UniProtKB - P35611 (ADDA_HUMAN)
Protein
Alpha-adducin
Gene
ADD1
Organism
Homo sapiens (Human)
Status
Functioni
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
GO - Molecular functioni
- actin binding Source: ProtInc
- actin filament binding Source: BHF-UCL
- cadherin binding Source: BHF-UCL
- calmodulin binding Source: UniProtKB-KW
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- RNA binding Source: UniProtKB
- spectrin binding Source: BHF-UCL
- structural constituent of cytoskeleton Source: Ensembl
- structural molecule activity Source: GO_Central
- transcription factor binding Source: BHF-UCL
GO - Biological processi
- actin cytoskeleton organization Source: BHF-UCL
- actin filament bundle assembly Source: BHF-UCL
- barbed-end actin filament capping Source: BHF-UCL
- cell morphogenesis Source: Ensembl
- cellular response to calcium ion Source: CAFA
- cell volume homeostasis Source: Ensembl
- erythrocyte differentiation Source: Ensembl
- hemoglobin metabolic process Source: Ensembl
- homeostasis of number of cells within a tissue Source: Ensembl
- in utero embryonic development Source: Ensembl
- IRE1-mediated unfolded protein response Source: Reactome
- multicellular organism growth Source: Ensembl
- positive regulation of adherens junction organization Source: CAFA
- positive regulation of establishment of endothelial barrier Source: CAFA
- positive regulation of protein binding Source: BHF-UCL
- transmembrane transport Source: Reactome
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding |
Enzyme and pathway databases
PathwayCommonsi | P35611 |
Reactomei | R-HSA-264870, Caspase-mediated cleavage of cytoskeletal proteins R-HSA-381038, XBP1(S) activates chaperone genes R-HSA-5223345, Miscellaneous transport and binding events |
SIGNORi | P35611 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-adducinAlternative name(s): Erythrocyte adducin subunit alpha |
Gene namesi | Name:ADD1 Synonyms:ADDA |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000087274.16 |
HGNCi | HGNC:243, ADD1 |
MIMi | 102680, gene |
neXtProti | NX_P35611 |
Subcellular locationi
Plasma membrane
Cytoskeleton
Cytoskeleton
- cytoskeleton Source: GO_Central
- F-actin capping protein complex Source: BHF-UCL
Cytosol
- cytosol Source: Reactome
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: BHF-UCL
Plasma Membrane
- plasma membrane Source: HPA
- plasma membrane raft Source: GO_Central
Other locations
- adherens junction Source: CAFA
- cytoplasm Source: CAFA
- focal adhesion Source: UniProtKB
- postsynaptic density Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 445 | T → D: Abolishes phosphorylation by ROCK2; when associated with D-480. 1 Publication | 1 | |
Mutagenesisi | 480 | T → D: Abolishes phosphorylation by ROCK2; when associated with D-445. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 118 |
MalaCardsi | ADD1 |
OpenTargetsi | ENSG00000087274 |
PharmGKBi | PA31 |
Miscellaneous databases
Pharosi | P35611, Tbio |
Polymorphism and mutation databases
BioMutai | ADD1 |
DMDMi | 12644231 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218530 | 1 – 737 | Alpha-adducinAdd BLAST | 737 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 59 | Phosphoserine; by PKACombined sources1 Publication | 1 | |
Modified residuei | 64 | PhosphoserineCombined sources | 1 | |
Modified residuei | 331 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 334 | PhosphoserineCombined sources | 1 | |
Modified residuei | 353 | PhosphoserineCombined sources | 1 | |
Modified residuei | 355 | PhosphoserineCombined sources | 1 | |
Modified residuei | 358 | PhosphoserineCombined sources | 1 | |
Modified residuei | 366 | PhosphoserineCombined sources | 1 | |
Modified residuei | 408 | Phosphoserine; by PKA1 Publication | 1 | |
Modified residuei | 427 | PhosphoserineBy similarity | 1 | |
Modified residuei | 429 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 431 | PhosphoserineCombined sources | 1 | |
Modified residuei | 436 | Phosphoserine; by PKACombined sources1 Publication | 1 | |
Modified residuei | 445 | Phosphothreonine; by ROCK21 Publication | 1 | |
Modified residuei | 464 | PhosphoserineBy similarity | 1 | |
Modified residuei | 465 | PhosphoserineCombined sources | 1 | |
Modified residuei | 480 | Phosphothreonine; by ROCK21 Publication | 1 | |
Modified residuei | 481 | Phosphoserine; by PKA1 Publication | 1 | |
Modified residuei | 586 | PhosphoserineBy similarity | 1 | |
Modified residuei | 600 | PhosphoserineBy similarity | 1 | |
Modified residuei | 613 | PhosphoserineBy similarity | 1 | |
Modified residuei | 614 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 678 | PhosphoserineCombined sources | 1 | |
Modified residuei | 707 | PhosphoserineCombined sources | 1 | |
Modified residuei | 710 | PhosphoserineCombined sources | 1 | |
Modified residuei | 714 | PhosphoserineCombined sources | 1 | |
Modified residuei | 716 | Phosphoserine; by PKCCombined sources1 Publication | 1 | |
Modified residuei | 726 | Phosphoserine; by PKA and PKC1 Publication | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P35611 |
jPOSTi | P35611 |
MassIVEi | P35611 |
MaxQBi | P35611 |
PaxDbi | P35611 |
PeptideAtlasi | P35611 |
PRIDEi | P35611 |
ProteomicsDBi | 289 55102 [P35611-1] 55103 [P35611-2] 55104 [P35611-3] 70300 |
PTM databases
GlyGeni | P35611, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P35611 |
MetOSitei | P35611 |
PhosphoSitePlusi | P35611 |
SwissPalmi | P35611 |
Expressioni
Tissue specificityi
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Gene expression databases
Bgeei | ENSG00000087274, Expressed in primary visual cortex and 248 other tissues |
ExpressionAtlasi | P35611, baseline and differential |
Genevisiblei | P35611, HS |
Organism-specific databases
HPAi | ENSG00000087274, Low tissue specificity |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.
Binary interactionsi
Isoform 5 [P35611-5]
With | #Exp. | IntAct |
---|---|---|
HMG20A [Q9NP66] | 3 | EBI-10206868,EBI-740641 |
GO - Molecular functioni
- actin binding Source: ProtInc
- actin filament binding Source: BHF-UCL
- cadherin binding Source: BHF-UCL
- calmodulin binding Source: UniProtKB-KW
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- spectrin binding Source: BHF-UCL
- transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 106631, 76 interactors |
IntActi | P35611, 50 interactors |
MINTi | P35611 |
STRINGi | 9606.ENSP00000264758 |
Miscellaneous databases
RNActi | P35611, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 717 – 734 | Interaction with calmodulinSequence analysisAdd BLAST | 18 |
Domaini
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3699, Eukaryota |
GeneTreei | ENSGT00940000158581 |
InParanoidi | P35611 |
OMAi | FMLHSAI |
OrthoDBi | 400524at2759 |
PhylomeDBi | P35611 |
TreeFami | TF313003 |
Family and domain databases
DisProti | DP00240 |
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027766, ADD1 IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF4, PTHR10672:SF4, 1 hit |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 6 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P35611-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNGDSRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN
60 70 80 90 100
MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTT
110 120 130 140 150
NVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA
160 170 180 190 200
AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKI
210 220 230 240 250
NLQGDIVDRG STNLGVNQAG FTLHSAIYAA RPDVKCVVHI HTPAGAAVSA
260 270 280 290 300
MKCGLLPISP EALSLGEVAY HDYHGILVDE EEKVLIQKNL GPKSKVLILR
310 320 330 340 350
NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLNPEKYK
360 370 380 390 400
AKSRSPGSPV GEGTGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL
410 420 430 440 450
REKSKKYSDV EVPASVTGYS FASDGDSGTC SPLRHSFQKQ QREKTRWLNS
460 470 480 490 500
GRGDEASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ
510 520 530 540 550
EMRNKIREQN LQDIKTAGPQ SQVLCGVVMD RSLVQGELVT ASKAIIEKEY
560 570 580 590 600
QPHVIVSTTG PNPFTTLTDR ELEEYRREVE RKQKGSEENL DEAREQKEKS
610 620 630 640 650
PPDQPAVPHP PPSTPIKLEE DLVPEPTTGD DSDAATFKPT LPDLSPDEPS
660 670 680 690 700
EALGFPMLEK EEEAHRPPSP TEAPTEASPE PAPDPAPVAE EAAPSAVEEG
710 720 730
AAADPGSDGS PGKSPSKKKK KFRTPSFLKK SKKKSDS
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y9H2 | H0Y9H2_HUMAN | Alpha-adducin | ADD1 | 444 | Annotation score: | ||
E7ENY0 | E7ENY0_HUMAN | Alpha-adducin | ADD1 | 663 | Annotation score: | ||
E7EV99 | E7EV99_HUMAN | Alpha-adducin | ADD1 | 632 | Annotation score: | ||
D6RF25 | D6RF25_HUMAN | Alpha-adducin | ADD1 | 142 | Annotation score: | ||
A0A0A0MSR2 | A0A0A0MSR2_HUMAN | Alpha-adducin | ADD1 | 400 | Annotation score: | ||
H0YG19 | H0YG19_HUMAN | Alpha-adducin | ADD1 | 114 | Annotation score: | ||
D6RAH3 | D6RAH3_HUMAN | Alpha-adducin | ADD1 | 137 | Annotation score: | ||
D6RJE2 | D6RJE2_HUMAN | Alpha-adducin | ADD1 | 69 | Annotation score: | ||
H0YFD8 | H0YFD8_HUMAN | Alpha-adducin | ADD1 | 105 | Annotation score: |
Sequence cautioni
The sequence CAA98970 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 606 | A → E in AAB05645 (PubMed:7774961).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_022108 | 6 | R → C. Corresponds to variant dbSNP:rs2295497Ensembl. | 1 | |
Natural variantiVAR_014863 | 270 | Y → N. Corresponds to variant dbSNP:rs4971Ensembl. | 1 | |
Natural variantiVAR_014864 | 376 | E → D. Corresponds to variant dbSNP:rs4972Ensembl. | 1 | |
Natural variantiVAR_014184 | 460 | G → W2 PublicationsCorresponds to variant dbSNP:rs4961EnsemblClinVar. | 1 | |
Natural variantiVAR_014865 | 510 | N → I. Corresponds to variant dbSNP:rs4962Ensembl. | 1 | |
Natural variantiVAR_014185 | 586 | S → C2 PublicationsCorresponds to variant dbSNP:rs4963Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000174 | 471 | K → KVWTNITHDHVKPLLQSLSS GVCVPSCITNCL in isoform 3 and isoform 6. 1 Publication | 1 | |
Alternative sequenceiVSP_055402 | 472 – 511 | WTKED…REQNL → VWTNITHDHVKPLLQSLSSG VCVPSCITNCLVCAYLTVHS in isoform 5. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_055403 | 512 – 737 | Missing in isoform 5. 1 PublicationAdd BLAST | 226 | |
Alternative sequenceiVSP_054420 | 535 | Q → QDAPLSDCTETIEGLELTEQ TFSPAKSLSFRK in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_000175 | 621 – 631 | DLVPEPTTGDD → GDGCAREYLLP in isoform 2, isoform 4 and isoform 6. 2 PublicationsAdd BLAST | 11 | |
Alternative sequenceiVSP_000176 | 632 – 737 | Missing in isoform 2, isoform 4 and isoform 6. 2 PublicationsAdd BLAST | 106 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000264758; ENSP00000264758; ENSG00000087274 [P35611-3] ENST00000355842; ENSP00000348100; ENSG00000087274 [P35611-4] ENST00000398123; ENSP00000381191; ENSG00000087274 [P35611-6] ENST00000398125; ENSP00000381193; ENSG00000087274 [P35611-6] ENST00000398129; ENSP00000381197; ENSG00000087274 [P35611-1] ENST00000651918; ENSP00000498269; ENSG00000087274 [P35611-2] |
GeneIDi | 118 |
KEGGi | hsa:118 |
UCSCi | uc003gfo.4, human [P35611-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | P35611 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 106631, 76 interactors |
IntActi | P35611, 50 interactors |
MINTi | P35611 |
STRINGi | 9606.ENSP00000264758 |
PTM databases
GlyGeni | P35611, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P35611 |
MetOSitei | P35611 |
PhosphoSitePlusi | P35611 |
SwissPalmi | P35611 |
Polymorphism and mutation databases
BioMutai | ADD1 |
DMDMi | 12644231 |
Proteomic databases
EPDi | P35611 |
jPOSTi | P35611 |
MassIVEi | P35611 |
MaxQBi | P35611 |
PaxDbi | P35611 |
PeptideAtlasi | P35611 |
PRIDEi | P35611 |
ProteomicsDBi | 289 55102 [P35611-1] 55103 [P35611-2] 55104 [P35611-3] 70300 |
Protocols and materials databases
Antibodypediai | 4065, 739 antibodies |
DNASUi | 118 |
Genome annotation databases
Ensembli | ENST00000264758; ENSP00000264758; ENSG00000087274 [P35611-3] ENST00000355842; ENSP00000348100; ENSG00000087274 [P35611-4] ENST00000398123; ENSP00000381191; ENSG00000087274 [P35611-6] ENST00000398125; ENSP00000381193; ENSG00000087274 [P35611-6] ENST00000398129; ENSP00000381197; ENSG00000087274 [P35611-1] ENST00000651918; ENSP00000498269; ENSG00000087274 [P35611-2] |
GeneIDi | 118 |
KEGGi | hsa:118 |
UCSCi | uc003gfo.4, human [P35611-1] |
Organism-specific databases
CTDi | 118 |
DisGeNETi | 118 |
EuPathDBi | HostDB:ENSG00000087274.16 |
GeneCardsi | ADD1 |
HGNCi | HGNC:243, ADD1 |
HPAi | ENSG00000087274, Low tissue specificity |
MalaCardsi | ADD1 |
MIMi | 102680, gene |
neXtProti | NX_P35611 |
OpenTargetsi | ENSG00000087274 |
PharmGKBi | PA31 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3699, Eukaryota |
GeneTreei | ENSGT00940000158581 |
InParanoidi | P35611 |
OMAi | FMLHSAI |
OrthoDBi | 400524at2759 |
PhylomeDBi | P35611 |
TreeFami | TF313003 |
Enzyme and pathway databases
PathwayCommonsi | P35611 |
Reactomei | R-HSA-264870, Caspase-mediated cleavage of cytoskeletal proteins R-HSA-381038, XBP1(S) activates chaperone genes R-HSA-5223345, Miscellaneous transport and binding events |
SIGNORi | P35611 |
Miscellaneous databases
BioGRID-ORCSi | 118, 6 hits in 845 CRISPR screens |
ChiTaRSi | ADD1, human |
GeneWikii | ADD1 |
GenomeRNAii | 118 |
Pharosi | P35611, Tbio |
PROi | PR:P35611 |
RNActi | P35611, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000087274, Expressed in primary visual cortex and 248 other tissues |
ExpressionAtlasi | P35611, baseline and differential |
Genevisiblei | P35611, HS |
Family and domain databases
DisProti | DP00240 |
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027766, ADD1 IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF4, PTHR10672:SF4, 1 hit |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADDA_HUMAN | |
Accessioni | P35611Primary (citable) accession number: P35611 Secondary accession number(s): A2A3N8 Q9UJB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | December 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 203 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations