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UniProtKB - P35669 (GSHB_SCHPO)
Protein
Glutathione synthetase large chain
Gene
gsa1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Catalytic activityi
- EC:6.3.2.3
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
: glutathione biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate. This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 128 | SubstrateBy similarity | 1 | |
Metal bindingi | 146 | MagnesiumBy similarity | 1 | |
Binding sitei | 146 | ATPBy similarity | 1 | |
Metal bindingi | 148 | MagnesiumBy similarity | 1 | |
Binding sitei | 239 | SubstrateBy similarity | 1 | |
Binding sitei | 330 | ATPBy similarity | 1 | |
Metal bindingi | 391 | MagnesiumBy similarity | 1 | |
Binding sitei | 398 | ATPBy similarity | 1 | |
Binding sitei | 446 | ATPBy similarity | 1 | |
Binding sitei | 473 | SubstrateBy similarity | 1 | |
Binding sitei | 475 | ATPBy similarity | 1 | |
Binding sitei | 481 | ATP; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 387 – 396 | ATPBy similarity | 10 | |
Nucleotide bindingi | 420 – 423 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- glutathione binding Source: UniProtKB
- glutathione synthase activity Source: PomBase
- magnesium ion binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- cellular detoxification of cadmium ion Source: PomBase
- cellular response to cadmium ion Source: PomBase
- glutathione biosynthetic process Source: PomBase
- phytochelatin biosynthetic process Source: PomBase
- phytochelatin-metal complex formation Source: PomBase
Keywordsi
Molecular function | Ligase |
Biological process | Glutathione biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.3.2.3, 5613 |
Reactomei | R-SPO-174403, Glutathione synthesis and recycling |
UniPathwayi | UPA00142;UER00210 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione synthetase large chain (EC:6.3.2.3)Short name: GSH synthetase large chain Short name: GSH-S Alternative name(s): Glutathione synthase large chain Phytochelatin synthetase |
Gene namesi | Name:gsa1 Synonyms:gsh2 ORF Names:SPAC3F10.04 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC3F10.04, gsa1 |
VEuPathDBi | FungiDB:SPAC3F10.04 |
Subcellular locationi
Cytosol
- cytosol Source: PomBase
Other locations
- glutathione synthase complex Source: PomBase
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211265 | 1 – 498 | Glutathione synthetase large chainAdd BLAST | 498 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 356 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P35669 |
PaxDbi | P35669 |
PTM databases
iPTMneti | P35669 |
Interactioni
Subunit structurei
Heterodimer composed of a large and a small chain.
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 279340, 11 interactors |
STRINGi | 4896.SPAC3F10.04.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 150 – 153 | Substrate bindingBy similarity | 4 | |
Regioni | 233 – 235 | Substrate bindingBy similarity | 3 | |
Regioni | 291 – 294 | Substrate bindingBy similarity | 4 | |
Regioni | 484 – 485 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the eukaryotic GSH synthase family.Curated
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
HOGENOMi | CLU_025152_2_1_1 |
InParanoidi | P35669 |
OMAi | CPTVLTQ |
PhylomeDBi | P35669 |
Family and domain databases
CDDi | cd00228, eu-GS, 1 hit |
Gene3Di | 1.10.1080.10, 1 hit 3.30.1490.50, 1 hit 3.30.1490.80, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR014049, Glutathione_synthase_N_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 1 hit PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
i Sequence
Sequence statusi: Complete.
P35669-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEIEKYTPEQ IEELGKGARD FAFAHGVVFT ELSVSKEGRN IATQIPITLF
60 70 80 90 100
PSVIPHGAFV EAVSVQKAYN KLYAKIANDY EFLRLHLQSI TKYDEFMNKL
110 120 130 140 150
WNLYQKHREA VAHLKENQFQ PLSLGVFRSD YMVHQDDSFI GCKQVEFNTI
160 170 180 190 200
SVSFGGVSKA VSNLHAYCSQ SGLYRKPLTT NYLTVNTSVS GICTGISNAV
210 220 230 240 250
DAYRDYVKNI TSKMNIASDN TKPIVLFVVK GGERNITDQR TLEYELLNRF
260 270 280 290 300
HVISKRIDIA ELNSLIHDKS SNKLYMKTSF TTYEVAVVYY RVGYALDDYP
310 320 330 340 350
SQEAWDMRLT IENTLAIKCP SISTHLAGSK KIQQVLAESN ALERFLEGDE
360 370 380 390 400
LQAVRSTFAD MYPLDDTPRG KEGIKLAFEK PEDFVLKPQR EGGGNNTYGK
410 420 430 440 450
DIPGLLSKMP QEEWDSYILM RYINAVPSQN YILKGERPEK FDVVDEIGIL
460 470 480 490
GTIVWNIKTD EVVQNGQSGF ICRTKPKKTN EGGVATGYAS LSSIELSE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 132 | M → T in AAL41009 (Ref. 2) Curated | 1 | |
Sequence conflicti | 263 | N → T in AAA35307 (PubMed:1958212).Curated | 1 | |
Sequence conflicti | 304 | A → R in AAA35307 (PubMed:1958212).Curated | 1 | |
Sequence conflicti | 330 | K → E in AAA35307 (PubMed:1958212).Curated | 1 | |
Sequence conflicti | 458 | K → N in AAA35307 (PubMed:1958212).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAA93302.1 AF448236 Genomic DNA Translation: AAL41009.1 Y08414 Genomic DNA Translation: CAA69691.1 M85179 Genomic DNA Translation: AAA35307.1 |
PIRi | JT0961 T38705 |
RefSeqi | NP_593936.1, NM_001019364.2 |
Genome annotation databases
EnsemblFungii | SPAC3F10.04.1; SPAC3F10.04.1:pep; SPAC3F10.04 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAA93302.1 AF448236 Genomic DNA Translation: AAL41009.1 Y08414 Genomic DNA Translation: CAA69691.1 M85179 Genomic DNA Translation: AAA35307.1 |
PIRi | JT0961 T38705 |
RefSeqi | NP_593936.1, NM_001019364.2 |
3D structure databases
SMRi | P35669 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 279340, 11 interactors |
STRINGi | 4896.SPAC3F10.04.1 |
PTM databases
iPTMneti | P35669 |
Proteomic databases
MaxQBi | P35669 |
PaxDbi | P35669 |
Genome annotation databases
EnsemblFungii | SPAC3F10.04.1; SPAC3F10.04.1:pep; SPAC3F10.04 |
Organism-specific databases
PomBasei | SPAC3F10.04, gsa1 |
VEuPathDBi | FungiDB:SPAC3F10.04 |
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
HOGENOMi | CLU_025152_2_1_1 |
InParanoidi | P35669 |
OMAi | CPTVLTQ |
PhylomeDBi | P35669 |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00210 |
BRENDAi | 6.3.2.3, 5613 |
Reactomei | R-SPO-174403, Glutathione synthesis and recycling |
Miscellaneous databases
PROi | PR:P35669 |
Family and domain databases
CDDi | cd00228, eu-GS, 1 hit |
Gene3Di | 1.10.1080.10, 1 hit 3.30.1490.50, 1 hit 3.30.1490.80, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR014049, Glutathione_synthase_N_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 1 hit PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GSHB_SCHPO | |
Accessioni | P35669Primary (citable) accession number: P35669 Secondary accession number(s): Q8X1A1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families