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Protein

5-hydroxytryptamine receptor 3A

Gene

Htr3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

Miscellaneous

The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.

GO - Molecular functioni

GO - Biological processi

  • cellular response to growth factor stimulus Source: RGD
  • chemical synaptic transmission Source: RGD
  • positive regulation of ion transmembrane transporter activity Source: RGD
  • response to cocaine Source: RGD
  • response to ethanol Source: RGD

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-112314 Neurotransmitter receptors and postsynaptic signal transmission

Protein family/group databases

TCDBi1.A.9.2.2 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3A
Short name:
5-HT3-A
Short name:
5-HT3A
Alternative name(s):
5-hydroxytryptamine receptor 3
Short name:
5-HT-3
Short name:
5-HT3R
Serotonin receptor 3A
Serotonin-gated ion channel receptor
Gene namesi
Name:Htr3a
Synonyms:5ht3, Htr3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi61818 Htr3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 246ExtracellularSequence analysisAdd BLAST223
Transmembranei247 – 273Helical; Name=1Sequence analysisAdd BLAST27
Topological domaini274 – 278CytoplasmicSequence analysis5
Transmembranei279 – 297Helical; Name=2Sequence analysisAdd BLAST19
Topological domaini298 – 307ExtracellularSequence analysis10
Transmembranei308 – 326Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini327 – 460CytoplasmicSequence analysisAdd BLAST134
Transmembranei461 – 480Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini481 – 483ExtracellularSequence analysis3

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2411
GuidetoPHARMACOLOGYi373

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000041024 – 4835-hydroxytryptamine receptor 3AAdd BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi162 ↔ 176By similarity
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi191N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP35563

PTM databases

PhosphoSitePlusiP35563

Expressioni

Tissue specificityi

Expressed in central and peripheral neurons.1 Publication

Inductioni

By nerve growth factor in PC12 cells.1 Publication

Gene expression databases

BgeeiENSRNOG00000006595
GenevisibleiP35563 RN

Interactioni

Subunit structurei

Interacts with RIC3 (By similarity). Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence and antagonist affinity.By similarity2 Publications

Protein-protein interaction databases

ComplexPortaliCPX-277 5-hydroxytryptamine-3A/B receptor complex
CPX-278 5-hydroxytryptamine-3A receptor complex
STRINGi10116.ENSRNOP00000008965

Chemistry databases

BindingDBiP35563

Structurei

3D structure databases

ProteinModelPortaliP35563
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni419 – 455HA-stretchAdd BLAST37

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00910000144023
HOGENOMiHOG000241519
HOVERGENiHBG106638
InParanoidiP35563
KOiK04819
OMAiLWSIWQY
OrthoDBiEOG091G06Q8
PhylomeDBiP35563
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR008132 5HT3_rcpt
IPR008133 5HT3_rcpt_A
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01709 5HT3ARECEPTR
PR01708 5HT3RECEPTOR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLCIPQVLL ALFLSVLIAQ GEGSRRRATQ AHSTTQPALL RLSDHLLANY
60 70 80 90 100
KKGVRPVRDW RKPTLVSIDV IMYAILNVDE KNQVLTTYIW YRQFWTDEFL
110 120 130 140 150
QWTPEDFDNV TKLSIPTDSI WVPDILINEF VDVGKSPSIP YVYVHHQGEV
160 170 180 190 200
QNYKPLQLVT ACSLDIYNFP FDVQNCSLTF TSWLHTIQDI NISLWRTPEE
210 220 230 240 250
VRSDKSIFIN QGEWELLGVF TKFQEFSIET SNSYAEMKFY VVIRRRPLFY
260 270 280 290 300
AVSLLLPSIF LMVVDIVGFC LPPDSGERVS FKITLLLGYS VFLIIVSDTL
310 320 330 340 350
PATAIGTPLI GVYFVVCMAL LVISLAETIF IVQLVHKQDL QRPVPDWLRH
360 370 380 390 400
LVLDRIAWLL CLGEQPMAHR PPATFQANKT DDCSAMGNHC SHVGSPQDLE
410 420 430 440 450
KTSRSRDSPL PPPREASLAV RGLLQELSSI RHSLEKRDEM REVARDWLRV
460 470 480
GYVLDRLLFR IYLLAVLAYS ITLVTLWSIW HYS
Length:483
Mass (Da):55,428
Last modified:November 1, 1995 - v2
Checksum:iED85257BBCCF28A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22E → K in AAA52182 (PubMed:7509203).Curated1
Sequence conflicti306G → R in AAA52182 (PubMed:7509203).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49395 mRNA Translation: BAA08388.1
U01227 mRNA Translation: AAA52182.1
PIRiI58179
RefSeqiNP_077370.2, NM_024394.2
UniGeneiRn.55109

Genome annotation databases

EnsembliENSRNOT00000008965; ENSRNOP00000008965; ENSRNOG00000006595
GeneIDi79246
KEGGirno:79246
UCSCiRGD:61818 rat

Similar proteinsi

Entry informationi

Entry namei5HT3A_RAT
AccessioniPrimary (citable) accession number: P35563
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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