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UniProtKB - P36156 (GTO2_YEAST)
Protein
Glutathione S-transferase omega-like 2
Gene
ECM4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). May be involved in cell wall organization and biogenesis.
1 PublicationMiscellaneous
Present with 1670 molecules/cell in log phase SD medium.1 Publication
A version of this protein truncated after amino acid 200 is not active in the beta-hydroxyethyl disulfide (HED) assay.
Catalytic activityi
Kineticsi
- KM=1.42 mM for reduced glutathione (GSH)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 46 | Nucleophile1 Publication | 1 |
GO - Molecular functioni
- glutathione dehydrogenase (ascorbate) activity Source: UniProtKB-EC
- glutathione transferase activity Source: SGD
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- glutathione metabolic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase, Transferase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BRENDAi | 1.8.5.7, 984 |
SABIO-RKi | P36156 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ECM4 Synonyms:GTO2 Ordered Locus Names:YKR076W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001784, ECM4 |
VEuPathDBi | FungiDB:YKR076W |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 46 | C → S or Y: Completely inactive as thiol transferase. No activity recovered against 1-chloro-2,4-dinitrobenzene (CDNB). 1 Publication | 1 | |
Mutagenesisi | 51 | R → A: No effect on thiol transferase activity. No effect on thiol transferase activity; when associated with D-173. 1 Publication | 1 | |
Mutagenesisi | 173 | E → A or D: No effect on thiol transferase activity. 1 Publication | 1 | |
Mutagenesisi | 174 | S → A: No effect on thiol transferase activity. 1 Publication | 1 | |
Mutagenesisi | 246 | L → A: No effect on thiol transferase activity. 1 Publication | 1 | |
Mutagenesisi | 280 | G → L: No effect on thiol transferase activity. 1 Publication | 1 | |
Mutagenesisi | 287 | D → G: Abolishes thiol transferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086922 | 1 – 370 | Glutathione S-transferase omega-like 2Add BLAST | 370 |
Proteomic databases
MaxQBi | P36156 |
PaxDbi | P36156 |
PRIDEi | P36156 |
Expressioni
Inductioni
Under oxidative stress conditions. By agents such as diamide, 1-chloro-2,4-dinitrobenzene, tert-butyl hydroperoxide (t-BOOH) and cadmium in a transcriptional factors YAP1 and/or MSN2/4-dependent manner.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 34207, 264 interactors |
STRINGi | 4932.YKR076W |
Miscellaneous databases
RNActi | P36156, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P36156 |
SMRi | P36156 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 201 – 353 | GST C-terminalAdd BLAST | 153 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2903, Eukaryota |
GeneTreei | ENSGT00530000065151 |
HOGENOMi | CLU_037263_0_1_1 |
InParanoidi | P36156 |
OMAi | PWANRAI |
Family and domain databases
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR016639, GST_Omega/GSH IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR32419, PTHR32419, 1 hit |
Pfami | View protein in Pfam PF13409, GST_N_2, 1 hit |
PIRSFi | PIRSF015753, GST, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P36156-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKQWASGTN GAFKRQVSSF RETISKQHPI YKPAKGRYWL YVSLACPWAH
60 70 80 90 100
RTLITRALKG LTSVIGCSVV HWHLDEKGWR FLDMEKQLED SEDFLEHWHD
110 120 130 140 150
VAGGIRTAKE DSSKSFAEIK NDSQRFMVDA TNEPHYGYKR ISDLYYKSDP
160 170 180 190 200
QYSARFTVPV LWDLETQTIV NNESSEIIRI LNSSAFDEFV DDDHKKTDLV
210 220 230 240 250
PAQLKTQIDD FNSWVYDSIN NGVYKTGFAE KAEVYESEVN NVFEHLDKVE
260 270 280 290 300
KILSDKYSKL KAKYGEEDRQ KILGEFFTVG DQLTEADIRL YTTVIRFDPV
310 320 330 340 350
YVQHFKCNFT SIRAGYPFIH LWVRNLYWNY DAFRYTTDFD HIKLHYTRSH
360 370
TRINPLGITP LGPKPDIRPL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28301 Genomic DNA Translation: CAA82155.1 BK006944 Genomic DNA Translation: DAA09226.1 |
PIRi | S38153 |
RefSeqi | NP_013002.3, NM_001179866.3 |
Genome annotation databases
EnsemblFungii | YKR076W_mRNA; YKR076W; YKR076W |
GeneIDi | 853951 |
KEGGi | sce:YKR076W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28301 Genomic DNA Translation: CAA82155.1 BK006944 Genomic DNA Translation: DAA09226.1 |
PIRi | S38153 |
RefSeqi | NP_013002.3, NM_001179866.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5LKB | X-ray | 1.45 | A/B | 1-370 | [»] | |
5LKD | X-ray | 1.68 | A/B | 1-370 | [»] | |
AlphaFoldDBi | P36156 | |||||
SMRi | P36156 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34207, 264 interactors |
STRINGi | 4932.YKR076W |
Proteomic databases
MaxQBi | P36156 |
PaxDbi | P36156 |
PRIDEi | P36156 |
Genome annotation databases
EnsemblFungii | YKR076W_mRNA; YKR076W; YKR076W |
GeneIDi | 853951 |
KEGGi | sce:YKR076W |
Organism-specific databases
SGDi | S000001784, ECM4 |
VEuPathDBi | FungiDB:YKR076W |
Phylogenomic databases
eggNOGi | KOG2903, Eukaryota |
GeneTreei | ENSGT00530000065151 |
HOGENOMi | CLU_037263_0_1_1 |
InParanoidi | P36156 |
OMAi | PWANRAI |
Enzyme and pathway databases
BRENDAi | 1.8.5.7, 984 |
SABIO-RKi | P36156 |
Miscellaneous databases
PROi | PR:P36156 |
RNActi | P36156, protein |
Family and domain databases
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR016639, GST_Omega/GSH IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR32419, PTHR32419, 1 hit |
Pfami | View protein in Pfam PF13409, GST_N_2, 1 hit |
PIRSFi | PIRSF015753, GST, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GTO2_YEAST | |
Accessioni | P36156Primary (citable) accession number: P36156 Secondary accession number(s): D6VXD6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families