UniProtKB - P35997 (RS27A_YEAST)
40S ribosomal protein S27-A
RPS27A
Functioni
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.
1 PublicationMiscellaneous
Cofactori
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 37 – 59 | C4-typeSequence analysisAdd BLAST | 23 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: GO_Central
- structural constituent of ribosome Source: SGD
GO - Biological processi
- cytoplasmic translation Source: SGD
- maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- ribosomal small subunit assembly Source: SGD
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-1799339, SRP-dependent cotranslational protein targeting to membrane R-SCE-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-SCE-72689, Formation of a pool of free 40S subunits R-SCE-72695, Formation of the ternary complex, and subsequently, the 43S complex R-SCE-72702, Ribosomal scanning and start codon recognition R-SCE-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: 40S ribosomal protein S27-A1 PublicationAlternative name(s): RP61 Small ribosomal subunit protein eS27-A1 Publication YS20 |
Gene namesi | Name:RPS27A1 Publication Ordered Locus Names:YKL156W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001639, RPS27A |
VEuPathDBi | FungiDB:YKL156W |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Cytosol
- cytosol Source: Reactome
- cytosolic small ribosomal subunit Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149066 | 1 – 82 | 40S ribosomal protein S27-AAdd BLAST | 82 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 40 | S-methylcysteine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
MethylationProteomic databases
MaxQBi | P35997 |
PaxDbi | P35997 |
PRIDEi | P35997 |
TopDownProteomicsi | P35997 |
PTM databases
iPTMneti | P35997 |
Interactioni
Subunit structurei
Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).
1 Publication1 PublicationProtein-protein interaction databases
BioGRIDi | 33981, 713 interactors |
IntActi | P35997, 11 interactors |
MINTi | P35997 |
STRINGi | 4932.YKL156W |
Miscellaneous databases
RNActi | P35997, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P35997 |
SMRi | P35997 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 37 – 59 | C4-typeSequence analysisAdd BLAST | 23 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1779, Eukaryota |
GeneTreei | ENSGT00940000167938 |
HOGENOMi | CLU_130128_3_0_1 |
InParanoidi | P35997 |
OMAi | LHPAIDF |
Family and domain databases
Gene3Di | 2.20.25.100, 1 hit |
HAMAPi | MF_00371, Ribosomal_S27e, 1 hit |
InterProi | View protein in InterPro IPR000592, Ribosomal_S27 IPR023407, Ribosomal_S27_Zn-bd_dom_sf IPR011332, Ribosomal_zn-bd |
PANTHERi | PTHR11594, PTHR11594, 1 hit |
Pfami | View protein in Pfam PF01667, Ribosomal_S27e, 1 hit |
SUPFAMi | SSF57829, SSF57829, 1 hit |
PROSITEi | View protein in PROSITE PS01168, RIBOSOMAL_S27E, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA
60 70 80
QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26877 Genomic DNA No translation available. Z28156 Genomic DNA Translation: CAA81998.1 Z28155 Genomic DNA Translation: CAA81997.1 BK006944 Genomic DNA Translation: DAA09008.1 |
PIRi | S37986 |
RefSeqi | NP_012766.1, NM_001179722.1 |
Genome annotation databases
EnsemblFungii | YKL156W_mRNA; YKL156W; YKL156W |
GeneIDi | 853700 |
KEGGi | sce:YKL156W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26877 Genomic DNA No translation available. Z28156 Genomic DNA Translation: CAA81998.1 Z28155 Genomic DNA Translation: CAA81997.1 BK006944 Genomic DNA Translation: DAA09008.1 |
PIRi | S37986 |
RefSeqi | NP_012766.1, NM_001179722.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J6X | electron microscopy | 6.10 | 27 | 1-82 | [»] | |
3J6Y | electron microscopy | 6.10 | 27 | 1-82 | [»] | |
3J77 | electron microscopy | 6.20 | 27 | 1-82 | [»] | |
3J78 | electron microscopy | 6.30 | 27 | 1-82 | [»] | |
4U3M | X-ray | 3.00 | D7/d7 | 2-82 | [»] | |
4U3N | X-ray | 3.20 | D7/d7 | 2-82 | [»] | |
4U3U | X-ray | 2.90 | D7/d7 | 2-82 | [»] | |
4U4N | X-ray | 3.10 | D7/d7 | 2-82 | [»] | |
4U4O | X-ray | 3.60 | D7/d7 | 2-82 | [»] | |
4U4Q | X-ray | 3.00 | D7/d7 | 2-82 | [»] | |
4U4R | X-ray | 2.80 | D7/d7 | 2-82 | [»] | |
4U4U | X-ray | 3.00 | D7/d7 | 2-82 | [»] | |
4U4Y | X-ray | 3.20 | D7/d7 | 2-82 | [»] | |
4U4Z | X-ray | 3.10 | D7/d7 | 2-82 | [»] | |
4U50 | X-ray | 3.20 | D7/d7 | 2-82 | [»] | |
4U51 | X-ray | 3.20 | D7/d7 | 2-82 | [»] | |
4U52 | X-ray | 3.00 | D7/d7 | 2-82 | [»] | |
4U53 | X-ray | 3.30 | D7/d7 | 2-82 | [»] | |
4U55 | X-ray | 3.20 | D7/d7 | 2-82 | [»] | |
4U56 | X-ray | 3.45 | D7/d7 | 2-82 | [»] | |
4U6F | X-ray | 3.10 | D7/d7 | 2-82 | [»] | |
4V6I | electron microscopy | 8.80 | AX | 1-82 | [»] | |
4V88 | X-ray | 3.00 | Ab/Cb | 1-82 | [»] | |
4V8Y | electron microscopy | 4.30 | A1 | 1-82 | [»] | |
4V8Z | electron microscopy | 6.60 | A1 | 1-82 | [»] | |
4V92 | electron microscopy | 3.70 | b | 2-82 | [»] | |
5DAT | X-ray | 3.15 | D7/d7 | 2-82 | [»] | |
5DC3 | X-ray | 3.25 | D7/d7 | 2-82 | [»] | |
5DGE | X-ray | 3.45 | D7/d7 | 2-82 | [»] | |
5DGF | X-ray | 3.30 | D7/d7 | 2-82 | [»] | |
5DGV | X-ray | 3.10 | D7/d7 | 2-82 | [»] | |
5FCI | X-ray | 3.40 | D7/d7 | 2-82 | [»] | |
5FCJ | X-ray | 3.10 | D7/d7 | 2-82 | [»] | |
5I4L | X-ray | 3.10 | D7/d7 | 2-82 | [»] | |
5JUO | electron microscopy | 4.00 | YB | 1-82 | [»] | |
5JUP | electron microscopy | 3.50 | YB | 1-82 | [»] | |
5JUS | electron microscopy | 4.20 | YB | 1-82 | [»] | |
5JUT | electron microscopy | 4.00 | YB | 1-82 | [»] | |
5JUU | electron microscopy | 4.00 | YB | 1-82 | [»] | |
5LL6 | electron microscopy | 3.90 | f | 1-82 | [»] | |
5LYB | X-ray | 3.25 | D7/d7 | 2-82 | [»] | |
5M1J | electron microscopy | 3.30 | b2 | 2-82 | [»] | |
5MC6 | electron microscopy | 3.80 | f | 1-82 | [»] | |
5MEI | X-ray | 3.50 | c/d7 | 2-82 | [»] | |
5NDG | X-ray | 3.70 | D7/d7 | 2-82 | [»] | |
5NDV | X-ray | 3.30 | D7/d7 | 2-82 | [»] | |
5NDW | X-ray | 3.70 | D7/d7 | 2-82 | [»] | |
5OBM | X-ray | 3.40 | D7/d7 | 2-82 | [»] | |
5ON6 | X-ray | 3.10 | c/d7 | 2-82 | [»] | |
5TBW | X-ray | 3.00 | c/d7 | 2-82 | [»] | |
5TGA | X-ray | 3.30 | D7/d7 | 2-82 | [»] | |
5TGM | X-ray | 3.50 | D7/d7 | 2-82 | [»] | |
5WYJ | electron microscopy | 8.70 | Sc | 1-82 | [»] | |
5WYK | electron microscopy | 4.50 | Sc | 1-82 | [»] | |
6EML | electron microscopy | 3.60 | f | 1-82 | [»] | |
6FAI | electron microscopy | 3.40 | b | 1-82 | [»] | |
6GQ1 | electron microscopy | 4.40 | AR | 2-82 | [»] | |
6GQB | electron microscopy | 3.90 | AR | 2-82 | [»] | |
6GQV | electron microscopy | 4.00 | AR | 2-82 | [»] | |
6HHQ | X-ray | 3.10 | c/d7 | 1-82 | [»] | |
6I7O | electron microscopy | 5.30 | f/fb | 2-82 | [»] | |
6KE6 | electron microscopy | 3.40 | Sc | 1-82 | [»] | |
6LQP | electron microscopy | 3.20 | Sc | 1-82 | [»] | |
6LQQ | electron microscopy | 4.10 | Sc | 1-82 | [»] | |
6LQR | electron microscopy | 8.60 | Sc | 1-82 | [»] | |
6LQS | electron microscopy | 3.80 | Sc | 1-82 | [»] | |
6LQT | electron microscopy | 4.90 | Sc | 1-82 | [»] | |
6Q8Y | electron microscopy | 3.10 | f | 2-82 | [»] | |
6RBD | electron microscopy | 3.47 | b | 1-82 | [»] | |
6RBE | electron microscopy | 3.80 | b | 1-82 | [»] | |
6S47 | electron microscopy | 3.28 | Bc | 2-82 | [»] | |
6SNT | electron microscopy | 2.80 | b | 1-82 | [»] | |
6SV4 | electron microscopy | 3.30 | f/fb/fc | 1-82 | [»] | |
6T4Q | electron microscopy | 2.60 | Sb | 2-82 | [»] | |
6T7I | electron microscopy | 3.20 | Sb | 1-82 | [»] | |
6T7T | electron microscopy | 3.10 | Sb | 1-82 | [»] | |
6T83 | electron microscopy | 4.00 | 2/bb | 1-82 | [»] | |
6TB3 | electron microscopy | 2.80 | f | 2-82 | [»] | |
6TNU | electron microscopy | 3.10 | f | 2-82 | [»] | |
6WDR | electron microscopy | 3.70 | b | 2-82 | [»] | |
6WOO | electron microscopy | 2.90 | bb | 2-82 | [»] | |
6XIQ | electron microscopy | 4.20 | AR | 1-82 | [»] | |
6XIR | electron microscopy | 3.20 | AR | 1-82 | [»] | |
6Y7C | electron microscopy | 3.80 | b | 1-82 | [»] | |
6Z6J | electron microscopy | 3.40 | Sb | 1-82 | [»] | |
6Z6K | electron microscopy | 3.40 | Sb | 1-82 | [»] | |
6ZCE | electron microscopy | 5.30 | c | 1-82 | [»] | |
6ZQA | electron microscopy | 4.40 | Db | 1-82 | [»] | |
6ZQB | electron microscopy | 3.90 | Db | 1-82 | [»] | |
6ZQC | electron microscopy | 3.80 | Db | 1-82 | [»] | |
6ZQD | electron microscopy | 3.80 | Db | 1-82 | [»] | |
6ZQE | electron microscopy | 7.10 | Db | 1-82 | [»] | |
6ZQF | electron microscopy | 4.90 | Db | 1-82 | [»] | |
6ZQG | electron microscopy | 3.50 | Db | 1-82 | [»] | |
6ZU9 | electron microscopy | 6.20 | f | 1-82 | [»] | |
6ZVI | electron microscopy | 3.00 | L | 2-82 | [»] | |
7A1G | electron microscopy | 3.00 | f | 2-82 | [»] | |
7AJT | electron microscopy | 4.60 | Db | 1-82 | [»] | |
7AJU | electron microscopy | 3.80 | Db | 1-82 | [»] | |
7B7D | electron microscopy | 3.30 | f | 2-82 | [»] | |
7D4I | electron microscopy | 4.00 | Sc | 1-82 | [»] | |
7D5T | electron microscopy | 6.00 | Sc | 1-82 | [»] | |
7D63 | electron microscopy | 12.30 | Sc | 1-82 | [»] | |
7NRC | electron microscopy | 3.90 | Sf | 2-82 | [»] | |
7NRD | electron microscopy | 4.36 | Sf | 2-82 | [»] | |
7OSA | X-ray | 3.00 | eS27 | 1-82 | [»] | |
7OSM | X-ray | 3.00 | eS27 | 1-82 | [»] | |
7RR5 | electron microscopy | 3.23 | Sb | 1-82 | [»] | |
AlphaFoldDBi | P35997 | |||||
SMRi | P35997 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33981, 713 interactors |
IntActi | P35997, 11 interactors |
MINTi | P35997 |
STRINGi | 4932.YKL156W |
PTM databases
iPTMneti | P35997 |
Proteomic databases
MaxQBi | P35997 |
PaxDbi | P35997 |
PRIDEi | P35997 |
TopDownProteomicsi | P35997 |
Genome annotation databases
EnsemblFungii | YKL156W_mRNA; YKL156W; YKL156W |
GeneIDi | 853700 |
KEGGi | sce:YKL156W |
Organism-specific databases
SGDi | S000001639, RPS27A |
VEuPathDBi | FungiDB:YKL156W |
Phylogenomic databases
eggNOGi | KOG1779, Eukaryota |
GeneTreei | ENSGT00940000167938 |
HOGENOMi | CLU_130128_3_0_1 |
InParanoidi | P35997 |
OMAi | LHPAIDF |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-1799339, SRP-dependent cotranslational protein targeting to membrane R-SCE-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-SCE-72689, Formation of a pool of free 40S subunits R-SCE-72695, Formation of the ternary complex, and subsequently, the 43S complex R-SCE-72702, Ribosomal scanning and start codon recognition R-SCE-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
PROi | PR:P35997 |
RNActi | P35997, protein |
Family and domain databases
Gene3Di | 2.20.25.100, 1 hit |
HAMAPi | MF_00371, Ribosomal_S27e, 1 hit |
InterProi | View protein in InterPro IPR000592, Ribosomal_S27 IPR023407, Ribosomal_S27_Zn-bd_dom_sf IPR011332, Ribosomal_zn-bd |
PANTHERi | PTHR11594, PTHR11594, 1 hit |
Pfami | View protein in Pfam PF01667, Ribosomal_S27e, 1 hit |
SUPFAMi | SSF57829, SSF57829, 1 hit |
PROSITEi | View protein in PROSITE PS01168, RIBOSOMAL_S27E, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RS27A_YEAST | |
Accessioni | P35997Primary (citable) accession number: P35997 Secondary accession number(s): D6VX42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - Ribosomal proteins
Ribosomal proteins families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families