UniProtKB - P36037 (DOA1_YEAST)
Protein DOA1
DOA1
Functioni
Ubiquitin-binding protein involved in protein ubiquitination, sorting and degradation (PubMed:2111732, PubMed:8890162, PubMed:19805280, PubMed:18508771, PubMed:20508643, PubMed:27044889).
Acts as a ubiquitinated substrate-recruiting adapter for chaperone ATPase CDC48 by binding mono- or polyubiquitin chains (PubMed:15096053, PubMed:16427015, PubMed:16428438, PubMed:27044889).
Depending on the context, promotes or prevents proteasomal degradation of ubiquitinated proteins (PubMed:2111732, PubMed:8890162, PubMed:19805280, PubMed:27044889).
Involved in the ubiquitin fusion degradation (UFD) pathway by promoting the degradation of ubiquitinated proteins (PubMed:2111732, PubMed:8890162, PubMed:19805280, PubMed:27044889).
Involved in the mitochondria-associated degradation pathway (MAD) by promoting the degradation of several ubiquitinated membrane proteins (PubMed:27044889).
By competing with UFD2 to bind CDC48, prevents the multi-ubiquitination and subsequent degradation of UFD2-dependent substrates (PubMed:16427015).
Required for ribophagy, a process which relocalizes ribosomal particles into the vacuole for degradation in response to starvation (PubMed:20508643).
Involved in the ubiquitin-mediated sorting of membrane proteins into multivesicular bodies (MVBs) (PubMed:18508771).
In addition, plays an essential role in maintaining cellular ubiquitin levels (PubMed:7615550, PubMed:16427015, PubMed:16428438, PubMed:18508771, PubMed:19805280).
May affect indirectly the degradation of ubiquitinylated proteins by regulating cellular ubiquitin levels (PubMed:7615550, PubMed:23525333).
11 PublicationsMiscellaneous
GO - Molecular functioni
- protein-containing complex binding Source: UniProtKB
- ubiquitin binding Source: SGD
- ubiquitin-dependent protein binding Source: UniProtKB
GO - Biological processi
- double-strand break repair via nonhomologous end joining Source: SGD
- mitochondria-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
- ribophagy Source: SGD
- ubiquitin recycling Source: SGD
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-SCE-8951664, Neddylation R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
TCDBi | 3.A.16.1.6, the endoplasmic reticular retrotranslocon (er-rt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Protein DOA11 PublicationAlternative name(s): Degradation of alpha protein 11 Publication Ubiquitin fusion degradation protein 31 Publication |
Gene namesi | Ordered Locus Names:YKL213CImported |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001696, DOA1 |
VEuPathDBi | FungiDB:YKL213C |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Endosome
- Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Nucleus
- Nucleus 3 Publications
Cytoplasm and Cytosol
- Cytoplasm 3 Publications
Note: Predominantly localizes to the cytoplasm. Probably localizes to endosomes and mitochondria in a transient manner.2 Publications
Endosome
- endosome membrane Source: UniProtKB-SubCell
Mitochondrion
- cytoplasmic side of mitochondrial outer membrane Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endosome, Membrane, Mitochondrion, Mitochondrion outer membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 – 425 | Missing : No binding to ubiquitin but does not affect the interaction with CDC48. 1 PublicationAdd BLAST | 424 | |
Mutagenesisi | 2 – 359 | Missing : No binding to ubiquitin. 1 PublicationAdd BLAST | 358 | |
Mutagenesisi | 2 – 353 | Missing : No binding to ubiquitin. 1 PublicationAdd BLAST | 352 | |
Mutagenesisi | 2 – 330 | Missing : Does not affect binding to ubiquitin. 1 PublicationAdd BLAST | 329 | |
Mutagenesisi | 2 – 287 | Missing : No binding to ubiquitin. 1 PublicationAdd BLAST | 286 | |
Mutagenesisi | 5 | L → S: No binding to ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 15 | D → S: No binding to ubiquitin. Reduces cell growth at high temperatures (37 degrees Celsius). Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with A-222 and A-265. 2 Publications | 1 | |
Mutagenesisi | 158 | A → E: No binding to ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 222 | F → A: No binding to ubiquitin. Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with S-15 and A-265. 2 Publications | 1 | |
Mutagenesisi | 237 | C → Y in ufd3-2; severe reduction in the degradation of short-lived ubiquitin-fusion proteins. No defect in the interaction with CDC48. 1 Publication | 1 | |
Mutagenesisi | 265 | W → A: No binding to ubiquitin. Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with S-15 and A-222. 2 Publications | 1 | |
Mutagenesisi | 281 | D → S: No binding to ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 289 – 715 | Missing : No binding to ubiquitin. 1 PublicationAdd BLAST | 427 | |
Mutagenesisi | 417 | F → D: Prevents binding to mono-ubiquitin, reduces levels of free ubiquitin, prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 and is partially sensitive to misfolded protein or translation inhibition-induced stresses; when associated with D-434. 2 Publications | 1 | |
Mutagenesisi | 426 – 715 | Missing : No binding to ubiquitin and no interaction with CDC48. 2 PublicationsAdd BLAST | 290 | |
Mutagenesisi | 434 – 443 | Missing : Loss of interaction with HSE1 and ubiquitin without affecting general ubquitination levels. Prevents protease CSP1 sorting into the vacuole. 1 Publication | 10 | |
Mutagenesisi | 434 – 440 | FILKNTN → AAAKAAA: Loss of interaction with HSE1 without affecting binding to ubiquitin or general ubquitination levels. Prevents protease CSP1 sorting into the vacuole. 1 Publication | 7 | |
Mutagenesisi | 434 | F → D: Prevents binding to mono-ubiquitin, reduces levels of free ubiquitin, prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 and is partially sensitive to misfolded protein or translation inhibition-induced stresses; when associated with D-417. 2 Publications | 1 | |
Mutagenesisi | 451 – 715 | Missing : Does not affect binding to ubiquitin. 1 PublicationAdd BLAST | 265 | |
Mutagenesisi | 495 – 715 | Missing : Loss of interaction with CDC48 without affecting binding to ubiquitin. 1 PublicationAdd BLAST | 221 | |
Mutagenesisi | 541 | R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Prevents the interaction with CDC48 and UFD1 and thus the degradation of mitochondrial proteins FZO1, MDM34 and MSP1; when associated with A-669. 2 Publications | 1 | |
Mutagenesisi | 669 | R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Prevents the interaction with CDC48 and UFD1 and thus the degradation of mitochondrial proteins FZO1, MDM34 and MSP1; when associated with A-541. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050958 | 1 – 715 | Protein DOA1Add BLAST | 715 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 332 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P36037 |
PaxDbi | P36037 |
PRIDEi | P36037 |
PTM databases
iPTMneti | P36037 |
Interactioni
Subunit structurei
Forms a complex composed of CDC48, NPL4, UFD1, DOA1, SHP1 and deubiquitinase OTU1; within the complex interacts with CDC48 (PubMed:16427015).
Interacts (via PUL domain) with CDC48 (via C-terminus); the interaction is direct (PubMed:8890162, PubMed:16427015, PubMed:16428438, PubMed:27044889, PubMed:19805280, PubMed:20508643).
Forms a complex composed of CDC48, DOA1, deubiquitinase UBP3 and probably BRE5; within the complex interacts with CDC48 and UBP3 (PubMed:20508643). May form a complex composed of VPS27, HSE1 and DOA1 (PubMed:18508771).
Interacts with HSE1 (via SH3 domain) (PubMed:18508771).
Interacts (via WD repeats and PFU domain) with ubiquitin; the interaction is direct (PubMed:15096053, PubMed:16427015, PubMed:16428438, PubMed:21070969).
Interacts with ubiquitinated FZO1 but not unmodified FZO1; the interaction recruits FZO1 to CDC48 and promotes FZO1 proteasomal degradation (PubMed:27044889).
9 PublicationsBinary interactionsi
P36037
With | #Exp. | IntAct |
---|---|---|
CDC48 [P25694] | 6 | EBI-6017,EBI-4308 |
HSE1 [P38753] | 3 | EBI-6017,EBI-1382 |
UBP3 [Q01477] | 4 | EBI-6017,EBI-19834 |
Vcp [Q01853] from Mus musculus. | 2 | EBI-6017,EBI-80597 |
GO - Molecular functioni
- ubiquitin binding Source: SGD
- ubiquitin-dependent protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 33952, 701 interactors |
DIPi | DIP-6274N |
IntActi | P36037, 10 interactors |
MINTi | P36037 |
STRINGi | 4932.YKL213C |
Miscellaneous databases
RNActi | P36037, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P36037 |
SMRi | P36037 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P36037 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 11 – 40 | WD 1Sequence analysisAdd BLAST | 30 | |
Repeati | 53 – 82 | WD 2Sequence analysisAdd BLAST | 30 | |
Repeati | 97 – 125 | WD 3Sequence analysisAdd BLAST | 29 | |
Repeati | 135 – 166 | WD 4Sequence analysisAdd BLAST | 32 | |
Repeati | 177 – 206 | WD 5Sequence analysisAdd BLAST | 30 | |
Repeati | 218 – 247 | WD 6Sequence analysisAdd BLAST | 30 | |
Repeati | 259 – 288 | WD 7Sequence analysisAdd BLAST | 30 | |
Domaini | 352 – 449 | PFUPROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 465 – 715 | PULPROSITE-ProRule annotationAdd BLAST | 251 | |
Repeati | 478 – 512 | ARM 11 Publication1 PublicationAdd BLAST | 35 | |
Repeati | 513 – 543 | ARM 21 Publication1 PublicationAdd BLAST | 31 | |
Repeati | 544 – 582 | ARM 31 Publication1 PublicationAdd BLAST | 39 | |
Repeati | 583 – 635 | ARM 41 Publication1 PublicationAdd BLAST | 53 | |
Repeati | 636 – 680 | ARM 51 Publication1 PublicationAdd BLAST | 45 | |
Repeati | 681 – 715 | ARM 61 Publication1 PublicationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 434 – 440 | Interaction with HSE11 Publication | 7 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0301, Eukaryota |
GeneTreei | ENSGT00550000074944 |
HOGENOMi | CLU_011791_2_0_1 |
InParanoidi | P36037 |
OMAi | WSKVGDV |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 2.130.10.10, 1 hit 3.10.20.870, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR020472, G-protein_beta_WD-40_rep IPR015155, PFU IPR038122, PFU_sf IPR013535, PUL_dom IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF09070, PFU, 1 hit PF08324, PUL, 1 hit PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS51394, PFU, 1 hit PS51396, PUL, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV
60 70 80 90 100
YTGQGFLNSV CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG
110 120 130 140 150
NVCSLSFQDG VVISGSWDKT AKVWKEGSLV YNLQAHNASV WDAKVVSFSE
160 170 180 190 200
NKFLTASADK TIKLWQNDKV IKTFSGIHND VVRHLAVVDD GHFISCSNDG
210 220 230 240 250
LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED RTVRIWSKEN
260 270 280 290 300
GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI
310 320 330 340 350
NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI
360 370 380 390 400
EAHQFSNSSW KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL
410 420 430 440 450
KLPINVSDNP YTAADNFLAR YELPMSYRDQ VVQFILKNTN GISLDQPNDN
460 470 480 490 500
ASSSAVSPSK TSVMKVLPVK QYLIMENYNP DTIFNGIVKI NSNEKTFDDE
510 520 530 540 550
ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV RLIVKKLPYS
560 570 580 590 600
SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF
610 620 630 640 650
ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI
660 670 680 690 700
NTKYGPLEEY QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS
710
YGNVPRFKDI FDDLS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 180 | D → DI AA sequence (PubMed:15096053).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39947 Genomic DNA Translation: AAA82258.1 X75951 Genomic DNA Translation: CAA53560.1 Z28213 Genomic DNA Translation: CAA82058.1 BK006944 Genomic DNA Translation: DAA08956.1 |
PIRi | S38051 |
RefSeqi | NP_012709.1, NM_001179778.1 |
Genome annotation databases
EnsemblFungii | YKL213C_mRNA; YKL213C; YKL213C |
GeneIDi | 853667 |
KEGGi | sce:YKL213C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39947 Genomic DNA Translation: AAA82258.1 X75951 Genomic DNA Translation: CAA53560.1 Z28213 Genomic DNA Translation: CAA82058.1 BK006944 Genomic DNA Translation: DAA08956.1 |
PIRi | S38051 |
RefSeqi | NP_012709.1, NM_001179778.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GAE | X-ray | 1.60 | A/B | 464-715 | [»] | |
3L3F | X-ray | 1.90 | X | 354-715 | [»] | |
3ODT | X-ray | 1.35 | A/B | 2-300 | [»] | |
3PSP | X-ray | 2.42 | A | 325-715 | [»] | |
3PST | X-ray | 2.00 | A | 325-715 | [»] | |
AlphaFoldDBi | P36037 | |||||
SMRi | P36037 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33952, 701 interactors |
DIPi | DIP-6274N |
IntActi | P36037, 10 interactors |
MINTi | P36037 |
STRINGi | 4932.YKL213C |
Protein family/group databases
TCDBi | 3.A.16.1.6, the endoplasmic reticular retrotranslocon (er-rt) family |
PTM databases
iPTMneti | P36037 |
Proteomic databases
MaxQBi | P36037 |
PaxDbi | P36037 |
PRIDEi | P36037 |
Genome annotation databases
EnsemblFungii | YKL213C_mRNA; YKL213C; YKL213C |
GeneIDi | 853667 |
KEGGi | sce:YKL213C |
Organism-specific databases
SGDi | S000001696, DOA1 |
VEuPathDBi | FungiDB:YKL213C |
Phylogenomic databases
eggNOGi | KOG0301, Eukaryota |
GeneTreei | ENSGT00550000074944 |
HOGENOMi | CLU_011791_2_0_1 |
InParanoidi | P36037 |
OMAi | WSKVGDV |
Enzyme and pathway databases
Reactomei | R-SCE-8951664, Neddylation R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
EvolutionaryTracei | P36037 |
PROi | PR:P36037 |
RNActi | P36037, protein |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 2.130.10.10, 1 hit 3.10.20.870, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR020472, G-protein_beta_WD-40_rep IPR015155, PFU IPR038122, PFU_sf IPR013535, PUL_dom IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF09070, PFU, 1 hit PF08324, PUL, 1 hit PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS51394, PFU, 1 hit PS51396, PUL, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DOA1_YEAST | |
Accessioni | P36037Primary (citable) accession number: P36037 Secondary accession number(s): D6VWZ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families