UniProtKB - P35762 (CD81_MOUSE)
CD81 antigen
Cd81
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 116 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 144 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 148 | Important for interaction with integrinBy similarity | 1 | |
Binding sitei | 219 | CholesterolBy similarity | 1 |
GO - Molecular functioni
- cholesterol binding Source: UniProtKB
- integrin binding Source: UniProtKB
- MHC class II protein binding Source: MGI
- transferrin receptor binding Source: MGI
- virus receptor activity Source: MGI
GO - Biological processi
- activation of MAPK activity Source: UniProtKB
- CD4-positive, alpha-beta T cell costimulation Source: UniProtKB
- cellular response to low-density lipoprotein particle stimulus Source: UniProtKB
- humoral immune response mediated by circulating immunoglobulin Source: UniProtKB
- immunological synapse formation Source: UniProtKB
- macrophage fusion Source: UniProtKB
- myoblast fusion involved in skeletal muscle regeneration Source: UniProtKB
- osteoclast fusion Source: UniProtKB
- positive regulation of 1-phosphatidylinositol 4-kinase activity Source: UniProtKB
- positive regulation of adaptive immune memory response Source: MGI
- positive regulation of B cell activation Source: UniProtKB
- positive regulation of B cell proliferation Source: MGI
- positive regulation of B cell receptor signaling pathway Source: UniProtKB
- positive regulation of CD4-positive, alpha-beta T cell proliferation Source: UniProtKB
- positive regulation of cell growth Source: UniProtKB
- positive regulation of cell population proliferation Source: MGI
- positive regulation of inflammatory response to antigenic stimulus Source: UniProtKB
- positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
- positive regulation of protein catabolic process in the vacuole Source: MGI
- positive regulation of protein exit from endoplasmic reticulum Source: UniProtKB
- positive regulation of receptor clustering Source: UniProtKB
- positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: UniProtKB
- positive regulation of T cell receptor signaling pathway Source: UniProtKB
- positive regulation of T-helper 2 cell cytokine production Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein localization Source: UniProtKB
- protein localization to lysosome Source: MGI
- protein localization to plasma membrane Source: UniProtKB
- receptor internalization Source: UniProtKB
- regulation of cell motility Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of macrophage migration Source: UniProtKB
- regulation of protein stability Source: MGI
Keywordsi
Biological process | Adaptive immunity, Immunity |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-977606, Regulation of Complement cascade |
Names & Taxonomyi
Protein namesi | Recommended name: CD81 antigenAlternative name(s): 26 kDa cell surface protein TAPA-1 Target of the antiproliferative antibody 11 Publication CD_antigen: CD81 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096398, Cd81 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
- Basolateral cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.By similarity
Extracellular region or secreted
- extracellular exosome Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
- basal plasma membrane Source: MGI
- basolateral plasma membrane Source: MGI
- immunological synapse Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- tetraspanin-enriched microdomain Source: UniProtKB
Other locations
- integral component of membrane Source: MGI
- membrane Source: UniProtKB
- vesicle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 13 – 33 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 34 – 63 | ExtracellularCuratedAdd BLAST | 30 | |
Transmembranei | 64 – 84 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 85 – 89 | CytoplasmicCurated | 5 | |
Transmembranei | 90 – 112 | HelicalBy similarityAdd BLAST | 23 | |
Topological domaini | 113 – 201 | ExtracellularCuratedAdd BLAST | 89 | |
Transmembranei | 202 – 224 | HelicalBy similarityAdd BLAST | 23 | |
Topological domaini | 225 – 236 | CytoplasmicCuratedAdd BLAST | 12 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219222 | 1 – 236 | CD81 antigenAdd BLAST | 236 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 156 ↔ 190 | Combined sources1 Publication | ||
Disulfide bondi | 157 ↔ 175 | Combined sources1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | P35762 |
jPOSTi | P35762 |
PaxDbi | P35762 |
PeptideAtlasi | P35762 |
PRIDEi | P35762 |
PTM databases
iPTMneti | P35762 |
PhosphoSitePlusi | P35762 |
SwissPalmi | P35762 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000037706, Expressed in ventral horn of spinal cord and 333 other tissues |
Genevisiblei | P35762, MM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B cells.
Interacts (via the second extracellular domain) with CD19; this interaction is initiated early during biosynthesis in the ER and enables trafficking of only properly folded CD19 (By similarity).
Part of a complex that includes MHC class II/HLA-DR molecules and IFITM1.
Interacts with IFITM1 (By similarity).
Interacts with IFITM2 and IFITM3 (PubMed:16395393). Part of integrin-tetraspanin complex composed of CD9, CD81, beta-1 and beta-2 integrins in the membrane of monocyte/macrophages (By similarity).
Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (By similarity).
Interacts with CD247/CD3 zeta, ICAM1 and CD9 at the immune synapse on T cell membrane (By similarity).
Part of a GPCR-tetraspanin complex consisting at least of ADGRG1, CD81, possibly CD9, and GNA11 in which CD81 enhances the association of ADGRG1 with GNA11 (By similarity).
Part of a complex composed of CD9, CD81, PTGFRN and IGSF8 (PubMed:23575678).
Interacts directly with IGSF8 (PubMed:11673522).
Interacts with CD53 and SCIMP (By similarity).
Interacts with SAMHD1 (via its C-terminus) (By similarity).
Interacts with glypican GPC3 and with the transcriptional repressor HHEX; binding to GPC3 decreases the availability of free CD81 for binding to HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed:23665349).
Interacts with CLDN1 (By similarity).
Interacts with CLDN6 and CLDN9 (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- integrin binding Source: UniProtKB
- MHC class II protein binding Source: MGI
- transferrin receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198613, 18 interactors |
IntActi | P35762, 4 interactors |
MINTi | P35762 |
STRINGi | 10090.ENSMUSP00000043768 |
Miscellaneous databases
RNActi | P35762, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P35762 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3882, Eukaryota |
GeneTreei | ENSGT00940000158805 |
HOGENOMi | CLU_055524_10_0_1 |
InParanoidi | P35762 |
OMAi | HETLSCC |
OrthoDBi | 1205716at2759 |
TreeFami | TF352895 |
Family and domain databases
Gene3Di | 1.10.1450.10, 1 hit |
InterProi | View protein in InterPro IPR000301, Tetraspanin IPR018499, Tetraspanin/Peripherin IPR018503, Tetraspanin_CS IPR008952, Tetraspanin_EC2_sf |
Pfami | View protein in Pfam PF00335, Tetraspanin, 1 hit |
PIRSFi | PIRSF002419, Tetraspanin, 1 hit |
PRINTSi | PR00259, TMFOUR |
SUPFAMi | SSF48652, SSF48652, 1 hit |
PROSITEi | View protein in PROSITE PS00421, TM4_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTSLLYLELG
60 70 80 90 100
NKPAPNTFYV GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI
110 120 130 140 150
LFACEVAAGI WGFVNKDQIA KDVKQFYDQA LQQAVMDDDA NNAKAVVKTF
160 170 180 190 200
HETLNCCGSN ALTTLTTTIL RNSLCPSGGN ILTPLLQQDC HQKIDELFSG
210 220 230
KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LJL0 | A0A140LJL0_MOUSE | CD81 antigen | Cd81 | 121 | Annotation score: | ||
A0A140LHT1 | A0A140LHT1_MOUSE | CD81 antigen | Cd81 | 68 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 173 | S → T in AAB19417 (PubMed:1650385).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S45012 , S44957, S44966, S45001, S45008, S45010 Genomic DNA Translation: AAB19417.1 AJ251835 Genomic DNA Translation: CAB94774.1 AK166521 mRNA Translation: BAE38825.1 AK170741 mRNA Translation: BAE41994.1 AC015800 Genomic DNA No translation available. CH466531 Genomic DNA Translation: EDL18195.1 BC011433 mRNA Translation: AAH11433.1 X59047 mRNA No translation available. |
CCDSi | CCDS22038.1 |
PIRi | A46472 |
RefSeqi | NP_598416.1, NM_133655.2 |
Genome annotation databases
Ensembli | ENSMUST00000037941; ENSMUSP00000043768; ENSMUSG00000037706 |
GeneIDi | 12520 |
KEGGi | mmu:12520 |
UCSCi | uc009kou.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S45012 , S44957, S44966, S45001, S45008, S45010 Genomic DNA Translation: AAB19417.1 AJ251835 Genomic DNA Translation: CAB94774.1 AK166521 mRNA Translation: BAE38825.1 AK170741 mRNA Translation: BAE41994.1 AC015800 Genomic DNA No translation available. CH466531 Genomic DNA Translation: EDL18195.1 BC011433 mRNA Translation: AAH11433.1 X59047 mRNA No translation available. |
CCDSi | CCDS22038.1 |
PIRi | A46472 |
RefSeqi | NP_598416.1, NM_133655.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3X0F | X-ray | 1.47 | A/B | 113-202 | [»] | |
SMRi | P35762 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198613, 18 interactors |
IntActi | P35762, 4 interactors |
MINTi | P35762 |
STRINGi | 10090.ENSMUSP00000043768 |
PTM databases
iPTMneti | P35762 |
PhosphoSitePlusi | P35762 |
SwissPalmi | P35762 |
Proteomic databases
EPDi | P35762 |
jPOSTi | P35762 |
PaxDbi | P35762 |
PeptideAtlasi | P35762 |
PRIDEi | P35762 |
Protocols and materials databases
Antibodypediai | 1570, 1253 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000037941; ENSMUSP00000043768; ENSMUSG00000037706 |
GeneIDi | 12520 |
KEGGi | mmu:12520 |
UCSCi | uc009kou.2, mouse |
Organism-specific databases
CTDi | 975 |
MGIi | MGI:1096398, Cd81 |
Phylogenomic databases
eggNOGi | KOG3882, Eukaryota |
GeneTreei | ENSGT00940000158805 |
HOGENOMi | CLU_055524_10_0_1 |
InParanoidi | P35762 |
OMAi | HETLSCC |
OrthoDBi | 1205716at2759 |
TreeFami | TF352895 |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-977606, Regulation of Complement cascade |
Miscellaneous databases
BioGRID-ORCSi | 12520, 0 hits in 19 CRISPR screens |
ChiTaRSi | Cd81, mouse |
PROi | PR:P35762 |
RNActi | P35762, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037706, Expressed in ventral horn of spinal cord and 333 other tissues |
Genevisiblei | P35762, MM |
Family and domain databases
Gene3Di | 1.10.1450.10, 1 hit |
InterProi | View protein in InterPro IPR000301, Tetraspanin IPR018499, Tetraspanin/Peripherin IPR018503, Tetraspanin_CS IPR008952, Tetraspanin_EC2_sf |
Pfami | View protein in Pfam PF00335, Tetraspanin, 1 hit |
PIRSFi | PIRSF002419, Tetraspanin, 1 hit |
PRINTSi | PR00259, TMFOUR |
SUPFAMi | SSF48652, SSF48652, 1 hit |
PROSITEi | View protein in PROSITE PS00421, TM4_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CD81_MOUSE | |
Accessioni | P35762Primary (citable) accession number: P35762 Secondary accession number(s): Q91V78 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | October 3, 2012 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families