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UniProtKB - P36003 (NNK1_YEAST)
Protein
Nitrogen network kinase 1
Gene
NNK1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Serine/threonine-protein kinase involved in the phosphorylation of the NAD+-dependent glutamate dehydrogenase GDH2. When overexpressed, confers hypersensitivity to rapamycin and induces rapid nuclear accumulation of GLN3 to activate the transcription of nitrogen-regulated genes.
1 PublicationCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 478 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 580 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 455 – 463 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium-dependent protein serine/threonine kinase activity Source: GO_Central
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein kinase activity Source: GO_Central
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- peptidyl-serine phosphorylation Source: GO_Central
- protein autophosphorylation Source: GO_Central
- protein phosphorylation Source: SGD
- proteolysis Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-171007, p38MAPK events R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-3371453, Regulation of HSF1-mediated heat shock response R-SCE-4420097, VEGFA-VEGFR2 Pathway R-SCE-450302, activated TAK1 mediates p38 MAPK activation R-SCE-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-SCE-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA R-SCE-5687128, MAPK6/MAPK4 signaling |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NNK1 Ordered Locus Names:YKL171W ORF Names:YKL635 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001654, NNK1 |
VEuPathDBi | FungiDB:YKL171W |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086152 | 1 – 928 | Nitrogen network kinase 1Add BLAST | 928 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 178 | PhosphoserineCombined sources | 1 | |
Modified residuei | 179 | PhosphoserineCombined sources | 1 | |
Modified residuei | 405 | PhosphoserineCombined sources | 1 | |
Modified residuei | 426 | PhosphoserineCombined sources | 1 | |
Modified residuei | 737 | PhosphoserineCombined sources | 1 | |
Modified residuei | 739 | PhosphotyrosineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P36003 |
PaxDbi | P36003 |
PRIDEi | P36003 |
PTM databases
iPTMneti | P36003 |
Interactioni
Subunit structurei
Interacts with URE2 and GDH2.
Interacts also with the TORC1 kinase complex.
1 PublicationBinary interactionsi
P36003
With | #Exp. | IntAct |
---|---|---|
GDH2 [P33327] | 3 | EBI-9796,EBI-5815 |
HRR25 [P29295] | 4 | EBI-9796,EBI-8536 |
TOR1 [P35169] | 3 | EBI-9796,EBI-19374 |
URE2 [P23202] | 4 | EBI-9796,EBI-20138 |
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 33967, 221 interactors |
DIPi | DIP-2877N |
IntActi | P36003, 21 interactors |
MINTi | P36003 |
STRINGi | 4932.YKL171W |
Miscellaneous databases
RNActi | P36003, protein |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 449 – 912 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 464 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 43 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 81 – 118 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 374 – 394 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 670 – 741 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 767 – 813 | DisorderedSequence analysisAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 39 | Polar residuesSequence analysisAdd BLAST | 39 | |
Compositional biasi | 86 – 110 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 678 – 717 | Polar residuesSequence analysisAdd BLAST | 40 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000162886 |
HOGENOMi | CLU_010370_0_0_1 |
InParanoidi | P36003 |
OMAi | WGMIREC |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR016241, Nnk1 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000610, Ser/Thr_PK_YKL171w_prd, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P36003-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFTSQRQLRQ NGSPMSSSRS SQHSSGTASP ISDSPASNRS YGRDLRGLMG
60 70 80 90 100
IDIPANEPAF NRANSSDTIY FRPKKIYKME HEHPSRSTLV QLQTRSQPDD
110 120 130 140 150
VASSQVNPEG GTDDLELGDP CGNQSLYTIG AEYVPDLDFT KLVNEWQKST
160 170 180 190 200
EDLYEFRSSA TPQVQIKDSG KGNYELWSSP DAILTQNKLR RDSFSQENSD
210 220 230 240 250
SLSPEDSILS RNLHSKVKPI PLPRNSQQIF TPLSNLEAER RSSYTTSSNN
260 270 280 290 300
NSITQNNKFS FAKLKYSLPT QSSAVPASFD SNASSLNFLP TTTLSTLSEL
310 320 330 340 350
QISPNDMMDL IQKLPRNFLN LPYTQRKKVI IEHAPSHDYK AMMSLVKKFM
360 370 380 390 400
LTSSRSNFSL AGFANNASVS QATANDDNIN SRNTPNNSND TYVNTRPLQR
410 420 430 440 450
SRHGSIASQF LSSFSPSMTS IAKMNSNPLS GSAGGSARPD DKGMEILGHR
460 470 480 490 500
LGKIIGFGAW GIIRECFDIE TGVGRVIKIV KFKGHQNIKK HVLREVAIWR
510 520 530 540 550
TLKHNRILPL LDWKLDDNYA MYCLTERIND GTLYDLVISW DEFKRSKIPF
560 570 580 590 600
AERCRLTIFL SLQLLSALKY MHSKTIVHGD IKLENCLLQK EGKKSDWKVF
610 620 630 640 650
LCDFGMSCHF DEKHVYRNDT FDENLSSGNS HRKRKSIEQT NLIKYPTTNF
660 670 680 690 700
LPDDRTNDFD ASENLKYQFE NRKHQPFTPK GMVSSSSHSL KHLNQPSSSS
710 720 730 740 750
SSNLFHKPAS QPQPQHRSPF HGRHKTTDFS NLEPEPSKYI GSLPYASPEL
760 770 780 790 800
LRYSDARRSK SVEMHIYDSP DSSQSEISAA SSSSSNLSSL SSSTKASAVT
810 820 830 840 850
NSGVTTSSPS GSSTDFPCIV SPLGPASDIW ALGVMLYTML VGKLPFNHEF
860 870 880 890 900
EPRLRSLIKV GEFDRFSLAQ VCKFDRKKNE GTIGQGLYDT VIGCLTIDLD
910 920
KRWKLKRIEE VLQNEMNLSE AIHDNNGS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26878 Genomic DNA Translation: CAA81516.1 Z28171 Genomic DNA Translation: CAA82013.1 BK006944 Genomic DNA Translation: DAA08995.1 |
PIRi | S38001 |
RefSeqi | NP_012750.1, NM_001179737.1 |
Genome annotation databases
EnsemblFungii | YKL171W_mRNA; YKL171W; YKL171W |
GeneIDi | 853683 |
KEGGi | sce:YKL171W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26878 Genomic DNA Translation: CAA81516.1 Z28171 Genomic DNA Translation: CAA82013.1 BK006944 Genomic DNA Translation: DAA08995.1 |
PIRi | S38001 |
RefSeqi | NP_012750.1, NM_001179737.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33967, 221 interactors |
DIPi | DIP-2877N |
IntActi | P36003, 21 interactors |
MINTi | P36003 |
STRINGi | 4932.YKL171W |
PTM databases
iPTMneti | P36003 |
Proteomic databases
MaxQBi | P36003 |
PaxDbi | P36003 |
PRIDEi | P36003 |
Genome annotation databases
EnsemblFungii | YKL171W_mRNA; YKL171W; YKL171W |
GeneIDi | 853683 |
KEGGi | sce:YKL171W |
Organism-specific databases
SGDi | S000001654, NNK1 |
VEuPathDBi | FungiDB:YKL171W |
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000162886 |
HOGENOMi | CLU_010370_0_0_1 |
InParanoidi | P36003 |
OMAi | WGMIREC |
Enzyme and pathway databases
Reactomei | R-SCE-171007, p38MAPK events R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-3371453, Regulation of HSF1-mediated heat shock response R-SCE-4420097, VEGFA-VEGFR2 Pathway R-SCE-450302, activated TAK1 mediates p38 MAPK activation R-SCE-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-SCE-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA R-SCE-5687128, MAPK6/MAPK4 signaling |
Miscellaneous databases
PROi | PR:P36003 |
RNActi | P36003, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR016241, Nnk1 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000610, Ser/Thr_PK_YKL171w_prd, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NNK1_YEAST | |
Accessioni | P36003Primary (citable) accession number: P36003 Secondary accession number(s): D6VX29 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - SIMILARITY comments
Index of protein domains and families