UniProtKB - P36095 (VPS24_YEAST)
Vacuolar protein-sorting-associated protein 24
VPS24
Functioni
Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment oflate-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. The DID4/VPS2-VPS24 subcomplex is required for the VPS4-dependent dissociation of ESCRT-III.
3 PublicationsMiscellaneous
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- ATP export Source: SGD
- endosome transport via multivesicular body sorting pathway Source: GO_Central
- intralumenal vesicle formation Source: SGD
- late endosome to vacuole transport Source: SGD
- protein transport Source: GO_Central
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: ComplexPortal
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-1632852, Macroautophagy R-SCE-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-SCE-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein-sorting-associated protein 24Alternative name(s): DOA4-independent degradation protein 3 ESCRT-III complex subunit VPS24 |
Gene namesi | Name:VPS24 Synonyms:DID3 Ordered Locus Names:YKL041W ORF Names:YKL254 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001524, VPS24 |
VEuPathDBi | FungiDB:YKL041W |
Subcellular locationi
Endosome
Other locations
Note: Endosomal and other punctate structures.
Cytosol
- cytosol Source: SGD
Endosome
- ESCRT III complex Source: SGD
- multivesicular body Source: GO_Central
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211487 | 1 – 224 | Vacuolar protein-sorting-associated protein 24Add BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 203 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P36095 |
PaxDbi | P36095 |
PRIDEi | P36095 |
PTM databases
iPTMneti | P36095 |
Interactioni
Subunit structurei
Core component of the ESCRT-III complex (endosomal sorting required for transport complex III). ESCRT-III appears to be sequentially assembled as a flat lattice on the endosome membrane and forms a transient 450 kDa complex that contains DID4, oligomerized SNF7, VPS20 and VPS24. SNF7 oligomerization into a membrane-associated filament is nucleated by association of SNF7 with VPS20; the process is terminated through association of VPS24, possibly by capping the SNF7 filament. VPS24 subsequently associates with DID4/VPS2.
Interacts with the VPS4.
Interacts with DID2.
2 PublicationsBinary interactionsi
P36095
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-26653,EBI-26653 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 34091, 709 interactors |
ComplexPortali | CPX-1624, ESCRT-III complex |
DIPi | DIP-7295N |
IntActi | P36095, 6 interactors |
MINTi | P36095 |
STRINGi | 4932.YKL041W |
Miscellaneous databases
RNActi | P36095, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P36095 |
SMRi | P36095 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3229, Eukaryota |
GeneTreei | ENSGT00950000182832 |
HOGENOMi | CLU_069208_0_2_1 |
InParanoidi | P36095 |
OMAi | INEMMDD |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
PANTHERi | PTHR10476, PTHR10476, 1 hit |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDYIKKAIWG PDPKEQQRRI RSVLRKNGRN IEKSLRELTV LQNKTQQLIK
60 70 80 90 100
KSAKKNDVRT VRLYAKELYQ INKQYDRMYT SRAQLDSVRM KIDEAIRMNT
110 120 130 140 150
LSNQMADSAG LMREVNSLVR LPQLRNTMIE LEKELMKSGI ISEMVDDTME
160 170 180 190 200
SVGDVGEEMD EAVDEEVNKI VEQYTNEKFK NVDQVPTVEL AANEEEQEIP
210 220
DEKVDEEADR MVNEMRERLR ALQN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71621 Genomic DNA Translation: CAA50631.1 Z28041 Genomic DNA Translation: CAA81876.1 AY557901 Genomic DNA Translation: AAS56227.1 BK006944 Genomic DNA Translation: DAA09115.1 |
PIRi | S37862 |
RefSeqi | NP_012883.1, NM_001179607.1 |
Genome annotation databases
EnsemblFungii | YKL041W_mRNA; YKL041W; YKL041W |
GeneIDi | 853825 |
KEGGi | sce:YKL041W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71621 Genomic DNA Translation: CAA50631.1 Z28041 Genomic DNA Translation: CAA81876.1 AY557901 Genomic DNA Translation: AAS56227.1 BK006944 Genomic DNA Translation: DAA09115.1 |
PIRi | S37862 |
RefSeqi | NP_012883.1, NM_001179607.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6ZH3 | electron microscopy | 3.20 | A/B/C/D | 1-224 | [»] | |
AlphaFoldDBi | P36095 | |||||
SMRi | P36095 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34091, 709 interactors |
ComplexPortali | CPX-1624, ESCRT-III complex |
DIPi | DIP-7295N |
IntActi | P36095, 6 interactors |
MINTi | P36095 |
STRINGi | 4932.YKL041W |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
PTM databases
iPTMneti | P36095 |
Proteomic databases
MaxQBi | P36095 |
PaxDbi | P36095 |
PRIDEi | P36095 |
Genome annotation databases
EnsemblFungii | YKL041W_mRNA; YKL041W; YKL041W |
GeneIDi | 853825 |
KEGGi | sce:YKL041W |
Organism-specific databases
SGDi | S000001524, VPS24 |
VEuPathDBi | FungiDB:YKL041W |
Phylogenomic databases
eggNOGi | KOG3229, Eukaryota |
GeneTreei | ENSGT00950000182832 |
HOGENOMi | CLU_069208_0_2_1 |
InParanoidi | P36095 |
OMAi | INEMMDD |
Enzyme and pathway databases
Reactomei | R-SCE-1632852, Macroautophagy R-SCE-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-SCE-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III |
Miscellaneous databases
PROi | PR:P36095 |
RNActi | P36095, protein |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
PANTHERi | PTHR10476, PTHR10476, 1 hit |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS24_YEAST | |
Accessioni | P36095Primary (citable) accession number: P36095 Secondary accession number(s): D6VXP5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families