UniProtKB - P36048 (SN114_YEAST)
Pre-mRNA-splicing factor SNU114
SNU114
Functioni
Miscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 140 – 147 | GTPBy similarity | 8 | |
Nucleotide bindingi | 214 – 218 | GTPBy similarity | 5 | |
Nucleotide bindingi | 268 – 271 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: SGD
- GTP binding Source: UniProtKB-KW
- U5 snRNA binding Source: SGD
GO - Biological processi
- generation of catalytic spliceosome for first transesterification step Source: SGD
- mRNA splicing, via spliceosome Source: SGD
- spliceosomal tri-snRNP complex assembly Source: SGD
- spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) Source: SGD
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor SNU114Alternative name(s): 114 kDa U5 small nuclear ribonucleoprotein component Growth inhibitory protein 10 |
Gene namesi | Name:SNU114 Synonyms:GIN10 Ordered Locus Names:YKL173W ORF Names:YKL637 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YKL173W |
SGDi | S000001656, SNU114 |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: SGD
- U2-type catalytic step 2 spliceosome Source: GO_Central
- U4/U6 x U5 tri-snRNP complex Source: SGD
- U5 snRNP Source: SGD
Other locations
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091565 | 1 – 1008 | Pre-mRNA-splicing factor SNU114Add BLAST | 1008 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 85 | PhosphoserineCombined sources | 1 | |
Modified residuei | 88 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P36048 |
PaxDbi | P36048 |
PRIDEi | P36048 |
PTM databases
iPTMneti | P36048 |
Interactioni
Subunit structurei
Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
Interacts (via C-terminus) with CWC21.
Interacts (via N-terminus) with PRP8 (via SCwid domain).
4 PublicationsBinary interactionsi
Hide detailsP36048
With | #Exp. | IntAct |
---|---|---|
AAR2 [P32357] | 3 | EBI-243,EBI-340 |
BRR2 [P32639] | 11 | EBI-243,EBI-861 |
Protein-protein interaction databases
BioGRIDi | 33965, 420 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex CPX-25, U4/U6.U5 tri-snRNP complex CPX-29, U5 snRNP CPX-30, AAR2-U5 snRNP |
DIPi | DIP-759N |
IntActi | P36048, 48 interactors |
MINTi | P36048 |
STRINGi | 4932.YKL173W |
Miscellaneous databases
RNActi | P36048, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P36048 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 131 – 338 | tr-type GPROSITE-ProRule annotationAdd BLAST | 208 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 140 – 147 | G1PROSITE-ProRule annotation | 8 | |
Regioni | 188 – 192 | G2PROSITE-ProRule annotation | 5 | |
Regioni | 214 – 217 | G3PROSITE-ProRule annotation | 4 | |
Regioni | 268 – 271 | G4PROSITE-ProRule annotation | 4 | |
Regioni | 315 – 317 | G5PROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0468, Eukaryota |
GeneTreei | ENSGT00940000155685 |
HOGENOMi | CLU_002794_11_2_1 |
InParanoidi | P36048 |
OMAi | GPDEMGP |
Family and domain databases
CDDi | cd04098, eEF2_C_snRNP, 1 hit |
Gene3Di | 3.30.230.10, 1 hit |
InterProi | View protein in InterPro IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031950, EFTUD2_N IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV IPR035655, U5-116kDa_C |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF03764, EFG_IV, 1 hit PF16004, EFTUD2, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
PROSITEi | View protein in PROSITE PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES
60 70 80 90 100
RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL
110 120 130 140 150
QEHTIFTQLK KNIPKTRYNR DYMLSMANIP ERIINVGVIG PLHSGKTSLM
160 170 180 190 200
DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN LKQEIDRGLS IKLNGSTLLC
210 220 230 240 250
TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID VVEGVTFVVE
260 270 280 290 300
QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK
310 320 330 340 350
GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG
360 370 380 390 400
SVYYHKGNFR TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL
410 420 430 440 450
RSNFRVNLSQ EALQYDPQPF LKHVLQLIFR QQTGLVDAIT RCYQPFELFD
460 470 480 490 500
NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG AEWSLVRIYS GLLKRGDTVR
510 520 530 540 550
ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE EIGLLGGRYV
560 570 580 590 600
YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE
610 620 630 640 650
AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL
660 670 680 690 700
YMDCLLYDLR ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE
710 720 730 740 750
ENLPGLSISV AAEPMDSKMI QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI
760 770 780 790 800
LRTEYGWDSL ASRNVWSFYN GNVLINDTLP DEISPELLSK YKEQIIQGFY
810 820 830 840 850
WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL
860 870 880 890 900
LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL
910 920 930 940 950
EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF
960 970 980 990 1000
IPKLKPAPIN SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ
LRENGLVP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26878 Genomic DNA Translation: CAA81514.1 Z28173 Genomic DNA Translation: CAA82015.1 D28149 Genomic DNA Translation: BAA05682.1 BK006944 Genomic DNA Translation: DAA08993.1 |
PIRi | S38003 |
RefSeqi | NP_012748.1, NM_001179739.1 |
Genome annotation databases
EnsemblFungii | YKL173W_mRNA; YKL173W; YKL173W |
GeneIDi | 853681 |
KEGGi | sce:YKL173W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26878 Genomic DNA Translation: CAA81514.1 Z28173 Genomic DNA Translation: CAA82015.1 D28149 Genomic DNA Translation: BAA05682.1 BK006944 Genomic DNA Translation: DAA08993.1 |
PIRi | S38003 |
RefSeqi | NP_012748.1, NM_001179739.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCM | electron microscopy | 3.80 | H | 1-1008 | [»] | |
5GAM | electron microscopy | 3.70 | C | 1-1008 | [»] | |
5GAN | electron microscopy | 3.60 | C | 1-1008 | [»] | |
5GM6 | electron microscopy | 3.50 | C | 1-1008 | [»] | |
5GMK | electron microscopy | 3.40 | C | 1-1008 | [»] | |
5LJ3 | electron microscopy | 3.80 | C | 1-1008 | [»] | |
5LJ5 | electron microscopy | 3.80 | C | 1-1008 | [»] | |
5LQW | electron microscopy | 5.80 | B | 1-1008 | [»] | |
5MPS | electron microscopy | 3.85 | C | 1-1008 | [»] | |
5MQ0 | electron microscopy | 4.17 | C | 1-1008 | [»] | |
5NRL | electron microscopy | 7.20 | C | 1-1008 | [»] | |
5WSG | electron microscopy | 4.00 | C | 1-1008 | [»] | |
5Y88 | electron microscopy | 3.70 | C | 1-1008 | [»] | |
5YLZ | electron microscopy | 3.60 | C | 1-1008 | [»] | |
5ZWM | electron microscopy | 3.40 | C | 1-1008 | [»] | |
5ZWO | electron microscopy | 3.90 | C | 1-1008 | [»] | |
6BK8 | electron microscopy | 3.30 | B | 1-1008 | [»] | |
6EXN | electron microscopy | 3.70 | C | 1-1008 | [»] | |
6J6G | electron microscopy | 3.20 | C | 1-1008 | [»] | |
6J6H | electron microscopy | 3.60 | C | 1-1008 | [»] | |
6J6N | electron microscopy | 3.86 | C | 1-1008 | [»] | |
6J6Q | electron microscopy | 3.70 | C | 1-1008 | [»] | |
6TEO | X-ray | 3.10 | A/C | 72-1008 | [»] | |
SMRi | P36048 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33965, 420 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex CPX-25, U4/U6.U5 tri-snRNP complex CPX-29, U5 snRNP CPX-30, AAR2-U5 snRNP |
DIPi | DIP-759N |
IntActi | P36048, 48 interactors |
MINTi | P36048 |
STRINGi | 4932.YKL173W |
PTM databases
iPTMneti | P36048 |
Proteomic databases
MaxQBi | P36048 |
PaxDbi | P36048 |
PRIDEi | P36048 |
Genome annotation databases
EnsemblFungii | YKL173W_mRNA; YKL173W; YKL173W |
GeneIDi | 853681 |
KEGGi | sce:YKL173W |
Organism-specific databases
EuPathDBi | FungiDB:YKL173W |
SGDi | S000001656, SNU114 |
Phylogenomic databases
eggNOGi | KOG0468, Eukaryota |
GeneTreei | ENSGT00940000155685 |
HOGENOMi | CLU_002794_11_2_1 |
InParanoidi | P36048 |
OMAi | GPDEMGP |
Miscellaneous databases
PROi | PR:P36048 |
RNActi | P36048, protein |
Family and domain databases
CDDi | cd04098, eEF2_C_snRNP, 1 hit |
Gene3Di | 3.30.230.10, 1 hit |
InterProi | View protein in InterPro IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031950, EFTUD2_N IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV IPR035655, U5-116kDa_C |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF03764, EFG_IV, 1 hit PF16004, EFTUD2, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
PROSITEi | View protein in PROSITE PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SN114_YEAST | |
Accessioni | P36048Primary (citable) accession number: P36048 Secondary accession number(s): D6VX27 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 195 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD