UniProtKB - P35639 (DDIT3_MOUSE)
DNA damage-inducible transcript 3 protein
Ddit3
Functioni
GO - Molecular functioni
- cAMP response element binding protein binding Source: UniProtKB
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor inhibitor activity Source: ARUK-UCL
- leucine zipper domain binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: Ensembl
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- transcription factor binding Source: ParkinsonsUK-UCL
- transcription regulatory region sequence-specific DNA binding Source: ParkinsonsUK-UCL
GO - Biological processi
- apoptotic process Source: UniProtKB
- blood vessel maturation Source: UniProtKB
- cell cycle arrest Source: MGI
- cell redox homeostasis Source: MGI
- endoplasmic reticulum unfolded protein response Source: UniProtKB
- ER overload response Source: MGI
- establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to nitrosative stress Source: ParkinsonsUK-UCL
- negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- negative regulation of CREB transcription factor activity Source: MGI
- negative regulation of determination of dorsal identity Source: BHF-UCL
- negative regulation of DNA binding Source: ParkinsonsUK-UCL
- negative regulation of DNA-binding transcription factor activity Source: MGI
- negative regulation of fat cell differentiation Source: MGI
- negative regulation of interferon-gamma production Source: MGI
- negative regulation of interleukin-17 production Source: MGI
- negative regulation of interleukin-4 production Source: MGI
- negative regulation of myoblast differentiation Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: MGI
- negative regulation of protein kinase B signaling Source: MGI
- negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: ParkinsonsUK-UCL
- positive regulation of apoptotic process Source: MGI
- positive regulation of autophagy in response to ER overload Source: MGI
- positive regulation of DNA-binding transcription factor activity Source: ParkinsonsUK-UCL
- positive regulation of interleukin-8 production Source: MGI
- positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
- positive regulation of neuron apoptotic process Source: UniProtKB
- positive regulation of neuron death Source: ParkinsonsUK-UCL
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: ParkinsonsUK-UCL
- positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: MGI
- regulation of autophagy Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription involved in anterior/posterior axis specification Source: BHF-UCL
- release of sequestered calcium ion into cytosol Source: UniProtKB
- response to endoplasmic reticulum stress Source: UniProtKB
- response to starvation Source: UniProtKB
- response to unfolded protein Source: MGI
- sensory perception of sound Source: MGI
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Apoptosis, Cell cycle, Growth arrest, Stress response, Transcription, Transcription regulation, Unfolded protein response, Wnt signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: DNA damage-inducible transcript 3 proteinShort name: DDIT-3 Alternative name(s): C/EBP zeta C/EBP-homologous protein1 Publication Short name: CHOP1 Publication C/EBP-homologous protein 10 Short name: CHOP-10 CCAAT/enhancer-binding protein homologous protein Growth arrest and DNA-damage-inducible protein GADD153 |
Gene namesi | Name:Ddit3 Synonyms:Chop1 Publication, Chop10, Gadd153 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109247, Ddit3 |
Subcellular locationi
Endosome
- late endosome Source: MGI
Nucleus
- nucleus Source: UniProtKB
Other locations
- CHOP-ATF3 complex Source: MGI
- CHOP-ATF4 complex Source: MGI
- CHOP-C/EBP complex Source: ParkinsonsUK-UCL
- cytoplasm Source: ParkinsonsUK-UCL
- protein-DNA complex Source: MGI
- RNA polymerase II transcription regulator complex Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | S → A: Loss of phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 15 | S → A: Loss of phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 30 | S → A: Loss of phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 31 | S → A: Loss of phosphorylation. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2146304 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076643 | 1 – 168 | DNA damage-inducible transcript 3 proteinAdd BLAST | 168 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 15 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 30 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 31 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 78 | Phosphoserine; by MAPK141 Publication | 1 | |
Modified residuei | 81 | Phosphoserine; by MAPK141 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P35639 |
PaxDbi | P35639 |
PRIDEi | P35639 |
PTM databases
iPTMneti | P35639 |
PhosphoSitePlusi | P35639 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000025408, Expressed in islet of Langerhans and 303 other tissues |
Genevisiblei | P35639, MM |
Interactioni
Subunit structurei
Heterodimer.
Interacts with TCF7L2/TCF4, EP300/P300, HDAC5 and HDAC6 (By similarity).
Interacts with TRIB3 which blocks its association with EP300/P300 (By similarity).
Interacts with FOXO3, and CEBPB (By similarity).
Interacts with ATF4 (PubMed:23624402).
Interacts with HDAC1 (PubMed:22242125).
By similarity2 PublicationsBinary interactionsi
P35639
With | #Exp. | IntAct |
---|---|---|
Atf4 [Q06507] | 3 | EBI-10636142,EBI-77383 |
GO - Molecular functioni
- cAMP response element binding protein binding Source: UniProtKB
- leucine zipper domain binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: Ensembl
- transcription factor binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 199078, 4 interactors |
ComplexPortali | CPX-65, CHOP-C/EBPalpha complex CPX-66, CHOP-C/EBPbeta complex |
DIPi | DIP-60705N |
IntActi | P35639, 1 interactor |
STRINGi | 10090.ENSMUSP00000026475 |
Chemistry databases
BindingDBi | P35639 |
Miscellaneous databases
RNActi | P35639, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 98 – 161 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 10 – 26 | N-terminalBy similarityAdd BLAST | 17 | |
Regioni | 10 – 18 | Interaction with TRIB3By similarity | 9 | |
Regioni | 100 – 129 | Basic motifPROSITE-ProRule annotationAdd BLAST | 30 | |
Regioni | 133 – 147 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 92 – 97 | Poly-Glu | 6 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3119, Eukaryota |
GeneTreei | ENSGT00390000006305 |
InParanoidi | P35639 |
OMAi | QCPARAG |
OrthoDBi | 1338770at2759 |
PhylomeDBi | P35639 |
TreeFami | TF105006 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR016670, DNA_damage_induc_transcript_3 |
PANTHERi | PTHR16833, PTHR16833, 2 hits |
PIRSFi | PIRSF016571, C/EBPzeta_CHOP_DDIT3, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAESLPFTL ETVSSWELEA WYEDLQEVLS SDEIGGTYIS SPGNEEEESK
60 70 80 90 100
TFTTLDPASL AWLTEEPGPT EVTRTSQSPR SPDSSQSSMA QEEEEEEQGR
110 120 130 140 150
TRKRKQSGQC PARPGKQRMK EKEQENERKV AQLAEENERL KQEIERLTRE
160
VETTRRALID RMVSLHQA
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YX14 | D3YX14_MOUSE | C/EBP zeta | Ddit3 | 160 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 34 | I → N in AAH13718 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67083 mRNA Translation: CAA47465.1 BC013718 mRNA Translation: AAH13718.1 |
CCDSi | CCDS24236.1 [P35639-1] |
PIRi | S26148 |
RefSeqi | NP_001277112.1, NM_001290183.1 [P35639-1] NP_031863.3, NM_007837.4 [P35639-1] XP_006513260.1, XM_006513197.3 [P35639-1] |
Genome annotation databases
Ensembli | ENSMUST00000026475; ENSMUSP00000026475; ENSMUSG00000025408 [P35639-1] |
GeneIDi | 13198 |
KEGGi | mmu:13198 |
UCSCi | uc007hiy.2, mouse [P35639-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67083 mRNA Translation: CAA47465.1 BC013718 mRNA Translation: AAH13718.1 |
CCDSi | CCDS24236.1 [P35639-1] |
PIRi | S26148 |
RefSeqi | NP_001277112.1, NM_001290183.1 [P35639-1] NP_031863.3, NM_007837.4 [P35639-1] XP_006513260.1, XM_006513197.3 [P35639-1] |
3D structure databases
SMRi | P35639 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199078, 4 interactors |
ComplexPortali | CPX-65, CHOP-C/EBPalpha complex CPX-66, CHOP-C/EBPbeta complex |
DIPi | DIP-60705N |
IntActi | P35639, 1 interactor |
STRINGi | 10090.ENSMUSP00000026475 |
Chemistry databases
BindingDBi | P35639 |
ChEMBLi | CHEMBL2146304 |
PTM databases
iPTMneti | P35639 |
PhosphoSitePlusi | P35639 |
Proteomic databases
MaxQBi | P35639 |
PaxDbi | P35639 |
PRIDEi | P35639 |
Protocols and materials databases
Antibodypediai | 4408, 956 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026475; ENSMUSP00000026475; ENSMUSG00000025408 [P35639-1] |
GeneIDi | 13198 |
KEGGi | mmu:13198 |
UCSCi | uc007hiy.2, mouse [P35639-1] |
Organism-specific databases
CTDi | 1649 |
MGIi | MGI:109247, Ddit3 |
Phylogenomic databases
eggNOGi | KOG3119, Eukaryota |
GeneTreei | ENSGT00390000006305 |
InParanoidi | P35639 |
OMAi | QCPARAG |
OrthoDBi | 1338770at2759 |
PhylomeDBi | P35639 |
TreeFami | TF105006 |
Miscellaneous databases
BioGRID-ORCSi | 13198, 1 hit in 18 CRISPR screens |
ChiTaRSi | Ddit3, mouse |
PROi | PR:P35639 |
RNActi | P35639, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025408, Expressed in islet of Langerhans and 303 other tissues |
Genevisiblei | P35639, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR016670, DNA_damage_induc_transcript_3 |
PANTHERi | PTHR16833, PTHR16833, 2 hits |
PIRSFi | PIRSF016571, C/EBPzeta_CHOP_DDIT3, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDIT3_MOUSE | |
Accessioni | P35639Primary (citable) accession number: P35639 Secondary accession number(s): Q91YW9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families