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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Mannheimia haemolytica (Pasteurella haemolytica)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Caution

Was originally thought to have endopeptidase activity (PubMed:1885539). But it could not be confirmed with orthologs purified from P.abyssi (PubMed:17766251, PubMed:21183954).1 Publication

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111IronUniRule annotation1
Metal bindingi115IronUniRule annotation1
Binding sitei167SubstrateUniRule annotation1
Binding sitei180Substrate; via amide nitrogenUniRule annotation1
Binding sitei277SubstrateUniRule annotation1
Metal bindingi305IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
OrganismiMannheimia haemolytica (Pasteurella haemolytica)
Taxonomic identifieri75985 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeMannheimia

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969761 – 325tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST325

Structurei

3D structure databases

ProteinModelPortaliP36175
SMRiP36175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 138Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM Bacteria
COG0533 LUCA

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR017860 Peptidase_M22_CS
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

P36175-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRILGIETSC DETGVAIYDE DKGLVANQLY SQIDMHADYG GVVPELASRD
60 70 80 90 100
HIRKTLPLIQ EALKEANLQP SDIDGIAYTA GPGLVGALLV GSTIARSLAY
110 120 130 140 150
AWNVPALGVH HMEGHLLAPM LEENAPEFPF VALLISGGHT QLVKVDGVGQ
160 170 180 190 200
YELLGESIDD AAGEAFDKTG KLLGLDYPAG VAMSKLAESG TPNRFKFPRP
210 220 230 240 250
MTDRPGLDFS FSGLKTFAAN TIKANLNENG ELDEQTKCDI AHAFQQAVVD
260 270 280 290 300
TILIKCKRAL EQTGYKRLVM AGGVSANKQL RADLAEMMKK LKGEVFYPRP
310 320
QFCTDNGAMI AYTGFLRLKT MNKPT
Length:325
Mass (Da):35,190
Last modified:June 1, 1994 - v1
Checksum:i161D58F60AD1D589
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15958 Genomic DNA Translation: AAA80282.1
PIRiA38108

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15958 Genomic DNA Translation: AAA80282.1
PIRiA38108

3D structure databases

ProteinModelPortaliP36175
SMRiP36175
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CPM Bacteria
COG0533 LUCA

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR017860 Peptidase_M22_CS
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTSAD_MANHA
AccessioniPrimary (citable) accession number: P36175
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 10, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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