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UniProtKB - P36059 (NNRD_YEAST)
Protein
ATP-dependent (S)-NAD(P)H-hydrate dehydratase
Gene
NNR2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
UniRule annotation1 PublicationMiscellaneous
Present with 3870 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Cofactori
Mg2+UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 121 | NADPHX; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 250 | NADPHXUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 218 – 222 | ATPUniRule annotation | 5 | |
Nucleotide bindingi | 240 – 249 | ATPUniRule annotation | 10 |
GO - Molecular functioni
- ADP-dependent NAD(P)H-hydrate dehydratase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- ATP-dependent NAD(P)H-hydrate dehydratase activity Source: SGD
GO - Biological processi
- metabolite repair Source: GO_Central
- nicotinamide nucleotide metabolic process Source: SGD
Keywordsi
Molecular function | Lyase |
Ligand | ATP-binding, NAD, NADP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 4.2.1.93, 984 |
Reactomei | R-SCE-197264, Nicotinamide salvaging |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation)Alternative name(s): ATP-dependent NAD(P)HX dehydrataseUniRule annotation Nicotinamide nucleotide repair protein 21 Publication |
Gene namesi | Name:NNR21 Publication Ordered Locus Names:YKL151C ORF Names:YKL606 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001634, NNR2 |
VEuPathDBi | FungiDB:YKL151C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119053 | 1 – 337 | ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST | 337 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P36059 |
PaxDbi | P36059 |
PRIDEi | P36059 |
PTM databases
iPTMneti | P36059 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 33986, 50 interactors |
IntActi | P36059, 2 interactors |
MINTi | P36059 |
STRINGi | 4932.YKL151C |
Miscellaneous databases
RNActi | P36059, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 335 | YjeF C-terminalUniRule annotationAdd BLAST | 325 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 182 – 188 | NADPHXUniRule annotation | 7 | |
Regioni | 224 – 246 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 224 – 238 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the NnrD/CARKD family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3974, Eukaryota |
GeneTreei | ENSGT00390000000917 |
HOGENOMi | CLU_030651_0_0_1 |
InParanoidi | P36059 |
OMAi | HIPHTNE |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P36059-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAELSHREL IKLAQKRCIP PLLPKFHKGQ SGGRVCIIGG CEDYTGAPYF
60 70 80 90 100
SANATALMGC DLTHVICEYN AGTVIKSYTP NLMVHPYLRM SNTKLDVDMD
110 120 130 140 150
EQRKKINSLL DRIHVVVIGP GLGRDPLMLK SIKDIIRYIL EKHEGKIPLV
160 170 180 190 200
IDADGLFLVT QDSEVKEMLK SYPKGRVILT PNVVEFKRLC DAIGKKGDSH
210 220 230 240 250
SEMGSLIAQE LNCIVVEKGQ SDKIFSPDSE KDMLTNSEEG SNKRVGGQGD
260 270 280 290 300
TLTGAISCML AFSRAMYDFK ICEQEEKGES SNDKPLKNWV DYAMLSCYAG
310 320 330
CTITRECSRL GFKAKGRAMQ TTDLNDRVGE VFAKLFG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 182 | N → D in AAS56241 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26877 Genomic DNA Translation: CAA81502.1 Z28151 Genomic DNA Translation: CAA81992.1 AY557915 Genomic DNA Translation: AAS56241.1 BK006944 Genomic DNA Translation: DAA09012.1 |
PIRi | S37799 |
RefSeqi | NP_012771.1, NM_001179717.1 |
Genome annotation databases
EnsemblFungii | YKL151C_mRNA; YKL151C; YKL151C |
GeneIDi | 853706 |
KEGGi | sce:YKL151C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26877 Genomic DNA Translation: CAA81502.1 Z28151 Genomic DNA Translation: CAA81992.1 AY557915 Genomic DNA Translation: AAS56241.1 BK006944 Genomic DNA Translation: DAA09012.1 |
PIRi | S37799 |
RefSeqi | NP_012771.1, NM_001179717.1 |
3D structure databases
AlphaFoldDBi | P36059 |
SMRi | P36059 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33986, 50 interactors |
IntActi | P36059, 2 interactors |
MINTi | P36059 |
STRINGi | 4932.YKL151C |
PTM databases
iPTMneti | P36059 |
Proteomic databases
MaxQBi | P36059 |
PaxDbi | P36059 |
PRIDEi | P36059 |
Genome annotation databases
EnsemblFungii | YKL151C_mRNA; YKL151C; YKL151C |
GeneIDi | 853706 |
KEGGi | sce:YKL151C |
Organism-specific databases
SGDi | S000001634, NNR2 |
VEuPathDBi | FungiDB:YKL151C |
Phylogenomic databases
eggNOGi | KOG3974, Eukaryota |
GeneTreei | ENSGT00390000000917 |
HOGENOMi | CLU_030651_0_0_1 |
InParanoidi | P36059 |
OMAi | HIPHTNE |
Enzyme and pathway databases
BRENDAi | 4.2.1.93, 984 |
Reactomei | R-SCE-197264, Nicotinamide salvaging |
Miscellaneous databases
PROi | PR:P36059 |
RNActi | P36059, protein |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NNRD_YEAST | |
Accessioni | P36059Primary (citable) accession number: P36059 Secondary accession number(s): D6VX46, Q6Q5N3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - SIMILARITY comments
Index of protein domains and families