Htr3a

Functionⁱ

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

Miscellaneous

The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.

GO - Molecular functionⁱ

serotonin binding Source: Ensembl
serotonin-gated cation-selective channel activity
Source: RGD
Inferred from direct assayⁱ
- 12196560
transmembrane signaling receptor activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to growth factor stimulus
Source: RGD
Inferred from expression patternⁱ
- 10854267
chemical synaptic transmission
Source: RGD
Traceable author statementⁱ
- 12196560
positive regulation of ion transmembrane transporter activity
Source: RGD
Inferred from direct assayⁱ
- 10854267
response to cocaine
Source: RGD
Inferred from expression patternⁱ
- 17105936
response to ethanol
Source: RGD
Inferred from expression patternⁱ
- 16876304

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ion channel, Ligand-gated ion channel, Receptor
Biological process	Ion transport, Transport

Enzyme and pathway databases

Reactomeⁱ	R-RNO-112314 Neurotransmitter receptors and postsynaptic signal transmission

Reactomeⁱ

R-RNO-112314 Neurotransmitter receptors and postsynaptic signal transmission

Protein family/group databases

TCDBⁱ	1.A.9.2.2 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

TCDBⁱ

1.A.9.2.2 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 5-hydroxytryptamine receptor 3A Short name: 5-HT3-A Short name: 5-HT3A Alternative name(s): 5-hydroxytryptamine receptor 3 Short name: 5-HT-3 Short name: 5-HT3R Serotonin receptor 3A Serotonin-gated ion channel receptor
Gene namesⁱ	Name:Htr3a Synonyms:5ht3, Htr3
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 8

Organism-specific databases

Rat genome database More...RGDⁱ	61818 Htr3a

RGDⁱ

61818 Htr3a

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	24 – 246	ExtracellularSequence analysisAdd BLAST	223
Transmembraneⁱ	247 – 273	Helical; Name=1Sequence analysisAdd BLAST	27
Topological domainⁱ	274 – 278	CytoplasmicSequence analysis	5
Transmembraneⁱ	279 – 297	Helical; Name=2Sequence analysisAdd BLAST	19
Topological domainⁱ	298 – 307	ExtracellularSequence analysis	10
Transmembraneⁱ	308 – 326	Helical; Name=3Sequence analysisAdd BLAST	19
Topological domainⁱ	327 – 460	CytoplasmicSequence analysisAdd BLAST	134
Transmembraneⁱ	461 – 480	Helical; Name=4Sequence analysisAdd BLAST	20
Topological domainⁱ	481 – 483	ExtracellularSequence analysis	3

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL2411
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	373

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 23	Sequence analysisAdd BLAST		23
ChainⁱPRO_0000000410	24 – 483	5-hydroxytryptamine receptor 3AAdd BLAST		460

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	109	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	162 ↔ 176	By similarity
Glycosylationⁱ	175	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	191	N-linked (GlcNAc...) asparagineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	P35563

PaxDbⁱ

P35563

PTM databases

PhosphoSitePlusⁱ	P35563

PhosphoSitePlusⁱ

P35563

Expressionⁱ

Tissue specificityⁱ

Expressed in central and peripheral neurons.1 Publication

Inductionⁱ

By nerve growth factor in PC12 cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSRNOG00000006595 Expressed in 4 organ(s), highest expression level in brain
Genevisibleⁱ	P35563 RN

Interactionⁱ

Subunit structureⁱ

Interacts with RIC3 (By similarity). Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence and antagonist affinity.By similarity2 Publications

Protein-protein interaction databases

ComplexPortalⁱ	CPX-277 5-hydroxytryptamine-3A/B receptor complex CPX-278 5-hydroxytryptamine-3A receptor complex
STRINGⁱ	10116.ENSRNOP00000008965

Chemistry databases

BindingDBⁱ

P35563

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P35563
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	419 – 455	HA-stretchAdd BLAST		37

Sequence similaritiesⁱ

Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. [View classification]Curated

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG3645 Eukaryota ENOG410XQGR LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144023
HOGENOMⁱ	HOG000241519
HOVERGENⁱ	HBG106638
InParanoidⁱ	P35563
KEGG Orthology (KO) More...KOⁱ	K04819
OMAⁱ	LWSIWQY
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06Q8
PhylomeDBⁱ	P35563
TreeFamⁱ	TF315605

Family and domain databases

Gene3Dⁱ	2.70.170.10, 1 hit
InterProⁱ	View protein in InterPro IPR008132 5HT3_rcpt IPR008133 5HT3_rcpt_A IPR006202 Neur_chan_lig-bd IPR036734 Neur_chan_lig-bd_sf IPR006201 Neur_channel IPR036719 Neuro-gated_channel_TM_sf IPR006029 Neurotrans-gated_channel_TM IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERⁱ	PTHR18945 PTHR18945, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02931 Neur_chan_LBD, 1 hit PF02932 Neur_chan_memb, 1 hit
PRINTSⁱ	PR01709 5HT3ARECEPTR PR01708 5HT3RECEPTOR PR00252 NRIONCHANNEL
SUPFAMⁱ	SSF63712 SSF63712, 1 hit SSF90112 SSF90112, 1 hit
TIGRFAMsⁱ	TIGR00860 LIC, 1 hit
PROSITEⁱ	View protein in PROSITE PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P35563-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPLCIPQVLL ALFLSVLIAQ GEGSRRRATQ AHSTTQPALL RLSDHLLANY 
        60         70         80         90        100
KKGVRPVRDW RKPTLVSIDV IMYAILNVDE KNQVLTTYIW YRQFWTDEFL 
       110        120        130        140        150
QWTPEDFDNV TKLSIPTDSI WVPDILINEF VDVGKSPSIP YVYVHHQGEV 
       160        170        180        190        200
QNYKPLQLVT ACSLDIYNFP FDVQNCSLTF TSWLHTIQDI NISLWRTPEE 
       210        220        230        240        250
VRSDKSIFIN QGEWELLGVF TKFQEFSIET SNSYAEMKFY VVIRRRPLFY 
       260        270        280        290        300
AVSLLLPSIF LMVVDIVGFC LPPDSGERVS FKITLLLGYS VFLIIVSDTL 
       310        320        330        340        350
PATAIGTPLI GVYFVVCMAL LVISLAETIF IVQLVHKQDL QRPVPDWLRH 
       360        370        380        390        400
LVLDRIAWLL CLGEQPMAHR PPATFQANKT DDCSAMGNHC SHVGSPQDLE 
       410        420        430        440        450
KTSRSRDSPL PPPREASLAV RGLLQELSSI RHSLEKRDEM REVARDWLRV 
       460        470        480 
GYVLDRLLFR IYLLAVLAYS ITLVTLWSIW HYS

Length:483

Mass (Da):55,428

Last modified:November 1, 1995 - v2

Checksum:ⁱED85257BBCCF28A4

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	22	E → K in AAA52182 (PubMed:7509203).Curated		1
Sequence conflictⁱ	306	G → R in AAA52182 (PubMed:7509203).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D49395 mRNA Translation: BAA08388.1 U01227 mRNA Translation: AAA52182.1
PIRⁱ	I58179
NCBI Reference Sequences More...RefSeqⁱ	NP_077370.2, NM_024394.2
UniGeneⁱ	Rn.55109

Genome annotation databases

Ensemblⁱ	ENSRNOT00000008965; ENSRNOP00000008965; ENSRNOG00000006595
GeneIDⁱ	79246
KEGGⁱ	rno:79246
UCSC genome browser More...UCSCⁱ	RGD:61818 rat

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P35563	5HT3A serotonin receptor subunit short isoform (Fragment)		RAT		69	UniRef100_P35563

Protein	Similar proteins		Species	Score	Length	Source
P35563	5-hydroxytryptamine receptor 3A	)	MOUSE		487	UniRef90_P35563
5HT3A serotonin receptor subunit short isoform (Fragment)		RAT		69
5-hydroxytryptamine receptor 3A		MESAU		483
Uncharacterized protein (Fragment)		NEOLE		471
UPI00067E0A99		MICOH		505
+22

Protein	Similar proteins		Species	Score	Length	Source
P35563	5-hydroxytryptamine receptor 3A		HUMAN		478	UniRef50_P46098
5-hydroxytryptamine receptor 3A	)	MOUSE		487
5-hydroxytryptamine receptor 3A		CAVPO		490
Uncharacterized protein		AOTNA		489
Uncharacterized protein		MANLE		489
+412

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D49395 mRNA Translation: BAA08388.1 U01227 mRNA Translation: AAA52182.1
PIRⁱ	I58179
RefSeqⁱ	NP_077370.2, NM_024394.2
UniGeneⁱ	Rn.55109

3D structure databases

ProteinModelPortalⁱ	P35563
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-277 5-hydroxytryptamine-3A/B receptor complex CPX-278 5-hydroxytryptamine-3A receptor complex
STRINGⁱ	10116.ENSRNOP00000008965

Chemistry databases

BindingDBⁱ	P35563
ChEMBLⁱ	CHEMBL2411
GuidetoPHARMACOLOGYⁱ	373

Protein family/group databases

TCDBⁱ	1.A.9.2.2 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

TCDBⁱ

1.A.9.2.2 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

PTM databases

PhosphoSitePlusⁱ	P35563

PhosphoSitePlusⁱ

P35563

Proteomic databases

PaxDbⁱ	P35563

PaxDbⁱ

P35563

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000008965; ENSRNOP00000008965; ENSRNOG00000006595
GeneIDⁱ	79246
KEGGⁱ	rno:79246
UCSCⁱ	RGD:61818 rat

Organism-specific databases

CTDⁱ	3359
RGDⁱ	61818 Htr3a

Phylogenomic databases

eggNOGⁱ	KOG3645 Eukaryota ENOG410XQGR LUCA
GeneTreeⁱ	ENSGT00910000144023
HOGENOMⁱ	HOG000241519
HOVERGENⁱ	HBG106638
InParanoidⁱ	P35563
KOⁱ	K04819
OMAⁱ	LWSIWQY
OrthoDBⁱ	EOG091G06Q8
PhylomeDBⁱ	P35563
TreeFamⁱ	TF315605

Enzyme and pathway databases

Reactomeⁱ	R-RNO-112314 Neurotransmitter receptors and postsynaptic signal transmission

Reactomeⁱ

R-RNO-112314 Neurotransmitter receptors and postsynaptic signal transmission

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P35563

PROⁱ

PR:P35563

Gene expression databases

Bgeeⁱ	ENSRNOG00000006595 Expressed in 4 organ(s), highest expression level in brain
Genevisibleⁱ	P35563 RN

Family and domain databases

Gene3Dⁱ	2.70.170.10, 1 hit
InterProⁱ	View protein in InterPro IPR008132 5HT3_rcpt IPR008133 5HT3_rcpt_A IPR006202 Neur_chan_lig-bd IPR036734 Neur_chan_lig-bd_sf IPR006201 Neur_channel IPR036719 Neuro-gated_channel_TM_sf IPR006029 Neurotrans-gated_channel_TM IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERⁱ	PTHR18945 PTHR18945, 1 hit
Pfamⁱ	View protein in Pfam PF02931 Neur_chan_LBD, 1 hit PF02932 Neur_chan_memb, 1 hit
PRINTSⁱ	PR01709 5HT3ARECEPTR PR01708 5HT3RECEPTOR PR00252 NRIONCHANNEL
SUPFAMⁱ	SSF63712 SSF63712, 1 hit SSF90112 SSF90112, 1 hit
TIGRFAMsⁱ	TIGR00860 LIC, 1 hit
PROSITEⁱ	View protein in PROSITE PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	5HT3A_RAT
Accessionⁱ	P35563Primary (citable) accession number: P35563
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
	Last sequence update:	November 1, 1995
	Last modified:	September 12, 2018
	This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniProtKB - P35563 (5HT3A_RAT)

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5-hydroxytryptamine receptor 3A

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ