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Protein

Choline kinase alpha

Gene

CHKA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.1 Publication

Catalytic activityi

ATP + choline = ADP + phosphocholine.1 Publication
ATP + ethanolamine = ADP + O-phosphoethanolamine.1 Publication

Kineticsi

  1. KM=0.2 mM for choline2 Publications
  2. KM=0.4 mM for ATP2 Publications
  3. KM=12 mM for ethanolamine2 Publications

    Pathwayi: phosphatidylcholine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes phosphocholine from choline.
    Proteins known to be involved in this subpathway in this organism are:
    1. Choline kinase alpha (CHKA)
    This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphocholine from choline, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

    Pathwayi: phosphatidylethanolamine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Choline kinase alpha (CHKA), Ethanolamine kinase 1 (ETNK1), Ethanolamine kinase 2 (ETNK2), Choline/ethanolamine kinase (CHKB)
    2. Ethanolamine-phosphate cytidylyltransferase (PCYT2)
    3. Ethanolaminephosphotransferase 1 (SELENOI), Choline/ethanolaminephosphotransferase 1 (CEPT1)
    This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei146ATP1
    Binding sitei308ATPCurated1
    Binding sitei330ATP1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi117 – 123ATP7
    Nucleotide bindingi207 – 213ATP7

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS03334-MONOMER
    BRENDAi2.7.1.32 2681
    ReactomeiR-HSA-1483191 Synthesis of PC
    R-HSA-1483213 Synthesis of PE
    SABIO-RKiP35790
    UniPathwayi
    UPA00558;UER00741

    UPA00753;UER00737

    Chemistry databases

    SwissLipidsiSLP:000001746 [P35790-1]

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline kinase alpha (EC:2.7.1.32)
    Short name:
    CK
    Alternative name(s):
    CHETK-alpha
    Ethanolamine kinase (EC:2.7.1.82)
    Short name:
    EK
    Gene namesi
    Name:CHKA
    Synonyms:CHK, CKI
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 11

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000110721.11
    HGNCiHGNC:1937 CHKA
    MIMi118491 gene
    neXtProtiNX_P35790

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi1119
    OpenTargetsiENSG00000110721
    PharmGKBiPA26468

    Chemistry databases

    ChEMBLiCHEMBL3117
    DrugBankiDB00122 Choline

    Polymorphism and mutation databases

    BioMutaiCHKA
    DMDMi226694197

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002062191 – 457Choline kinase alphaAdd BLAST457

    Proteomic databases

    EPDiP35790
    MaxQBiP35790
    PaxDbiP35790
    PeptideAtlasiP35790
    PRIDEiP35790
    ProteomicsDBi55154
    55155 [P35790-2]

    PTM databases

    iPTMnetiP35790
    PhosphoSitePlusiP35790

    Expressioni

    Gene expression databases

    BgeeiENSG00000110721 Expressed in 204 organ(s), highest expression level in testis
    CleanExiHS_CHKA
    ExpressionAtlasiP35790 baseline and differential
    GenevisibleiP35790 HS

    Organism-specific databases

    HPAiCAB072803
    HPA024153

    Interactioni

    Subunit structurei

    Heterodimer with CHKB (By similarity). Homodimer.By similarity2 Publications

    GO - Molecular functioni

    Protein-protein interaction databases

    BioGridi107543, 5 interactors
    IntActiP35790, 1 interactor
    STRINGi9606.ENSP00000265689

    Chemistry databases

    BindingDBiP35790

    Structurei

    Secondary structure

    1457
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP35790
    SMRiP35790
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35790

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni119 – 121Substrate binding3

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi50 – 85Pro-richAdd BLAST36

    Sequence similaritiesi

    Belongs to the choline/ethanolamine kinase family.Curated

    Phylogenomic databases

    eggNOGiKOG2686 Eukaryota
    COG0510 LUCA
    GeneTreeiENSGT00530000062991
    HOGENOMiHOG000041274
    HOVERGENiHBG050943
    InParanoidiP35790
    KOiK14156
    OMAiRAYLWCK
    OrthoDBiEOG091G06UK
    PhylomeDBiP35790
    TreeFamiTF313549

    Family and domain databases

    InterProiView protein in InterPro
    IPR011009 Kinase-like_dom_sf
    SUPFAMiSSF56112 SSF56112, 1 hit

    Sequences (2+)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: P35790-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG
    60 70 80 90 100
    QQPPLALPPP PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP
    110 120 130 140 150
    GAWRGLREDE FHISVIRGGL SNMLFQCSLP DTTATLGDEP RKVLLRLYGA
    160 170 180 190 200
    ILQMRSCNKE GSEQAQKENE FQGAEAMVLE SVMFAILAER SLGPKLYGIF
    210 220 230 240 250
    PQGRLEQFIP SRRLDTEELS LPDISAEIAE KMATFHGMKM PFNKEPKWLF
    260 270 280 290 300
    GTMEKYLKEV LRIKFTEESR IKKLHKLLSY NLPLELENLR SLLESTPSPV
    310 320 330 340 350
    VFCHNDCQEG NILLLEGREN SEKQKLMLID FEYSSYNYRG FDIGNHFCEW
    360 370 380 390 400
    MYDYSYEKYP FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI
    410 420 430 440 450
    IKEEMLLEVN RFALASHFLW GLWSIVQAKI SSIEFGYMDY AQARFDAYFH

    QKRKLGV
    Length:457
    Mass (Da):52,249
    Last modified:April 14, 2009 - v3
    Checksum:i65F177ABE1AA3A12
    GO
    Isoform 2 (identifier: P35790-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         155-172: Missing.

    Show »
    Length:439
    Mass (Da):50,155
    Checksum:i73507D716F15F9E4
    GO

    Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    H0YD02H0YD02_HUMAN
    Choline kinase alpha
    CHKA
    164Annotation score:
    E9PM06E9PM06_HUMAN
    Choline kinase alpha
    CHKA
    156Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti49 – 54GGQQPP → APTAA in BAA01547 (PubMed:1618328).Curated6
    Sequence conflicti87T → A in BAA01547 (PubMed:1618328).Curated1
    Sequence conflicti154M → V in BAA01547 (PubMed:1618328).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_054863220S → G1 PublicationCorresponds to variant dbSNP:rs17853641Ensembl.1
    Natural variantiVAR_054864422L → Q1 PublicationCorresponds to variant dbSNP:rs17853642Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_009683155 – 172Missing in isoform 2. 1 PublicationAdd BLAST18

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D10704 mRNA Translation: BAA01547.1
    AP002807 Genomic DNA No translation available.
    AP002992 Genomic DNA No translation available.
    BC036471 mRNA Translation: AAH36471.1
    CCDSiCCDS8178.1 [P35790-1]
    CCDS8179.1 [P35790-2]
    PIRiS23104
    RefSeqiNP_001268.2, NM_001277.2 [P35790-1]
    NP_997634.1, NM_212469.1 [P35790-2]
    UniGeneiHs.77221

    Genome annotation databases

    EnsembliENST00000265689; ENSP00000265689; ENSG00000110721 [P35790-1]
    ENST00000356135; ENSP00000348454; ENSG00000110721 [P35790-2]
    GeneIDi1119
    KEGGihsa:1119
    UCSCiuc001onj.4 human [P35790-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D10704 mRNA Translation: BAA01547.1
    AP002807 Genomic DNA No translation available.
    AP002992 Genomic DNA No translation available.
    BC036471 mRNA Translation: AAH36471.1
    CCDSiCCDS8178.1 [P35790-1]
    CCDS8179.1 [P35790-2]
    PIRiS23104
    RefSeqiNP_001268.2, NM_001277.2 [P35790-1]
    NP_997634.1, NM_212469.1 [P35790-2]
    UniGeneiHs.77221

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2CKOX-ray2.15A/B50-457[»]
    2CKPX-ray3.10A/B50-457[»]
    2CKQX-ray2.40A/B50-457[»]
    2I7QX-ray1.90A75-457[»]
    3F2RX-ray2.35A/B75-457[»]
    3G15X-ray1.70A/B75-457[»]
    3ZM9X-ray1.90A/B75-457[»]
    4BR3X-ray2.20A/B75-457[»]
    4CG8X-ray1.75A75-457[»]
    4CG9X-ray1.83A75-457[»]
    4CGAX-ray1.74A75-457[»]
    4DA5X-ray2.40A/B1-457[»]
    5AFVX-ray2.25A/B80-457[»]
    5EQEX-ray2.40A/B75-457[»]
    5EQPX-ray2.35A/B75-457[»]
    5EQYX-ray2.50A/B75-457[»]
    5FTGX-ray1.45A80-457[»]
    5FUTX-ray1.60A80-457[»]
    5W6OX-ray2.35A/B80-457[»]
    ProteinModelPortaliP35790
    SMRiP35790
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi107543, 5 interactors
    IntActiP35790, 1 interactor
    STRINGi9606.ENSP00000265689

    Chemistry databases

    BindingDBiP35790
    ChEMBLiCHEMBL3117
    DrugBankiDB00122 Choline
    SwissLipidsiSLP:000001746 [P35790-1]

    PTM databases

    iPTMnetiP35790
    PhosphoSitePlusiP35790

    Polymorphism and mutation databases

    BioMutaiCHKA
    DMDMi226694197

    Proteomic databases

    EPDiP35790
    MaxQBiP35790
    PaxDbiP35790
    PeptideAtlasiP35790
    PRIDEiP35790
    ProteomicsDBi55154
    55155 [P35790-2]

    Protocols and materials databases

    DNASUi1119
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000265689; ENSP00000265689; ENSG00000110721 [P35790-1]
    ENST00000356135; ENSP00000348454; ENSG00000110721 [P35790-2]
    GeneIDi1119
    KEGGihsa:1119
    UCSCiuc001onj.4 human [P35790-1]

    Organism-specific databases

    CTDi1119
    DisGeNETi1119
    EuPathDBiHostDB:ENSG00000110721.11
    GeneCardsiCHKA
    H-InvDBiHIX0017419
    HGNCiHGNC:1937 CHKA
    HPAiCAB072803
    HPA024153
    MIMi118491 gene
    neXtProtiNX_P35790
    OpenTargetsiENSG00000110721
    PharmGKBiPA26468
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2686 Eukaryota
    COG0510 LUCA
    GeneTreeiENSGT00530000062991
    HOGENOMiHOG000041274
    HOVERGENiHBG050943
    InParanoidiP35790
    KOiK14156
    OMAiRAYLWCK
    OrthoDBiEOG091G06UK
    PhylomeDBiP35790
    TreeFamiTF313549

    Enzyme and pathway databases

    UniPathwayi
    UPA00558;UER00741

    UPA00753;UER00737

    BioCyciMetaCyc:HS03334-MONOMER
    BRENDAi2.7.1.32 2681
    ReactomeiR-HSA-1483191 Synthesis of PC
    R-HSA-1483213 Synthesis of PE
    SABIO-RKiP35790

    Miscellaneous databases

    ChiTaRSiCHKA human
    EvolutionaryTraceiP35790
    GeneWikiiCHKA
    GenomeRNAii1119
    PROiPR:P35790
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000110721 Expressed in 204 organ(s), highest expression level in testis
    CleanExiHS_CHKA
    ExpressionAtlasiP35790 baseline and differential
    GenevisibleiP35790 HS

    Family and domain databases

    InterProiView protein in InterPro
    IPR011009 Kinase-like_dom_sf
    SUPFAMiSSF56112 SSF56112, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCHKA_HUMAN
    AccessioniPrimary (citable) accession number: P35790
    Secondary accession number(s): Q8NE29
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: April 14, 2009
    Last modified: September 12, 2018
    This is version 163 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    7. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
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