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Protein

Replication factor C subunit 1

Gene

Rfc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi635 – 642ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDNA replication, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 1
Alternative name(s):
A1-P145
Activator 1 140 kDa subunit
Short name:
A1 140 kDa subunit
Activator 1 large subunit
Activator 1 subunit 1
Differentiation-specific element-binding protein
ISRE-binding protein
Replication factor C 140 kDa subunit
Short name:
RF-C 140 kDa subunit
Short name:
RFC140
Replication factor C large subunit
Gene namesi
Name:Rfc1
Synonyms:Ibf-1, Recc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97891 Rfc1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217731 – 1131Replication factor C subunit 1Add BLAST1131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei66PhosphotyrosineBy similarity1
Modified residuei68PhosphoserineCombined sources1
Modified residuei70PhosphoserineCombined sources1
Modified residuei72PhosphoserineBy similarity1
Modified residuei107PhosphoserineCombined sources1
Modified residuei109PhosphothreonineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei160PhosphothreonineCombined sources1
Modified residuei162PhosphothreonineBy similarity1
Modified residuei163PhosphoserineBy similarity1
Modified residuei172PhosphoserineBy similarity1
Modified residuei189PhosphoserineBy similarity1
Modified residuei244PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei253PhosphoserineCombined sources1
Modified residuei281PhosphoserineBy similarity1
Modified residuei309PhosphoserineBy similarity1
Modified residuei365PhosphoserineBy similarity1
Modified residuei535PhosphoserineCombined sources1
Modified residuei1090PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP35601
PaxDbiP35601
PeptideAtlasiP35601
PRIDEiP35601

PTM databases

iPTMnetiP35601
PhosphoSitePlusiP35601
SwissPalmiP35601

Expressioni

Gene expression databases

CleanExiMM_RFC1

Interactioni

Subunit structurei

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202846, 5 interactors
ComplexPortaliCPX-472 DNA replication factor C complex
CORUMiP35601
STRINGi10090.ENSMUSP00000031092

Structurei

3D structure databases

ProteinModelPortaliP35601
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini399 – 489BRCTPROSITE-ProRule annotationAdd BLAST91

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni354 – 528Interferon-stimulated-response-element binding regionAdd BLAST175

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1104 – 1108Nuclear localization signalSequence analysis5

Sequence similaritiesi

Belongs to the activator 1 large subunit family.Curated

Phylogenomic databases

eggNOGiENOG410IPR8 Eukaryota
COG5275 LUCA
HOVERGENiHBG004167
InParanoidiP35601
KOiK10754
PhylomeDBiP35601

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR013725 DNA_replication_fac_RFC1_C
IPR027417 P-loop_NTPase
IPR012178 RFC1
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF00533 BRCT, 1 hit
PF08519 RFC1, 1 hit
PIRSFiPIRSF036578 RFC1, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00292 BRCT, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52113 SSF52113, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P35601-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDIRKFFGVI SSGKKPVNET VKNEKTKASE GTVKGKKGVK EAKVNNSGKE
60 70 80 90 100
DASKPKQHSK KKRIIYDSDS ESEETVQVKN AKKKSEKLSL SYKPGKVSQK
110 120 130 140 150
DPVTYVSETD EDDDFVCKKA ASKSKENGVS TNSYLGTSNV KKNEENVKTK
160 170 180 190 200
NKPLSPIKLT PTSVLDYFGT ESVQRSGKKM VTSKRKESSQ NTEDSRLNDE
210 220 230 240 250
AIAKQLQLDE DAELERQLHE DEEFARTLAL LDEEPKIKKA RKDSEEGEES
260 270 280 290 300
FSSVQDDLSK AEKQKSPNKA ELFSTARKTY SPAKHGKGRA SEDAKQPCKS
310 320 330 340 350
AHRKEACSSP KASAKLALMK AKEESSYNET ELLAARRKES ATEPKGEKTT
360 370 380 390 400
PKKTKVSPTK RESVSPEDSE KKRTNYQAYR SYLNREGPKA LGSKEIPKGA
410 420 430 440 450
ENCLEGLTFV ITGVLESIER DEAKSLIERY GGKVTGNVSK KTNYLVMGRD
460 470 480 490 500
SGQSKSDKAA ALGTKILDED GLLDLIRTMP GKRSKYEMAA EAEMKKEKSK
510 520 530 540 550
LERTPQKNDQ GKRKISPAKK ESESKKCKLT LLKNSPMKAV KKEASTCPRG
560 570 580 590 600
LDVKETHGNR SSNKEECLLW VDKYKPASLK NIIGQQGDQS CANKLLRWLR
610 620 630 640 650
NWHKSSPEEK KHAAKFGKLA SKDDGSSFKA ALLSGPPGVG KTTTASLVCQ
660 670 680 690 700
ELGYSYVELN ASDTRSKNSL KAVVAESLNN TSIKGFYTSG AAPSVSARHA
710 720 730 740 750
LIMDEVDGMA GNEDRGGIQE LIGLIKHTKI PIICMCNDRN HPKIRSLVHY
760 770 780 790 800
CFDLRFQRPR VEQIKSAMLS IAFKEGLKIP PPAMNEIILG ANQDVRQVLH
810 820 830 840 850
NLSMWCAQSK ALTYDQAKAD SQRAKKDIRL GPFDVTRKVF AAGEETAHMS
860 870 880 890 900
LMDKSDLFFH DYSIAPLFVQ ENYLHVKPVA AGGDMKKHLM LLSRAADSIC
910 920 930 940 950
DGDLVDNQIR SKQNWSLLPT QAIYASVLPG ELMRGYMTQF PSFPSWLGKH
960 970 980 990 1000
SSTGKHDRIV QDLSLHMSLR TYSSKRTVNM DYLSHIRDAL VRPLTSQGVE
1010 1020 1030 1040 1050
GAQHVIKLMD TYYLMKEDFE NIMEVSSWGG KPSAFSKLDP KVKAAFTRAY
1060 1070 1080 1090 1100
NKEAHLTPYS LQVVKTSRLS TGPALDSEYS EEFQEDDTQS EKEQDAVETD
1110 1120 1130
AMIKKKTRSS KPSKSEREKE SKKGKGKNWK K
Length:1,131
Mass (Da):125,985
Last modified:October 1, 1996 - v2
Checksum:iA6F4F970A7F9EE94
GO
Isoform 2 (identifier: P35601-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     614-614: Missing.

Note: Alternative use of an acceptor site.
Show »
Length:1,130
Mass (Da):125,914
Checksum:i4EF6B8B2B58F1EDA
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5U4B1Q5U4B1_MOUSE
Replication factor C subunit 1
Rfc1
1,132Annotation score:
G3X8Z6G3X8Z6_MOUSE
Replication factor C subunit 1
Rfc1 Recc1, mCG_10254
1,131Annotation score:
A0A0N5E9G7A0A0N5E9G7_MOUSE
Replication factor C subunit 1
Rfc1
1,130Annotation score:
G3UWX1G3UWX1_MOUSE
Replication factor C subunit 1
Rfc1
1,145Annotation score:
A0A0N4SVN6A0A0N4SVN6_MOUSE
Replication factor C subunit 1
Rfc1
212Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66Y → N in AAA79698 (PubMed:7659092).Curated1
Sequence conflicti187E → EPDFCLSCLIFFGIQ in AAC52140 (Ref. 4) Curated1
Sequence conflicti254V → A in AAB60452 (Ref. 5) Curated1
Sequence conflicti559N → S in AAC52140 (Ref. 4) Curated1
Sequence conflicti945S → N in AAA21643 (PubMed:8265586).Curated1
Sequence conflicti1071T → A in AAA79698 (PubMed:7659092).Curated1
Sequence conflicti1104K → KQ in AAC52140 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008444614Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01222 mRNA Translation: AAA21643.1
X72711 mRNA Translation: CAA51260.1
U36441 mRNA Translation: AAA79698.1
U07157 mRNA Translation: AAC52140.1
U15037 mRNA Translation: AAB60452.1
CCDSiCCDS39096.1 [P35601-1]
CCDS84884.1 [P35601-2]
PIRiA49393
RefSeqiNP_035388.2, NM_011258.2
UniGeneiMm.148877

Genome annotation databases

GeneIDi19687
KEGGimmu:19687

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01222 mRNA Translation: AAA21643.1
X72711 mRNA Translation: CAA51260.1
U36441 mRNA Translation: AAA79698.1
U07157 mRNA Translation: AAC52140.1
U15037 mRNA Translation: AAB60452.1
CCDSiCCDS39096.1 [P35601-1]
CCDS84884.1 [P35601-2]
PIRiA49393
RefSeqiNP_035388.2, NM_011258.2
UniGeneiMm.148877

3D structure databases

ProteinModelPortaliP35601
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202846, 5 interactors
ComplexPortaliCPX-472 DNA replication factor C complex
CORUMiP35601
STRINGi10090.ENSMUSP00000031092

PTM databases

iPTMnetiP35601
PhosphoSitePlusiP35601
SwissPalmiP35601

Proteomic databases

MaxQBiP35601
PaxDbiP35601
PeptideAtlasiP35601
PRIDEiP35601

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi19687
KEGGimmu:19687

Organism-specific databases

CTDi5981
MGIiMGI:97891 Rfc1

Phylogenomic databases

eggNOGiENOG410IPR8 Eukaryota
COG5275 LUCA
HOVERGENiHBG004167
InParanoidiP35601
KOiK10754
PhylomeDBiP35601

Miscellaneous databases

ChiTaRSiRfc1 mouse
PROiPR:P35601
SOURCEiSearch...

Gene expression databases

CleanExiMM_RFC1

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR013725 DNA_replication_fac_RFC1_C
IPR027417 P-loop_NTPase
IPR012178 RFC1
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF00533 BRCT, 1 hit
PF08519 RFC1, 1 hit
PIRSFiPIRSF036578 RFC1, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00292 BRCT, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52113 SSF52113, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRFC1_MOUSE
AccessioniPrimary (citable) accession number: P35601
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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