UniProtKB - P36046 (MIA40_YEAST)
Mitochondrial intermembrane space import and assembly protein 40
MIA40
Functioni
Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Forms a redox cycle with ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced MIA40 is reoxidized by FAD-linked sulfhydryl oxidase ERV1.
6 PublicationsMiscellaneous
Cofactori
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: SGD
- oxidoreductase activity Source: SGD
- protein disulfide isomerase activity Source: SGD
- protein-disulfide reductase activity Source: GO_Central
- thiol oxidase activity Source: SGD
GO - Biological processi
- protein folding Source: SGD
- protein import into mitochondrial intermembrane space Source: SGD
- protein maturation by protein folding Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Protein transport, Translocation, Transport |
Protein family/group databases
TCDBi | 3.A.8.1.1, the mitochondrial protein translocase (mpt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial intermembrane space import and assembly protein 40Alternative name(s): Mitochondrial import inner membrane translocase TIM40 |
Gene namesi | Name:MIA40 Synonyms:TIM40 Ordered Locus Names:YKL195W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001678, MIA40 |
VEuPathDBi | FungiDB:YKL195W |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 6 Publications; Single-pass type II membrane protein 6 Publications; Intermembrane side 6 Publications
Mitochondrion
- integral component of mitochondrial inner membrane Source: SGD
- mitochondrial intermembrane space Source: SGD
- mitochondrion Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 33 – 46 | Mitochondrial matrixSequence analysisAdd BLAST | 14 | |
Transmembranei | 47 – 66 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 67 – 403 | Mitochondrial intermembraneSequence analysisAdd BLAST | 337 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 296 | C → S: Loss of function; when associated with S-298. 2 Publications | 1 | |
Mutagenesisi | 298 | C → S: Loss of function; when associated with S-296. 2 Publications | 1 | |
Mutagenesisi | 307 | C → S: Loss of function; when associated with S-317. 2 Publications | 1 | |
Mutagenesisi | 317 | C → S: Loss of function; when associated with S-307. 1 Publication | 1 | |
Mutagenesisi | 330 | C → S: Loss of function; when associated with S-340. 1 Publication | 1 | |
Mutagenesisi | 340 | C → S: Loss of function; when associated with S-330. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 31 | Mitochondrion1 PublicationAdd BLAST | 31 | |
ChainiPRO_0000203135 | 32 – 403 | Mitochondrial intermembrane space import and assembly protein 40Add BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 296 ↔ 298 | Redox-active1 Publication | ||
Disulfide bondi | 307 ↔ 340 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 317 ↔ 330 | PROSITE-ProRule annotation1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
MaxQBi | P36046 |
PaxDbi | P36046 |
PRIDEi | P36046 |
PTM databases
iPTMneti | P36046 |
Interactioni
Subunit structurei
Monomer.
Interacts with the FAD-linked sulfhydryl oxidase ERV1 and with the substrate proteins COX17, TIM9, and TIM13, forming transient intermolecular disulfide bridges.
Interacts with FCJ1.
5 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 33926, 214 interactors |
DIPi | DIP-5432N |
IntActi | P36046, 11 interactors |
MINTi | P36046 |
STRINGi | 4932.YKL195W |
Miscellaneous databases
RNActi | P36046, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P36046 |
SMRi | P36046 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P36046 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 304 – 348 | CHCHPROSITE-ProRule annotationAdd BLAST | 45 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 75 – 292 | DisorderedSequence analysisAdd BLAST | 218 | |
Regioni | 351 – 403 | DisorderedSequence analysisAdd BLAST | 53 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 307 – 317 | Cx9C motif 1PROSITE-ProRule annotationAdd BLAST | 11 | |
Motifi | 330 – 340 | Cx9C motif 2PROSITE-ProRule annotationAdd BLAST | 11 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 97 – 118 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 140 – 172 | Basic and acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 202 – 245 | Basic and acidic residuesSequence analysisAdd BLAST | 44 | |
Compositional biasi | 252 – 284 | Basic and acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 351 – 366 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 368 – 383 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 384 – 403 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
Keywords - Domaini
Redox-active center, Signal-anchor, Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4149, Eukaryota |
GeneTreei | ENSGT00940000170392 |
HOGENOMi | CLU_683707_0_0_1 |
InParanoidi | P36046 |
Family and domain databases
InterProi | View protein in InterPro IPR010625, CHCH IPR039289, CHCHD4 |
PANTHERi | PTHR21622, PTHR21622, 1 hit |
Pfami | View protein in Pfam PF06747, CHCH, 1 hit |
PROSITEi | View protein in PROSITE PS51808, CHCH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLRNLVVRNA CRNRPSIQVA RGLCRHQTRR LMASSPQFGR NSNQEKTAGF
60 70 80 90 100
IMGILSMAGA LYFIAPNRKP LFASRKVESD KTAEEELSSG GEQSPENEDD
110 120 130 140 150
NNSKSDENGD DNDSKNDETE AGPQLGGDKI GASKVAEDGE LVVLAEEDNK
160 170 180 190 200
SSEDKDTDES KVSTKDDEQS NEDNATANNQ KDENISSENS EENTSDKTLD
210 220 230 240 250
NNAGSSEKKD PEHSDDEKSQ QGQSDDKTTT EDNNGEEESS KKTVSDSENS
260 270 280 290 300
AKQSESSDEE KEELRKQEEK QMGPTEEEVQ HEGAYNPDTG EINWDCPCLG
310 320 330 340 350
GMAHGPCGEE FKSAFSCFVY SEAEPKGIDC VEKFQHMQDC FRKYPEHYAE
360 370 380 390 400
QLKETSDDEE PQDKVKVNTI ESAPNVSSAK ENAAKKAEQS DVKKEPLNEE
SKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28195 Genomic DNA Translation: CAA82039.1 BK006944 Genomic DNA Translation: DAA08971.1 |
PIRi | S38032 |
RefSeqi | NP_012726.2, NM_001179761.1 |
Genome annotation databases
EnsemblFungii | YKL195W_mRNA; YKL195W; YKL195W |
GeneIDi | 853639 |
KEGGi | sce:YKL195W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28195 Genomic DNA Translation: CAA82039.1 BK006944 Genomic DNA Translation: DAA08971.1 |
PIRi | S38032 |
RefSeqi | NP_012726.2, NM_001179761.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZXT | X-ray | 3.00 | A | 282-365 | [»] | |
3A3C | X-ray | 2.50 | A | 282-353 | [»] | |
AlphaFoldDBi | P36046 | |||||
SMRi | P36046 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33926, 214 interactors |
DIPi | DIP-5432N |
IntActi | P36046, 11 interactors |
MINTi | P36046 |
STRINGi | 4932.YKL195W |
Protein family/group databases
TCDBi | 3.A.8.1.1, the mitochondrial protein translocase (mpt) family |
PTM databases
iPTMneti | P36046 |
Proteomic databases
MaxQBi | P36046 |
PaxDbi | P36046 |
PRIDEi | P36046 |
Genome annotation databases
EnsemblFungii | YKL195W_mRNA; YKL195W; YKL195W |
GeneIDi | 853639 |
KEGGi | sce:YKL195W |
Organism-specific databases
SGDi | S000001678, MIA40 |
VEuPathDBi | FungiDB:YKL195W |
Phylogenomic databases
eggNOGi | KOG4149, Eukaryota |
GeneTreei | ENSGT00940000170392 |
HOGENOMi | CLU_683707_0_0_1 |
InParanoidi | P36046 |
Miscellaneous databases
EvolutionaryTracei | P36046 |
PROi | PR:P36046 |
RNActi | P36046, protein |
Family and domain databases
InterProi | View protein in InterPro IPR010625, CHCH IPR039289, CHCHD4 |
PANTHERi | PTHR21622, PTHR21622, 1 hit |
Pfami | View protein in Pfam PF06747, CHCH, 1 hit |
PROSITEi | View protein in PROSITE PS51808, CHCH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MIA40_YEAST | |
Accessioni | P36046Primary (citable) accession number: P36046 Secondary accession number(s): D6VX05 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | March 21, 2006 | |
Last modified: | May 25, 2022 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references