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UniProtKB - P36022 (DYHC_YEAST)

Protein

Dynein heavy chain, cytoplasmic

Gene

DYN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.1 Publication

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1796 – 1803ATPSequence analysis8
Nucleotide bindingi2074 – 2081ATPSequence analysis8
Nucleotide bindingi2418 – 2425ATPSequence analysis8
Nucleotide bindingi2760 – 2767ATPSequence analysis8

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionMotor protein
Biological processKaryogamy
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32023-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein heavy chain, cytoplasmic
Alternative name(s):
Dynein heavy chain, cytosolic
Short name:
DYHC
Gene namesi
Name:DYN1
Synonyms:DHC1
Ordered Locus Names:YKR054C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR054C
SGDiS000001762 DYN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001146431 – 4092Dynein heavy chain, cytoplasmicAdd BLAST4092

Proteomic databases

MaxQBiP36022
PaxDbiP36022
PRIDEiP36022

PTM databases

iPTMnetiP36022

Interactioni

Subunit structurei

The dynein complex consists of at least two heavy chains and a number of intermediate and light chains. Interacts with DYN3.1 Publication

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi34185, 347 interactors
ComplexPortaliCPX-1178 Cytoplasmic dynein complex
DIPiDIP-2644N
IntActiP36022, 11 interactors
MINTiP36022
STRINGi4932.YKR054C

Structurei

Secondary structure

14092
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP36022
SMRiP36022
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 1757StemBy similarityAdd BLAST1757
Regioni1758 – 1979AAA 1By similarityAdd BLAST222
Regioni2036 – 2273AAA 2By similarityAdd BLAST238
Regioni2379 – 2628AAA 3By similarityAdd BLAST250
Regioni2722 – 2984AAA 4By similarityAdd BLAST263
Regioni2993 – 3300StalkBy similarityAdd BLAST308
Regioni3370 – 3599AAA 5By similarityAdd BLAST230
Regioni3760 – 3970AAA 6By similarityAdd BLAST211

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili154 – 175Sequence analysisAdd BLAST22
Coiled coili486 – 508Sequence analysisAdd BLAST23
Coiled coili542 – 566Sequence analysisAdd BLAST25
Coiled coili932 – 959Sequence analysisAdd BLAST28
Coiled coili1042 – 1063Sequence analysisAdd BLAST22
Coiled coili1681 – 1705Sequence analysisAdd BLAST25
Coiled coili2993 – 3092Sequence analysisAdd BLAST100
Coiled coili3242 – 3300Sequence analysisAdd BLAST59
Coiled coili3532 – 3608Sequence analysisAdd BLAST77

Domaini

Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.

Sequence similaritiesi

Belongs to the dynein heavy chain family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00910000144015
HOGENOMiHOG000176055
InParanoidiP36022
KOiK10413
OMAiQKRQWII
OrthoDBiEOG092C00NZ

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR035699 AAA_6
IPR011704 ATPase_dyneun-rel_AAA
IPR035706 DHC_D5
IPR026983 DHC_fam
IPR024743 Dynein_HC_stalk
IPR024317 Dynein_heavy_chain_D4_dom
IPR004273 Dynein_heavy_dom
IPR013594 Dynein_heavy_dom-1
IPR013602 Dynein_heavy_dom-2
IPR027417 P-loop_NTPase
PANTHERiPTHR10676 PTHR10676, 1 hit
PfamiView protein in Pfam
PF07728 AAA_5, 1 hit
PF12774 AAA_6, 1 hit
PF12780 AAA_8, 1 hit
PF12781 AAA_9, 1 hit
PF08385 DHC_N1, 1 hit
PF08393 DHC_N2, 1 hit
PF03028 Dynein_heavy, 1 hit
PF12777 MT, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 3 hits
SUPFAMiSSF52540 SSF52540, 5 hits

Sequencei

Sequence statusi: Complete.

P36022-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCKNEARLAN ELIEFVAATV TGIKNSPKEN EQAFIDYLHC QYLERFQFFL 
        60         70         80         90        100
GLLDGREFDT LFVFLFEELD RTIVTIDIGE EAIYDANLAN KKYSTLLIIK 
       110        120        130        140        150
SRSVIVDAEP IATQISAIYL PGPVNAGNLA SIITHGVSSV FGQLIKSDTK 
       160        170        180        190        200
TYSVETIDKT RRKLDDISKQ FQQLHTSIET PDLLAMVPSI IKLAVSKGAT 
       210        220        230        240        250
SHDYANYLPS NDLESMRFLN ILQSIANKWF LVLKQTLAID RDIKNGSFLD 
       260        270        280        290        300
EVEFWSNFYE VLKSLIEQTQ SQEFQVCLSV LTNAKRFHNL TNLLNEGSLS 
       310        320        330        340        350
DKFKLADKYN QFLSSIPIDE VRQASNLEDL QELFPVLASS LKKFRYSGYP 
       360        370        380        390        400
VQRFVVLMDK ISQEVMDAIL SNLSDLFQLE YGSFLGLYEK SAGMIEEWDD 
       410        420        430        440        450
IVQDVNLLIR EDLRKRAPQE LLIQKLTFTS ASVKATLDEI LSTRKRFFSL 
       460        470        480        490        500
AETIKSISPS TYHEEIQRLY HPFEQIHDIS VNFRLKLEQA ESEFSKNMLD 
       510        520        530        540        550
LEKKLQNTLA SFMDSDHCPT EKLSYLVKFK PLMELCPRIK VKVLENQQIL 
       560        570        580        590        600
LLEIKKDIRQ LETGLELLPK ILHVEALNNI PPISARISYF LNVQSRIDNI 
       610        620        630        640        650
VQYLEALFGS NWNDTLEGRS ISTSIVQLRK ETNPHDVFLH WLGNFPEKAT 
       660        670        680        690        700
ANLLTTPILK LIRNNEDDYE LKVNFDFALA AAYSELRSLT YMAFQVPSHI 
       710        720        730        740        750
VRIARTYMYL YPRAINLVEL IQTFFSLSKS LSYTFYTNIF LKRNVQTVWL 
       760        770        780        790        800
LLQQILITPW ESLQEESSEM SCSVHSLARL EKSIDGILSD YQILKNSEPQ 
       810        820        830        840        850
FAKEFSGLKS FDGTADDLHE VEEIISNIQA IFENLFTKGL TNVSDHISTF 
       860        870        880        890        900
NNLIISIILE KVRLNLKKMH FPKHVLKLSF NEGRITSSPS LAAMKRSLLK 
       910        920        930        940        950
DIEALLNKVV LINFLHDPDH PLSTTLTFNS LVIKLKDDIQ NCIEQVQNLH 
       960        970        980        990       1000
CKINSYVKEW QKMEFLWQIT EEAFLEVVDN STQRCFGILK GLLDSQSKFD 
      1010       1020       1030       1040       1050
LIISRNNFSK NLVLHTEDAQ RHIRSKMDSW ILYVSKHLLT IYERDARKLH 
      1060       1070       1080       1090       1100
EDMNRDREAV EDMDINFTSL KNITVIIEAV NVNKRHLTER DIQIKLLGSV 
      1110       1120       1130       1140       1150
MRALTKLKVR FPSHFVYIDQ LDNDFSSLRQ SLSYVEQELQ KHRVVIAKSL 
      1160       1170       1180       1190       1200
EEGVENINNL SQSLNESWSV RKPISPTLTP PEALKILEFF NESITKLKKK 
      1210       1220       1230       1240       1250
MHSVAAAAKM LLIPVVLNDQ LTHVVEEVKT YDLVWRSIKN LWEDVQRTFE 
      1260       1270       1280       1290       1300
TPWCRVDVLL LQSDLANFLR RADELPRAVK QFEMYKSLFS QVNMLTSVNK 
      1310       1320       1330       1340       1350
ILVELKDGAL KPRHWNMIFR DIGKRQIQKN LLDKLEFSLK DVMVLNLTLN 
      1360       1370       1380       1390       1400
EILLTKIIER AQKEFVIEKS LNRIKKFWKE AQYEVIEHSS GLKLVREWDV 
      1410       1420       1430       1440       1450
LEQACKEDLE ELVSMKASNY YKIFEQDCLD LESKLTKLSE IQVNWVEVQF 
      1460       1470       1480       1490       1500
YWLDLYGILG ENLDIQNFLP LETSKFKSLT SEYKMITTRA FQLDTTIEVI 
      1510       1520       1530       1540       1550
HIPNFDTTLK LTIDSLKMIK SSLSTFLERQ RRQFPRFYFL GNDDLLKIIG 
      1560       1570       1580       1590       1600
SGKHHDQVSK FMKKMFGSIE SIIFLEDFIT GVRSVEGEVL NLNEKIELKD 
      1610       1620       1630       1640       1650
SIQAQEWLNI LDTEIKLSVF TQFRDCLGQL KDGTDIEVVV SKYIFQAILL 
      1660       1670       1680       1690       1700
SAQVMWTELV EKCLQTNQFS KYWKEVDMKI KGLLDKLNKS SDNVKKKIEA 
      1710       1720       1730       1740       1750
LLVEYLHFNN VIGQLKNCST KEEARLLWAK VQKFYQKNDT LDDLNSVFIS 
      1760       1770       1780       1790       1800
QSGYLLQYKF EYIGIPERLI YTPLLLIGFA TLTDSLHQKY GGCFFGPAGT 
      1810       1820       1830       1840       1850
GKTETVKAFG QNLGRVVVVF NCDDSFDYQV LSRLLVGITQ IGAWGCFDEF 
      1860       1870       1880       1890       1900
NRLDEKVLSA VSANIQQIQN GLQVGKSHIT LLEEETPLSP HTAVFITLNP 
      1910       1920       1930       1940       1950
GYNGRSELPE NLKKSFREFS MKSPQSGTIA EMILQIMGFE DSKSLASKIV 
      1960       1970       1980       1990       2000
HFLELLSSKC SSMNHYHFGL RTLKGVLRNC SPLISEFGEG EKTVVESLKR 
      2010       2020       2030       2040       2050
VILPSLGDTD ELVFKDELSK IFDSAGTPLN SKAIVQCLKD AGQRSGFSMS 
      2060       2070       2080       2090       2100
EEFLKKCMQF YYMQKTQQAL ILVGKAGCGK TATWKTVIDA MAIFDGHANV 
      2110       2120       2130       2140       2150
VYVIDTKVLT KESLYGSMLK ATLEWRDGLF TSILRRVNDD ITGTFKNSRI 
      2160       2170       2180       2190       2200
WVVFDSDLDP EYVEAMNSVL DDNKILTLPN GERLPIPPNF RILFETDNLD 
      2210       2220       2230       2240       2250
HTTPATITRC GLLWFSTDVC SISSKIDHLL NKSYEALDNK LSMFELDKLK 
      2260       2270       2280       2290       2300
DLISDSFDMA SLTNIFTCSN DLVHILGVRT FNKLETAVQL AVHLISSYRQ 
      2310       2320       2330       2340       2350
WFQNLDDKSL KDVITLLIKR SLLYALAGDS TGESQRAFIQ TINTYFGHDS 
      2360       2370       2380       2390       2400
QELSDYSTIV IANDKLSFSS FCSEIPSVSL EAHEVMRPDI VIPTIDTIKH 
      2410       2420       2430       2440       2450
EKIFYDLLNS KRGIILCGPP GSGKTMIMNN ALRNSSLYDV VGINFSKDTT 
      2460       2470       2480       2490       2500
TEHILSALHR HTNYVTTSKG LTLLPKSDIK NLVLFCDEIN LPKLDKYGSQ 
      2510       2520       2530       2540       2550
NVVLFLRQLM EKQGFWKTPE NKWVTIERIH IVGACNPPTD PGRIPMSERF 
      2560       2570       2580       2590       2600
TRHAAILYLG YPSGKSLSQI YEIYYKAIFK LVPEFRSYTE PFARASVHLY 
      2610       2620       2630       2640       2650
NECKARYSTG LQSHYLFSPR ELTRLVRGVY TAINTGPRQT LRSLIRLWAY 
      2660       2670       2680       2690       2700
EAWRIFADRL VGVKEKNSFE QLLYETVDKY LPNQDLGNIS STSLLFSGLL 
      2710       2720       2730       2740       2750
SLDFKEVNKT DLVNFIEERF KTFCDEELEV PMVIHESMVD HILRIDRALK 
      2760       2770       2780       2790       2800
QVQGHMMLIG ASRTGKTILT RFVAWLNGLK IVQPKIHRHS NLSDFDMILK 
      2810       2820       2830       2840       2850
KAISDCSLKE SRTCLIIDES NILETAFLER MNTLLANADI PDLFQGEEYD 
      2860       2870       2880       2890       2900
KLLNNLRNKT RSLGLLLDTE QELYDWFVGE IAKNLHVVFT ICDPTNNKSS 
      2910       2920       2930       2940       2950
AMISSPALFN RCIINWMGDW DTKTMSQVAN NMVDVIPMEF TDFIVPEVNK 
      2960       2970       2980       2990       3000
ELVFTEPIQT IRDAVVNILI HFDRNFYQKM KVGVNPRSPG YFIDGLRALV 
      3010       3020       3030       3040       3050
KLVTAKYQDL QENQRFVNVG LEKLNESVLK VNELNKTLSK KSTELTEKEK 
      3060       3070       3080       3090       3100
EARSTLDKML MEQNESERKQ EATEEIKKIL KVQEEDIRKR KEVVMKSIQD 
      3110       3120       3130       3140       3150
IEPTILEAQR GVKNIKKQQL TEIRSMVNPP SGVKIVMEAV CAILGYQFSN 
      3160       3170       3180       3190       3200
WRDIQQFIRK DDFIHNIVHY DTTLHMKPQI RKYMEEEFLS DPNFTYETIN 
      3210       3220       3230       3240       3250
RASKACGPLY QWVNAQINFS KVLENVDPLR QEMKRIEFES LKTKANLLAA 
      3260       3270       3280       3290       3300
EEMTQDLEAS IEVSKRKYSL LIRDVEAIKT EMSNVQANLD RSISLVKSLT 
      3310       3320       3330       3340       3350
FEKERWLNTT KQFSKTSQEL IGNCIISSIY ETYFGHLNER ERADMLVILK 
      3360       3370       3380       3390       3400
RLLGKFAVKY DVNYRFIDYL VTLDEKMKWL ECGLDKNDYF LENMSIVMNS 
      3410       3420       3430       3440       3450
QDAVPFLLDP SSHMITVISN YYGNKTVLLS FLEEGFVKRL ENAIRFGSVV 
      3460       3470       3480       3490       3500
IIQDGEFFDP IISRLISREF NHAGNRVTVE IGDHEVDVSG DFKLFIHSCD 
      3510       3520       3530       3540       3550
PSGDIPIFLR SRVRLVHFVT NKESIETRIF DITLTEENAE MQRKREDLIK 
      3560       3570       3580       3590       3600
LNTEYKLKLK NLEKRLLEEL NNSQGNMLEN DELMVTLNNL KKEAMNIEKK 
      3610       3620       3630       3640       3650
LSESEEFFPQ FDNLVEEYSI IGKHSVKIFS MLEKFGQFHW FYGISIGQFL 
      3660       3670       3680       3690       3700
SCFKRVFIKK SRETRAARTR VDEILWLLYQ EVYCQFSTAL DKKFKMIMAM 
      3710       3720       3730       3740       3750
TMFCLYKFDI ESEQYKEAVL TMIGVLSESS DGVPKLTVDT NNDLRYLWDY 
      3760       3770       3780       3790       3800
VTTKSYISAL NWFKNEFFVD EWNIADVVAN SENNYFTMAS ERDVDGTFKL 
      3810       3820       3830       3840       3850
IELAKASKES LKIIPLGSIE NLNYAQEEIS KSKIEGGWIL LQNIQMSLSW 
      3860       3870       3880       3890       3900
VKTYLHKHVE ETKAAEEHEK FKMFMTCHLT GDKLPAPLLQ RTDRFVYEDI 
      3910       3920       3930       3940       3950
PGILDTVKDL WGSQFFTGKI SGVWSVYCTF LLSWFHALIT ARTRLVPHGF 
      3960       3970       3980       3990       4000
SKKYYFNDCD FQFASVYLEN VLATNSTNNI PWAQVRDHIA TIVYGGKIDE 
      4010       4020       4030       4040       4050
EKDLEVVAKL CAHVFCGSDN LQIVPGVRIP QPLLQQSEEE ERARLTAILS 
      4060       4070       4080       4090 
NTIEPADSLS SWLQLPRESI LNYERLQAKE VASSTEQLLQ EM
Length:4,092
Mass (Da):471,348
Last modified:June 1, 1994 - v1
Checksum:i3D9DF447E8E2D6BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti589Y → C in AAA16055 (PubMed:8234262).Curated1
Sequence conflicti601V → A in AAA16055 (PubMed:8234262).Curated1
Sequence conflicti1364E → A in AAA16055 (PubMed:8234262).Curated1
Sequence conflicti2118 – 2119ML → IV in CAA79923 (PubMed:8248224).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21877 Genomic DNA Translation: CAA79923.1
L15626 Unassigned DNA Translation: AAA16055.1
Z28279 Genomic DNA Translation: CAA82132.1
BK006944 Genomic DNA Translation: DAA09205.1
PIRiS38128
RefSeqiNP_012980.1, NM_001179844.1

Genome annotation databases

EnsemblFungiiYKR054C; YKR054C; YKR054C
GeneIDi853928
KEGGisce:YKR054C

Similar proteinsi

Entry informationi

Entry nameiDYHC_YEAST
AccessioniPrimary (citable) accession number: P36022
Secondary accession number(s): D6VXB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 12, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health

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