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Protein

Heat shock protein 70 homolog LHS1

Gene

LHS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chaperone required for protein translocation and folding in the endoplasmic reticulum.1 Publication

Miscellaneous

Present with 137 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

  • adenyl-nucleotide exchange factor activity Source: SGD
  • ATPase activity Source: UniProtKB-EC
  • ATP binding Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

Keywordsi

Molecular functionChaperone, Hydrolase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31869-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 70 homolog LHS1 (EC:3.6.1.3)
Gene namesi
Name:LHS1
Ordered Locus Names:YKL073W
ORF Names:YKL355
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL073W
SGDiS000001556 LHS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi239G → D in allele LHS1-1; constitutive ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001355621 – 881Heat shock protein 70 homolog LHS1Add BLAST861

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi128N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi458N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi474N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi481N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi489N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi527N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi844N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP36016
PaxDbiP36016
PRIDEiP36016

Expressioni

Inductioni

By the unfolded protein response (UPR).1 Publication

Interactioni

Subunit structurei

Interacts with the heat shock protein 70 (HSP70) KAR2, and this stimulates nucleotide exchange on KAR2. KAR2 in turn acts to stimulate the ATPase activity of LHS1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34058, 373 interactors
DIPiDIP-6520N
IntActiP36016, 7 interactors
MINTiP36016
STRINGi4932.YKL073W

Structurei

3D structure databases

ProteinModelPortaliP36016
SMRiP36016
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi878 – 881Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00920000150184
HOGENOMiHOG000111144
InParanoidiP36016
KOiK09486
OMAiNADESCA
OrthoDBiEOG092C0K07

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36016-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRNVLRLLFL TAFVAIGSLA AVLGVDYGQQ NIKAIVVSPQ APLELVLTPE
60 70 80 90 100
AKRKEISGLS IKRLPGYGKD DPNGIERIYG SAVGSLATRF PQNTLLHLKP
110 120 130 140 150
LLGKSLEDET TVTLYSKQHP GLEMVSTNRS TIAFLVDNVE YPLEELVAMN
160 170 180 190 200
VQEIANRANS LLKDRDARTE DFVNKMSFTI PDFFDQHQRK ALLDASSITT
210 220 230 240 250
GIEETYLVSE GMSVAVNFVL KQRQFPPGEQ QHYIVYDMGS GSIKASMFSI
260 270 280 290 300
LQPEDTTQPV TIEFEGYGYN PHLGGAKFTM DIGSLIENKF LETHPAIRTD
310 320 330 340 350
ELHANPKALA KINQAAEKAK LILSANSEAS INIESLINDI DFRTSITRQE
360 370 380 390 400
FEEFIADSLL DIVKPINDAV TKQFGGYGTN LPEINGVILA GGSSRIPIVQ
410 420 430 440 450
DQLIKLVSEE KVLRNVNADE SAVNGVVMRG IKLSNSFKTK PLNVVDRSVN
460 470 480 490 500
TYSFKLSNES ELYDVFTRGS AYPNKTSILT NTTDSIPNNF TIDLFENGKL
510 520 530 540 550
FETITVNSGA IKNSYSSDKC SSGVAYNITF DLSSDRLFSI QEVNCICQSE
560 570 580 590 600
NDIGNSKQIK NKGSRLAFTS EDVEIKRLSP SERSRLHEHI KLLDKQDKER
610 620 630 640 650
FQFQENLNVL ESNLYDARNL LMDDEVMQNG PKSQVEELSE MVKVYLDWLE
660 670 680 690 700
DASFDTDPED IVSRIREIGI LKKKIELYMD SAKEPLNSQQ FKGMLEEGHK
710 720 730 740 750
LLQAIETHKN TVEEFLSQFE TEFADTIDNV REEFKKIKQP AYVSKALSTW
760 770 780 790 800
EETLTSFKNS ISEIEKFLAK NLFGEDLREH LFEIKLQFDM YRTKLEEKLR
810 820 830 840 850
LIKSGDESRL NEIKKLHLRN FRLQKRKEEK LKRKLEQEKS RNNNETESTV
860 870 880
INSADDKTTI VNDKTTESNP SSEEDILHDE L
Length:881
Mass (Da):99,572
Last modified:June 1, 1994 - v1
Checksum:iACED092CA3A34785
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75780 Genomic DNA Translation: CAA53401.1
Z28073 Genomic DNA Translation: CAA81910.1
BK006944 Genomic DNA Translation: DAA09083.1
PIRiS37895
RefSeqiNP_012850.1, NM_001179639.1

Genome annotation databases

EnsemblFungiiYKL073W; YKL073W; YKL073W
GeneIDi853789
KEGGisce:YKL073W

Similar proteinsi

Entry informationi

Entry nameiLHS1_YEAST
AccessioniPrimary (citable) accession number: P36016
Secondary accession number(s): D6VXL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 12, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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