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UniProtKB - P35750 (CAN1_PIG)
Protein
Calpain-1 catalytic subunit
Gene
CAPN1
Organism
Sus scrofa (Pig)
Status
Functioni
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1.
By similarityCatalytic activityi
- Broad endopeptidase specificity.By similarity EC:3.4.22.52
Cofactori
Ca2+By similarityNote: Binds 4 Ca2+ ions.By similarity
Activity regulationi
Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 109 | Calcium 1Curated | 1 | |
Metal bindingi | 114 | Calcium 1Curated | 1 | |
Active sitei | 115 | By similarity | 1 | |
Active sitei | 272 | By similarity | 1 | |
Active sitei | 296 | By similarity | 1 | |
Metal bindingi | 318 | Calcium 2Curated | 1 | |
Metal bindingi | 323 | Calcium 2Curated | 1 | |
Metal bindingi | 598 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 600 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 602 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 604 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 609 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 628 | Calcium 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 630 | Calcium 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 632 | Calcium 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 634 | Calcium 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 639 | Calcium 4PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium-dependent cysteine-type endopeptidase activity Source: CAFA
- calcium ion binding Source: UniProtKB
GO - Biological processi
- proteolysis Source: CAFA
- regulation of catalytic activity Source: UniProtKB
- self proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.22.52, 6170 |
Reactomei | R-SSC-1474228, Degradation of the extracellular matrix R-SSC-6798695, Neutrophil degranulation R-SSC-6809371, Formation of the cornified envelope |
Protein family/group databases
MEROPSi | C02.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Calpain-1 catalytic subunitCurated (EC:3.4.22.52By similarity)Alternative name(s): Calcium-activated neutral proteinase 1By similarity Short name: CANP 1By similarity Calpain mu-typeBy similarity Calpain-1 large subunitBy similarity Micromolar-calpainBy similarity Short name: muCANPBy similarity |
Gene namesi | Name:CAPN1By similarity |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:86170, CAPN1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: CAFA
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207697 | 1 – 714 | Calpain-1 catalytic subunitAdd BLAST | 714 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 354 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 15 – 16 | Cleavage; for 78 kDa formBy similarity | 2 | |
Sitei | 27 – 28 | Cleavage; for 75 kDa formBy similarity | 2 |
Keywords - PTMi
Autocatalytic cleavage, PhosphoproteinProteomic databases
PaxDbi | P35750 |
PeptideAtlasi | P35750 |
PRIDEi | P35750 |
Expressioni
Gene expression databases
Bgeei | ENSSSCG00000012999, Expressed in blood and 48 other tissues |
ExpressionAtlasi | P35750, baseline and differential |
Genevisiblei | P35750, SS |
Interactioni
Subunit structurei
Forms a heterodimer with a small (regulatory) subunit CAPNS1.
By similarityProtein-protein interaction databases
STRINGi | 9823.ENSSSCP00000013824 |
Chemistry databases
BindingDBi | P35750 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 354 | Calpain catalyticPROSITE-ProRule annotationAdd BLAST | 300 | |
Domaini | 585 – 618 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 615 – 650 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 680 – 714 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 355 – 526 | Domain IIIAdd BLAST | 172 | |
Regioni | 527 – 542 | LinkerAdd BLAST | 16 | |
Regioni | 543 – 713 | Domain IVAdd BLAST | 171 |
Sequence similaritiesi
Belongs to the peptidase C2 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0045, Eukaryota |
GeneTreei | ENSGT00940000159147 |
HOGENOMi | CLU_010982_0_1_1 |
InParanoidi | P35750 |
OMAi | TEWFDLR |
OrthoDBi | 704215at2759 |
TreeFami | TF314748 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029643, CAPN1 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF284, PTHR10183:SF284, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P35750-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEEVITPVY CTGVSAQVQK LRAKELGLGR HENAIKYLGQ DYEQLRAHCL
60 70 80 90 100
QSGSLFRDEA FPPVPQSLGF KELGPNSSKT YGVKWKRPTE LFSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQNGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVVDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGST SEGFEDFTGG VTEWYELRKA PSDLYSIILK ALERGSLLGC
260 270 280 290 300
SIDISSVLDM EAVTFKKLVK GHAYSVTGAK QVNYQGQMVN LIRMRNPWGE
310 320 330 340 350
VEWTGAWSDG SSEWNGVDPY QRDQLRVRME DGEFWMSFRD FLREFTRLEI
360 370 380 390 400
CNLTPDALKS QRVRNWNTTL YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEETDDPEDD YGGRESGCSF VLALMQKHRR RERRFGRDME TIGFAVYEVP
460 470 480 490 500
PELVGQPVHL KRDFFLANAS RARSEQFINL REVSTRFRLP PGEYVVVPST
510 520 530 540 550
FEPNKEGDFV LRFFSEKKAG TQELDDQVQA ILPDEQVLSE EEIDENFKAL
560 570 580 590 600
FRQLAGEDME ISVRELRTIL NRIISKHKDL RTKGFSLESC RSMVNLMDRD
610 620 630 640 650
GNGKLGLVEF NILWNRIRNY LSIFRKFDLD KSGSMSAYEM RMAIESAGFK
660 670 680 690 700
LNKKLFELII TRYSEPDLAV DFDNFVCCLV RLETMFRFFK TLDTDLDGVV
710
TFDLFKWLQL TMFA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5S6GBZ7 | A0A5S6GBZ7_PIG | Calpain-1 catalytic subunit | CAPN1 | 775 | Annotation score: | ||
A0A5S6H4P7 | A0A5S6H4P7_PIG | Calpain-1 catalytic subunit | CAPN1 | 650 | Annotation score: | ||
A0A5G2QYC6 | A0A5G2QYC6_PIG | Calpain-1 catalytic subunit | CAPN1 | 601 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 528 | V → I in AAA65125 (PubMed:8312396).Curated | 1 | |
Sequence conflicti | 531 | I → N in AAA65125 (PubMed:8312396).Curated | 1 | |
Sequence conflicti | 541 | E → G in AAA65125 (PubMed:8312396).Curated | 1 | |
Sequence conflicti | 622 | S → A in AAA65125 (PubMed:8312396).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF263610 mRNA Translation: AAF73444.1 F14611 mRNA Translation: CAA23154.1 U01180 mRNA Translation: AAA65125.1 |
RefSeqi | NP_001335713.1, NM_001348784.1 XP_005660759.1, XM_005660702.2 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF263610 mRNA Translation: AAF73444.1 F14611 mRNA Translation: CAA23154.1 U01180 mRNA Translation: AAA65125.1 |
RefSeqi | NP_001335713.1, NM_001348784.1 XP_005660759.1, XM_005660702.2 |
3D structure databases
AlphaFoldDBi | P35750 |
SMRi | P35750 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000013824 |
Chemistry databases
BindingDBi | P35750 |
ChEMBLi | CHEMBL4062 |
Protein family/group databases
MEROPSi | C02.001 |
Proteomic databases
PaxDbi | P35750 |
PeptideAtlasi | P35750 |
PRIDEi | P35750 |
Genome annotation databases
Organism-specific databases
CTDi | 823 |
VGNCi | VGNC:86170, CAPN1 |
Phylogenomic databases
eggNOGi | KOG0045, Eukaryota |
GeneTreei | ENSGT00940000159147 |
HOGENOMi | CLU_010982_0_1_1 |
InParanoidi | P35750 |
OMAi | TEWFDLR |
OrthoDBi | 704215at2759 |
TreeFami | TF314748 |
Enzyme and pathway databases
BRENDAi | 3.4.22.52, 6170 |
Reactomei | R-SSC-1474228, Degradation of the extracellular matrix R-SSC-6798695, Neutrophil degranulation R-SSC-6809371, Formation of the cornified envelope |
Miscellaneous databases
PROi | PR:P35750 |
Gene expression databases
Bgeei | ENSSSCG00000012999, Expressed in blood and 48 other tissues |
ExpressionAtlasi | P35750, baseline and differential |
Genevisiblei | P35750, SS |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029643, CAPN1 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF284, PTHR10183:SF284, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAN1_PIG | |
Accessioni | P35750Primary (citable) accession number: P35750 Secondary accession number(s): Q29600, Q9N0M6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | January 11, 2001 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families