UniProtKB - P35584 (FOXA3_MOUSE)
Protein
Hepatocyte nuclear factor 3-gamma
Gene
Foxa3
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; activates GLUT2 transcription. Involved in regulation of neuronal-specific transcription. Involved in regulation of spermatogenesis; required for the maintenance of the testicular germ cell population and male fertility.By similarity3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 118 – 209 | Fork-headPROSITE-ProRule annotationAdd BLAST | 92 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein domain specific binding Source: InterPro
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: InterPro
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- anatomical structure morphogenesis Source: GO_Central
- cell differentiation Source: GO_Central
- cellular glucose homeostasis Source: MGI
- cellular response to starvation Source: MGI
- chromatin organization Source: UniProtKB-KW
- endocrine pancreas development Source: Reactome
- negative regulation of cell population proliferation Source: MGI
- positive regulation of hepatocyte differentiation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: UniProtKB
- spermatogenesis Source: UniProtKB
Keywordsi
Molecular function | Activator, Chromatin regulator, Developmental protein, DNA-binding |
Biological process | Differentiation, Spermatogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Hepatocyte nuclear factor 3-gammaShort name: HNF-3-gamma Short name: HNF-3G Alternative name(s): Forkhead box protein A3 |
Gene namesi | Name:Foxa3 Synonyms:Hnf3g, Tcf-3g, Tcf3g |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1347477, Foxa3 |
Subcellular locationi
Nucleus
Cytoskeleton
- actin cytoskeleton Source: MGI
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Reduced expression levels of several HNF3 target genes (phosphoenolpyruvate carboxykinase, transferrin, tyrosine aminotransferase) by 50 to 70%,. The germinal epithelium of testes is characterized by a loss of germ cells secondary to an increase in germ cell apoptosis that ultimately leads to a Sertoli cell-only syndrome. Significantly lower blood glucose in fasted mice.3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091801 | 1 – 353 | Hepatocyte nuclear factor 3-gammaAdd BLAST | 353 |
Proteomic databases
MaxQBi | P35584 |
PaxDbi | P35584 |
PeptideAtlasi | P35584 |
PRIDEi | P35584 |
PTM databases
iPTMneti | P35584 |
PhosphoSitePlusi | P35584 |
Expressioni
Tissue specificityi
Restricted mainly to endoderm-derived tissues (lung, liver, stomach, and small intestine), also present additionally in ovary, testis, heart, and adipose tissue, but missing from lung.1 Publication
Developmental stagei
Expression peaks around day 15.5 of gestation. Expressed from day 6 to day 70 during postnatal testicular development.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000040891, Expressed in mucous cell of stomach and 126 other tissues |
Genevisiblei | P35584, MM |
Interactioni
Subunit structurei
Interacts with FOXA2.
By similarityGO - Molecular functioni
- protein domain specific binding Source: InterPro
- transcription factor binding Source: InterPro
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000043173 |
Miscellaneous databases
RNActi | P35584, protein |
Family & Domainsi
Phylogenomic databases
eggNOGi | KOG3563, Eukaryota |
GeneTreei | ENSGT00940000162453 |
HOGENOMi | CLU_027910_0_0_1 |
InParanoidi | P35584 |
OMAi | DPHYNFN |
OrthoDBi | 1181467at2759 |
PhylomeDBi | P35584 |
TreeFami | TF316127 |
Family and domain databases
CDDi | cd00059, FH, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR013638, Fork-head_N IPR001766, Fork_head_dom IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00250, Forkhead, 1 hit PF08430, Forkhead_N, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P35584-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLGSVKMEAH DLAEWSYYPE AGEVYSPVNP VPTMAPLNSY MTLNPLSSPY
60 70 80 90 100
PPGGLQASPL PTGPLAPPAP TAPLGPTFPS LGTGGSTGGS ASGYVAPGPG
110 120 130 140 150
LVHGKEMAKG YRRPLAHAKP PYSYISLITM AIQQAPGKML TLSEIYQWIM
160 170 180 190 200
DLFPYYRENQ QRWQNSIRHS LSFNDCFVKV ARSPDKPGKG SYWALHPSSG
210 220 230 240 250
NMFENGCYLR RQKRFKLEEK AKKGNSATSA SRNGTAGSAT SATTTAATAV
260 270 280 290 300
TSPAQPQPTP SEPEAQSGDD VGGLDCASPP SSTPYFSGLE LPGELKLDAP
310 320 330 340 350
YNFNHPFSIN NLMSEQTSTP SKLDVGFGGY GAESGEPGVY YQSLYSRSLL
NAS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74938 mRNA Translation: CAA52892.1 |
CCDSi | CCDS20886.1 |
PIRi | C54258 |
RefSeqi | NP_032286.1, NM_008260.2 |
Genome annotation databases
Ensembli | ENSMUST00000036018; ENSMUSP00000043173; ENSMUSG00000040891 |
GeneIDi | 15377 |
KEGGi | mmu:15377 |
UCSCi | uc009fkd.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74938 mRNA Translation: CAA52892.1 |
CCDSi | CCDS20886.1 |
PIRi | C54258 |
RefSeqi | NP_032286.1, NM_008260.2 |
3D structure databases
SMRi | P35584 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000043173 |
PTM databases
iPTMneti | P35584 |
PhosphoSitePlusi | P35584 |
Proteomic databases
MaxQBi | P35584 |
PaxDbi | P35584 |
PeptideAtlasi | P35584 |
PRIDEi | P35584 |
Protocols and materials databases
Antibodypediai | 4191, 92 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000036018; ENSMUSP00000043173; ENSMUSG00000040891 |
GeneIDi | 15377 |
KEGGi | mmu:15377 |
UCSCi | uc009fkd.1, mouse |
Organism-specific databases
CTDi | 3171 |
MGIi | MGI:1347477, Foxa3 |
Phylogenomic databases
eggNOGi | KOG3563, Eukaryota |
GeneTreei | ENSGT00940000162453 |
HOGENOMi | CLU_027910_0_0_1 |
InParanoidi | P35584 |
OMAi | DPHYNFN |
OrthoDBi | 1181467at2759 |
PhylomeDBi | P35584 |
TreeFami | TF316127 |
Miscellaneous databases
BioGRID-ORCSi | 15377, 4 hits in 17 CRISPR screens |
ChiTaRSi | Foxa3, mouse |
PROi | PR:P35584 |
RNActi | P35584, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040891, Expressed in mucous cell of stomach and 126 other tissues |
Genevisiblei | P35584, MM |
Family and domain databases
CDDi | cd00059, FH, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR013638, Fork-head_N IPR001766, Fork_head_dom IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00250, Forkhead, 1 hit PF08430, Forkhead_N, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FOXA3_MOUSE | |
Accessioni | P35584Primary (citable) accession number: P35584 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot