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UniProtKB - P35862 (CH603_BRADU)
Protein
Chaperonin GroEL 3
Gene
groEL3
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Functioni
Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.
UniRule annotationCatalytic activityi
- ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.UniRule annotation EC:5.6.1.7
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 51 | ATPUniRule annotation | 1 | |
Binding sitei | 415 | ATP; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 496 | ATPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 33 | ATPUniRule annotation | 4 | |
Nucleotide bindingi | 87 – 91 | ATPUniRule annotation | 5 |
GO - Molecular functioni
- ATP binding Source: GO_Central
- ATP hydrolysis activity Source: InterPro
- isomerase activity Source: UniProtKB-KW
- unfolded protein binding Source: GO_Central
GO - Biological processi
- chaperone cofactor-dependent protein refolding Source: GO_Central
- protein folding Source: GO_Central
- protein refolding Source: GO_Central
- response to heat Source: GO_Central
Keywordsi
Molecular function | Chaperone, Isomerase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Chaperonin GroEL 3UniRule annotation (EC:5.6.1.7UniRule annotation)Alternative name(s): 60 kDa chaperonin 3UniRule annotation Chaperonin-60 3UniRule annotation Short name: Cpn60 3UniRule annotation |
Gene namesi | Name:groEL3UniRule annotation Synonyms:groL3UniRule annotation Ordered Locus Names:bll2059 |
Organismi | Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) |
Taxonomic identifieri | 224911 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Hyphomicrobiales › Bradyrhizobiaceae › Bradyrhizobium › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- GroEL-GroES complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000063296 | 2 – 546 | Chaperonin GroEL 3Add BLAST | 545 |
Proteomic databases
PRIDEi | P35862 |
Expressioni
Inductioni
By NifA.
Interactioni
Subunit structurei
Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back.
Interacts with the co-chaperonin GroES.
UniRule annotationGO - Molecular functioni
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 224911.27350313 |
Family & Domainsi
Sequence similaritiesi
Belongs to the chaperonin (HSP60) family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0459, Bacteria |
HOGENOMi | CLU_016503_3_0_5 |
InParanoidi | P35862 |
OMAi | LKDQHMN |
PhylomeDBi | P35862 |
Family and domain databases
CDDi | cd03344, GroEL, 1 hit |
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
HAMAPi | MF_00600, CH60, 1 hit |
InterProi | View protein in InterPro IPR018370, Chaperonin_Cpn60_CS IPR001844, Chaprnin_Cpn60 IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00298, CHAPERONIN60 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02348, GroEL, 1 hit |
PROSITEi | View protein in PROSITE PS00296, CHAPERONINS_CPN60, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P35862-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAKEVKFGV NARDRMLRGV DILANAVQVT LGPKGRNVVL DKSFGAPRIT
60 70 80 90 100
KDGVAVAKEI ELDDKFENMG AQMVREVASK AADAAGDGTT TATVLAAAIV
110 120 130 140 150
REGAKSVAAG MNPMDLKRGI DLAVEAVVAD LQKNSKKVTS NDEIAQVGAI
160 170 180 190 200
SANGDQEIGK FLADAVKKVG NEGVITVEEA KSLETELDVV EGMQFDRGYI
210 220 230 240 250
SPYFVTNADK MRVEMDDAYI LINEKKLSSL NELLPLLEAV VQTGKPLVIV
260 270 280 290 300
AEDVEGEALA TLVVNRLRGG LKVAAVKAPG FGDRRKAMLQ DIAILTGGQA
310 320 330 340 350
ISEDLGIKLE NVTLNMLGRA KKVMIDKENT TIVNGAGKKA DIEARVAQIK
360 370 380 390 400
AQIEETTSDY DREKLQERLA KLAGGVAVIR VGGATEVEVK ERKDRVDDAM
410 420 430 440 450
HATRAAVEEG IVPGGGVALL RASEQLKGLR TENDDQKTGV EIVRKALSWP
460 470 480 490 500
ARQIAINAGE DGSIVVGKVL DNEQYSFGFD AQTGEYSNLV SKGIIDPAKV
510 520 530 540
VRIAVQNASS VAGLLITTEA MVAELPKKAT AGPAMPAAPG MGGMDF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22603 Genomic DNA Translation: CAA80316.1 AF322013 Genomic DNA Translation: AAG61029.1 BA000040 Genomic DNA Translation: BAC47324.1 |
PIRi | S35311 |
RefSeqi | NP_768699.1, NC_004463.1 WP_011084855.1, NZ_CP011360.1 |
Genome annotation databases
EnsemblBacteriai | BAC47324; BAC47324; BAC47324 |
GeneIDi | 64021824 |
KEGGi | bja:bll2059 |
PATRICi | fig|224911.44.peg.1564 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22603 Genomic DNA Translation: CAA80316.1 AF322013 Genomic DNA Translation: AAG61029.1 BA000040 Genomic DNA Translation: BAC47324.1 |
PIRi | S35311 |
RefSeqi | NP_768699.1, NC_004463.1 WP_011084855.1, NZ_CP011360.1 |
3D structure databases
SMRi | P35862 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224911.27350313 |
Proteomic databases
PRIDEi | P35862 |
Genome annotation databases
EnsemblBacteriai | BAC47324; BAC47324; BAC47324 |
GeneIDi | 64021824 |
KEGGi | bja:bll2059 |
PATRICi | fig|224911.44.peg.1564 |
Phylogenomic databases
eggNOGi | COG0459, Bacteria |
HOGENOMi | CLU_016503_3_0_5 |
InParanoidi | P35862 |
OMAi | LKDQHMN |
PhylomeDBi | P35862 |
Family and domain databases
CDDi | cd03344, GroEL, 1 hit |
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
HAMAPi | MF_00600, CH60, 1 hit |
InterProi | View protein in InterPro IPR018370, Chaperonin_Cpn60_CS IPR001844, Chaprnin_Cpn60 IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00298, CHAPERONIN60 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02348, GroEL, 1 hit |
PROSITEi | View protein in PROSITE PS00296, CHAPERONINS_CPN60, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CH603_BRADU | |
Accessioni | P35862Primary (citable) accession number: P35862 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 123 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families