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Entry version 171 (02 Dec 2020)
Sequence version 1 (01 Jun 1994)
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Protein

Transcription initiation factor IIE subunit alpha

Gene

TFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase (By similarity).By similarity

Miscellaneous

Present with 35900 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri124 – 152C4-typeSequence analysisAdd BLAST29

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-674695, RNA Polymerase II Pre-transcription Events
R-SCE-6807505, RNA polymerase II transcribes snRNA genes
R-SCE-73776, RNA Polymerase II Promoter Escape
R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953, RNA Polymerase II Transcription Initiation
R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcription initiation factor IIE subunit alpha
Short name:
TFIIE-alpha
Alternative name(s):
Factor A 66 kDa subunit
Transcription factor A large subunit
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TFA1
Ordered Locus Names:YKL028W
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XI

Organism-specific databases

Eukaryotic Pathogen and Host Database Resources

More...
EuPathDBi
FungiDB:YKL028W

Saccharomyces Genome Database

More...
SGDi
S000001511, TFA1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002112251 – 482Transcription initiation factor IIE subunit alphaAdd BLAST482

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P36100

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P36100

PRoteomics IDEntifications database

More...
PRIDEi
P36100

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

TFIIE is a tetramer of two alpha (TFA1) and two beta (TFA2) subunits.

By similarity

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
34103, 171 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-1658, General transcription factor complex TFIIE

Database of interacting proteins

More...
DIPi
DIP-1700N

Protein interaction database and analysis system

More...
IntActi
P36100, 7 interactors

Molecular INTeraction database

More...
MINTi
P36100

STRING: functional protein association networks

More...
STRINGi
4932.YKL028W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P36100, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P36100

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini9 – 99HTH TFE/IIEalpha-typePROSITE-ProRule annotationAdd BLAST91

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi374 – 482Asp/Glu-rich (highly acidic)Add BLAST109
Compositional biasi374 – 392Poly-GluAdd BLAST19

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TFIIE alpha subunit family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri124 – 152C4-typeSequence analysisAdd BLAST29

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2593, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000016696

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_035744_2_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P36100

Identification of Orthologs from Complete Genome Data

More...
OMAi
HHREAWT

Family and domain databases

Database of protein disorder

More...
DisProti
DP01237

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.40.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR039997, TFE
IPR017919, TFIIE/TFIIEa_HTH
IPR002853, TFIIE_asu
IPR024550, TFIIEa/SarR/Rpc3_HTH_dom
IPR013083, Znf_RING/FYVE/PHD
IPR013137, Znf_TFIIB

The PANTHER Classification System

More...
PANTHERi
PTHR13097, PTHR13097, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08271, TF_Zn_Ribbon, 1 hit
PF02002, TFIIE_alpha, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00531, TFIIE, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51344, HTH_TFE_IIE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P36100-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN
60 70 80 90 100
KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW
110 120 130 140 150
KVHQVVQRLK DDLDKNSEPN GYMCPICLTK YTQLEAVQLL NFDRTEFLCS
160 170 180 190 200
LCDEPLVEDD SGKKNKEKQD KLNRLMDQIQ PIIDSLKKID DSRIEENTFE
210 220 230 240 250
IALARLIPPQ NQSHAAYTYN PKKGSTMFRP GDSAPLPNLM GTALGNDSSR
260 270 280 290 300
RAGANSQATL HINITTASDE VAQRELQERQ AEEKRKQNAV PEWHKQSTIG
310 320 330 340 350
KTALGRLDNE EEFDPVVTAS AMDSINPDNE PAQETSYQNN RTLTEQEMEE
360 370 380 390 400
RENEKTLNDY YAALAKKQAK LNKEEEEEEE EEEDEEEEEE EEMEDVMDDN
410 420 430 440 450
DETARENALE DEFEDVTDTA GTAKTESNTS NDVKQESIND KTEDAVNATA
460 470 480
TASGPSANAK PNDGDDDDDD DDDEMDIEFE DV
Length:482
Mass (Da):54,742
Last modified:June 1, 1994 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3E789DFC4247EF8A
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U12825 Genomic DNA Translation: AAA62665.1
Z28028 Genomic DNA Translation: CAA81863.1
BK006944 Genomic DNA Translation: DAA09126.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S37845

NCBI Reference Sequences

More...
RefSeqi
NP_012897.3, NM_001179594.3

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YKL028W_mRNA; YKL028W; YKL028W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
853840

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YKL028W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12825 Genomic DNA Translation: AAA62665.1
Z28028 Genomic DNA Translation: CAA81863.1
BK006944 Genomic DNA Translation: DAA09126.1
PIRiS37845
RefSeqiNP_012897.3, NM_001179594.3

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FMFelectron microscopy6.00R1-160[»]
5FYWelectron microscopy4.35W1-482[»]
5FZ5electron microscopy8.80W1-482[»]
5OQJelectron microscopy4.70W1-482[»]
5OQMelectron microscopy5.80W332-482[»]
5SVAelectron microscopy15.30h1-482[»]
6GYLelectron microscopy4.80W1-482[»]
6GYMelectron microscopy6.70W1-482[»]
SMRiP36100
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi34103, 171 interactors
ComplexPortaliCPX-1658, General transcription factor complex TFIIE
DIPiDIP-1700N
IntActiP36100, 7 interactors
MINTiP36100
STRINGi4932.YKL028W

Proteomic databases

MaxQBiP36100
PaxDbiP36100
PRIDEiP36100

Genome annotation databases

EnsemblFungiiYKL028W_mRNA; YKL028W; YKL028W
GeneIDi853840
KEGGisce:YKL028W

Organism-specific databases

EuPathDBiFungiDB:YKL028W
SGDiS000001511, TFA1

Phylogenomic databases

eggNOGiKOG2593, Eukaryota
GeneTreeiENSGT00390000016696
HOGENOMiCLU_035744_2_0_1
InParanoidiP36100
OMAiHHREAWT

Enzyme and pathway databases

ReactomeiR-SCE-674695, RNA Polymerase II Pre-transcription Events
R-SCE-6807505, RNA polymerase II transcribes snRNA genes
R-SCE-73776, RNA Polymerase II Promoter Escape
R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953, RNA Polymerase II Transcription Initiation
R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P36100
RNActiP36100, protein

Family and domain databases

DisProtiDP01237
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039997, TFE
IPR017919, TFIIE/TFIIEa_HTH
IPR002853, TFIIE_asu
IPR024550, TFIIEa/SarR/Rpc3_HTH_dom
IPR013083, Znf_RING/FYVE/PHD
IPR013137, Znf_TFIIB
PANTHERiPTHR13097, PTHR13097, 1 hit
PfamiView protein in Pfam
PF08271, TF_Zn_Ribbon, 1 hit
PF02002, TFIIE_alpha, 1 hit
SMARTiView protein in SMART
SM00531, TFIIE, 1 hit
PROSITEiView protein in PROSITE
PS51344, HTH_TFE_IIE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiT2EA_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P36100
Secondary accession number(s): D6VXQ6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: December 2, 2020
This is version 171 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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