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UniProtKB - P35600 (RFC1_DROME)
Protein
Replication factor C subunit 1
Gene
Gnf1
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds to the primer-template junction (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 487 – 494 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- DNA binding Source: FlyBase
- DNA clamp loader activity Source: InterPro
GO - Biological processi
- dendrite morphogenesis Source: FlyBase
- DNA repair Source: InterPro
- DNA replication Source: FlyBase
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | DNA replication, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-110312, Translesion synthesis by REV1 R-DME-110314, Recognition of DNA damage by PCNA-containing replication complex R-DME-110320, Translesion Synthesis by POLH R-DME-5651801, PCNA-Dependent Long Patch Base Excision Repair R-DME-5655862, Translesion synthesis by POLK R-DME-5656121, Translesion synthesis by POLI R-DME-5656169, Termination of translesion DNA synthesis R-DME-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-DME-5696400, Dual Incision in GG-NER R-DME-6782135, Dual incision in TC-NER R-DME-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-DME-69091, Polymerase switching |
SignaLinki | P35600 |
Names & Taxonomyi
Protein namesi | Recommended name: Replication factor C subunit 1Alternative name(s): Activator 1 140 kDa subunit Activator 1 subunit 1 Germline transcription factor 1 Replication factor C large subunit |
Gene namesi | Name:Gnf1 ORF Names:CG1119 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0004913, Gnf1 |
VEuPathDBi | VectorBase:FBgn0004913 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: FlyBase
Other locations
- DNA replication factor C complex Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121774 | 1 – 986 | Replication factor C subunit 1Add BLAST | 986 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | Phosphoserine1 Publication | 1 | |
Modified residuei | 28 | Phosphoserine1 Publication | 1 | |
Modified residuei | 40 | Phosphoserine1 Publication | 1 | |
Modified residuei | 41 | Phosphoserine1 Publication | 1 | |
Modified residuei | 48 | Phosphoserine1 Publication | 1 | |
Modified residuei | 58 | Phosphoserine1 Publication | 1 | |
Modified residuei | 60 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 62 | Phosphoserine1 Publication | 1 | |
Modified residuei | 63 | Phosphoserine1 Publication | 1 | |
Modified residuei | 71 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 128 | Phosphoserine1 Publication | 1 | |
Modified residuei | 137 | Phosphoserine1 Publication | 1 | |
Modified residuei | 149 | Phosphoserine1 Publication | 1 | |
Modified residuei | 154 | Phosphoserine1 Publication | 1 | |
Modified residuei | 156 | Phosphoserine1 Publication | 1 | |
Modified residuei | 164 | Phosphoserine1 Publication | 1 | |
Modified residuei | 194 | Phosphoserine1 Publication | 1 | |
Modified residuei | 197 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 938 | Phosphoserine1 Publication | 1 | |
Modified residuei | 939 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P35600 |
PRIDEi | P35600 |
PTM databases
iPTMneti | P35600 |
Expressioni
Gene expression databases
Bgeei | FBgn0004913, Expressed in embryo and 49 other tissues |
Genevisiblei | P35600, DM |
Interactioni
Subunit structurei
Interacts with C-terminus of PCNA.
1 PublicationProtein-protein interaction databases
BioGRIDi | 65822, 28 interactors |
DIPi | DIP-18735N |
IntActi | P35600, 9 interactors |
STRINGi | 7227.FBpp0099511 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 232 – 322 | BRCTPROSITE-ProRule annotationAdd BLAST | 91 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 95 | DisorderedSequence analysisAdd BLAST | 95 | |
Regioni | 136 – 203 | DisorderedSequence analysisAdd BLAST | 68 | |
Regioni | 317 – 388 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 913 – 986 | DisorderedSequence analysisAdd BLAST | 74 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 955 – 959 | Nuclear localization signalSequence analysis | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 62 | Basic and acidic residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 149 – 175 | Polar residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 178 – 203 | Basic and acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 929 – 958 | Basic and acidic residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 959 – 979 | Polar residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the activator 1 large subunit family.Curated
Phylogenomic databases
eggNOGi | KOG1968, Eukaryota |
GeneTreei | ENSGT00730000111066 |
HOGENOMi | CLU_003574_0_1_1 |
InParanoidi | P35600 |
PhylomeDBi | P35600 |
Family and domain databases
Gene3Di | 3.40.50.10190, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR008921, DNA_pol3_clamp-load_cplx_C IPR013725, DNA_replication_fac_RFC1_C IPR027417, P-loop_NTPase IPR012178, RFC1 |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF00533, BRCT, 1 hit PF08519, RFC1, 1 hit |
PIRSFi | PIRSF036578, RFC1, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00292, BRCT, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52113, SSF52113, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit |
i Sequence
Sequence statusi: Complete.
P35600-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQRGIDSFFK RLPAKAKSAE AENGETPSKA PKRRKAVIIS SDEDEVVSPP
60 70 80 90 100
ETKKRKASKT ASSEDDVVAA TPEPIAKKAR NGQKPALSKL KRHVDPTELF
110 120 130 140 150
GGETKRVIVP KPKTKAVLEF ENEDIDRSLM EVDLDESIKE AAPEKKVHSI
160 170 180 190 200
TRSSPSPKRA KNSSPEPPKP KSTKSKATTP RVKKEKPAAD LESSVLTDEE
210 220 230 240 250
RHERKRASAV LYQKYKNRSS CLNPGSKEIP KGSPDCLSGL TFVVTGVLES
260 270 280 290 300
MEREEAESVI KEYGGKVMTV VGKKLKYLVV GEEAGPKKLA VAEELNIPIL
310 320 330 340 350
SEDGLFDLIR EKSGIAKQVK EEKKSPKKEH SSEEKGKKEV KTSRRSSDKK
360 370 380 390 400
EKEATKLKYG EKHDIAKHKV KEEHTSPKET KDKLNDVPAV TLKVKKEPSS
410 420 430 440 450
QKEHPPSPRT ADLKTLDVVG MAWVDKHKPT SIKEIVGQAG AASNVTKLMN
460 470 480 490 500
WLSKWYVNHD GNKKPQRPNP WAKNDDGSFY KAALLSGPPG IGKTTTATLV
510 520 530 540 550
VKELGFDAVE FNASDTRSKR LLKDEVSTLL SNKSLSGYFT GQGQAVSRKH
560 570 580 590 600
VLIMDEVDGM AGNEDRGGMQ ELIALIKDSS IPIICMCNDR NHPKIRSLVN
610 620 630 640 650
YCYDLRFQRP RLEQIKGKIM SICFKEKVKI SPAKVEEIIA ATNNDIRQSI
660 670 680 690 700
NHIALLSAKE DASQKSGQQV ATKDLKLGPW EVVRKVFTAD EHKHMSFADK
710 720 730 740 750
SDLFFHDYSL APLFVQQNYL QVLPQGNKKD VLAKVAATAD ALSLGDLVEK
760 770 780 790 800
RIRANSAWSL LPTQAFFSSV LPGEHMCGHF TGQINFPGWL GKNSKSGKRA
810 820 830 840 850
RLAQELHDHT RVCTSGSRLS VRLDYAPFLL DNIVRPLAKD GQEGVPAALD
860 870 880 890 900
VMKDYHLLRE DLDSLVELTS WPGKKSPLDA VDGRVKAALT RSYNKEVMAY
910 920 930 940 950
SYSAQAGIKK KKSEAAGADD DYLDEGPGEE DGAGGHLSSE EDEDKDNLEL
960 970 980
DSLIKAKKRT TTSKASGGSK KATSSTASKS KAKAKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 447 | K → NHNLKLKAKQERVKVLHYFN FPR in AAM52589 (Ref. 5) Curated | 1 | |
Sequence conflicti | 559 | G → A in AAA28573 (PubMed:9705493).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L17340 Genomic DNA Translation: AAA28573.1 U97685 mRNA Translation: AAB58311.1 AE014297 Genomic DNA Translation: AAF52082.1 AE014297 Genomic DNA Translation: AAX52937.1 AY122077 mRNA Translation: AAM52589.1 BT003618 mRNA Translation: AAO39621.1 |
RefSeqi | NP_001014605.1, NM_001014605.3 NP_524229.1, NM_079505.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0078837; FBpp0078478; FBgn0004913 |
GeneIDi | 40607 |
KEGGi | dme:Dmel_CG1119 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L17340 Genomic DNA Translation: AAA28573.1 U97685 mRNA Translation: AAB58311.1 AE014297 Genomic DNA Translation: AAF52082.1 AE014297 Genomic DNA Translation: AAX52937.1 AY122077 mRNA Translation: AAM52589.1 BT003618 mRNA Translation: AAO39621.1 |
RefSeqi | NP_001014605.1, NM_001014605.3 NP_524229.1, NM_079505.3 |
3D structure databases
SMRi | P35600 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 65822, 28 interactors |
DIPi | DIP-18735N |
IntActi | P35600, 9 interactors |
STRINGi | 7227.FBpp0099511 |
PTM databases
iPTMneti | P35600 |
Proteomic databases
PaxDbi | P35600 |
PRIDEi | P35600 |
Genome annotation databases
EnsemblMetazoai | FBtr0078837; FBpp0078478; FBgn0004913 |
GeneIDi | 40607 |
KEGGi | dme:Dmel_CG1119 |
Organism-specific databases
CTDi | 100035172 |
FlyBasei | FBgn0004913, Gnf1 |
VEuPathDBi | VectorBase:FBgn0004913 |
Phylogenomic databases
eggNOGi | KOG1968, Eukaryota |
GeneTreei | ENSGT00730000111066 |
HOGENOMi | CLU_003574_0_1_1 |
InParanoidi | P35600 |
PhylomeDBi | P35600 |
Enzyme and pathway databases
Reactomei | R-DME-110312, Translesion synthesis by REV1 R-DME-110314, Recognition of DNA damage by PCNA-containing replication complex R-DME-110320, Translesion Synthesis by POLH R-DME-5651801, PCNA-Dependent Long Patch Base Excision Repair R-DME-5655862, Translesion synthesis by POLK R-DME-5656121, Translesion synthesis by POLI R-DME-5656169, Termination of translesion DNA synthesis R-DME-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-DME-5696400, Dual Incision in GG-NER R-DME-6782135, Dual incision in TC-NER R-DME-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-DME-69091, Polymerase switching |
SignaLinki | P35600 |
Miscellaneous databases
BioGRID-ORCSi | 40607, 1 hit in 1 CRISPR screen |
GenomeRNAii | 40607 |
PROi | PR:P35600 |
Gene expression databases
Bgeei | FBgn0004913, Expressed in embryo and 49 other tissues |
Genevisiblei | P35600, DM |
Family and domain databases
Gene3Di | 3.40.50.10190, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR008921, DNA_pol3_clamp-load_cplx_C IPR013725, DNA_replication_fac_RFC1_C IPR027417, P-loop_NTPase IPR012178, RFC1 |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF00533, BRCT, 1 hit PF08519, RFC1, 1 hit |
PIRSFi | PIRSF036578, RFC1, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00292, BRCT, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52113, SSF52113, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RFC1_DROME | |
Accessioni | P35600Primary (citable) accession number: P35600 Secondary accession number(s): O02031, Q8MR76 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families