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Protein

Beta-adrenergic receptor kinase 2

Gene

GRK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Catalytic activityi

ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1
Active sitei317Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.15 2681
ReactomeiR-HSA-418555 G alpha (s) signalling events
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
SignaLinkiP35626
SIGNORiP35626

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-adrenergic receptor kinase 2 (EC:2.7.11.15)
Short name:
Beta-ARK-2
Alternative name(s):
G-protein-coupled receptor kinase 3Imported
Gene namesi
Name:GRK3Imported
Synonyms:ADRBK2, BARK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100077.14
HGNCiHGNC:290 GRK3
MIMi109636 gene
neXtProtiNX_P35626

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi157
OpenTargetsiENSG00000100077
PharmGKBiPA41

Chemistry databases

ChEMBLiCHEMBL1075166
DrugBankiDB00171 Adenosine triphosphate
GuidetoPHARMACOLOGYi1467

Polymorphism and mutation databases

BioMutaiADRBK2
DMDMi116241253

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856321 – 688Beta-adrenergic receptor kinase 2Add BLAST688

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiP35626
MaxQBiP35626
PaxDbiP35626
PeptideAtlasiP35626
PRIDEiP35626
ProteomicsDBi55118

PTM databases

GlyConnecti1034
iPTMnetiP35626
PhosphoSitePlusiP35626

Expressioni

Gene expression databases

BgeeiENSG00000100077 Expressed in 200 organ(s), highest expression level in Brodmann (1909) area 23
CleanExiHS_ADRBK2
ExpressionAtlasiP35626 baseline and differential
GenevisibleiP35626 HS

Organism-specific databases

HPAiHPA000804

Interactioni

Protein-protein interaction databases

BioGridi106666, 10 interactors
IntActiP35626, 4 interactors
STRINGi9606.ENSP00000317578

Chemistry databases

BindingDBiP35626

Structurei

3D structure databases

ProteinModelPortaliP35626
SMRiP35626
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 175RGSPROSITE-ProRule annotationAdd BLAST122
Domaini191 – 453Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini454 – 521AGC-kinase C-terminalAdd BLAST68
Domaini558 – 652PHPROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190N-terminalAdd BLAST190

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
HOGENOMiHOG000006742
HOVERGENiHBG050559
InParanoidiP35626
KOiK00910
OMAiDHVQTHL
OrthoDBiEOG091G0AEI
PhylomeDBiP35626
TreeFamiTF313940

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P35626-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADLEAVLAD VSYLMAMEKS KATPAARASK RIVLPEPSIR SVMQKYLAER
60 70 80 90 100
NEITFDKIFN QKIGFLLFKD FCLNEINEAV PQVKFYEEIK EYEKLDNEED
110 120 130 140 150
RLCRSRQIYD AYIMKELLSC SHPFSKQAVE HVQSHLSKKQ VTSTLFQPYI
160 170 180 190 200
EEICESLRGD IFQKFMESDK FTRFCQWKNV ELNIHLTMNE FSVHRIIGRG
210 220 230 240 250
GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD
260 270 280 290 300
CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE
310 320 330 340 350
IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS
360 370 380 390 400
VGTHGYMAPE VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH
410 420 430 440 450
EIDRMTLTVN VELPDTFSPE LKSLLEGLLQ RDVSKRLGCH GGGSQEVKEH
460 470 480 490 500
SFFKGVDWQH VYLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD
510 520 530 540 550
CDQELYKNFP LVISERWQQE VTETVYEAVN ADTDKIEARK RAKNKQLGHE
560 570 580 590 600
EDYALGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL
610 620 630 640 650
LTMEQILSVE ETQIKDKKCI LFRIKGGKQF VLQCESDPEF VQWKKELNET
660 670 680
FKEAQRLLRR APKFLNKPRS GTVELPKPSL CHRNSNGL
Length:688
Mass (Da):79,710
Last modified:October 17, 2006 - v2
Checksum:i50844236A01C1423
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8N433Q8N433_HUMAN
G protein-coupled receptor kinase
GRK3
387Annotation score:
H7C099H7C099_HUMAN
Beta-adrenergic receptor kinase 2
GRK3
45Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti308M → V in CAA48870 (PubMed:8427589).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04038050R → S1 PublicationCorresponds to variant dbSNP:rs55700971Ensembl.1
Natural variantiVAR_04038160N → S1 PublicationCorresponds to variant dbSNP:rs55740593Ensembl.1
Natural variantiVAR_040382104R → K in a lung bronchoalveolar carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_028005409V → M. Corresponds to variant dbSNP:rs2272859Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69117 mRNA Translation: CAA48870.1
AL022329 Genomic DNA No translation available.
CCDSiCCDS13832.1
PIRiJC1469
RefSeqiNP_005151.2, NM_005160.3
UniGeneiHs.657494
Hs.733960

Genome annotation databases

EnsembliENST00000324198; ENSP00000317578; ENSG00000100077
GeneIDi157
KEGGihsa:157
UCSCiuc003abx.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69117 mRNA Translation: CAA48870.1
AL022329 Genomic DNA No translation available.
CCDSiCCDS13832.1
PIRiJC1469
RefSeqiNP_005151.2, NM_005160.3
UniGeneiHs.657494
Hs.733960

3D structure databases

ProteinModelPortaliP35626
SMRiP35626
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106666, 10 interactors
IntActiP35626, 4 interactors
STRINGi9606.ENSP00000317578

Chemistry databases

BindingDBiP35626
ChEMBLiCHEMBL1075166
DrugBankiDB00171 Adenosine triphosphate
GuidetoPHARMACOLOGYi1467

PTM databases

GlyConnecti1034
iPTMnetiP35626
PhosphoSitePlusiP35626

Polymorphism and mutation databases

BioMutaiADRBK2
DMDMi116241253

Proteomic databases

EPDiP35626
MaxQBiP35626
PaxDbiP35626
PeptideAtlasiP35626
PRIDEiP35626
ProteomicsDBi55118

Protocols and materials databases

DNASUi157
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324198; ENSP00000317578; ENSG00000100077
GeneIDi157
KEGGihsa:157
UCSCiuc003abx.5 human

Organism-specific databases

CTDi157
DisGeNETi157
EuPathDBiHostDB:ENSG00000100077.14
GeneCardsiGRK3
H-InvDBiHIX0041309
HGNCiHGNC:290 GRK3
HPAiHPA000804
MIMi109636 gene
neXtProtiNX_P35626
OpenTargetsiENSG00000100077
PharmGKBiPA41
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
HOGENOMiHOG000006742
HOVERGENiHBG050559
InParanoidiP35626
KOiK00910
OMAiDHVQTHL
OrthoDBiEOG091G0AEI
PhylomeDBiP35626
TreeFamiTF313940

Enzyme and pathway databases

BRENDAi2.7.11.15 2681
ReactomeiR-HSA-418555 G alpha (s) signalling events
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
SignaLinkiP35626
SIGNORiP35626

Miscellaneous databases

ChiTaRSiGRK3 human
GeneWikiiBeta_adrenergic_receptor_kinase-2
GenomeRNAii157
PROiPR:P35626
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100077 Expressed in 200 organ(s), highest expression level in Brodmann (1909) area 23
CleanExiHS_ADRBK2
ExpressionAtlasiP35626 baseline and differential
GenevisibleiP35626 HS

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARBK2_HUMAN
AccessioniPrimary (citable) accession number: P35626
Secondary accession number(s): Q9UGW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 17, 2006
Last modified: November 7, 2018
This is version 183 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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