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Protein

rRNA 2'-O-methyltransferase fibrillarin

Gene

fib1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N5-methyl-L-glutamine-[histone].

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Ribonucleoprotein, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA 2'-O-methyltransferase fibrillarin (EC:2.1.1.-)
Alternative name(s):
Histone-glutamine methyltransferase
Gene namesi
Name:fib1
Synonyms:fib
ORF Names:SPBC2D10.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC2D10.10c
PomBaseiSPBC2D10.10c fib1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485261 – 305rRNA 2'-O-methyltransferase fibrillarinAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphoserine1 Publication1
Modified residuei114Phosphoserine1 Publication1

Post-translational modificationi

By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35551
PaxDbiP35551
PRIDEiP35551

PTM databases

iPTMnetiP35551

Interactioni

Subunit structurei

Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles.By similarity

Protein-protein interaction databases

BioGridi277007, 9 interactors
STRINGi4896.SPBC2D10.10c.1

Structurei

3D structure databases

ProteinModelPortaliP35551
SMRiP35551
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 161S-adenosyl-L-methionine bindingBy similarity2
Regioni179 – 180S-adenosyl-L-methionine bindingBy similarity2
Regioni204 – 205S-adenosyl-L-methionine bindingBy similarity2
Regioni224 – 227S-adenosyl-L-methionine bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 70DMA/Gly-richAdd BLAST65

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000106741
InParanoidiP35551
KOiK14563
OMAiQPNQAEI
OrthoDBiEOG092C4CCH
PhylomeDBiP35551

Family and domain databases

HAMAPiMF_00351 RNA_methyltransf_FlpA, 1 hit
InterProiView protein in InterPro
IPR000692 Fibrillarin
IPR020813 Fibrillarin_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01269 Fibrillarin, 1 hit
PIRSFiPIRSF006540 Nop17p, 1 hit
PRINTSiPR00052 FIBRILLARIN
SMARTiView protein in SMART
SM01206 Fibrillarin, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS00566 FIBRILLARIN, 1 hit

Sequencei

Sequence statusi: Complete.

P35551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYTPGSRGG RGGSRGGRGG FNGGRGGFGG GRGGARGGGR GGARGGRGGR
60 70 80 90 100
GGARGGRGGS SGGRGGAKGG AKVIIEPHRH AGVFIARGKE DLLVTRNLVP
110 120 130 140 150
GESVYNEKRI SVDSPDGTKV EYRVWNPFRS KLAAGILGGL DNIYIKPGAR
160 170 180 190 200
VLYLGAANGT SVSHVADVVG PEGLVYAVEF SHRSGRDLLN MAKKRTNVIP
210 220 230 240 250
IVEDARHVQK YRMLVGMVDV VFADVAQPDQ ARIVALNAAA FLKNEGGVVI
260 270 280 290 300
SVKASCIDST ADAAVVFARE VKKMQEEKIK PQEQLTLEPY ERDHCIIVGK

YLRHQ
Length:305
Mass (Da):32,040
Last modified:June 1, 1994 - v1
Checksum:i5AE6E8B37EC33331
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69930 Genomic DNA Translation: CAA49550.1
CU329671 Genomic DNA Translation: CAA21168.1
AB027941 Genomic DNA Translation: BAA87245.1
PIRiS33690
RefSeqiNP_596229.1, NM_001022149.2

Genome annotation databases

EnsemblFungiiSPBC2D10.10c.1; SPBC2D10.10c.1:pep; SPBC2D10.10c
GeneIDi2540479
KEGGispo:SPBC2D10.10c

Similar proteinsi

Entry informationi

Entry nameiFBRL_SCHPO
AccessioniPrimary (citable) accession number: P35551
Secondary accession number(s): Q9UTX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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