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UniProtKB - P36091 (DCW1_YEAST)
Protein
Mannan endo-1,6-alpha-mannosidase DCW1
Gene
DCW1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for normal synthesis of the cell wall.
1 PublicationMiscellaneous
Present with 2580 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans. EC:3.2.1.101
GO - Molecular functioni
- mannan endo-1,6-alpha-mannosidase activity Source: UniProtKB-EC
GO - Biological processi
- budding cell bud growth Source: SGD
- carbohydrate catabolic process Source: InterPro
- cell wall organization Source: UniProtKB-KW
- fungal-type cell wall biogenesis Source: SGD
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Protein family/group databases
CAZyi | GH76, Glycoside Hydrolase Family 76 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannan endo-1,6-alpha-mannosidase DCW1 (EC:3.2.1.101)Alternative name(s): Defective cell wall 1 Endo-alpha-1->6-D-mannanase DCW1 |
Gene namesi | Name:DCW1 Ordered Locus Names:YKL046C ORF Names:YKL259 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001529, DCW1 |
VEuPathDBi | FungiDB:YKL046C |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; GPI-anchor 1 Publication
Note: GPI-anchored plasma membrane protein (GPI-PMP).
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Plasma Membrane
- anchored component of plasma membrane Source: SGD
Other locations
- cellular bud Source: SGD
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000012125 | 22 – 428 | Mannan endo-1,6-alpha-mannosidase DCW1Add BLAST | 407 | |
PropeptideiPRO_0000012126 | 429 – 449 | Removed in mature formSequence analysisAdd BLAST | 21 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 34 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 84 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 109 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 203 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 225 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 240 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 265 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 281 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 337 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 362 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 420 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 428 | GPI-anchor amidated glycineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PaxDbi | P36091 |
PRIDEi | P36091 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34087, 91 interactors |
IntActi | P36091, 2 interactors |
MINTi | P36091 |
STRINGi | 4932.YKL046C |
Miscellaneous databases
RNActi | P36091, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 76 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QSWP, Eukaryota |
HOGENOMi | CLU_025694_1_2_1 |
InParanoidi | P36091 |
OMAi | TACGFKW |
Family and domain databases
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR005198, Glyco_hydro_76 IPR014480, Mannan-1_6-alpha_mannosidase |
PANTHERi | PTHR12145, PTHR12145, 1 hit |
Pfami | View protein in Pfam PF03663, Glyco_hydro_76, 1 hit |
PIRSFi | PIRSF016302, Man_a_manosd, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P36091-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN
60 70 80 90 100
QYGKTVGMFS DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG
110 120 130 140 150
DDNDYIPLNQ STTEGNDDQA FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA
160 170 180 190 200
VFNTMALRWD ADSCGGGLRW QIFVWNSGYD YKNTVSNGAL FHIAARLARY
210 220 230 240 250
TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN CTKVTSYQWT
260 270 280 290 300
YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP
310 320 330 340 350
NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG
360 370 380 390 400
TDGHTCGLNW FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS
410 420 430 440
SVGDGAAGTQ AQPTNLAPLN ITKGSKAGAG IITAVIGISI VACALWLVF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 276 | T → A in AAU09756 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71621 Genomic DNA No translation available. Z28046 Genomic DNA Translation: CAA81881.1 AY723839 Genomic DNA Translation: AAU09756.1 BK006944 Genomic DNA Translation: DAA09111.1 |
PIRi | S37867 |
RefSeqi | NP_012878.1, NM_001179612.1 |
Genome annotation databases
EnsemblFungii | YKL046C_mRNA; YKL046C; YKL046C |
GeneIDi | 853820 |
KEGGi | sce:YKL046C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71621 Genomic DNA No translation available. Z28046 Genomic DNA Translation: CAA81881.1 AY723839 Genomic DNA Translation: AAU09756.1 BK006944 Genomic DNA Translation: DAA09111.1 |
PIRi | S37867 |
RefSeqi | NP_012878.1, NM_001179612.1 |
3D structure databases
SMRi | P36091 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34087, 91 interactors |
IntActi | P36091, 2 interactors |
MINTi | P36091 |
STRINGi | 4932.YKL046C |
Protein family/group databases
CAZyi | GH76, Glycoside Hydrolase Family 76 |
Proteomic databases
PaxDbi | P36091 |
PRIDEi | P36091 |
Genome annotation databases
EnsemblFungii | YKL046C_mRNA; YKL046C; YKL046C |
GeneIDi | 853820 |
KEGGi | sce:YKL046C |
Organism-specific databases
SGDi | S000001529, DCW1 |
VEuPathDBi | FungiDB:YKL046C |
Phylogenomic databases
eggNOGi | ENOG502QSWP, Eukaryota |
HOGENOMi | CLU_025694_1_2_1 |
InParanoidi | P36091 |
OMAi | TACGFKW |
Miscellaneous databases
PROi | PR:P36091 |
RNActi | P36091, protein |
Family and domain databases
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR005198, Glyco_hydro_76 IPR014480, Mannan-1_6-alpha_mannosidase |
PANTHERi | PTHR12145, PTHR12145, 1 hit |
Pfami | View protein in Pfam PF03663, Glyco_hydro_76, 1 hit |
PIRSFi | PIRSF016302, Man_a_manosd, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCW1_YEAST | |
Accessioni | P36091Primary (citable) accession number: P36091 Secondary accession number(s): D6VXP1, Q66R60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | September 29, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families