
MIMi137168 gene
neXtProtiNX_P36269
OpenTargetsiENSG00000099998
PharmGKBiPA162389442
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2410 Eukaryota
COG0405 LUCA
GeneTreeiENSGT00550000074591
HOGENOMiHOG000175620
HOVERGENiHBG005835
InParanoidiP36269
KOiK18592
OMAiWRLWDPR
OrthoDBiEOG091G03Y3
PhylomeDBiP36269
TreeFamiTF313608

Enzyme and pathway databases

UniPathwayi
UPA00204

UPA00880

BioCyciMetaCyc:HS01949-MONOMER
BRENDAi2.3.2.2 2681
3.4.19.13 2681
ReactomeiR-HSA-174403 Glutathione synthesis and recycling
R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-HSA-5423646 Aflatoxin activation and detoxification

Miscellaneous databases

GeneWikiiGGTLA1
GenomeRNAii2687
PROiPR:P36269
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000099998 Expressed in 154 organ(s), highest expression level in endocervix
CleanExiHS_GGT5
ExpressionAtlasiP36269 baseline and differential
GenevisibleiP36269 HS

Family and domain databases

InterProiView protein in InterPro
IPR000101 GGT_peptidase
IPR029055 Ntn_hydrolases_N
PANTHERiPTHR11686 PTHR11686, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00462 G_GLU_TRANSPEPTIDASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGGT5_HUMAN
AccessioniPrimary (citable) accession number: P36269
Secondary accession number(s): Q53XM9
, Q6GMP0, Q96FC1, Q9UFM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 17, 2006
Last modified: November 7, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
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