UniProtKB - P35563 (5HT3A_RAT)
Protein
5-hydroxytryptamine receptor 3A
Gene
Htr3a
Organism
Rattus norvegicus (Rat)
Status
Functioni
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication
Miscellaneous
The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.
GO - Molecular functioni
- identical protein binding Source: RGD
- neurotransmitter receptor activity Source: GO_Central
- serotonin binding Source: RGD
- serotonin-gated cation-selective channel activity Source: RGD
- transmembrane signaling receptor activity Source: InterPro
GO - Biological processi
- cellular response to growth factor stimulus Source: RGD
- chemical synaptic transmission Source: GO_Central
- ion transmembrane transport Source: GO_Central
- nervous system process Source: GO_Central
- positive regulation of ion transmembrane transporter activity Source: RGD
- regulation of membrane potential Source: GO_Central
- response to cocaine Source: RGD
- response to ethanol Source: RGD
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-112314, Neurotransmitter receptors and postsynaptic signal transmission |
Protein family/group databases
TCDBi | 1.A.9.2.2, the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family |
Names & Taxonomyi
Protein namesi | Recommended name: 5-hydroxytryptamine receptor 3AShort name: 5-HT3-A Short name: 5-HT3A Alternative name(s): 5-hydroxytryptamine receptor 3 Short name: 5-HT-3 Short name: 5-HT3R Serotonin receptor 3A Serotonin-gated ion channel receptor |
Gene namesi | Name:Htr3a Synonyms:5ht3, Htr3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61818, Htr3a |
Subcellular locationi
Plasma Membrane
- cleavage furrow Source: RGD
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic membrane Source: SynGO
- integral component of presynaptic membrane Source: SynGO
- plasma membrane Source: RGD
- serotonin-activated cation-selective channel complex Source: RGD
Other locations
- axon Source: RGD
- cytoplasm Source: RGD
- glutamatergic synapse Source: SynGO
- neuron projection Source: GO_Central
- neuronal cell body Source: RGD
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 246 | ExtracellularSequence analysisAdd BLAST | 223 | |
Transmembranei | 247 – 273 | Helical; Name=1Sequence analysisAdd BLAST | 27 | |
Topological domaini | 274 – 278 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 279 – 297 | Helical; Name=2Sequence analysisAdd BLAST | 19 | |
Topological domaini | 298 – 307 | ExtracellularSequence analysis | 10 | |
Transmembranei | 308 – 326 | Helical; Name=3Sequence analysisAdd BLAST | 19 | |
Topological domaini | 327 – 460 | CytoplasmicSequence analysisAdd BLAST | 134 | |
Transmembranei | 461 – 480 | Helical; Name=4Sequence analysisAdd BLAST | 20 | |
Topological domaini | 481 – 483 | ExtracellularSequence analysis | 3 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL2411 |
DrugCentrali | P35563 |
GuidetoPHARMACOLOGYi | 373 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000000410 | 24 – 483 | 5-hydroxytryptamine receptor 3AAdd BLAST | 460 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 109 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 162 ↔ 176 | By similarity | ||
Glycosylationi | 175 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 191 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P35563 |
PRIDEi | P35563 |
PTM databases
GlyGeni | P35563, 3 sites |
PhosphoSitePlusi | P35563 |
Expressioni
Tissue specificityi
Expressed in central and peripheral neurons.1 Publication
Inductioni
By nerve growth factor in PC12 cells.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000006595, Expressed in Ammon's horn and 10 other tissues |
Genevisiblei | P35563, RN |
Interactioni
Subunit structurei
Interacts with RIC3 (By similarity). Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence and antagonist affinity.
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
ComplexPortali | CPX-277, 5-hydroxytryptamine-3A/B receptor complex CPX-278, 5-hydroxytryptamine-3A receptor complex |
STRINGi | 10116.ENSRNOP00000008965 |
Chemistry databases
BindingDBi | P35563 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 419 – 455 | HA-stretchAdd BLAST | 37 |
Sequence similaritiesi
Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. [View classification]Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000157705 |
HOGENOMi | CLU_018074_5_0_1 |
InParanoidi | P35563 |
OMAi | PYVYVTH |
OrthoDBi | 611108at2759 |
PhylomeDBi | P35563 |
TreeFami | TF315605 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR008132, 5HT3_rcpt IPR008133, 5HT3_rcpt_A IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01709, 5HT3ARECEPTR PR01708, 5HT3RECEPTOR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P35563-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPLCIPQVLL ALFLSVLIAQ GEGSRRRATQ AHSTTQPALL RLSDHLLANY
60 70 80 90 100
KKGVRPVRDW RKPTLVSIDV IMYAILNVDE KNQVLTTYIW YRQFWTDEFL
110 120 130 140 150
QWTPEDFDNV TKLSIPTDSI WVPDILINEF VDVGKSPSIP YVYVHHQGEV
160 170 180 190 200
QNYKPLQLVT ACSLDIYNFP FDVQNCSLTF TSWLHTIQDI NISLWRTPEE
210 220 230 240 250
VRSDKSIFIN QGEWELLGVF TKFQEFSIET SNSYAEMKFY VVIRRRPLFY
260 270 280 290 300
AVSLLLPSIF LMVVDIVGFC LPPDSGERVS FKITLLLGYS VFLIIVSDTL
310 320 330 340 350
PATAIGTPLI GVYFVVCMAL LVISLAETIF IVQLVHKQDL QRPVPDWLRH
360 370 380 390 400
LVLDRIAWLL CLGEQPMAHR PPATFQANKT DDCSAMGNHC SHVGSPQDLE
410 420 430 440 450
KTSRSRDSPL PPPREASLAV RGLLQELSSI RHSLEKRDEM REVARDWLRV
460 470 480
GYVLDRLLFR IYLLAVLAYS ITLVTLWSIW HYS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 | E → K in AAA52182 (PubMed:7509203).Curated | 1 | |
Sequence conflicti | 306 | G → R in AAA52182 (PubMed:7509203).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49395 mRNA Translation: BAA08388.1 U01227 mRNA Translation: AAA52182.1 |
PIRi | I58179 |
RefSeqi | NP_077370.2, NM_024394.2 |
Genome annotation databases
Ensembli | ENSRNOT00000008965; ENSRNOP00000008965; ENSRNOG00000006595 |
GeneIDi | 79246 |
KEGGi | rno:79246 |
UCSCi | RGD:61818, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49395 mRNA Translation: BAA08388.1 U01227 mRNA Translation: AAA52182.1 |
PIRi | I58179 |
RefSeqi | NP_077370.2, NM_024394.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
ComplexPortali | CPX-277, 5-hydroxytryptamine-3A/B receptor complex CPX-278, 5-hydroxytryptamine-3A receptor complex |
STRINGi | 10116.ENSRNOP00000008965 |
Chemistry databases
BindingDBi | P35563 |
ChEMBLi | CHEMBL2411 |
DrugCentrali | P35563 |
GuidetoPHARMACOLOGYi | 373 |
Protein family/group databases
TCDBi | 1.A.9.2.2, the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family |
PTM databases
GlyGeni | P35563, 3 sites |
PhosphoSitePlusi | P35563 |
Proteomic databases
PaxDbi | P35563 |
PRIDEi | P35563 |
Genome annotation databases
Ensembli | ENSRNOT00000008965; ENSRNOP00000008965; ENSRNOG00000006595 |
GeneIDi | 79246 |
KEGGi | rno:79246 |
UCSCi | RGD:61818, rat |
Organism-specific databases
CTDi | 3359 |
RGDi | 61818, Htr3a |
Phylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000157705 |
HOGENOMi | CLU_018074_5_0_1 |
InParanoidi | P35563 |
OMAi | PYVYVTH |
OrthoDBi | 611108at2759 |
PhylomeDBi | P35563 |
TreeFami | TF315605 |
Enzyme and pathway databases
Reactomei | R-RNO-112314, Neurotransmitter receptors and postsynaptic signal transmission |
Miscellaneous databases
PROi | PR:P35563 |
Gene expression databases
Bgeei | ENSRNOG00000006595, Expressed in Ammon's horn and 10 other tissues |
Genevisiblei | P35563, RN |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR008132, 5HT3_rcpt IPR008133, 5HT3_rcpt_A IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01709, 5HT3ARECEPTR PR01708, 5HT3RECEPTOR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 5HT3A_RAT | |
Accessioni | P35563Primary (citable) accession number: P35563 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families