UniProtKB - P36013 (MAOM_YEAST)
Protein
NAD-dependent malic enzyme, mitochondrial
Gene
MAE1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 10500 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Cofactori
Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 187 | Proton donorBy similarity | 1 | |
Active sitei | 259 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 330 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 331 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 354 | Divalent metal cationBy similarity | 1 | |
Binding sitei | 354 | NADBy similarity | 1 | |
Sitei | 354 | Important for activityBy similarity | 1 | |
Binding sitei | 499 | NADBy similarity | 1 |
GO - Molecular functioni
- malate dehydrogenase (decarboxylating) (NAD+) activity Source: GO_Central
- malic enzyme activity Source: SGD
- metal ion binding Source: UniProtKB-KW
- NAD binding Source: InterPro
- oxaloacetate decarboxylase activity Source: UniProtKB-EC
GO - Biological processi
- cellular amino acid metabolic process Source: SGD
- malate metabolic process Source: GO_Central
- pyruvate metabolic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | MetaCyc:YKL029C-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MAE1 Ordered Locus Names:YKL029C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YKL029C |
SGDi | S000001512, MAE1 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 2 Publications
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | Mitochondrion | ||
ChainiPRO_0000160206 | ? – 669 | NAD-dependent malic enzyme, mitochondrial |
Proteomic databases
MaxQBi | P36013 |
PaxDbi | P36013 |
PRIDEi | P36013 |
PTM databases
CarbonylDBi | P36013 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34102, 160 interactors |
DIPi | DIP-4444N |
IntActi | P36013, 74 interactors |
MINTi | P36013 |
STRINGi | 4932.YKL029C |
Miscellaneous databases
RNActi | P36013, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the malic enzymes family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1257, Eukaryota |
GeneTreei | ENSGT00950000183134 |
HOGENOMi | CLU_011405_5_2_1 |
InParanoidi | P36013 |
OMAi | WIREQMW |
Family and domain databases
Gene3Di | 3.40.50.10380, 1 hit |
InterProi | View protein in InterPro IPR015884, Malic_enzyme_CS IPR012301, Malic_N_dom IPR037062, Malic_N_dom_sf IPR012302, Malic_NAD-bd IPR001891, Malic_OxRdtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00390, malic, 1 hit PF03949, Malic_M, 1 hit |
PIRSFi | PIRSF000106, ME, 1 hit |
PRINTSi | PR00072, MALOXRDTASE |
SMARTi | View protein in SMART SM01274, malic, 1 hit SM00919, Malic_M, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00331, MALIC_ENZYMES, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P36013-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRTRLSVSV AARSQLTRSL TASRTAPLRR WPIQQSRLYS SNTRSHKATT
60 70 80 90 100
TRENTFQKPY SDEEVTKTPV GSRARKIFEA PHPHATRLTV EGAIECPLES
110 120 130 140 150
FQLLNSPLFN KGSAFTQEER EAFNLEALLP PQVNTLDEQL ERSYKQLCYL
160 170 180 190 200
KTPLAKNDFM TSLRVQNKVL YFALIRRHIK ELVPIIYTPT EGDAIAAYSH
210 220 230 240 250
RFRKPEGVFL DITEPDSIEC RLATYGGDKD VDYIVVSDSE GILGIGDQGI
260 270 280 290 300
GGVRIAISKL ALMTLCGGIH PGRVLPVCLD VGTNNKKLAR DELYMGNKFS
310 320 330 340 350
RIRGKQYDDF LEKFIKAVKK VYPSAVLHFE DFGVKNARRL LEKYRYELPS
360 370 380 390 400
FNDDIQGTGA VVMASLIAAL KHTNRDLKDT RVLIYGAGSA GLGIADQIVN
410 420 430 440 450
HMVTHGVDKE EARKKIFLMD RRGLILQSYE ANSTPAQHVY AKSDAEWAGI
460 470 480 490 500
NTRSLHDVVE NVKPTCLVGC STQAGAFTQD VVEEMHKHNP RPIIFPLSNP
510 520 530 540 550
TRLHEAVPAD LMKWTNNNAL VATGSPFPPV DGYRISENNN CYSFPGIGLG
560 570 580 590 600
AVLSRATTIT DKMISAAVDQ LAELSPLREG DSRPGLLPGL DTITNTSARL
610 620 630 640 650
ATAVILQALE EGTARIEQEQ VPGGAPGETV KVPRDFDECL QWVKAQMWEP
660
VYRPMIKVQH DPSVHTNQL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28029 Genomic DNA Translation: CAA81865.1 BK006944 Genomic DNA Translation: DAA09125.1 |
PIRi | S37846 |
RefSeqi | NP_012896.1, NM_001179595.1 |
Genome annotation databases
EnsemblFungii | YKL029C_mRNA; YKL029C; YKL029C |
GeneIDi | 853839 |
KEGGi | sce:YKL029C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28029 Genomic DNA Translation: CAA81865.1 BK006944 Genomic DNA Translation: DAA09125.1 |
PIRi | S37846 |
RefSeqi | NP_012896.1, NM_001179595.1 |
3D structure databases
SMRi | P36013 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34102, 160 interactors |
DIPi | DIP-4444N |
IntActi | P36013, 74 interactors |
MINTi | P36013 |
STRINGi | 4932.YKL029C |
PTM databases
CarbonylDBi | P36013 |
Proteomic databases
MaxQBi | P36013 |
PaxDbi | P36013 |
PRIDEi | P36013 |
Genome annotation databases
EnsemblFungii | YKL029C_mRNA; YKL029C; YKL029C |
GeneIDi | 853839 |
KEGGi | sce:YKL029C |
Organism-specific databases
EuPathDBi | FungiDB:YKL029C |
SGDi | S000001512, MAE1 |
Phylogenomic databases
eggNOGi | KOG1257, Eukaryota |
GeneTreei | ENSGT00950000183134 |
HOGENOMi | CLU_011405_5_2_1 |
InParanoidi | P36013 |
OMAi | WIREQMW |
Enzyme and pathway databases
BioCyci | MetaCyc:YKL029C-MONOMER |
Miscellaneous databases
PROi | PR:P36013 |
RNActi | P36013, protein |
Family and domain databases
Gene3Di | 3.40.50.10380, 1 hit |
InterProi | View protein in InterPro IPR015884, Malic_enzyme_CS IPR012301, Malic_N_dom IPR037062, Malic_N_dom_sf IPR012302, Malic_NAD-bd IPR001891, Malic_OxRdtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00390, malic, 1 hit PF03949, Malic_M, 1 hit |
PIRSFi | PIRSF000106, ME, 1 hit |
PRINTSi | PR00072, MALOXRDTASE |
SMARTi | View protein in SMART SM01274, malic, 1 hit SM00919, Malic_M, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00331, MALIC_ENZYMES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAOM_YEAST | |
Accessioni | P36013Primary (citable) accession number: P36013 Secondary accession number(s): D6VXQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD