UniProtKB - P35494 (PMGI_TOBAC)
Protein
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Functioni
Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.By similarity
Catalytic activityi
- EC:5.4.2.12
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
: glycolysis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC)
- Phosphoglycerate kinase, cytosolic
- Phosphoglycerate mutase (2,3-diphosphoglycerate-independent) (LOC107831677), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, Phosphoglycerate mutase (2,3-diphosphoglycerate-independent) (LOC107769473)
- Phosphopyruvate hydratase (LOC107792078), Phosphopyruvate hydratase (LOC107832416), Phosphopyruvate hydratase (LOC107806838), Phosphopyruvate hydratase (LOC107781796), Phosphopyruvate hydratase (LOC107778291), Phosphopyruvate hydratase, Phosphopyruvate hydratase (LOC107796735), Phosphopyruvate hydratase (LOC107779604), Phosphopyruvate hydratase (LOC107782995), Phosphopyruvate hydratase (LOC107776643), Phosphopyruvate hydratase (LOC107808060), Phosphopyruvate hydratase (LOC107808060)
- Pyruvate kinase (LOC107803087), Pyruvate kinase (LOC107824278), Pyruvate kinase (LOC107761670), Pyruvate kinase (LOC107807979), Pyruvate kinase (LOC107830802), Pyruvate kinase (LOC107830802), Pyruvate kinase, cytosolic isozyme, Pyruvate kinase (LOC107787533), Pyruvate kinase (LOC107797877), Pyruvate kinase (LOC107778911), Pyruvate kinase (LOC107821513), Pyruvate kinase (LOC107830388), Pyruvate kinase (LOC107794535), Pyruvate kinase (LOC107820699), Pyruvate kinase (LOC107830388), Pyruvate kinase (LOC107803077), Pyruvate kinase (LOC107822892), Pyruvate kinase (LOC107783505), Pyruvate kinase (LOC107827388), Pyruvate kinase (LOC107782568), Pyruvate kinase (LOC107777547), Pyruvate kinase, Pyruvate kinase isozyme G, chloroplastic, Pyruvate kinase isozyme A, chloroplastic, Pyruvate kinase (LOC107799668), Pyruvate kinase (LOC107773422), Pyruvate kinase (LOC107787620), Pyruvate kinase (LOC107818508), Pyruvate kinase (LOC107794535), Pyruvate kinase (LOC107760370), Pyruvate kinase (LOC107782907), Pyruvate kinase (PKTL7), Pyruvate kinase (LOC107818508)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 28 | Manganese 2By similarity | 1 | |
Active sitei | 81 | Phosphoserine intermediateBy similarity | 1 | |
Metal bindingi | 81 | Manganese 2By similarity | 1 | |
Binding sitei | 140 | SubstrateBy similarity | 1 | |
Binding sitei | 206 | SubstrateBy similarity | 1 | |
Binding sitei | 213 | SubstrateBy similarity | 1 | |
Binding sitei | 361 | SubstrateBy similarity | 1 | |
Metal bindingi | 430 | Manganese 1By similarity | 1 | |
Metal bindingi | 434 | Manganese 1By similarity | 1 | |
Metal bindingi | 471 | Manganese 2By similarity | 1 | |
Metal bindingi | 472 | Manganese 2By similarity | 1 | |
Metal bindingi | 501 | Manganese 1By similarity | 1 |
GO - Molecular functioni
- 2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity Source: GO_Central
- manganese ion binding Source: GO_Central
GO - Biological processi
- cellular carbohydrate metabolic process Source: GO_Central
- glucose catabolic process Source: InterPro
- glycolytic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Isomerase |
Biological process | Glycolysis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00186 |
Names & Taxonomyi
Protein namesi | Recommended name: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC:5.4.2.12)Short name: BPG-independent PGAM Short name: Phosphoglyceromutase Alternative name(s): PGAM-I |
Organismi | Nicotiana tabacum (Common tobacco) |
Taxonomic identifieri | 4097 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana |
Proteomesi |
|
Subcellular locationi
Other locations
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212114 | 1 – 559 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutaseAdd BLAST | 559 |
Proteomic databases
PRIDEi | P35494 |
ProMEXi | P35494 |
Expressioni
Tissue specificityi
Found ubiquitously in germinating seed.
Developmental stagei
Expression most important during germination, it decreases during development.
Interactioni
Subunit structurei
Monomer.
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 170 – 171 | Substrate bindingBy similarity | 2 | |
Regioni | 286 – 289 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the BPG-independent phosphoglycerate mutase family.Curated
Family and domain databases
CDDi | cd16010, iPGM, 1 hit |
Gene3Di | 3.40.1450.10, 1 hit 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR011258, BPG-indep_PGM_N IPR006124, Metalloenzyme IPR036646, PGAM_B_sf IPR005995, Pgm_bpd_ind |
PANTHERi | PTHR31637, PTHR31637, 1 hit |
Pfami | View protein in Pfam PF06415, iPGM_N, 1 hit PF01676, Metalloenzyme, 1 hit |
PIRSFi | PIRSF001492, IPGAM, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit SSF64158, SSF64158, 1 hit |
TIGRFAMsi | TIGR01307, pgm_bpd_ind, 1 hit |
i Sequence
Sequence statusi: Complete.
P35494-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSSGDAWKL KDHPKLPKGK TVAVIVLDGW GEAKPNEFNA IHVAETPVMY
60 70 80 90 100
SLKNGAPEKW RLIKAHGNAV GLPTEDDMGN SEVGHNALGA GRIFAQGAKL
110 120 130 140 150
VDLALASGKI YEGEGFKYVK ECFEKGTLHL IGLLSDGGVH SRLDQVQLLL
160 170 180 190 200
KGAAKHGAKR IRVHALTDGR DVLDGSSVGF METLENSLAQ LREKGIDAQV
210 220 230 240 250
ASGGGRMYVT MDRYENDWDV VKRGWDAQVL GEAPHKFKDP VEAVKKLRQE
260 270 280 290 300
PNANDQYLAP FVIVDDNGKP VAAILDGDAV VTFNFRADRM VMLAKALEYE
310 320 330 340 350
NFDKFDRVRV PKIRYAGMLQ YHGELQLPSH YLVSPPEIAR HSGEYLVRNG
360 370 380 390 400
VRTFACSETV KFGHVTFFWN GNRSGYFNEK LEEYVEIPSD SGITFNVKPK
410 420 430 440 450
MKALEIAERT RDAILSGKFD QVRVNLPNGD MVGHTGDIKA TIEACKSADE
460 470 480 490 500
AVKMILEAIE QVGGIYLVTA DHGNAEDMVK RNKKGEPALD KNGNIQILTS
510 520 530 540 550
HTCEPVPIAI GGPGLAPGVR FRQDLPTGGL ANVAATFMNL HGSEAPSDYE
PSLIEVVDN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70651 mRNA Translation: CAA49994.1 |
PIRi | S44373 |
RefSeqi | NP_001311960.1, NM_001325031.1 |
Genome annotation databases
GeneIDi | 107769473 |
KEGGi | nta:107769473 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70651 mRNA Translation: CAA49994.1 |
PIRi | S44373 |
RefSeqi | NP_001311960.1, NM_001325031.1 |
3D structure databases
SMRi | P35494 |
ModBasei | Search... |
Proteomic databases
PRIDEi | P35494 |
ProMEXi | P35494 |
Genome annotation databases
GeneIDi | 107769473 |
KEGGi | nta:107769473 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00186 |
Family and domain databases
CDDi | cd16010, iPGM, 1 hit |
Gene3Di | 3.40.1450.10, 1 hit 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR011258, BPG-indep_PGM_N IPR006124, Metalloenzyme IPR036646, PGAM_B_sf IPR005995, Pgm_bpd_ind |
PANTHERi | PTHR31637, PTHR31637, 1 hit |
Pfami | View protein in Pfam PF06415, iPGM_N, 1 hit PF01676, Metalloenzyme, 1 hit |
PIRSFi | PIRSF001492, IPGAM, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit SSF64158, SSF64158, 1 hit |
TIGRFAMsi | TIGR01307, pgm_bpd_ind, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMGI_TOBAC | |
Accessioni | P35494Primary (citable) accession number: P35494 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families